Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
215
datasets available to search
ShareScore release 0.9.0
Dataset results
215 results for “IgG”
Image data of co-localization of IgG and HEV ORF2 protein in a case of hepatitis E-associated kidney disease
<p><span>Image data for a co-localization study of IgG with HEV ORF2 protein in a </span><span>de novo immune complex-mediated glomerulonephritis (GN) case in</span><span> a kidney transplant recipient </span><span>with chronic hepatitis E (Leblond and Helmchen, et al. 2024).<span> </span>Immunofluorescence images are provided for 25 glomeruli at low magnification (20x, 0.227 micron/pixel) and for 16 glomeruli at high magnification (100x, 0.0454 micron/pixel). For each example glomeruli the green channel represents IgG antibody staining with FITC, and the magenta channel represent anti-HEV ORF2 staining using Alexa Fluor 546.</span></p> <p><span>Methods: </span></p> <p><span>Mouse monoclonal antibody clone 1E6 against the HEV ORF2 protein was incubated for 1h at a dilution of 1:125 followed by a mix of Alexa Fluor 546-conjugated goat anti-mouse antibody (Invitrogen BV, A11018) and FITC-conjugated Rabbit anti-Human IgG (Gamma chain, Diagnostic Biosystem, F008) for 1hat a dilution of 1:50. Following automated staining, the slides were hand -washed in distilled H<sub>2</sub>O. Tissue was covered with Vectashield® Antifade Mounting Medium with DAPI (VectorLaboratories, H-1200), covered with a coverslip and stored at 4°C until evaluation.</span></p> <p><span>Immunofluorescence images were acquired with an upright fluorescence microscope (AxioImager.Z2 controlled by ZEN Blue software; 89 North Photofluor LM-75 light source, and Axiocam 503 mono camera; Zeiss, Jena, Germany), equipped with the following objectives: 20x (NA 0.5, Plan-NEOFLUAR), 40x (NA 1.4 oil, Plan-APOCHROMAT), and 100x (NA 1.45 oil, Plan-APOCHROMAT) objectives. This setup provides an excellent spatial resolution (nominally about 200 nm lateral resolution in our study; pixel size was 45.4 nm for 100x objective). High resolution images were taken with the 100x objective using the ApoTome.2 module with deconvolution (grid 5 lp/mm; section thickness 0.7 µm). We used Vysis Abbott Chroma filter sets (Blue: excitation (ex) 335-383 nm, emission (em) 420-470; green: ex 481-507 nm; em 521-551 nm; red: ex 534-556 nm, em 574- 606 nm). Co-localization of IgG and HEV ORF2 staining was quantified using Fiji software (Schindelin et al., 2012) and the JACoP ImageJ plug-in. </span></p>
CO2 Net Ecosystem Exchange (NEE) and Ecosystem Respiration (ER) + meteorological parameters in alpine grasslands at Nivolet Plain, Gran Paradiso National Park, 2020 (IGG-CNR-CZO@NIVOLET)
<p>CO2 Net Ecosystem Exchange (NEE) and Ecosystem Respiration (ER) measured at Nivolet Plain, Gran Paradiso National Park, Italy, in a high-altitude Alpine grassland environment (about 2500-2700 m.a.s.l.) using the closed portable flux chamber method during the 2020 vegetative season (July-October), approximately twice a month. NEE is measured with a transparent chamber, while ER with a dark chamber (transparent chamber shaded with a cloth). Data represent the average values and the corresponding standard deviations obtained from five sites at different altitudes and soil substrates. Each average value is obtained as a mean over a set of 20 point-measurements for each site and each sampling date. Flux data are complemented by measurements of soil temperature and soil volumetric water content, air temperature, air RH, and solar radiance.</p> <p>During the measurement, air is pumped from the chamber to an IR gas analyzer (IRGA) and then injected again in the chamber. The CO2 concentration inside the chamber is measured for about 90 seconds and then the rate of concentration change is linearly interpolated (over 60s) to obtain the flux measurements. A detailed description of the sampling method can be found in Magnani et al. (2020).</p> <p>Instrumentation used: accumulation chambers (height: 31.5 cm; area of the base: 363 cm2), LI-COR LI-840 & LI-850 IR spectrophotometers, stainless-steel collars (inserted into the soil to a depth of about 1 cm), portable meteorological stations (pyranometer LSI Lastem DPA053, thermohygrometer LSI Lastem DMA672.1), pt100 soil temperature sensors, SM150T soil moisture sensor.</p>
CO2 Net Ecosystem Exchange (NEE) and Ecosystem Respiration (ER) + meteorological parameters in alpine grasslands at Nivolet Plain, Gran Paradiso National Park, 2021 (IGG-CNR-CZO@NIVOLET)
<p>CO2 Net Ecosystem Exchange (NEE) and Ecosystem Respiration (ER) measured at Nivolet Plain, Gran Paradiso National Park, Italy, in a high-altitude Alpine grassland environment (about 2500-2700 m.a.s.l.) using the closed portable flux chamber method during the 2021 vegetative season (July-October), approximately twice a month. NEE is measured with a transparent chamber, while ER with a dark chamber (transparent chamber shaded with a cloth). Data represent the average values and the corresponding standard deviations obtained from five sites at different altitudes and soil substrates. Each average value is obtained as a mean over a set of 20 point-measurements for each site and each sampling date. Flux data are complemented by measurements of soil temperature and soil volumetric water content, air temperature, air RH, and solar radiance.</p> <p>During the measurement, air is pumped from the chamber to an IR gas analyzer (IRGA) and then injected again in the chamber. The CO2 concentration inside the chamber is measured for about 90 seconds and then the rate of concentration change is linearly interpolated (over 60s) to obtain the flux measurements. A detailed description of the sampling method can be found in Magnani et al. (2020).</p> <p>Instrumentation used: accumulation chambers (height: 31.5 cm; area of the base: 363 cm2), LI-COR LI-840 & LI-850 IR spectrophotometers, stainless-steel collars (inserted into the soil to a depth of about 1 cm), portable meteorological stations (pyranometer LSI Lastem DPA053, thermohygrometer LSI Lastem DMA672.1), pt100 soil temperature sensors, SM150T soil moisture sensor.</p>
KCNA2 IgG Autoimmunity In Neuropsychiatric Diseases
<p>One patient of the cohort (#25) received a temporal lobe biopsy to rule out a malignant disease. Histological and immunohistochemical images of diagnostic sections are shown. From these images regions of interest are shown in Figure 2.</p>
Characterization of the anti-spike IgG immune response to COVID-19 vaccines in people with a wide variety of immunodeficiencies
<p>Participants submitted saliva using the OME-505 collection device (OMNIgene Oral, Ottawa, Canada) every two weeks from vaccination through six months post-dose 3 to detect breakthrough SARS-CoV-2 infections. Viral RNA was extracted using the NucliSENS easyMag automated extraction system from 200ul of saliva in stabilizing solution and eluted in a total volume of 50ul. First strand cDNA synthesis was performed from 5ul of eluted RNA using SuperScript IV VILO Master Mix (Thermo Fisher). Positive specimens were then sequenced. Multiplex tiled amplicon libraries were prepared using the Midnight panel and Rapid barcoding kit RBK-004 (Oxford Nanopore technologies) using previously published protocol. Twelve sample pooled libraries were sequenced on a GridION X5 nanopore sequencer using Flongle adapters. After sequencing, raw data were processed using interARTIC to generate consensus sequences and variant calls. SARS-CoV-2<strong> </strong>lineages were determined using these consensus sequences and the NextClade and Pangolin platforms.</p>
Fig. 1 in Presence of IgG antibodies is not a reliable marker of Toxoplasma gondii infection in feral mice
Fig. 1. Frequency distribution of body weight in Toxoplasma gondii B1 PCRnegative (in green) and positive mice (in red). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Diagnostic accuracy of a novel enzyme‑linked immunoassay for the detection of IgG and IgG4 against Strongyloides stercoralis based on the recombinant antigens NIE/SsIR.
<p>Background: The diagnosis of strongyloidiasis is challenging. Serological tests are acknowledged to have high sensitivity, but issues due to cross-reactions with other parasites, native parasite antigen supply and intrinsic test variability do occur. Assays based on recombinant antigens could represent an improvement. The aim of this study was to assess the sensitivity and specificity of two novel immunoglobulin (Ig)G and IgG4 enzyme-linked immunosorbent assays (ELISAs) based on the recombinant antigens NIE/SsIR for the diagnosis of strongyloidiasis.</p> <p>Methods: This was a retrospective diagnostic accuracy study. We included serum samples collected from immigrants from strongyloidiasis endemic areas for whom there was a matched result for Strongyloides stercoralis on agar plate culture and/or PCR assay, or a positive microscopy for S. stercoralis larvae. For the included samples, results were also available from an in-house indirect fluorescent antibody test (IFAT) and a commercial (Bordier ELISA; Bordier Affinity Products SA) ELISA. We excluded: (i) samples with insufficient serum volume; (ii) samples from patients treated with ivermectin in the previous 6 months; and (iii) sera from patients for whom only routine coproparasitology was performed after formol–ether concentration, if negative for S. stercoralis larvae. The performance of the novel assays was assessed against: (i) a primary reference standard, with samples classified as negative/positive on the basis of the results of fecal tests; (ii) a composite reference standard (CRS), which also considered patients to be positive who had concordant positive results for the IFAT and Bordier ELISA or with a single “high titer” positive result for the IFAT or Bordier ELISA. Samples with a single positive test, either for the IFAT or Bordier ELISA, at low titer, were considered to be “indeterminate,” and analyses were carried out with and without their inclusion.</p> <p>Results: When assessed against the primary reference standard, the sensitivities of the IgG and IgG4 ELISAs were 92% (95% confidence interval [CI]: 88–97%) and 81% (95% CI: 74–87%), respectively, and the specificities were 91% (95% CI: 88–95%) and 94% (95% CI: 91–97%), respectively. When tested against the CRS, the IgG ELISA performed best, with 78% sensitivity (95% CI: 72–83%) and 98% specificity (95% CI: 96–100%), when a cut-off of 0.675 was applied and the indeterminate samples were excluded from the analysis.</p> <p>Conclusion: The NIE-SsIR IgG ELISA demonstrated better accuracy than the IgG4 assay and was deemed promising particularly for serosurveys in endemic areas.</p>
Seroprevalence of IgG antibodies against SARS coronavirus 2 in Belgium – a serial prospective cross-sectional nationwide study of residual samples (March – October 2020)
<p>This dataset contains information on seven prospective cross-sectional nationwide residual sera collection rounds. The samples were analyzed for IgG antibodies against S1 proteins of SARS-CoV-2 with a semi-quantitative commercial ELISA (EuroImmun, Luebeck, Germany).</p> <p>We provide a CSV file containing the following variables:</p> <ul> <li><strong>code</strong>: unique sample code</li> <li><strong>age_cat</strong>: age categories by 10-year age bands (0-10, 10-20, ..., 80-90, 90-Inf), the lower limit is included, e.g. 0-10 = [0,10)</li> <li><strong>sex</strong>: sex (f = female, m = male)</li> <li><strong>province</strong>: province of residence (11 categories)</li> <li><strong>region</strong>: region of residence (3 categories: Brussels, Flanders, Walloon)</li> <li><strong>collection_round</strong>: collection round (values 1 to 7)</li> <li><strong>collection_start</strong>: start date of the collection round</li> <li><strong>collection_end</strong>: end date of the collection round</li> <li><strong>igg_orig</strong>: measured IgG OD value as character (note, a semi-quantitative ELISA was used, i.e. this should not be interpreted continiously)</li> <li><strong>igg_cat</strong>: categorized IgG OD values <ul> <li><em>LoD</em>: IgG OD < 0.15</li> <li><em>negative</em>: 0.15 ≤ IgG OD < 0.8</li> <li><em>borderline</em>: 0.8 ≤ IgG OD < 1.1</li> <li><em>positive</em>: 1.1 ≤ IgG OD</li> </ul> </li> </ul> <p>Please see publication mentioned underneath for more details (<a href="https://doi.org/10.1101/2020.06.08.20125179">https://doi.org/10.1101/2020.06.08.20125179</a>).</p> <p><strong>Funding:</strong> This work received funding from the European Union's Horizon 2020 research and innovation program - project EpiPose (No 101003688), the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement 682540 TransMID), the Flemish Research Fund (FWO 1150017N) and from The Antwerp University Fund; which is a community of donors who contribute to research and education with their personal commitment through a donation, gift, bequest or through academic chairs. The funders had no role in study design, data collection, data analysis, data interpretation, writing or submitting of the report. The corresponding author had full access to all the data in the study and had final responsibility for the decision to submit for publication.</p>
Characterization of the anti-spike IgG immune response to COVID-19 vaccines in people with a wide variety of immunodeficiencies
<p>Research on COVID-19 vaccination in immune-deficient/disordered people (IDP) has primarily focused on cancer and organ transplantation populations. In a prospective cohort of 195 IDP and 35 healthy volunteers, anti-spike IgG was detected in 88% of IDP post-dose 2, increasing to 93% by six months post-dose 3. Despite high seroconversion, median IgG levels for IDP never surpassed 1/3 that of healthy volunteers. IgG binding to Omicron BA.1 was lower than all other variants. Angiotensin-converting enzyme 2 pseudo-neutralization (% inhibition) was only modestly correlated with anti-spike IgG concentration. IgG levels were not significantly altered by participants' use of different mRNA-based vaccines, immunomodulating treatments, and prior SARS-CoV-2 infections. While our data show that three doses of COVID-19 vaccinations induce anti-spike IgG in most IDP, additional doses are needed to achieve the levels of protection in healthy volunteers. Due to the strikingly reduced IgG response to Omicron BA.1, the efficacy of additional vaccinations, including bivalent vaccines, should be studied in this population.</p>
Characterization of the anti-spike IgG immune response to COVID-19 vaccines in people with a wide variety of immunodeficiencies
Open the record for dataset details and reuse information.
Data for: Single versus serial dilution SARS-CoV-2 IgG
<p>Reliable and scalable seroepidemiology methods are needed to estimate SARS-CoV-2 incidence and monitor the dynamics of population-level immunity as the pandemic evolves. We aimed to evaluate the reliability of SARS-CoV-2 normalized ELISA optical density (nOD) at a single dilution compared to titers derived from serial dilutions. We conducted serial serosurveys within a community-based cohort in Salvador, Brazil. Anti-S IgG ELISA (Euroimmun AG) was performed with five serial 3-fold dilutions of paired sera from 54 participants. Changes in nOD reliably predicted increases and decreases in titers (98.1% agreement, <span>κ </span>= 95.8%). Fitting the relationship between nOD and interpolated titers to a log-log curve yields highly accurate predictions of titers (r<sup>2</sup> = 0.995) and changes in titers (r<sup>2</sup> = 0.975), using only one to two dilutions. This approach can significantly reduce the time, labor and resources needed for large-scale serosurveys to ascertain population-level changes in exposure and immunity.</p>
Normalised absorbance ratios of IgG for myxoma and rabbit hemorrhagic disease viruses: Longitudinal dataset wild European rabbits
<p>This study addresses the long-term dynamics of humoral immunity to two viral pathogens of the European rabbit, an endangered keystone species of major ecological relevance. Such studies are particularly challenging in free-ranging species, and a combination of longitudinal capture-mark-recapture and semi-quantitative serology was used to address this question. Over 600 normalised absorbance ratios of indirect ELISA, obtained from 505 individual rabbits in 7 populations over five years were analysed using linear mixed models. The results support a lifelong acquired humoral immunity to myxoma virus and rabbit hemorrhagic disease virus upon natural infection and suggest the presence of maternal immunity to the latter in wild juvenile rabbits. These results contribute to understanding the epidemiology of two viral diseases threatening this keystone species and assist in developing conservation programs.</p>
Study of Subcutaneous Immune Globulin in Patients Requiring IgG Replacement Therapy (Japan Study)
ClinicalTrials.gov study NCT01199705. IPD Sharing: Not stated. Countries: 1. Publications: 2.
An Open Label Study of IgG Fc Glycan Composition in Human Immunity
ClinicalTrials.gov study NCT01967238. IPD Sharing: NO. Countries: 1. Publications: 1.
Study of Subcutaneous Immune Globulin in Patients Requiring IgG Replacement Therapy
ClinicalTrials.gov study NCT00542997. IPD Sharing: Not stated. Countries: 9. Publications: 2.
Study of Subcutaneous Immunoglobulin in Patients With PID Requiring IgG Replacement Therapy
ClinicalTrials.gov study NCT00419341. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Study of Subcutaneous Immune Globulin in Patients Requiring IgG Replacement Therapy (EU Extension Study)
ClinicalTrials.gov study NCT00751621. IPD Sharing: Not stated. Countries: 8. Publications: 1.
Data for: Single versus serial dilution SARS-CoV-2 IgG
Open the record for dataset details and reuse information.
RNAseq data relating to manuscript: Prior Fc Receptor activation primes macrophages for increased sensitivity to IgG via long term and short term mechanisms.
Open the record for dataset details and reuse information.
Normalised absorbance ratios of IgG for myxoma and rabbit hemorrhagic disease viruses: Longitudinal dataset wild European rabbits
Open the record for dataset details and reuse information.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.