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4 results for “Hoechst”
An annotated high-content fluorescence microscopy dataset with Hoechst 33342-stained nuclei and manually labelled outlines
<p>Here we present a benchmarking dataset of fluorescence microscopy images with Hoechst 33342-stained nuclei together with annotations of nuclei, nuclear fragments and micronuclei. Images were randomly selected from an RNA interference screen with a modified U2OS osteosarcoma cell line, acquired on a Thermo Fischer CX7 high-content imaging system at 20x magnification. Labelling was performed by a single annotator and reviewed by a biomedical expert.</p> <p>The dataset contains 50 images showing over 2000 labelled nuclear objects in total, which is sufficiently large to train well-performing neural networks for instance or semantic segmentation. It is pre-split into training, development and test set, each in a zip file. The dataset should be referred to as Aitslab_bioimaging1. A brief article describing the dataset is also available (Arvidsson M, Kazemi Rashed S, Aits S. <a href="https://doi.org/10.1016/j.dib.2022.108769">10.1016/j.dib.2022.108769</a> )</p> <p><strong>Dataset description:</strong></p> <p>Fluorescence microscopy images: original .C01 files and files converted to 8-bit .png format (Grayscale)</p> <p>Annotations: 24-bit .png format (RGB)</p> <p>Script used to convert C01 to png images: C01_to_png.py file with python code and readme.md file with instructions to run it</p>
C2C12 and THP1 images Brightfield and Hoechst
<p>20221207_1700_gt_C2C12 has 2x 96 TIF files with Brightfield and Hoechst stained images of C2C12 cells plated in 96 well plates the day before imaging</p> <p>20231106_1300_mm_THP1 has 2x 96 TIF files with Brightfield and Hoechst stained images of THP1 cells cultured in 96 well plates for two days in 100ng/ml PMA, and 1 day in routine culture meidum before imaging.</p> <p><span>Wells of the 96-well plates were imaged automatically using a Cytation-1 Cell imaging multimode reader (Agilent) set at 37 degrees Celsius using the Gen5 (version 3.12) software. For brightfield images, one image per well was captured with an Olympus 4x UPLFLN objective, using user-trained auto-focus and manual exposure settings that were set before imaging of each plate. For nuclei counting, 4 </span><span><span>m</span></span><span>M</span><span> of Hoechst (33342, #B2261, Sigma) from a 40 </span><span><span>m</span></span><span>M</span><span> stock solution was added 15 minutes before imaging to each well. Nuclei image fluorescence readings were taken using the same Olympus 4x UPLFLN objective and a 365 nm LED with an EX337/EM447 DAPI filter cube.</span></p> <p> </p>
Differential gene expression profiles induced by Hoechst dyes
GEO Series GSE28616. Homo sapiens. 3 samples. Type: Expression profiling by array.
Gene expression profiling and localization of Hoechst-effluxing CD45- and CD45+ cells in the Embryonic Mouse Lung
GEO Series GSE2057. Mus musculus. 14 samples. Type: Expression profiling by array.
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