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78 results for “Horizontal transfer”
Supplementary phylogenetic data for Manzano-Marín et. al. 2020 "Serial horizontal transfer of vitamin-biosynthetic genes enables the establishment of new nutritional symbionts in aphids' di-symbiotic systems"
<p>Supplementary data for Manzano-Marín et. al. 2019 "Serial horizontal transfer of vitamin-biosynthetic genes enables the establishment of new nutritional symbionts in aphids' di-symbiotic systems".</p> <p>The data set consists of four folders:</p> <p>1) "Buchnera_phylo”: PHYLIP-formatted file used for phylogenetic reconstruction of <em>Buchnera</em> and resulting tree in NEWICK format.</p> <p>2) "Erwinia_phylo”: PHYLIP-formatted file used for phylogenetic reconstruction of <em>Erwinia</em> and resulting tree in NEWICK format.</p> <p>3) "Hamiltonella_phylo”: FASTA-formatted nucleotide alignment files of each gene and NEXUS-formatted files used for Bayesian phylogenetic reconstruction of <em>Hamiltonella</em> symbionts.</p> <p>4) "HGT_genes": FASTA-formatted nucleotide alignment files of each horizontally transferred gene and non-horizontally transferred genes nupC, and <em>gpmA</em>. Also, NEXUS-formatted files used for Bayesian phylogenetic reconstruction and of resulting trees.</p> <p>5) "Tn3_pylo": FASTA-formatted amino acid alignment files of mobile elements related to the Tn3 family resolvase/invertase found in <em>Hamiltonella</em>-associated <em>Erwinia haradaeae</em> symbionts. Also, NEXUS-formatted files used for Bayesian phylogenetic reconstruction and of resulting trees.</p>
No evidence for extensive horizontal gene transfer in the genome of the tardigrade Hypsibius dujardini
<p><strong>No evidence for extensive horizontal gene transfer in the genome of the tardigrade Hypsibius dujardini</strong></p> <p>These files accompany the peer-reviewed version of http://dx.doi.org/10.1101/033464</p> <p>A previous dataset https://zenodo.org/record/45162 accompanied the version of this manuscript at BioRxiv - biorxiv.org/content/early/2015/12/13/033464</p> <p>This dataset includes all files from https://zenodo.org/record/45162 plus all the Supplemental files, and one additional file HGT_phylogenetic_files.tgz. All files are described in Hypsibius_dujardini_files_README.md</p> <p><strong>Abstract</strong></p> <p>Tardigrades are meiofaunal ecdysozoans that are key to understanding the origins of Arthropoda. Many species of Tardigrada can survive extreme conditions through cryptobiosis. In a recent paper (Boothby TC <em>et al </em>(2015) Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade. <em>Proc Natl Acad Sci USA</em> 112:15976-15981) the authors concluded that the tardigrade <em>Hypsibius dujardini </em>had an unprecedented proportion (17%) of genes originating through functional horizontal gene transfer (fHGT), and speculated that fHGT was likely formative in the evolution of cryptobiosis. We independently sequenced the genome of <em>H. dujardini</em>. As expected from whole-organism DNA sampling, our raw data contained reads from non-target genomes. Filtering using metagenomics approaches generated a draft <em>H. dujardini</em> genome assembly of 135 Mb with superior assembly metrics to the previously published assembly. Additional microbial contamination likely remains. We found no support for extensive fHGT. Among 23,021 gene predictions we identified 0.2% strong candidates for fHGT from bacteria, and 0.2% strong candidates for fHGT from non-metazoan eukaryotes. Cross-comparison of assemblies showed that the overwhelming majority of HGT candidates in the Boothby <em>et al.</em> genome derived from contaminants. We conclude that fHGT into <em>H. dujardini </em>accounts for at most 1-2% of genes and that the proposal that one sixth of tardigrade genes originate from functional HGT events is an artefact of undetected contamination.</p> <p> </p>
Comparative genomics of eight aphid subfamilies reveals variable relationships between host horizontally-transferred genes and symbiont peptidoglycan metabolism.
<p>Genome assemblies, annotations, and orthologs of aphids (<em>Geopemphigus sp.</em>, <em>Stegophylla sp.</em>, <em>Chaitophorus viminalis</em>, and<em> Pemphigus obesinymphae</em>) and their symbionts. </p> <p>step1_final_assemblies_and_annotations.tar.gz: Aphid genomes and annotations</p> <p>step2_protein_evidence_used_for_genome_annotation.tar.gz: Protein evidence used for aphid genome annotation</p> <p>step3_amino_acid_inputs_for_aphid_orthologs: amino acid inputs for aphid ortholog assignmentt</p> <p>step5_buchnera_genomes_and_annotations.tar.gz: Buchnera genomes and annotations</p>
Part 1: Dataset and script for a manuscript entitled 'Host-specific subtelomere: structural variation and horizontal transfer in asexual filamentous fungal pathogens'
<p>Datasets, scripts and instructions for reproducing some of the results in the manuscript. The file subtelomere.tar needs to be unpacked on a Linux system. After unpacking it, go to the directory subtelomere, which contains a number of subdirectories. One subdirectory is named data, which contains genome assemblies and is used to hold datasets of short reads; the datasets of short reads in the files Data.One.Focb.tar, Data.One.Focb-2.tar and reads.tar on the four-part depository need to be placed in the subdirectory subtelomere/data/reads/. The other subdirectories under the directory subtelomere contain instructions and scripts for reproducing some of the results in the manuscript. Please see the README and z.cmd files in each subdirectory.</p> <p>The file Data.One.Focb-2.tar contains 22 files of paired-end reads from F. oxysporum f.sp. cubense tropical race 1 isolate N2 (SRA accession: SRR550150, SRR550151), and F. oxysporum f.sp. cubense TR4 isolates Hainan.B2 (SRR550152), My-1 (SRR7226877), La-2 (SRR7226878), Vn-2 (SRR7226879), Leb1.2C (SRR7226880), JV11 (SRR7226881), Phi2.6C (SRR7226882), Pak1.1A (SRR7226883), UK0001 (SRR9733598).</p>
Part 2: Dataset and script for a manuscript entitled 'Host-specific subtelomere: structural variation and horizontal transfer in asexual filamentous fungal pathogens'
<p>Datasets, scripts and instructions for reproducing some of the results in the manuscript. The file subtelomere.tar needs to be unpacked on a Linux system. After unpacking it, go to the directory subtelomere, which contains a number of subdirectories. One subdirectory is named data, which contains genome assemblies and is used to hold datasets of short reads; the datasets of short reads in the files Data.One.Focb.tar, Data.One.Focb-2.tar and reads.tar on the four-part depository need to be placed in the subdirectory subtelomere/data/reads/. The other subdirectories under the directory subtelomere contain instructions and scripts for reproducing some of the results in the manuscript. Please see the README and z.cmd files in each subdirectory.</p> <p>The file Data.One.Focb.tar contains 16 files of paired-end reads from F. oxysporum f.sp. cubense TR4 isolates II-5 (SRA accession: SRR10054446), S1B8 (SRR10054447), JV14 (SRR10054448), FOC.TR4-5 (SRR10054449), FOC.TR4-1 (SRR10054450), Col2 (SRR10103605), Col4 (SRR10125423), Col17 (SRR10747097).</p> <p> </p>
Part 4: Dataset and script for a manuscript entitled 'Host-specific subtelomere: structural variation and horizontal transfer in asexual filamentous fungal pathogen
<p>Datasets, scripts and instructions for reproducing some of the results in the manuscript. The file subtelomere.tar needs to be unpacked on a Linux system. After unpacking it, go to the directory subtelomere, which contains a number of subdirectories. One subdirectory is named data, which contains genome assemblies and is used to hold datasets of short reads; the datasets of short reads in the files Data.One.Focb.tar, Data.One.Focb-2.tar and reads.tar on the four-part depository need to be placed in the subdirectory subtelomere/data/reads/. The other subdirectories under the directory subtelomere contain instructions and scripts for reproducing some of the results in the manuscript. Please see the README and z.cmd files in each subdirectory.</p> <p>The file reads.tar contains 44 files of paired-end reads from <em>F. oxysporum </em>f.sp. <em>lycopersici</em> isolate Fol069 (SRA accession: SRR307106, SRR307107, SRR307113, SRR307115, SRR307123, SRR307257, SRR307266), isolate Fol072 (SRR307122, SRR307092, SRR307091, SRR307090, SRR307086, SRR307281, SRR307250), isolate Fol4287 (SRR7690004, SRR3139043), and F. oxysporum f.sp. radicis-cucumerinum isolate Forc016 (SRR3139027, SRR3139028), isolate Forc024 (SRR3139029, SRR3139030), isolate Forc031 (SRR3139031, SRR3139032).</p>
Data from: Comparative genomics reveals high rates of horizontal transfer and strong purifying selection on rhizobial symbiosis genes
<p class="western"><span>Horizontal transfer (HT) alters the repertoire of symbiosis genes in rhizobial genomes and may play an important role in the on-going evolution of the rhizobia-legume symbiosis. To gain insight into the extent of HT of symbiosis genes with different functional roles (nodulation, N-fixation, host benefit, and symbiont fitness), we conducted comparative genomic and selection analyses of the full genome sequences from 27 rhizobial genomes. We find that symbiosis genes experience high rates of HT among rhizobial lineages but also bear signatures of purifying selection (low Ka:Ks). HT and purifying selection appear to be particularly strong in genes involved in initiating the symbiosis (e.g. nodulation) and in genome-wide association candidates for mediating variation in benefits provided to the host. These patterns are consistent with rhizobia adapting to the host environment through the loss and gain of symbiosis genes, but not with host-imposed positive selection driving divergence of symbiosis genes through recurring bouts of positive selection.</span></p>
Experimental Investigation of the Heat Transfer between Finned Tubes and a Bubbling Fluidized Bed with Horizontal Sand Mass Flow
<p>Data repository for the paper:</p> <p>Thanheiser, S.; Haider, M.; Schwarzmayr, P. Experimental Investigation of the Heat Transfer between Finned Tubes and a Bubbling Fluidized Bed with Horizontal Sand Mass Flow. Energies 2022, 15, 1316. https://doi.org/10.3390/en15041316</p>
Dataset from: Horizontal transfer of BovB and L1 retrotransposons in eukaryotes
<p><strong>Background: </strong>Transposable elements are mobile DNA sequences, colloquially known as jumping genes because of their ability to replicate to new genomic locations. TEs can jump between organisms or species when given a vector of transfer, such as a tick or a virus, in a process known as horizontal transfer. Here, we propose that LINE-1(L1) and Bovine-B (BovB), the two most abundant transposable element families in mammals, were initially introduced as foreign DNA via ancient horizontal transfer events.</p> <p><strong>Results: </strong>Using analyses of over 759 plant, fungal and animal genomes, we identify multiple possible L1 horizontal transfer events in eukaryotic species, primarily involving Tx-like L1s in marine eukaryotes. We also extend the BovB paradigm by increasing the number of estimated transfer events compared to previous studies, finding new parasite vectors of transfer such as bed bug, leech, and locust, and BovB occurrences in new lineages such as bat and frog. Given that these transposable elements have colonized more than half of the genome sequence in today's mammals, our results support a role for horizontal transfer in causing long-term genomic change in new host organisms.</p> <p><strong>Conclusions:</strong> We describe extensive horizontal transfer of BovB retrotransposons and provide the first evidence that L1 elements can also undergo horizontal transfer. With the advancement of genome sequencing technologies and bioinformatics tools, we anticipate our study to be a valuable resource for inferring horizontal transfer from large-scale genomic data.</p> <p><strong>Dataset:</strong> The deposited dataset contains the identified TE sequences (L1 and BovB) from all genomes and the putative horizontal transfer clusters described in the text. See Additional File 1 (Tables S1-6) and Additional File 2 (Figures S1-55) for detailed descriptions of the sequences and clusters.</p> <p> </p>
Processed data for Evidence of horizontal gene transfer and environmental selection impacting antibiotic resistance evolution in soil-dwelling Listeria
<p>Processed/source data for the manuscript Evidence of horizontal gene transfer and environmental selection impacting antibiotic resistance evolution in soil-dwelling <em>Listeria</em>.</p>
Horizontal transfer and subsequent explosive expansion of a DNA transposon in sea kraits (Laticauda)
<p><strong>Abstract</strong></p> <p>Transposable elements (TEs) are self replicating genetic sequences and are often described as important “drivers of evolution”. This driving force is because TEs promote genomic novelty by enabling rearrangement, and through exaptation as coding and regulatory elements. However, most TE insertions will be neutral or harmful, therefore host genomes have evolved machinery to supress TE expansion. Through horizontal transposon transfer (HTT) TEs can colonise new genomes, and since new hosts may not be able to shut them down, these TEs may proliferate rapidly. Here we describe HTT of the <em>Harbinger-Snek</em> DNA transposon into sea kraits (<em>Laticauda</em>), and its subsequent explosive expansion within <em>Laticauda</em> genomes. This HTT occurred following the divergence of <em>Laticauda</em> from terrestrial Australian elapids ~15-25 Mya. This has resulted in numerous insertions into introns and regulatory regions, with some insertions into exons which appear to have altered UTRs or added sequence to coding exons. <em>Harbinger-Snek</em> has rapidly expanded to make up 8-12% of <em>Laticauda</em> spp. genomes; this is the fastest known expansion of TEs in amniotes following HTT. Genomic changes caused by this rapid expansion may have contributed to adaptation to the amphibious-marine habitat.</p> <p><strong>Dataset</strong></p> <p>The deposited dataset contains scripts used in analysis, GFFs of the <em>Laticauda </em>genome gene annotations produced using Liftoff, repeat sequences of all <em>Harbinger-Snek variants and Harbinger-Snek</em>-like TEs, repeat library used in RepeatMasker repeat annotation, repeat annotation of <em>Laticauda, Notechis</em> and <em>Pseudonaja</em> genomes, screenshots of IGV showing RNASeq reads mapped to gene exons and UTRs containing <em>Harbinger-Snek</em> insertions, and all phylogenetic trees and the sequence data used in generating them.</p>
Genome assemblies: Horizontal transfer of pOXA-48 from a hypervirulent Klebsiella pneumoniae ST23/KL57 to Serratia marcescens
<p>Hypervirulent Klebsiella pneumonia isolates express a range of virulence factors, often encoded on virulence plasmids. Occasionally these hypervirulent lineages acquire antimicrobial resistance genes rendering them multidrug-resistant. We describe the nosocomial transmission of a hypervirulent K. pneumoniae ST23/KL57 isolate carrying an NDM-1 and OXA-48 beta-lactamase among COVID-19 patients in a Danish university hospital. Furthermore, we characterize the plasmid structure and describe a within-patient horizontal transfer of a plasmid carrying a blaOXA-48 gene from K. pneumoniae ST23/KL57 to a Serratia marcescens isolate.</p>
Dataset from: The origin and fate of fungal mitochondrial horizontal gene transferred sequences in orchids (Orchidaceae)
<p>The transfer of DNA among distantly related organisms is relatively common in bacteria but less prevalent in eukaryotes. Among fungi and plants, few of these events have been reported. Two segments of fungal mitochondrial DNA have been discovered in the mitogenome of orchids. Here, we build on their work to understand the timing of those transfer events, which orchids retain the fungal DNA, and the fate of the foreign DNA during orchid evolution. We update the content of the large DNA fragment and establish that it was transferred to the most recent common ancestor of a highly diverse clade of epidendroid orchids that lived ~28–43 Mya. Also, we present hypotheses of the origin of the small transferred fragment. Our findings deepen the knowledge of these interesting DNA transfers among organelles and we formulate a probable mechanism for these horizontal gene transfer events.</p>
Data from: Comparative genomics reveals high rates of horizontal transfer and strong purifying selection on rhizobial symbiosis genes
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Dataset from: The origin and fate of fungal mitochondrial horizontal gene transferred sequences in orchids (Orchidaceae)
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Data from: Experimental horizontal transfer of phage-derived genes to Drosophila confers innate immunity to parasitoids
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Research data supporting article "Recurrent horizontal transfer identifies mitochondrial positive selection in a transmissible cancer"
<p>Gene expression data supporting analyses described in the article "Recurrent horizontal transfer identifies mitochondrial positive selection in a transmissible cancer" (Strakova et al., 2020).</p>
Data from: De novo gene birth, horizontal gene transfer and gene duplication as sources of new gene families associated with the origin of a symbiosis in Amanita
<p>By introducing novel capacities and functions, new genes and gene families may play a crucial role in ecological transitions. Mechanisms generating new gene families include <i>de novo</i> gene birth, horizontal gene transfer and neofunctionalization following a duplication event. The ectomycorrhizal (ECM) symbiosis is a ubiquitous mutualism and the association has evolved repeatedly and independently many times among the fungi, but the molecular dynamics enabling its emergence remain elusive. We developed a phylogenetic workflow to first understand if gene families unique to ECM <i>Amanita</i> fungi and absent from closely related asymbiotic species are functionally relevant to the symbiosis, and then to systematically infer their origins. We identified 109 gene families unique to ECM <i>Amanita </i>species. Genes belonging to unique gene families are under strong purifying selection and are upregulated during symbiosis, compared to genes of conserved or orphan gene families. The origins of seven of the unique gene families are strongly supported as either <i>de novo</i> gene birth (two gene families), horizontal gene transfer (four), and gene duplication (one). An additional 34 families appear new because of their selective retention within symbiotic species. Among the 109 unique gene families, the most upregulated gene in symbiotic cultures encodes an ACC deaminase, an enzyme capable of downregulating the synthesis of the plant hormone ethylene. Ethylene is a common negative regulator of plant-microbial mutualisms.</p>
Data from: Establishment and maintenance of aphid endosymbionts after horizontal transfer is dependent on host genotype
Animal-associated microbial communities have important effects on host phenotypes. Individuals within and among species differ in the strains and species of microbes that they harbour, but how natural selection shapes the distribution and abundance of symbionts in natural populations is not well understood. Symbionts can be beneficial in certain environments but also impose costs on their hosts. Consequently, individuals that can or cannot associate with symbionts will be favoured under different ecological circumstances. As a result, we predict that individuals within a species vary in terms of how well they accept and maintain symbionts. In pea aphids, the frequency of endosymbionts varies among host-plant-associated populations ('biotypes'). We show that aphid genotypes from different biotypes vary in how well they accept and maintain symbionts after horizontal transfer. We find that aphids from biotypes that frequently harbour symbionts are better able to associate with novel symbionts than those from biotypes that less frequently harbour symbionts. Intraspecific variation in the ability of hosts to interact with symbionts is an understudied factor explaining patterns of host–symbiont association.
Data from: Ecological overlap and horizontal gene transfer in Staphylococcus aureus and Staphylococcus epidermidis
The opportunistic pathogens Staphylococcus aureus and Staphylococcus epidermidis represent major causes of severe nosocomial infection, and are associated with high levels of mortality and morbidity worldwide. These species are both common commensals on the human skin and in the nasal pharynx, but are genetically distinct, differing at 24% average nucleotide divergence in 1,478 core genes. To better understand the genome dynamics of these ecologically similar staphylococcal species, we carried out a comparative analysis of 324 S. aureus and S. epidermidis genomes, including 83 novel S. epidermidis sequences. A reference pan-genome approach and whole genome multilocus-sequence typing revealed that around half of the genome was shared between the species. Based on a BratNextGen analysis, homologous recombination was found to have impacted on 40% of the core genes in S. epidermidis, but on only 24% of the core genes in S. aureus. Homologous recombination between the species is rare, with a maximum of nine gene alleles shared between any two S. epidermidis and S. aureus isolates. In contrast, there was considerable interspecies admixture of mobile elements, in particular genes associated with the SaPIn1 pathogenicity island, metal detoxification, and the methicillin-resistance island SCCmec. Our data and analysis provide a context for considering the nature of recombinational boundaries between S. aureus and S. epidermidis and, the selective forces that influence realized recombination between these species.
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