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Figure 5 in An integrative description of Mesobiotus ethiopicus sp. nov. (Tardigrada: Eutardigrada: Parachela: Macrobiotidae: harmsworthi group) from the northern Afrotropic region

Figure 5. Mesobiotus ethiopicus sp. nov. – PCM images of the midsection of various types of egg processes. Scale bars in µm.

Figure 6 in An integrative description of Mesobiotus ethiopicus sp. nov. (Tardigrada: Eutardigrada: Parachela: Macrobiotidae: harmsworthi group) from the northern Afrotropic region

Figure 6. Mesobiotus ethiopicus sp. nov. – SEM images of eggs: A–B – entire view of 2 different eggs; C–D – egg surface between processes; E–F – egg processes; G–H – a top part of the processes terminated by several short flexible filaments. Filled indented arrowheads indicate poorly developed connection between the processes, filled flat arrowheads indicate rare fully developed connections between processes. Scale bars in µm.

Figure 2 in An integrative description of Mesobiotus ethiopicus sp. nov. (Tardigrada: Eutardigrada: Parachela: Macrobiotidae: harmsworthi group) from the northern Afrotropic region

Figure 2. Mesobiotus ethiopicus sp. nov. – PCM images of the buccal apparatus: A – an entire buccal apparatus (paratype); B–C – the oral cavity armature of the holotype, dorsal and ventral teeth respectively; D–E – the oral cavity armature of the paratype, dorsal and ventral teeth respectively; F–G – the oral cavity armature of the paratype, dorsal and ventral teeth respectively; H–I – placoid morphology of the paratype, ventral and dorsal placoids, respectively. Filled flat arrowheads indicate the larger teeth in the second band of teeth, arrows indicate the medioventral tooth in the third band of teeth, empty flat arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in µm.

Figure 4 in An integrative description of Mesobiotus ethiopicus sp. nov. (Tardigrada: Eutardigrada: Parachela: Macrobiotidae: harmsworthi group) from the northern Afrotropic region

Figure 4. Mesobiotus ethiopicus sp. nov. – PCM images of the eggs: A – midsection; B – surface; C–D – surfaces under 1000× magnification. Asterisk indicates the wrinkled surface inside the areolae, filled indented arrowheads indicate poorly developed connection between the processes, filled flat arrowheads indicate rare fully developed connections between processes. Scale bars in µm.

Figure 1 in An integrative description of Mesobiotus ethiopicus sp. nov. (Tardigrada: Eutardigrada: Parachela: Macrobiotidae: harmsworthi group) from the northern Afrotropic region

Figure 1. Mesobiotus ethiopicus sp. nov. – PCM image of habitus: A – dorsoventral projection (holotype). Scale bar in µm.

Figure 3 in An integrative description of Mesobiotus ethiopicus sp. nov. (Tardigrada: Eutardigrada: Parachela: Macrobiotidae: harmsworthi group) from the northern Afrotropic region

Figure 3. Mesobiotus ethiopicus sp. nov. – PCM images of claws: A – claws I with smooth lunules; B – claws II with smooth lunules; C – claws IV with moderately serrated lunules. All claw photos from the holotype. Arrows indicate the cuticular bars, the filled arrowhead indicates the horseshoe structure connecting the anterior and the posterior claw. Scale bars in µm.

Fig. 8 in Integrative description of Mesobiotus anastasiae sp. nov. (Eutardigrada, Macrobiotoidea) and first record of Lobohalacarus (Chelicerata, Trombidiformes) from the Republic of South Africa

Fig. 8. Lobohalacarus cf. weberi, ♀ (SPbU 256(20)). A. Total view, black arrows indicate spines on genua I, PhC. B. Ventral shield, white arrowheads indicate bases of perigenital setae, PhC. C. Frontal spine, DIC. D. Genital opening, white arrowheads indicate genital acetabula, DIC. Scale bars: A = 100 µm; B = 50 µm; C = 10 µm; D = 5 µm.

Fig. 6 in Integrative description of Mesobiotus anastasiae sp. nov. (Eutardigrada, Macrobiotoidea) and first record of Lobohalacarus (Chelicerata, Trombidiformes) from the Republic of South Africa

Fig. 6. Mesobiotus anastasiae sp. nov., paratype (SPbU_Tar16). Eggs. A–C. Total view of the egg, SEM. D–F. Details of the egg surface, white arrowheads indicate pores above the collar, white arrows indicate pores below the collar, SEM. Scale bars: A–C = 20 µm; D–F = 5 µm.

Fig. 3 in Integrative description of Mesobiotus anastasiae sp. nov. (Eutardigrada, Macrobiotoidea) and first record of Lobohalacarus (Chelicerata, Trombidiformes) from the Republic of South Africa

Fig. 3. Mesobiotus anastasiae sp. nov., bucco-pharyngeal apparatus. A, E–O. Paratype, ♀ (SPbU 256(9)). B–D. Holotype, ♀ (SPbU 256(8)). A. Total dorso-ventral view of the bucco-pharyngeal apparatus, DIC. B. Ventral row of macroplacoids, focused on their outer surface, DIC. C. Ventral row of macroplacoids, focal plane shifted deeper into the parynx, black arrowheads indicate appendices of the third macroplacoid bent inward, DIC. D. Lateral rows of macroplacoids, black arrowheads indicate appendices of the third macroplacoid bent inward, DIC. E. Ventral row of macroplacoids, focused on their outer surface; note the absence of the preterminal constriction of the third macroplacoid, DIC. F–O. Oral cavity armature, dorsal (F–H, K–M) and ventral view (I–J, N–O), black arrowheads indicate teeth of the first band, F–J = PhC, K–O = DIC. Scale bars: A = 10 µm; B–O = 5 µm.

Fig. 2 in Integrative description of Mesobiotus anastasiae sp. nov. (Eutardigrada, Macrobiotoidea) and first record of Lobohalacarus (Chelicerata, Trombidiformes) from the Republic of South Africa

Fig. 2. Mesobiotus anastasiae sp. nov., cuticular sculpture. A. Paratype (SPbU 256(18)). Sculpture of the dorsal body surface, PhC. B–D. Paratype (SPbU_Tar16). B. High magnification of the sculpture of the dorsal body surface, SEM. C. Dorso-lateral zone of concentrated cuticular dots, SEM. D. Dot-like sculpture on the dorsal side of hind legs, SEM. Scale bars A, C, D = 5 µm; B = 2 µm.

Fig. 1 in Integrative description of Mesobiotus anastasiae sp. nov. (Eutardigrada, Macrobiotoidea) and first record of Lobohalacarus (Chelicerata, Trombidiformes) from the Republic of South Africa

Fig. 1. Mesobiotus anastasiae sp. nov., total view. A. Holotype, ♀ (SPbU 256(8)). Dorso-ventral view, PhC. B. Paratype (SPbU_Tar16). Lateral view in SEM. Scale bars = 50 µm.

Fig. 5 in Integrative description of Mesobiotus anastasiae sp. nov. (Eutardigrada, Macrobiotoidea) and first record of Lobohalacarus (Chelicerata, Trombidiformes) from the Republic of South Africa

Fig. 5. Mesobiotus anastasiae sp. nov., eggs. A–F. Paratype (SPbU 256(15)). G–I. Paratype (SPbU 256(4)). A. Total view of the egg surface, PhC. B. Total view of the optical section of the embryonated egg, PhC. C–D. Details of the egg surface, DIC, PhC. E. Optical section of the egg process, black arrowhead indicates pore, DIC. F–I. Optical sections of different egg processes, black arrowheads indicate collar, PhC. Scale bars: A–B = 20 µm; C–D = 10 µm; E–I = 5 µm.

Fig. 14 in Integrative description of two new species of the genus Mesobiotus (Eutardigrada, Macrobiotoidea) from Russia, with an updated phylogeny of the genus

Fig. 14. Phylogeny of Mesobiotus Vecchi, Cesari, Bertolani, Jönsson, Rebecchi & Guidetti, 2016 based on concatenated 18S + 28S + ITS-2 + COI sequences. Numbers at nodes indicate Bayesian posterior probability values (BI, first values) and bootstrap values (ML, second values). Black dots indicate the nodes supported by values of 1.0/100% with both methods. Low support values (below 0.9 in BI and below 70% in ML) not shown. Scale bar and branch lengths refer to the Bayesian analysis.

Fig. 13 in Integrative description of two new species of the genus Mesobiotus (Eutardigrada, Macrobiotoidea) from Russia, with an updated phylogeny of the genus

Fig. 13. Mesobiotus vulpinus sp. nov., paratype (SPbU Tar_65), egg. A–B. Details of the egg processes, whire arrowheads indicate large pores, black arrowheads indicate small pores, SEM. Scale bars = 5 µm.

Fig. 12 in Integrative description of two new species of the genus Mesobiotus (Eutardigrada, Macrobiotoidea) from Russia, with an updated phylogeny of the genus

Fig. 12. Mesobiotus vulpinus sp. nov., egg. A–D. Paratypes (SPbU Tar_65). A, C. Total view of the eggs, SEM. B, D. Details of the egg surface, SEM. Note the difference in the degree of development of small tubercles and numerous small pores on the egg surface. Scale bars: A–B = 20 µm; C–D = 5 µm.

Fig. 11 in Integrative description of two new species of the genus Mesobiotus (Eutardigrada, Macrobiotoidea) from Russia, with an updated phylogeny of the genus

Fig. 11. Mesobiotus vulpinus sp. nov., paratype (SPbU 320(6)), egg. A. Total view of the egg, PhC. B. Egg processes with small bulbous process between them, black arrowhead, DIC. C–D. Details of the egg surface, black arrowheads indicate small bulbous processes, PhC (C), DIC (D). E. Bifurcated egg process, PhC. F. Egg process with "bubble", black arrowhead, PhC. G–H. Optical sections of the egg process basal part, white arrowheads indicate a pore, DIC. Scale bars: A = 20 µm; B–H = 10 µm.

Fig. 9 in Integrative description of two new species of the genus Mesobiotus (Eutardigrada, Macrobiotoidea) from Russia, with an updated phylogeny of the genus

Fig. 9. Mesobiotus vulpinus sp. nov., bucco-pharyngeal apparatus. A–G. Holotype (SPbU 320(10)). H. Paratype (SPbU 320(1)). A. Total dorso-ventral view of the bucco-pharyngeal apparatus, PhC. B–C. Placoids, black arrowheads indicate the preterminal constriction of the third macroplacoid, PhC (B), DIC (C). D–G. Oral cavity armature (D–E = dorsal view, F–G = ventral view), PhC (D, F), DIC (E, G). H. Oral cavity armature with fragmented medio-ventral ridge, PhC. Scale bars: A = 20 µm; B–H = 10 µm.

Fig. 10 in Integrative description of two new species of the genus Mesobiotus (Eutardigrada, Macrobiotoidea) from Russia, with an updated phylogeny of the genus

Fig. 10. Mesobiotus vulpinus sp. nov., claws. A, C, F–G. Paratype (SPbU Tar_33). B. Paratype (SPbU 320(1)). D, H. Holotype (SPbU 320(10)). E. Paratype (SPbU 320(5)). A. Inner surface of leg III, white arrowhead indicates indistinctly marked pulvinus, SEM. B. Claws of leg II, black arrowhead indicates bar-like cuticular thickening, PhC. C. Claws of leg III, SEM. D. Claws of leg I, focused on bar-like cuticular thickening, black arrowhead, white arrowhead indicates the zone of dot-like sculpture, PhC. E. Claws of leg IV, black arrowheads indicate cuticular sculpture around the claw bases, PhC. F. Claws of leg IV, SEM. G. Lunules of claws of leg IV, SEM. H. Leg IV, focused on horseshoe-like structure, white arrowhead, black arrowhead indicates cuticular sculpture around the claw base, PhC. Scale bars: A–B, D–E, H = 10 µm; C, F–G = 5 µm.

Fig. 8 in Integrative description of two new species of the genus Mesobiotus (Eutardigrada, Macrobiotoidea) from Russia, with an updated phylogeny of the genus

Fig. 8. Mesobiotus vulpinus sp. nov., cuticular sculpture and oral cavity armature (OCA). A–B, E–F. Paratype (SPbU Tar_33). C–D. Holotype (SPbU 320(10)). A. High magnification of the sculpture of the dorsal body surface, SEM. B. Dot-like sculpture on the external surface of leg III, SEM. C. Dotlike sculpture on the external surface of leg III, PhC, black arrowhead inticates the zone of sculpture. D. Dot-like sculpture on the dorsal side of hind leg, PhC. E. Dot-like sculpture on the dorsal side of hind leg, SEM, white arrowhead indicates the zone of sculpture. F. Mouth opening with dorsal OCA visible, SEM, white arrow indicates the first band of teeth, white arrowhead indicates the dorsal crests of the third band of teeth. Scale bars A–B, F = 2 µm; C–E = 5 µm.

Fig. 7 in Integrative description of two new species of the genus Mesobiotus (Eutardigrada, Macrobiotoidea) from Russia, with an updated phylogeny of the genus

Fig. 7. Mesobiotus vulpinus sp. nov., total view. A. Paratype, ♀ (SPbU 420(1)). Dorso-ventral view, PhC. B. Paratype (SPbU Tar_65). Ventro-lateral view in SEM. Scale bars = 50 µm.