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638 results for “Human neurons”
Dataset of neurons and intracranial EEG from human amygdala during aversive dynamic visual stimulation
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Dataset Activation of Lactate Receptor HCAR1 Down-modulates Neuronal Activity in Rodent and Human Brain Tissue
<p>This dataset is related to the study: </p> <p>Briquet M, Rocher AB, Alessandri M, Rosenberg N, de Castro Abrantes H, Wellbourne-Wood J, Schmuziger C, Ginet V, Puyal J, Pralong E, Daniel RT, Offermanns S, Chatton JY. Activation of lactate receptor HCAR1 down-modulates neuronal activity in rodent and human brain tissue. J Cereb Blood Flow Metab. 2022 Mar 3:271678X221080324. doi: 10.1177/0271678X221080324. Epub ahead of print. PMID: 35240875.</p>
The Human Developing Cerebral Cortex Is Characterized by an Elevated De Novo Expression of Long Noncoding RNAs in Excitatory Neurons
<p>This project contains the annotated transcriptomes in GTF format used in the manuscript "The Human Developing Cerebral Cortex Is Characterized by an Elevated De Novo Expression of Long Noncoding RNAs in Excitatory Neurons" DOI: <a href="https://doi.org/10.1093/molbev/msae123">https://doi.org/10.1093/molbev/msae123</a></p>
Chronic exposure to glucocorticoids amplifies inhibitory neuron cell fate during human neurodevelopment in organoids
<p><strong>Abstract: </strong>Disruptions in the tightly regulated process of human brain development have been linked to increased risk for brain and mental illnesses. While the genetic contribution to these diseases is well established, important environmental factors have been less studied at molecular and cellular levels. In this study, we used single-cell and cell-type-specific techniques to investigate the effect of glucocorticoid (GC) exposure, a mediator of antenatal environmental risk, on gene regulation and lineage specification in unguided human neural organoids. We characterized the transcriptional response to chronic GC exposure during neural differentiation and studied the underlying gene regulatory networks by integrating single-cell transcriptomics- with chromatin accessibility data. We found lasting cell type-specific changes that included autism risk genes and several transcription factors associated with neurodevelopment. Chronic GCs influenced lineage specification primarily by priming the inhibitory neuron lineage through key transcription factors like PBX3. We provide evidence for convergence of genetic and environmental risk factors through a common mechanism of altering lineage specification.</p>
Genetic polymorphisms in COMT and BDNF influence synchronization dynamics of human neuronal oscillations
<p>Neuronal oscillations, their inter-areal synchronization, and scale-free dynamics constitute fundamental mechanisms for cognition by regulating communication in neuronal networks. These oscillatory dynamics have large inter-individual variability that is partly heritable. We hypothesized that this variability could be partially explained by genetic polymorphism in neuromodulatory genes. We recorded resting-state magnetoencephalography (MEG) from 82 healthy participants and investigated whether oscillation dynamics were influenced by genetic polymorphisms in Catechol-O-methyltransferase (COMT) Val<sup>158</sup>Met and brain-derived neurotrophic factor (BDNF) Val<sup>66</sup>Met. Both COMT and BDNF polymorphisms influenced local oscillation amplitudes and their long-range temporal correlations (LRTCs), while only BDNF polymorphism affected the strength of large-scale synchronization. Our findings demonstrate that COMT and BDNF genetic polymorphisms contribute to inter-individual variability in neuronal oscillation dynamics. Comparison of these results to computational modeling of near-critical synchronization dynamics further suggested that COMT and BDNF polymorphisms influenced local oscillations by modulating the excitation-inhibition balance according to the brain criticality framework.</p>
Large-scale neural recordings with single neuron resolution using Neuropixels probes in human cortex
<p><span>Recent advances in multi-electrode array technology have made it possible to monitor large neuronal ensembles at cellular resolution in animal models. In humans, however, c</span>urrent approaches restrict recordings to few neurons per penetrating electrode or combine the signals of thousands of neurons in local field potential (LFP) recordings. Here, we describe a new probe variant and set of techniques which enable simultaneous recording from over 200 well-isolated cortical single units in human participants during intraoperative neurosurgical procedures using silicon Neuropixels probes. We characterized a diversity of extracellular waveforms with eight separable single unit classes, with differing firing rates, locations along the length of the electrode array, waveform spatial spread, and modulation by LFP events such as inter-ictal discharges and burst suppression. While some challenges remain in creating a turn-key recording system, high-density silicon arrays provide a path for studying human-specific cognitive processes and their dysfunction at unprecedented spatiotemporal resolution. </p>
Raw image files in Fig. S1E associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains raw ND2 files shown in Fig. S1E associated with: https://doi.org/10.1101/2024.09.24.614704</p>
Raw image files of uncropped western blots associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains uncropped blots for Fig. 1C, S1C, S1D, 2I, and S3B for: https://doi.org/10.1101/2024.09.24.614704</p>
Data supporting "Changes in human dorsal root ganglion neuron excitability from modulating Nav 1.8 conductance are non-linear and depend on the conductances of the delayed rectifier and M-type potassium currents: a simulation study"
<p>Data and code supporting the article "Changes in human dorsal root ganglion neuron excitability from modulating Nav 1.8 conductance are non-linear and depend on the conductances of the delayed rectifier and M-type potassium currents: a simulation study".</p> <p> </p>
RNAseq data: Analysis of circRNA expression in human neuronal differentiation
<p>This dataset contains sequencing read count data related to samples from differentiating human neuroepithelial stem cells (NES) collected at days zero (NES), five (D5) and 28 (D28) of differentiation. Details on how samples were collected and how data was generated and processed are described below.</p> <p> </p> <p><em>Sample preparation</em></p> <p>NES were seeded on tissue culture flasks coated with 20 μg/ml poly-L-ornithine (Sigma-Aldrich P3655), and 1 μg/ml laminin (Sigma-Aldrich L2020). Cells were grown in DMEM/F12+GlutaMAX medium (ThermoFisher 31331093) supplemented with 0.05X B27 (ThermoFisher 17504044), 1X N2 (ThermoFisher 17502001), 10 ng/ml bFGF (fisher scientific CTP0261), 10 ng/ml EGF (PeproTech AF-100-15) and 10 U/ml penicillin/streptomycin (ThermoFisher 15140122). Medium was exchanged 50% daily and cells maintained in 5% CO2 at 37ºC, passaging once 100% confluent and seeding at a density of 5x104 cells/cm2. Neural differentiation was induced by growth factor withdrawal the day after plating with media B27 concentration increased to 0.5X. Media was exchanged 50% every second day up until D15, after which media was supplemented with 0.4 ug/ml laminin and exchanged 50% every three days. </p> <p> </p> <p><em>RNA extraction and sequencing</em></p> <p>Cells were lysed in TRIzol reagent (ThermoFischer 15596026) before separating with chloroform and mixing the aqueous phase with isopropanol as per manufacturer directions. RNA was then isolated from the isopropanol/chloroform solution using the ReliaPrep RNA Cell Miniprep kit (Promega Z6010). Libraries were prepared with Illumina Truseq Stranded total RNA RiboZero GOLD kit and sequenced on the NovaSeq6000 platform with a 2x151 setup using NovaSeqXp workflow in S4 mode flowcell.</p> <p> </p> <p><em>Data generation</em></p> <p>Raw reads were processed using cutadapt v3.2 to trim adaptor sequences and low-quality base pairs and discard short reads (options: -m 20 -e 0.1 -q 20 -O 1). The GRCh37 genome assembly was used for all alignment, annotation, and downstream analysis steps. Trimmed read weres alignment to the GRCh37 genome assembly using TopHat v2.0.9 tophat_fusion (with Bowtie v1.1.2 and Samtools v0.1.19) with –fusion-min-dist 200. BAM files have been anonymised by removal of potentially identifiable genetic variant information using BAMboozle v0.5.0 (Ziegenhain & Sandberg, 2021) with default settings. This BAM files and corresponding index (.bai) files are provided here with naming convention "<em>label.</em>bam" Information on sample labels and corresponding conditions is provided in the file 'metadata.txt'.</p> <p><br> </p>
Genetic polymorphisms in COMT and BDNF influence synchronization dynamics of human neuronal oscillations
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Synergistic label-free fluorescence imaging and miRNA studies reveal dynamic human neuron-glial metabolic interactions following injury
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Data from: Gesture encoding in human left precentral gyrus neuronal ensembles
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Large-scale neural recordings with single neuron resolution using Neuropixels probes in human cortex
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Co-occurring ripple oscillations facilitate neuronal interactions between cortical locations in humans
<h3>Contact:</h3><h3>Ilya Verzhbinsky</h3><h3>ilya@health.ucsd.edu</h3><p> </p><p>This is the processed data used to generate the results in the manuscript:</p><p>Co-occurring ripple oscillations facilitate neuronal interactions between cortical locations in humans. <i>PNAS</i> (2023).</p><p>To analyze this data, please first access the code at the following repository: <a href="https://github.com/iverzh/coripple-prediction"><strong>https://github.com/iverzh/coripple-prediction</strong></a></p><p>All downloaded zip files should be uncompressed and placed in a directory named <i>out/ </i>in the <i>CoRipplePredictionPNAS/</i> folder.</p><p> </p><p> </p><p> </p>
Interspecies chimerism with human embryonic stem cells generates functional human dopamine neurons at low efficiency
<p>Interspecies chimeras offer great potential for regenerative medicine and creation of human disease models. Whether human pluripotent stem cell (hPSC) derived neurons in an interspecies chimera can differentiate into functional neurons and integrate into host neural circuity is not known. Here we show, using Engrailed 1 (En1) as a development niche that human naïve-like ES cells can incorporate into embryonic and adult mouse brains. Human-derived neurons including tyrosine hydroxylase (TH) positive neurons integrate into the mouse brain at low efficiency. These TH-positive neurons have electrophysiologic properties consistent with their human origin. Additionally, these human-derived neurons in the mouse brain accumulate pathologic phosphorylated α-synuclein in response to α-synuclein preformed fibrils. Optimization of human/mouse chimeras could be utilized to study human neuronal differentiation and human brain disorders.</p>
The Cellular and Extra-Cellular Proteomic Signature of Human Dopaminergic Neurons Carrying the LRRK2 G2019S Mutation
<p>The provided data sets were generated during the work described in "The Cellular and Extra-Cellular Proteomic Signature of Human Dopaminergic Neurons Carrying the LRRK2 G2019S Mutation" published in Frontiers in Neuroscience, 2024.</p> <p>They contain the results from our differential expression analysis of our raw DIA Protemic Data as well as a list of input data for GO analyses.</p>
Supplementary materials of Brain Extract of Subacute Traumatic Brain Injury Promotes Neuronal Differentiation of Human Neural Stem Cells Via Autophagy
<p>Figure S1: Characterization of hNSCs. In proliferate medium, the embryo derived hNSCs could form the neurospheres (A). Markers of NSCs (NES, SOX2, SOX1) were detected (B). The SOX2<sup>+</sup> cells accounted for >95% of all cells (C). Bar scale: 60μm;</p> <p>Figure S2: The proliferation of hNSCs in TBI brain extracts with different phases. Differentiated hNSCs could be labeled with EdU in TBI brain extracts with different phases (A). Ratio of EdU<sup>+</sup> cells was decreased with the progression of days post-injury (B). Bar scale: 75μm; ****: P<0.0001;</p> <p>Figure S3: The GO annotations and KEGG pathway analysis of different expressed pro-teins in acute brain extract and subacute brain extract;</p> <p>Table S1: The protein profiles of brain extract in acute phase and subacute phase after TBI.</p>
Confocal images of rDRG neurons overexpressing human GDAP1 and its R120W and H123R variants
<p>Original confocal images for panels in Figure 6 in Sutinen et al. (2022). The images correspond to rDRG primary neurons overexpressing RFP or human GDAP1 (wild-type, R120W, H123R variants). Included is a Zip file with 16 TIFF files, one for each panel. </p>
Key Resource Table associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains a Key Resource table associated with: https://doi.org/10.1101/2024.09.24.614704</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.