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8 results for “LSFM”
2D LSFM timelapse of cardiomyocyte calcium dynamics
<p>Uploaded zip-folder contains the following files:<br> 1. A representative raw dataset of a 2D LSFM ventricular cardiomyocyte undergoing stimulated calcium transients and calcium sparks (frame_0000.tif -frame_17999.tif)<br> 2. The recorded pacing signal time trace (waveform_test.xslx)<br> 3. Image corresponding to the time-averaged background (AVG_19_35_39_LowNA rolling shutter.tif)<br> 4. Pre-processed nuclear mask matrix (NuclearMask.mat), CMO-channel average (CMO_Average.mat), and CMO channel maximum intensity projection (CMO_MIP). <br> 5. Split and co-registered data for each spectral channel (CMO_frame_00001.tif-CMO_frame_18000.tif, FLUO4_frame_00001.tif -FLUO4_frame_18000.tif).<br> <br> Compressed file size: 14.9 GB<br> Uncompressed file size: 42.8 GB. <br> <br> Related to the following manuscript: <br> Liuba Dvinskikh, Hugh Sparks, Ken MacLeod and Chris Dunsby " <em>High-speed 2D light-sheet fluorescence microscopy enables quantification of spatially varying calcium dynamics in ventricular cardiomyocytes</em>" (2023), <em>In review</em> with Frontiers in Physiology, Cardiac Electrophysiology. </p>
HRAS_GFP zebrafish Embryo z-stack and 3D reconstruction visualized through LSFM
<p>A 2dpf zebrafish larvae is imaged through a custom developed LSFM setup developed at ICFO, at the Super-resolution Light microscopy and Nanoscopy (SLN) facility, with a resolution of 1 um, and with a double illumnation scheme.</p> <p>Pixel size is 0.43 um. Voxel depth is 2 um.</p> <p>The transgenic line is expressing HRAS_GFP labeling.</p> <p>The z-stack and corresponding 3D reconstruction are showed.</p> <p> </p> <p> </p>
Quantitative 3D OPT and LSFM datasets of pancreata from mice with streptozotocin-induced diabetes: Dataset 1
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Quantitative 3D OPT and LSFM datasets of pancreata from mice with streptozotocin-induced diabetes: Sample data sets
<p><span>Mouse models for streptozotocin (STZ) induced diabetes probably represent the most widely used systems for preclinical diabetes research, owing to the compound's toxic effect on pancreatic ß-cells. However, a comprehensive view of pancreatic β-cell mass distribution subject to STZ administration is lacking. Previous assessments have largely relied on the extrapolation of stereological sections, which provide limited 3D-spatial and quantitative information. This data descriptor presents multiple ex vivo tomographic optical image data sets of the full β-cell mass distribution in mice subject to single high and multiple low doses of STZ administration, and in glycaemia recovered mice. The data further include information about structural features, such as individual islet β-cell volumes, spatial coordinates, and shape as well as signal intensities for both insulin and GLUT2. Together, they provide the most comprehensive anatomical record of the effects of STZ administration on the islet of Langerhans in mice. As such, this data descriptor may serve as reference material to facilitate the planning, use and (re)interpretation of this widely used disease model.</span></p>
Octopus vulgaris, Sepia officinalis, Loligo vulgaris and Illex coindetii early life phases Light Sheet Fluerescence Microscopy (LSFM) 3D scans.
<p>Acronyms: OV: <em>Octopus vulgaris</em>, SO: <em>Sepia officinalis</em>, LV: <em>Loligo vulgaris</em>, IC: <em>Illex coindetii</em>, DPH: Days Post-Hatching.</p> <p>Two detection objectives were used, depending on sample size, a 4x/0.28 NA Olympus XLFLUOR4x/340 objective (0, 5, 10, 19 DPH <em>Octopus vulgaris</em> individuals,<em> Loligo vulgaris</em> and<em> Illex coindetii</em>) and a Nikon 10x/0.5 NA CFI Plan Apochromat 10xC Glyc (Rest of the samples). For illumination, two 4x/0.95 NA Nikon CFI Plan Apo Lambda 4x were used when using the 10x detection objective and two 4x/0.13 NA Nikon Plan Fluor illumination objectives were used when using the 4x detection objective. </p> <p>Microscope: MuVi SPIM (Luxendo), LCS SPIM (Luxendo, only <em>Sepia officinalis</em> and 60 DPH <em>Octopus vulgaris</em> individuals).</p> <p>All the data has been scaled in order to reduce file sizes. Full size stacks can be requested to dgvilar@gmail.com.</p>
Quantitative 3D OPT and LSFM datasets of pancreata from mice with streptozotocin-induced diabetes: Sample data sets
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Quantitative 3D OPT and LSFM datasets of pancreata from mice with streptozotocin-induced diabetes: Dataset 2 & 3
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LSFM-image z-stack of an optically cleared porcine adipose tissue sample
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The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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