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11 results for “Lasius niger”
Data for "Not dear neighbours in the ant Lasius niger"
<p><strong>This README accompanies the files "Rmarkdown_Cordonnier.pdf", “data_final.txt”, and “Genotypes.xlsx”</strong></p> <ul> <li><strong><em>Rmarkdown_Cordonnier.pdf</em></strong></li> </ul> <p>********************************** CONTENTS ***********************************</p> <p>Rmarkdown for ‘Not dear neighbours: Antennation and jerking, but not aggression, correlate with genetic distance and physical distance in the ant <em>Lasius niger’</em></p> <p> </p> <ul> <li><strong><em>Data_final.txt</em></strong></li> </ul> <p>********************************** CONTENTS ***********************************<br> Dataset associated with the Rmarkdown</p> <p>The data are in table form with TABs as variables field delimiters so they can <br> be readily imported in any statistical package or spreadsheet program. Please, <br> contact me if you need the file formatted otherwise. </p> <p>This file includes a description of the variables.</p> <p>*******************************************************************************<br> Variable names and descriptions</p> <p>date date of the experiment</p> <p>time time of the experiment</p> <p>temp_outdoor outdoor temperature during the experiment</p> <p>temp_indoor indoor temperature during the experiment</p> <p>pair_ID ID of the pair of individuals tested in the experiment</p> <p>target_col ID of the target colony tested in the experiment</p> <p>comp_col ID of the competitor colony tested in the experiment</p> <p>distance geographical distance between the colonies involved in the experiment</p> <p>group allospecific (interactants from different species),</p> <p> nestmate (interactants from the same species, same colony),</p> <p> non-nestmate (interactants from same species, different colonies)</p> <p>neighbour neighbour interactants (adjacent slabs) versus non-neighbour interactants</p> <p>neutral_body_contact number of neutral body contact between the ants during the interaction</p> <p>antennation number of occurrences of antennation during the interaction</p> <p>jerking number of occurrences of jerking during the interaction</p> <p>mandible_gaping number of occurrences of mandible gaping during the interaction</p> <p>brief_biting number of occurrences of brief biting during the interaction</p> <p>gaster_flexing number of occurrences of gaster flexing during the interaction</p> <p>fighting_prolonged_biting number of occurrences of fighting or prolonged biting over the interaction</p> <p>RQG_Col relatedness between pairs of colonies (estimator of Queller & Goodnight)</p> <p> </p> <ul> <li><strong><em>Genotypes.xlsx</em></strong></li> </ul> <p>********************************** CONTENTS ***********************************</p> <p>Genotypes of the workers</p> <p>IDcolony ID of the source colony of the genotyped worker</p> <p>Ant1343 to ant2794 list of the 11 microsatellite markers used in the study</p> <p>The 15 µl PCR reaction volume consisted of 7.5 µl GoTaq® G2 Hot Start Colorless Master Mix (M7433, Promega), 4.5 µl ddH2O, 1.0 µl unlabelled reverse primer (10µM), 1.0 µl labelled forward primer (10µM; labelled HEX, FAM and TET; final concentration of 0.67µM for each primer) and 1 µl DNA (2-10 ng). The PCR consisted of initial denaturation at 94°C (4 min), 33 cycles at 94°C (denaturation, 30 s), 55°C (annealing, 30 s) and 72°C (elongation, 30 s), and a final step at 72°C (1 min). The PCR products were analysed in an ABI PRISM 310 Genetic Analyser (PE Biosystems) after DNA denaturation at 90°C (1 min). Allele sizes were determined using genescan 3.1 software (PE Biosystems).</p> <p> </p> <p>********************************* CONTACT **********************************<br> Please contact me at:</p> <p>Marion Cordonnier<br> e-mail: marion.cordonnier@hotmail.com</p> <p>*******************************************************************************</p>
Figs 7–8 in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)
Figs 7–8. Indirect flight muscles, gaster fat body and ovaries in four young queens of F. rufa: 7 — from left to right: dissected mesosoma; gaster without tergites; gaster without part of the fat body for two alates; 8 — the same anatomy as described in 7 for two single dealate queens; abbreviations in the text. Scales: 1 mm. Рис. 7–8. Крыловые мыШцы непрЯмого действиЯ, жировое тело и Яичники четырёх молодых самок F. rufa: 7 — слева-направо: вскрытаЯ меЗосома; брюШко беЗ тергитов; брюШком с удаленной частью жирового тела у двух крылатых самок, 8 — то же, у двух бескрылых одиночных самок; обоЗначениЯ в тексте. МасШтаб — 1 мм.
Fig. 3 in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)
Fig. 3. Stages of indirect flight muscles degeneration in Formica rufa queens. Lateral view of a dissected mesosoma. Arrows indicate transitions from stages without external signs of necrosis (0) to stages in which hystolysis of muscle fibers occur (Hi1, Hi2) and their replacement by columns of adipocytes (Ad1, Ad2); other abbreviations — in the text. Рис. 3. Стадии дегенерации крыловых мыШц непрЯмого действиЯ у самок F. rufa. ВскрытаЯ меЗосома, сбоку. Стрелками покаЗаны переходы от стадии беЗ проЯвлений некроЗа (0) до гистолиЗа мыШечных волокон (Hi1, Hi2) и их ЗамеЩениЯ колонками адипоцитов (Ad1, Ad2); остальные обоЗначениЯ — в тексте.
Figs 4–6 in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)
Figs 4–6. Indirect flight muscles, gaster fat body and ovaries in three young dealate queens of L. niger: 4–5 — from left to right, a dissected mesosoma from a lateral view, a dorsal view of gaster without tergites, gaster without part of fat body; 6 — from left to right, a dissected mesosoma from a lateral view, ovaries from dorsal and ventral view; abbreviation in the text. Scales: 1 mm. Рис. 4–6. Крыловые мыШцы непрЯмого действиЯ, жировое тело брюШка и Яичники трёх молодых бескрылых самок L. niger: 4– 5 — слева-направо, вскрытаЯ меЗосома, сбоку; вид на брюШко беЗ тергитов, сверху; то же — удалена часть жирового тела; 6 — слева- направо, вскрытаЯ меЗосома, сбоку; Яичники — сверху и сниЗу; обоЗначениЯ в тексте. МасШтаб — 1 мм.
Figs 1–2 in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)
Figs 1–2. Reproductive systems in queen and workers of red wood ants: 1 — polytrophic ovariole, ovaries and oviducts in queen [partly after Schwanwitsch, 1949 and Otto, 1962]; 2 — stages of ovarian development in workers of F. rufa [after Otto,1958]; J — initial stage of ovarie, E1–E3 and V — growth stages, R1, R2 — stages of resorption, D — stage of degeneration; other — in the text. Рис. 1–2. ПоловаЯ система самки и рабочих у рыжих лесных муравьёв: 1 — политрофическаЯ ЯйцеваЯ трубочка, Яичники и половые пути самки [частично по Schwanwitsch, 1949 и Otto, 1962]; 2 — стадии раЗвитиЯ Яичников у рабочих F. rufa [по Otto,1958]; J — начальнаЯ стадиЯ Яичника, E1–E3 и V — стадии роста Яичников, R1, R2 — стадии реЗобциии, D — стадии дегенерации; прочeе — в тексте
Figs 9–12. A in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)
Figs 9–12. A dissected mesosoma, gaster fat body and ovaries in familial dealate queens of F. rufa at different stages of indirect flight muscle degeneration are shown:9 — at the stage of progressive hystolysis, Hi1; 10 — at the stage of active hystolysis, Hi2; 11 — at the stage of progressive adipogenesis, Ad1; 12 — at the stage of completed adipogenesis, Ad2; abbreviations uin the text. Scale — 1 mm Рис. 9–12. МеЗосома, жировое тело брюШка и Яичники четырех семейных самок F. rufa на раЗных стадиЯх дегенерации крыловой мускулатуры: 9 — на стадии продвинутого гистолиЗа Hi1; 10 — на стадии активного гистолиЗа Hi2; 11 — на стадии продвинутого адипогенеЗа Ad1; 12 — ЗаверШенного адипогенеЗа Ad1. МасШтаб — 1 мм
Fig. 2 in Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)
Fig. 2. Neighbor-joining tree based on 16S rRNA gene sequences showing the relationship between actinomycetes associated with Lasius niger and related type strains. Strains from different nests are marked with pictograms of different colors: Krylatskoye (black), Leninskie gory (gray), Filevsky park (white). Рис. 2. Филогенетическое положение Штаммов актиномицетов, ассоциированных с Lasius niger и блиЗких им генетически типовых Штаммов стрептомицетов, на основании NJ-аналиЗа нуклеотидных последовательностей фрагмента гена 16S рРНК. Штаммы иЗ раЗных гнеЗд отмечены пиктограммами раЗных цветов: Крылатское (черные), Ленинские горы (серые), Филевский парк (белые).
Fig. 1 in Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)
Fig. 1. Nocardia strains in heads of Lasius niger are most related to Nocardia fluminea DSM 44489: cultural features on ISP 2 (left), morphological characteristics studied by SEM (center), the structure of actinomycete communities on ants (right). Рис. 1. Представители рода Nocardia в головном отделе Lasius niger представлены Штаммами, наиболее блиЗкими к Nocardia fluminea DSM 44489: культуральные приЗнаки на среде ISP2 (слева), морфологические приЗнаки, иЗученные с помоЩью сканируюЩей Электронной микроскопии (в центре), структура актиномицетных комплексов (справа).
Fig. 4 in Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)
Fig. 4. Antagonistic activity of actinomycete strains, associated with L. niger: a — heatmap of antagonism to Bacillus thuringiensis (B.th.), Paenibacillus alvei (P.al.),Staphylococcus aureus (S.aur.), Conidiobolus coronatus(C.cor.), Beauveria bassiana (B.bas.), Candida albicans (C.alb): from the absence of inhibition zones of test organisms (neutralism) to pronounced suppression zones with a width of more than 15 mm (antagonism); b — agar diffusion method for in vitro determination of antimicrobial activity against Staphylococcus aureus ATCC 25923. The numbers indicate strains: 1 — Lf5; 2 — Nm19; 3 — L5-1; 4 — L2-5; 5 — L4-2; 6 — Nm4; 7 — Lf15; 8 — Lf8; 9 — L2-6; 10 — L1-9. Рис. 4. АнтагонистическаЯ активность актиномицетов, ассоциированных с L. niger: a — тепловаЯ карта подавлениЯ роста Bacillus thuringiensis (B.th.), Paenibacillus alvei (P.al.), Staphylococcus aureus (S.aur.), Conidiobolus coronatus (C.cor.), Beauveria bassiana(B.bas.), Candida albicans (C.alb): от отсутствиЯ Зон ингибированиЯ тест-органиЗмов (нейтралиЗм) до выраженных Зон угнетениЯ Шириной более 15 мм (антагониЗм); b — определение антимикробной активности in vitro в отноШении Staphylococcus aureus ATCC 25923 методом диффуЗии в агар. Цифрами помечены Штаммы: 1 — Lf5, 2 — Nm19, 3 — L5-1, 4 — L2-5, 5 — L4-2, 6 — Nm4, 7 — Lf15, 8 — Lf8, 9 — L2-6, 10 — L1-9.
Fig. 3 in Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)
Fig. 3. Morphology of spore chains of the dominated streptomycete strains after incubation on ISP 3 agar at 28°C for 14 days: Streptomyces sp. L1-9 (a), Streptomyces sp. L2-5 (b), Streptomyces sp. L4-2 (c), Streptomyces sp. Lf5 (d), Streptomyces sp. Nm19 (e), Streptomyces sp. Nm4 (f). Рис. 3. МорфологиЯ цепочек спор доминируюЩих Штаммов стрептомицетов, выделенных иЗ муравьев Lasius niger, после инкубации в течении 14 дней на овсЯном агаре при 28°С: Streptomyces sp. L1-9 (a), Streptomyces sp. L2-5 (b), Streptomyces sp. L4-2 (c), Streptomyces sp. Lf8 (d), Streptomyces sp. Nm19 (e), Streptomyces sp. Nm4 (f).
Sweet Green. Alexanders, Smyrnium olusatrum, and Lasius niger, Garden Ant, Hortus Botanicus, Amsterdam, The Netherlands
<u>Source</u>: Flickr <br><u>4DCity URL</u>: <a href="https://4dcity.org/imgupload/1652210198.521.jpg">https://4dcity.org/imgupload/1652210198.521.jpg</a> <br><u>Original Image URL</u>: <a href="https://live.staticflickr.com/65535/52038170299_88070d0657_m.jpg">https://live.staticflickr.com/65535/52038170299_88070d0657_m.jpg</a> <br><br><u>Image-Metadata:</u><br>Filename: 1652210198.521.jpg<br>Image Dimensions: 239x239<br>Megapixels: 0.06 MP<br>Filesize: 18.34 KB<br><br>ExifOffset: 26
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