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Figure 4 in Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential

Figure 4. Absorption spectrum of AgNPs synthesized by Lactobacillus bulgaricus having clear peak at 410 nm.

Figure 2 in Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential

Figure 2. Lactobacillus bulgaricus synthesis of Ag-NPs: (a) AgNO3 (control); (b) Reaction mixture before synthesis; (c) Reaction mixture after synthesis.

Figure 1 in Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential

Figure 1. Morphological and microscopic properties of Lactobacillus bulgaricus. (a) Lactobacillus stained with gram stain under light microscope (X100); (b) Lactobacillus on MRS agar medium, 37 °C, 48 h.

Figure 7 in Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential

Figure 7. Antibacterial effect of Ag-NPs and antibiotics against (a) Staphylococcus epidermis (b) Salmonella (c) Staphylococcus aureus.

Figure 5 in Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential

Figure 5. SEM of silver nanoparticles synthesized by Lactobacillus bulgaricus. The shape of AgNPs was spherical and size between (30- 100 nm), magnification (16053×, 17876×, 15568×, and 9742 ×), the voltage (5 kV) and spot size 4 for (a), (b), (c) and (d) respectively.

opencc-by-4.0Dec 2017View details →

Figure 2 in Peptidoglycan from Immunobiotic Lactobacillus rhamnosus Improves Resistance of Infant Mice to Respiratory Syncytial Viral Infection and Secondary Pneumococcal Pneumonia

Figure 2. – Photography of a juvenile lemon shark identified as Negaprion acutidens with an estimated total length of 70 cm (Photo MI).

opencc-by-4.0Dec 2017View details →

Figure 1 in Peptidoglycan from Immunobiotic Lactobacillus rhamnosus Improves Resistance of Infant Mice to Respiratory Syncytial Viral Infection and Secondary Pneumococcal Pneumonia

Figure 1. – The Chesterfield islands (A) lie in the Coral Sea with New Caledonia (NC) to the east and Australia (AUS) to the west. The atoll structure of the Chesterfield (B) includes a V shaped barrier reef in the South (C) where the juvenile lemon sharks were observed (arrow).

opencc-by-4.0Jun 2021View details →

The Influence of Lactobacillus Acidophilus on MUC1, GAL-3, IL-1β and IL-17 Gene Expression in BALB/c Mice Stomach

Gene expression of MUC-1, GAL-3, IL-1 and IL-17 after treatment with low, moderate and high doses of Lactobacillus acidophilus ATCC 4356   in mice

dryad36/100

The cost and benefit of quorum sensing controlled bacteriocin production in Lactobacillus plantarum

Bacteria eliminate competitors via 'chemical warfare' with bacteriocins. Some species appear to adjust bacteriocin production conditionally in response to the social environment. We tested whether variation in the cost and benefit of producing bacteriocins could explain such conditional behaviour, in the bacteria Lactobacillus plantarum. We found that: (1) bacterial bacteriocin production could be upregulated by either the addition of a synthetic autoinducer peptide (PLNC8IF; signalling molecule), or by a plasmid which constitutively encodes for the production of this peptide; (2) bacteriocin production is costly, leading to reduced growth when grown in poor and, to a lesser extent, in rich media; (3) bacteriocin production provides a fitness advantage, when grown in competition with sensitive strains; (4) the fitness benefits provided by bacteriocin production is greater at higher cell densities. These results show how the costs and benefits of upregulating bacteriocin production can depend upon abiotic and biotic conditions.

opencc-zeroOct 2019View details →

opencc-by-4.0Apr 2024View details →

Evaluation of the immunostimulant effect of microvesicles of Lactobacillus acidophilus isolated from wild Rattus norvegicus.

Lactic acid bacteria are components of the microbiota of the gastrointestinal tract in both humans and animals and are widely used as probiotics. The bacterial strain Lactobacillus sp. is the most related to probiotic activity and released membrane microvesicles (MVs). Some of their primary functions are to carry and transmit antigens to the host tissues and modulate the host defense responses. In the present study, Lactobacillus acidophilus was isolated from the ileum of free-living Rattus norvegicus to evaluate the immunostimulant effect of their MVs in two biological models. MVs from L. acidophilus were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electron microscopy, and nanoparticle tracking analysis. The immunostimulant effect of MVs from L. acidophilus on ovine abomasal explants, previously stimulated with 30 µg/1h of MVs and subsequently challenged with third-stage larvae (L3) of Haemonchus contortus, was probed, demonstrating a decrease in the percentage of larvae association, and favoring the migration of inflammatory cells to the infection site. In contrast, cells line of the RAW 264.7 were stimulated by 10 µg/3h of MVs from L. acidophilus to evaluate their activation through the expression of transcripts coding for IL-1β and TNF-α. In both cases, the immunostimulatory effects of MVs from L. acidophilus isolated from free-living R. norvegicus were verified, demonstrating that these structures are capable of immunostimulated the evaluated biological models and could carried important antigen that can be considerate in a formulation to promote the intestinal health in livestock production.  The present study is the first report of MVs secreted by L. acidophilus isolated from free-living R. norvegicus caring that this rodent is resistant to many bacterial pathogens that normally affect the gastrointestinal tract of other mammals. In the atachment file we present the data obtained of the porcentage of asocciation and porcentage of mortality of L3 stage larvae from H. contortus in abomasal tissue stimulated with microvesicles from L. acidophilus. In the other hand the expression of IL-1β and TNFα.  mRNA from RAW 264.7 cells stimulated with microvesicles from L. acidophilus.

opencc-by-4.0Feb 2022View details →

Dataset related to article "Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses "

This record contains raw datarelated to article "Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses " Lactobacillus helveticus carries many properties such as the ability to survive gastrointestinal transit, modulate the host immune response, accumulate biopeptides in milk, and adhere to the epithelial cells that could contribute to improving host health. In this study, the applicability as functional cultures of four L. helveticus strains isolated from Italian hard cheeses was investigated. A preliminary strain characterization showed that the ability to produce folate was generally low while antioxidant, proteolytic, peptidase, and β-galactosidase activities resulted high, although very variable, between strains. When stimulated moDCs were incubated in the presence of live cells, a dose-dependent release of both the pro-inflammatory cytokine IL-12p70 and the anti-inflammatory cytokine IL-10, was shown for all the four strains. In the presence of cell-free culture supernatants (postbiotics), a dose-dependent, decrease of IL-12p70 and an increase of IL-10 was generally observed. The immunomodulatory effect took place also in Caciotta-like cheese made with strains SIM12 and SIS16 as bifunctional (i.e., immunomodulant and acidifying) starter cultures, thus confirming tests in culture media. Given that the growth of bacteria in the cheese was not necessary (they were killed by pasteurization), the results indicated that some constituents of non-viable bacteria had immunomodulatory properties. This study adds additional evidence for the positive role of L. helveticus on human health and suggests cheese as a suitable food for delivering candidate strains and modulating their anti-inflammatory properties.

Figure 6 in Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential

Figure 6. EDS Analysis of AgNPs synthesized by Lactobacillus bulgaricus.

Figure 3 in Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential

Figure 3. Results of suspension at different AgNO 3 concentrations of Lactobacillus bulgaricus.

opencc-by-4.0Jul 2023View details →

DADA2 formatted Silva SSU taxonomic training data (Silva version 138.1) with emended description of the genus Lactobacillus Beijerinck 1901

These training fasta files are derived from the Silva 138.1 prokaryotic SSU taxonomic training data formatted for DADA2 (from <a href="https://zenodo.org/record/4587955">https://zenodo.org/record/4587955</a>). The species assignment file contains changes in species names according to <a href="https://doi.org/10.1099/ijsem.0.004107">Zheng et al. 2020</a> based on data from <a href="https://github.com/swuyts/lactotax/tree/master">Lactotax</a> (file <a href="https://github.com/swuyts/lactotax/raw/master/data/2023_05_30.xlsx">2023_05_30.xlsx</a>). The script in R for making changes in species names is in the silva.R file. Please cite one or both of the Silva references, the DADA2 paper (reference below), and the Zenodo record for this specific version in your Methods or published source code to record the specific taxonomic database files used in your analysis.

restrictedIPD-UNDECIDEDFeb 2026View details →

Obesity - Inflammation - Metabolic Disease: Effect of Lactobacillus Casei Shirota

ClinicalTrials.gov study NCT01182844. IPD Sharing: Not stated. Countries: 1. Publications: 2.

restrictedIPD-UNDECIDEDFeb 2026View details →

Effect of Lactobacillus Rhamnosus GG (LGG) on Infant Colic

ClinicalTrials.gov study NCT01279265. IPD Sharing: Not stated. Countries: 1. Publications: 2.

closedIPD-NOFeb 2026View details →

28-Day Daily-dose Crossover Study of the Safety and Tolerability of SB-121 (Lactobacillus Reuteri With Sephadex® and Maltose) in Subjects, Ages 15 to 45 Years, Diagnosed With Autistic Disorder

ClinicalTrials.gov study NCT04944901. IPD Sharing: NO. Countries: 1. Publications: 1.