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8 results for “Heterosigma akashiwo”
Protocols and data for Heterosigma akashiwo transformation approaches
<p>This dataset provides information about plasmid construction, antibiotic sensitivity and transformation approaches for the algal species, <em>Heterosigma akashiwo</em>.</p> <p><strong>Note that protocols for glass bead-mediated transformation and electroporation of <em>Heterosigma akashiwo</em> have been updated. Please see http://dx.doi.org/10.17504/protocols.io.hjkb4kw and http://dx.doi.org/10.17504/protocols.io.hjmb4k6.</strong></p>
Data for: Heterosigma akashiwo transcriptome gene annotations
<p>Heterosigma akashiwo is a eukaryotic, cosmopolitan, and unicellular alga (class: Raphidophyceae), and produces fish-killing blooms. There is a substantial scientific and practical interest in its ecophysiological characteristics that determine bloom dynamics and its adaptation to broad climate zones. A well-annotated genomic/genetic sequence information enables researchers to characterize organisms using modern molecular technology. In the present study, we conducted H. akashiwo RNA sequencing, a de novo transcriptome assembly of 84,693,530 high-quality deduplicated short-read sequences. The obtained RNA reads were assembled by Trinity assembler and 144,777 contigs were identified with N50 values of 1085. The raw data were deposited in the NCBI SRA database (BioProject PRJDB6241 and PRJDB15108), and the assemblies are available in NCBI TSA database (ICRV01). Total 60,877 open reading frames with the length of 150 bp or greater were predicted. Here, the top Gene Ontology terms, the pfam hits, and the BLAST hits were annotated for all the predicted genes, and shared as text files.</p>
Data for: Heterosigma akashiwo transcriptome gene annotations
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Effects of nitrate and ammonium on assimilation of nitric oxide by Heterosigma akashiwo
<p>This data set includes 15-NO uptake rates, gene expression of NR, GOGAT and GS, and nitrate reductase activity for the raphidophyte <em>Heterosigma akashiwo</em>.</p> <p>For details, see Healey, E.M., Flood, S., Bock, P.K., Fulwieler, R.W., York, J.K. and Coyne, K.J. (2023) Effects of nitrate and ammonium on assimilation of nitric oxide by <em>Heterosigma akashiwo</em>. <em>Sci Rep</em> 13, 621. DOI: 10.1038/s41598-023-27692-3. </p> <p><strong>Funding source</strong>: US National Oceanic and Atmospheric Association (NOAA) National Centers for Coastal Ocean Science (NCCOS) grant number <span>NA18NOS4780165; ECOHAB 2017: The role of nitric oxide in promoting Heterosigma blooms.</span></p>
Data from: A hypervariable mitochondrial protein coding sequence associated with geographical origin in a cosmopolitan bloom-forming alga, Heterosigma akashiwo
Geographic distributions of phytoplankton species can be defined by events on both evolutionary time and shorter scales, e.g., recent climate changes. Additionally, modern industrial activity, including the transport of live fish and spat for aquaculture and aquatic microorganisms in ship ballast water, may aid the spread of phytoplankton. Obtaining a reliable marker is key to gaining insight into the phylogeographic history of a species. Here, we report a hypervariable mitochondrial gene in the cosmopolitan bloom-forming alga, Heterosigma akashiwo. We compared the entire mitochondrial genome sequences of seven H. akashiwo strains from Japanese and North American coastal waters and identified a hypervariable segment. The region codes for a hypothetical protein with no defined function, and its variations between Japanese and North American isolates, were prominent, while the sequences were more conserved among Japanese strains and North American isolates. Comparison of the sequence in isolates obtained from different geographical points in the Northern Hemisphere revealed that the sequence variations largely correlated with latitude and longitude (i.e. Pacific/Atlantic oceans). Our results demonstrate the usefulness of the sequence in determining the phylogeographic history of H. akashiwo.
Dataset generated in support of nitric oxide assimilation by Heterosigma akashiwo when acclimated to growth on nitrate, ammonium, or a mix of nitrate and ammonium
<p>This dataset includes primary data generated during our investigation of the effects of competing nitrogen sources on the assimilation of nitric oxide (NO) into algal biomass by the harmful algal species, <em>Heterosigma akashiwo</em>. <em>H. akashiwo </em>was grown in continuous culture on artificial seawater and provided with either nitrate, ammonium or nitrate plus ammonium as a sole source of nitrogen. Cultures were spiked with NO (100 µM), or NO<sub>3</sub><sup>-</sup>, or NH<sub>4</sub><sup>+ </sup>at concentrations equal to 10% ambient levels. Controls were spiked with an equal volume of water. Samples were collected for RT-qPCR analysis of gene expression at 15 and 60 minutes after spiking. Additional samples were collected for analysis of NR enzyme activity at 2 hours after spiking, and again at 4 hours and 24 hours to evaluate assimilation of NO into biomass.</p>
Data from: A hypervariable mitochondrial protein coding sequence associated with geographical origin in a cosmopolitan bloom-forming alga, Heterosigma akashiwo
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Ocean warming and acidification affect the transitional C:N:P ratio and macromolecular accumulation in the harmful raphidophyte Heterosigma akashiwo
GEO Series GSE223198. Heterosigma akashiwo. 6 samples. Type: Expression profiling by high throughput sequencing.
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