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46 results for “Juvenile Hormone”
Fig. 1 in Effects of nucleopolyhedrovirus infection on the development of Helicoverpa armigera (Lepidoptera: Noctuidae) and expression of its 20-hydroxyecdysone- and juvenile hormone-related genes
Fig. 1. Effect of HaSNPV infection on the larval body weight of Helicoverpa armigera. (A) ♦, ■, ▲, ×, —, and ● indicate larvae infected with HaSNPV at the concentrations 0, 105, 106, 107, 108, and 109 PIB/mL, respectively. (B) a, b, c, d, e, and f show H. armigera larvae on the 5th day afer infection with HaSNPV at the concentrations 0, 105, 106, 107, 108, and 109 PIB/mL, respectively.
Fig. 3 in Effects of nucleopolyhedrovirus infection on the development of Helicoverpa armigera (Lepidoptera: Noctuidae) and expression of its 20-hydroxyecdysone- and juvenile hormone-related genes
Fig. 3. Expression analysis by qRT-PCR of the JH related genes MET, JHEH, HSP90, and JHi in Helicoverpa armigera larvae afer HaSNPV infection at 0, 24, 48, 72, 96, and 120 h. The blank bars represent the larvae infected with sterile water (CK). The black bars represent the larvae infected with NPV at the concentration of 107 PIB/mL (NPV infection). The data represent the mean ± SD of 3 biological replicates. Statistically significant differences from gene expression are denoted by * (0.01 <P £ 0.05) and ** (P £ 0.01) as determined by the pairwise Student's t-test analysis in SPSS 17.0 sofware.
Fig. 2 in Effects of nucleopolyhedrovirus infection on the development of Helicoverpa armigera (Lepidoptera: Noctuidae) and expression of its 20-hydroxyecdysone- and juvenile hormone-related genes
Fig. 2. Expression analysis by qRT-PCR of the 20E related genes ECR, USP, E75, BR, HR3, and NFT2 in Helicoverpa armigera larvae afer HaSNPV infection at 0, 24, 48, 72, 96, and 120 h. The blank bars represent the larvae infected with sterile water (CK). The black bars represent the larvae infected with NPV at the concentration of 107 PIB/mL (NPV infection). The data represent the mean ± SD of 3 biological replicates. Statistically significant differences from gene expression are denoted by * (0.01 <P £ 0.05) and ** (P £ 0.01) as determined by the pairwise Student's t-test analysis in SPSS 17.0 sofware.
Fig. 6 in Identification and characterization of three juvenile hormone genes from Bactrocera dorsalis (Diptera: Tephritidae)
Fig. 6. Effects of starvation on the expression of BdJHEH2, BdJHEH3, and BdJH- DK in Bactrocera dorsalis. The 2-d-old 3rd instars were fed or starved for 24 and 48 h before collection. The re-fed larvae were initially starved for 24 h, then refed for an additional 24 h prior to collection. F24: feeding 24 h; F48: feeding 48 h; S24: starvation 24 h; S48: starvation 48 h; RF: re-fed. Different letters indicate significant differences based on 1-way ANOVA followed by an LSD test (P <0.05).
Fig. 3 in Identification and characterization of three juvenile hormone genes from Bactrocera dorsalis (Diptera: Tephritidae)
Fig. 3. Relative expression levels of BdJHEH2, BdJHEH3, and BdJHDK in developmental stages of Bactrocera dorsalis. Expression levels at 19 time points in (A) 3rd instar larvae and pupae and (B) adults were detected by qPCR. 3L1: 1-d-old 3rd instar larvae; P1: 1-d-old pupae. Females or males were collected for qPCR analysis at 1, 4, 7, and 10 d afer eclosion. Different letters indicate significant differences among females or males based on 1-way ANOVA followed by an LSD test (P <0.05). Significant differences between the females and males determined with a t-test are indicated by * (P <0.05).
Fig. 4 in Identification and characterization of three juvenile hormone genes from Bactrocera dorsalis (Diptera: Tephritidae)
Fig. 4. Relative expression levels of BdJHEH2, BdJHEH3, and BdJHDK in tissues of Bactrocera dorsalis. Expression levels in the head (HD), thorax (TH), midgut (MG), Malpighian tubules (MT), and fat body (FB) were detected by qPCR. Different letters indicate significant differences among tissues based on 1-way ANOVA followed by an LSD test (P <0.05).
Fig. 2 in Identification and characterization of three juvenile hormone genes from Bactrocera dorsalis (Diptera: Tephritidae)
Fig. 2. Multiple sequence alignment and phylogenetic analysis of JHDK from Bactrocera dorsalis and other insects. (A) Sequence alignment. The sequence of B. dorsalis JHDK is compared to SCP2 from Drosophila melanogaster (Dm), and JHDK from Leptinotarsa decemlineata (Ld), Manduca sexta (Ms), Plutella xylostella (Px), and Spodoptera litura (Sl). The α-helices (H1-8) and EF hands are indicated above the alignment according to results from B. mori (Li et al. 2005).Three predicted GTP-binding motifs (Σ1–3) are labeled with an arrow. (B) Phylogenetic analysis of JHDK homologs. The tree was generated with MEGA 5 using the neighbor-joining method. Nodes with>50% bootstrap values (1,000 replicates) are indicated on branches. GenBank accession numbers of all sequences are listed in the tree.
Fig. 1 in Identification and characterization of three juvenile hormone genes from Bactrocera dorsalis (Diptera: Tephritidae)
Fig. 1. Multiple sequence alignment and phylogenetic analysis of JHEHs from Bactrocera dorsalis and other insects. (A) Sequence alignment. The sequences of B. dorsalis JHEHs are compared with JHEH from Drosophila melanogaster (Dm), Bombyx mori (Bm), Manduca sexta (Ms), and Apis mellifera (Am). The catalytic triad (Asp232, Glu409, and His436), 2 tyrosine residues (Tyr 304 and Tyr380), and HGXP motif are labeled with asterisks. The HGXP motif is underlined. (B) Phylogenetic analysis of JHEH homologs. The tree was generated with MEGA 5 using the neighbor-joining method. Nodes with>50% bootstrap values (1,000 replicates) are indicated on branches. GenBank accession numbers of all sequences are listed in the tree.
Juvenile hormone regulates the photoperiodic plasticity of elytra coloration in a ladybird Harmonia axyridis
<p><span>Many animals, including insects, exhibit plasticity of body color in response to environmental changes</span><span>. Varied expression of c</span><span>arotenoids, major cuticle pigments, significantly contributes to body color flexibility. However, the molecular mechanisms by which environmental cues regulate carotenoid expression remain largely unknown. In this study, we used the ladybird <em>Harmonia axyridis</em> as a model to investigate the photoperiodic-responsive plasticity of elytra coloration and its endocrine regulation. It was found that <em>H. axyridis</em> females under long-day conditions develop elytra that are much redder than those under short-day conditions, resulting from the differential accumulation of carotenoids. Exogenous hormone application and RNAi-mediated gene knockdown indicate that carotenoid deposition was directed through the juvenile hormone (JH) receptor-mediated canonical pathway. Moreover, we characterized an SR-BI/CD36 (SCRB) gene <em>SCRB10</em> as the carotenoid transporter responding to JH signaling and regulating the elytra coloration plasticity. Taken together, we propose that JH signaling transcriptionally regulates the carotenoid transporter gene for the photoperiodic coloration plasticity of elytra in the beetles, which reveals a novel role of the endocrine system in the regulation of carotenoid-associated animal body coloration under environmental stimuli.</span></p>
Improving the mating competitiveness of male Anastrepha ludens (Diptera: Tephritidae) fruit flies by adding two juvenile hormone analogues
<p>Improving the mating competitiveness and survival of sterile males are direct means to increasing the effectiveness of the Sterile Insect Technique (SIT). Some insecticide growth regulators, such as the juvenile hormone analogue (JHA) Methoprene, have been used to improve the mating competitiveness of male tephritid flies by reducing their sexual maturation period. However, a decrease in the period of sexual maturation induces a reduction in survival. Here, we compared the effects of methoprene and Pyriproxyfen (PPF), another JHA, in <em>Anastrepha</em> <em>ludens</em> males. Pyriproxyfen is an insect growth regulator that exhibits higher disruption on insects’ methamorphosis than methoprene or than natural JH. . Both compounds were administered at two doses (0.05 and 0.1%) via the male diet immediately after emergence. Our results show that both Pyriproxyfen and methoprene reduced male sexual maturation. However, PPF-treated males exhibited a shorter maturation period and obtained more matings at a given age than methoprene-treated males . No significant differences were observed between the two PPF doses tested (0.05 and 0.10 %). Male survival was equally accelerated by the two compounds. Our results demonstrate that PPF can be used as a tool to improve the mating performance of sterile males.</p>
Growth Hormone in Children With Juvenile Rheumatoid Arthritis (JRA) and With Crohn's Disease
ClinicalTrials.gov study NCT00511329. IPD Sharing: Not stated. Countries: 1. Publications: 5.
Juvenile hormone regulates the maturation of sexually dimorphic naïve ethanol olfactory preference in Drosophila melanogaster
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Data from: Ame-miR-2161 affects the survival and development of honeybee larvae through the juvenile hormone acid methyltransferase gene
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Juvenile hormone regulates the photoperiodic plasticity of elytra coloration in a ladybird Harmonia axyridis
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Histone acetyltransferase p300/CBP regulates reproductive diapause via the juvenile hormone pathway in the cabbage beetle, Colaphellus bowringi
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Data from: Polybrominated diphenyl ether (DE-71) exposure skews phenotypic sex ratio, and alters steroid hormone levels and steroidogenic enzyme activities in juvenile Silurana tropicalis
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The UPLC data of juvenile hormones of various heteropteran species
<p><span>Juvenile hormone (JH) plays important roles in almost every aspect of insect development and reproduction. JHs are a group of acyclic sesquiterpenoids, and their farnesol backbone has been chemically modified to generate a homologous series of hormones in some insect lineages. JH III (epoxidised methyl farnesoate) is the most common JH in insects, but Lepidoptera (butterflies and moths) and 'higher' Diptera (suborder: Brachycera; flies) have developed their own unique JH. Although JH was first proposed in the hemipteran suborder Heteroptera (true bugs), the chemical identity of the heteropteran JH was only recently determined. Furthermore, recent studies revealed the presence of a novel JH, JH III skipped bisepoxide (JHSB<sub>3</sub>), in some heteropterans, but its taxonomic distribution remains largely unknown. In the present study, we investigated JHSB<sub>3</sub> production in 31 heteropteran species, covering almost all heteropteran lineages, through ultra-performance liquid chromatography coupled with tandem mass spectrometry. We found that all of the focal species produced JHSB<sub>3</sub>, indicating that JHSB<sub>3</sub> is wide-spread in heteropteran bugs and the evolutionary occurrence of JHSB<sub>3</sub> ascends to the common ancestor of Heteroptera.</span></p>
X-ray diffraction images of juvenile hormone diol kinase from the silk worm Bombyx mori
<p>X-ray diffraction images of juvenile hormone diol kinase (JHDK) from the silk worm Bombyx mori</p>
Efficacy and Safety of Growth Hormone Treatment in Juvenile Idiopathic Arthritis
ClinicalTrials.gov study NCT00420251. IPD Sharing: Not stated. Countries: 1. Publications: 2.
The UPLC data of juvenile hormones of various heteropteran species
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