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ShareScore release 0.9.0
Dataset results
25 results for “Jurkat cell line”
KSR inhibitor APS-2-79 sensitivity test in JURKAT and ALL-SIL T-cell acute lymphoblastic leukemia cell lines measured by Cell Counting Kit 8
<p>APS-2-79 compound was purchased from MedChemExpress (Monmoutyh Junction, NJ, USA). The 20 mg/ml stock solution was prepared in DMSO. To calculate the IC50, JURKAT and ALL-SIL cells were cultured for 72h with a range of APS-2-79 concentrations (5-15 µM) added as equal volumes. Cells treated with 0.5% DMSO (vehicle) were used as negative control. Cells treated with 10% DMSO were used as positive control. The viability of cells was measured using Cell Counting Kit 8 (Sigma Aldrich) and GloMax Microplate Reader system (Promega) with 450 nm wavelength and 600 nm as reference wavelength. The relevant reads are made from following wells: 2A-2D (15 µM APS-2-79), 3A-3D (12.5 µM APS-2-79), 4A-4D (10 µM APS-2-79), 5A-5D (7.5 µM APS-2-79), 6A-6D (5 µM APS-2-79), 7A-7D (vehicle), 8A-8D (positive control).</p>
Genomic variant data for the Jurkat cell line
<p>Variant calling data from whole-genome sequencing of the Jurkat cell line. The data set includes output files from four variant calling tools (jurkat_raw_variant_caller_output.tar.gz), final variant calls after filtering and merging the calls from the separate tools (jurkat_final_variant_calls.tar.gz), and files with variant effect information (jurkat_variant_effects.tar.gz).</p>
Functional anaysis of miR-143-3p/KSR2 interaction and oncogenic function in JURKAT and ALL-SIL T-cell acute lymphoblastic leukemia cell lines
<p>1. FCS files from GFP competition assay performed in ALL-SIL and JURKAT cell lines upon transduction with hsa-mir-143 expression vector (pCDH miR-143-3p) or empty vector (pCDH EV) as control.</p><p>2. Uncropped chemiluminescent immunoblot in JURKAT and ALL-SIL cell lines transduced with hsa-mir-143 expression vector (pCDH miR-143-3p) or empty vector (pCDH EV) as control. Upper band is KSR2 protein (~100 kDa) and lower band is loading control GAPDH protein (~37 kDa). Order of samples on the membrane: JURKAT pCDH miR-143-3p replicate 1, pCDH EV replicate 1, pCDH EV replicate 2, pCDH miR-143-3p replicate 2, pCDH EV replicate 3, pCDH miR-143-3p replicate 3, ALL-SIL pCDH miR-143-3p replicate 1, pCDH miR-143-3p replicate 2, pCDH EV replicate 1, pCDH miR-143-3p replicate 3, pCDH EV replicate 2, pCDH EV replicate 3.</p><p>3. RT-qPCR amplification data for relative quantification of <i>KSR2 </i>expression in reference to <i>ACTB </i>and <i>GAPDH </i>in JURKAT and ALL-SIL cell lines expressing deadCas9-KRAB system for transcriptional repression, upon transduction with sgRNA targeting <i>KSR2 </i>transcription start site vector (<i>KSR2 </i>sgRNA1 and <i>KSR2 </i>sgRNA2) or non-targeting sgRNA vector (NT) as control.</p><p>4. FCS files from GFP competition assay performed in ALL-SIL and JURKAT cell lines expressing deadCas9-KRAB system for transcriptional repression, upon transduction with sgRNA targeting <i>KSR2 </i>transcription start site vector (<i>KSR2 </i>sgRNA1 and <i>KSR2 </i>sgRNA2) or non-targeting sgRNA vector (NT) as control.</p>
Analysis for expression of genes in human leukemic cell line Jurkat treated with Me6TREN or Phosphate Buffered Saline (PBS) control
GEO Series GSE51974. Homo sapiens. 2 samples. Type: Expression profiling by array.
mRNA expression levels in Jurkat cell lines treated with or without IFN-alpha and with shaking or static
GEO Series GSE135370. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome analysis of human T-cell Jurkat cell line in resting cells (t0) and at 8h, 24h and 48h post-activation using Raji B-cells conjugated with superantigen (SEE)
GEO Series GSE94970. Homo sapiens. 12 samples. Type: Expression profiling by array.
Gene expression of native Jurkat human leukemia cell line following 8h Brd4 inhibition by MMQO
GEO Series GSE100361. Homo sapiens. 4 samples. Type: Expression profiling by array.
PRMT7 knockdown in Jurkat-Cas9 and Molt-4 T-ALL cell line
GEO Series GSE186238. Homo sapiens. 21 samples. Type: Expression profiling by high throughput sequencing.
Gene expression of native Jurkat human leukemia cell line following 3h BET inhibition by MMQO or 3h HDAC inhibition by TSA
GEO Series GSE100362. Homo sapiens. 8 samples. Type: Expression profiling by array.
Glycine Chloramine directed gene expression changes in Jurkat T cell lines
GEO Series GSE199235. Homo sapiens. 26 samples. Type: Expression profiling by high throughput sequencing.
Genomic instability of Jurkat clone E6-1 T-cell lines affect gene expression and function: implications for T-cell researchers
GEO Series GSE263543. Homo sapiens. 4 samples. Type: Genome variation profiling by SNP array; Genome variation profiling by genome tiling array.
Gene expression in Jurkat T cell lines
GEO Series GSE122499. Homo sapiens. 9 samples. Type: Expression profiling by array.
SIX6 knockdown in Jurkat-Cas9 T-ALL cell line
GEO Series GSE148658. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
ATAC-seq of isogenicHIV-1 infected Jurkat T cell lines
GEO Series GSE229927. Homo sapiens. 61 samples. Type: Other.
Genome reorganisation and transcriptional changes during activation of the human T-cell line Jurkat
GEO Series GSE94972. Homo sapiens. 16 samples. Type: Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing.
Gene expression profile of the human T-ALL cell line JURKAT after TYK2 and STAT1 knockdown
GEO Series GSE44652. Homo sapiens. 12 samples. Type: Expression profiling by array.
miRNA sequencing using Jurkat and JinB8 T cell line (CD47-deficient) and their Extracellular vesicles (EVs)
GEO Series GSE168187. Homo sapiens. 12 samples. Type: Non-coding RNA profiling by high throughput sequencing.
ATAC-seq analysis of wild-type Jurkat, SV engineered Jurkat, DND-41 and LOUCY leukemia cell lines
GEO Series GSE199691. Homo sapiens. 7 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
ATAC-seq analysis of Jurkat leukemia cell lines
GEO Series GSE132209. Homo sapiens. 1 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
RNA-Sequencing experiment for effects of PKF115-584 treatment on four T-ALL cell lines (RPMI8402, HPB-ALL, Jurkat, CCRF-CEM).
GEO Series GSE69157. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
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