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10 results for “Live cell microscopy”
Live cell microscopy: From image to insight - raw data & analysis
<p>Accompanying raw and processed data as well as analysis scripts for the publication Biophysics Rev. 3, (2022); <a href="https://doi.org/10.1063/5.0082799">10.1063/5.0082799</a> "Live cell microscopy: From image to insight".</p>
Super-resolved Reflectance Confocal Microscopy data on Diatom shells and live MEF cells
<p>This dataset is linked to a paper submission in Open Research Europe. It contains the RAW data of confocal reflectance super-resolved point spread function, diatom shell zstack and time lapse imaging of a living Mouse Embryonic Fibroblast cell. </p>
Super-resolved Reflectance Confocal Microscopy time-lapse imaging of a living MEF cell lamellipod
<p>This movies presents a time-lapse of a label-free living Mouse embryonic fibroblast cell observed with super-resolved rescanned reflectance confocal microscopy.. </p>
Live-cell fluorescence microscopy data for: Structural Dynamics of the Functional Nonameric Type III Translocase Export Gate
<p><strong>yuan_t3ss_raw_data.zip:</strong></p> <p>Microscopy data types:<br> <strong>- </strong>Brightfield images<br> - Fluorescence images (514 nm excitation)</p> <p>Microscope:<br> Olympus IX-83 with 1.49NA oil-immersion objective (Olympus UAPON 100x)<br> ET442/514/561 Laser triple band set filtercube (69904, Chroma)</p> <p>Camera:<br> Hamamatsu C9100-13 EM-CCD Camera<br> 80 nm pixel size</p> <p><strong>yuan_t3ss_cell_objects.zip:</strong></p> <p>EPEC and C41 ColiCoords cell objects derived from the raw data. The set contains a total of 24979 individual cells (brightfield, binary, fluorescence and localization datasets for each cell) with corresponding coordinate systems.</p> <p>Code to generate these cell objects from the raw data: https://github.com/Jhsmit/T3SS-paper</p> <p>The data format is .hdf5 and can be read with any HDF5 reader or directly with ColiCoords: https://github.com/Jhsmit/ColiCoords</p> <p><strong>Contact:</strong><br> Jochem Smit</p>
Sample data for "Live Cell Fluorescence Microscopy – An End-to-End Workflow for High-Throughput Image and Data Analysis"
<p>This repository contains:</p> <ul> <li> <p>Sample data for the "Live Cell Fluorescence Microscopy – From Sample Preparation to Numbers and Plots" methodology paper by Zahumensky & Malinsky. The paper describes the preparation of live yeast cell samples for microscopy, the subsequent semi-automatic analysis of the microscopy images using our custom-written Fiji macros, and automatic processing of the output (Results table) from the image analys using custom-written R scripts. The data provided here are real experimental data from two publications of our group: Zahumensky et al., 2022 and Vesela et al., 2023</p> </li> <li> <p>"Results tables" from the Fiji based analysis</p> </li> <li> <p>Outputs of the processing of these Results tables using our R scripts, in the form of summary tables, graphs, and statistical analyses</p> </li> </ul>
Microscopy images and movies supporting the publication: "tRNA tracking for direct measurements of protein synthesis kinetics in live cells"
<p>This repository contains experimental and simulated microscopy movies and images supporting the publication: Volkov et al. (2018) tRNA tracking for direct measurements of protein synthesis kinetics in live cells. <em>Nat Chem Biol, </em>DOI: 10.1038/s41589-018-0063-y</p> <p>A detailed list of files and file organisation can be found in Repository_content.pdf.</p>
Data from: Label-free imaging of intracellular structures in living mammalian cells via external apodization phase-contrast microscopy
Open the record for dataset details and reuse information.
Single molecule microscopy reveals key physical features of repair foci in living cells
<p>Single Particle Tracking data of repair proteins in budding yeast, described in the manuscript entitled 'Single molecule microscopy reveals key physical features of repair foci in living cells'.</p>
The raw images of Laser Confocal Microscopy experiments in the manuscript: Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells
<p>The <strong>original imaging data</strong> folder contains the raw images of Laser confocal microscopy experiments in the manuscript: Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells. <a href="https://doi.org/10.1093/nar/gkac368">https://doi.org/10.1093/nar/gkac368</a> </p>
The raw images of Laser Confocal Microscopy experiments in the manuscript: Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells
<p>The <strong>original imaging data</strong> folder contains the raw images of Laser confocal microscopy experiments in the manuscript: Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells. <a href="https://doi.org/10.1093/nar/gkac368">https://doi.org/10.1093/nar/gkac368</a> </p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.