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11,869 results for “Metabolism”
Pelagic, epilimnetic production estimates in Sparkling, Trout (Wisconsin), Acton (Ohio), and Castle (California) Lakes (USA) calculated using 14C and free-water O2 metabolism methods, 2007 - 2017
Concurrent daily estimates of pelagic, eplilimnetic production (mmol C m3 d) generated from 14C incubations and diel changes in high frequency dissolved oxygen data (free-water). Original data derived from the North Temperate Lakes Long Term Ecological Research program (Sparkling [2007-2013], Trout [2007-2012] Lakes), Castle Lake Research Station (Castle Lake [2014-2017]), and Center for Aquatic and Watershed Sciences (Acton Lake [2010-2014]). 14C production estimates were generated as part of each research programs core data collection. Free-water production estimates generated using high frequency sensor data provided by research programs and Phillips (2020) time-varying, Bayesian metabolism model.
Free water metabolism (FWM) derived net ecosystem production (NEP) from lagoon sites along the Beaufort Sea Coast, Alaska, 2019-ongoing
Continuous measurements of dissolved oxygen (DO) in order to calculate daily values of free water metabolism (FWM) derived net ecosystem production (NEP) at several core Beaufort Lagoon Ecosystems LTER (BLE-LTER) stations. These deployments were done in addition to year-round core program BLE-LTER mooring deployments during the under ice sampling at surface and bottom depths (April/May), and during open water season in bottom depths (July/August). Gross primary production (GPP), ecosystem respiration (ER), and the sum of the two: net ecosystem production (NEP) are reported in mol C m-2 day-1.
Daily summer riverine and groundwater pCO2, dissolved oxygen, and ecosystem metabolism from sites along the Alaska Beaufort Sea coast, 2019-2022
Three riverine systems near Utqiaġvik, Alaska, are sampled regularly by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program in order to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Sampling includes one intermediate river, Avak Creek, and two smaller streams: the Mayoeak River (thermokarst stream) and a beaded stream. In 2019, 2021, and 2022, we performed additional sampling at these core program sites, with continuous monitoring of the streams between late June and August. Continuous measurements included riverine discharge, pCO₂, dissolved oxygen (DO), and ecosystem production, along with periodic grab samples of dissolved organic carbon (DOC). In 2022, there was also monitoring of groundwater wells at the two streams, capturing continuous hydraulic head and groundwater pCO₂, in addition to grab samples for DOC. Daily averages of continuous data and DOC laboratory results are presented for surface water and groundwater. This dataset is not part of the BLE Core Program and is not currently planned as an ongoing effort.
Experimental soil metabolism responses to oxygen availability and carbon pulses
Soil metabolism rates were measured in experimental flow-through reactors (FTRs) in order to assess responses to oxygen availability (i.e., redox conditions) and pulsed inputs of bioavailable carbon. The oxygen and carbon manipulations were meant to simulate patchy conditions in the rhizosphere of Spartina alterniflora marshes. The experiment was conducted over 97 days. During the first 84 days, pulses were applied weekly and metabolic responses were measured 1 and 5 days later. After the final carbon pulse, metabolism responses were followed for another 13 days in order to assess starvation responses. We used soils from Airport marsh on Sapelo Island.
Rates of benthic metabolism and nutrient cycling in the Parker and Rowley Rivers of the Plum Island Sound estuary, Massachusetts, PIE LTER.
Rates of benthic metabolism and nutrient cycling in the Parker and Rowley Rivers of the Plum Island Sound estuary. Measurements include those conducted at two sites in the Parker River in Spring (high river discharge) and Fall (low discharge) for long-term monitoring, also at other sites throughout the estuary over a variety of seasons and salinities.
High-frequency dissolved oxygen, water temperature, wind speed, and radiation data; stream and in-lake nutrient concentration data; and daily metabolism and nutrient loading estimates for 16 lakes in North America and Northern Europe.
In lakes, ecosystem structure and processes are influenced by gross primary production (GPP), ecosystem respiration (R), and net ecosystem production (NEP). The rates of these metabolic processes are often controlled by resource availability, which often reflects catchment loads. Although the relationship between catchment loads and in-lake nutrient concentrations may be well defined in specific lakes, we explored how watershed vs. in-lake predictors of metabolism compare across lake types. To do this, we combined stream loads of carbon (C), nitrogen (N), and phosphorus (P) with high frequency in situ monitoring of lake metabolism and in-lake C, N, and P concentrations from 16 lakes spanning a range of latitudes (39 to 64 degrees N), inflowing stream (0 - 6 streams), and trophic status (oligotrophic to eutrophic). The data package includes high-frequency dissolved oxygen, water temperature, wind speed, and solar radiation data as well as daily estimates of GPP, R, and NEP derived from those data. In addition, the data package includes in-lake and stream concentrations of dissolved organic carbon, total nitrogen, and total phosphorus and stream discharge data. The package also includes estimates of daily carbon, nitrogen and phosphorus loading to each lake derived from the stream concentrations and discharge.
Metabolism and water chemistry of tundra ponds near Utqiagvik, Alaska, 2019–2024
Low centered polygon ponds and thermokarst trough ponds were sampled near Utqiagvik, AK in late summer from 2009-2024 to understand how pond types differ in chemistry and C flux. Water samples from open water were characterized for nutrients concentration and algal biomass. Carbon and oxygen concentrations were monitored continuously using sensors.
Lake Metabolism at North Temperate Lakes LTER 2000
Recent literature suggests that for many lakes and rivers, the respiratory breakdown of organic matter (R) exceeds production of organic matter by photosynthesis (gross primary production; GPP) within the water body. This metabolic balance (GPP less than R; heterotrophy ) implies that allochthonous organic matter supports a portion of the aquatic ecosystems respiration. Evidence that many lakes are heterotrophic comes from diverse approaches, and debate remains over the circumstances in which heterotrophy exists. The methods used to estimate GPP and R and the limited extent of lake types studied, especially with respect to dissolved organic carbon (DOC) and total phosphorus (TP) concentrations, are two reasons for differing conclusions. In this study, O2 and CO2 sondes were deployed during July and August, 2000 to measure diel gas dynamics in the surface waters of 25 lakes in the Northern Highland Lake district of Wisconsin and the Upper Peninsula of Michigan. The lakes were chosen to span wide and orthogonal ranges in DOC and TP concentrations. From these data, we calculated GPP, R and net ecosystem production (NEP=GPP-R). Over the broad range in TP and DOC among the lakes, diel CO2 and O2 changed on a near 1:1 molar ratio. Metabolism estimates from the two gases were comparable, except at high pH. Most lakes in our data set had -NEP, but GPP and R appeared to be controlled by different factors. TP correlated strongly with GPP, whereas DOC correlated with R. At low DOC concentrations, GPP and R were nearly equal, but at higher DOC, GPP and R uncoupled and lakes had -NEP. Strong correlations between lake metabolism and landscape related variables suggest that allochthonous carbon influences lake metabolism. Sampling Frequency: Chemical parameters and physical properties sampled from 1 to 4 times during the summer. Time series data step is 30 minutes. Number of sites: Time series data for 25 lakes. Chemical and physical data from 31 lakes.
Fluxes project at North Temperate Lakes LTER: Spatial Metabolism Study 2007
Data from a lake spatial metabolism study by Matthew C. Van de Bogert for his Phd project, "Aquatic ecosystem carbon cycling: From individual lakes to the landscape." The goal of this study was to capture the spatial heterogeneity of within-lake processes in effort to make robust estimates of daily metabolism metrics such as gross primary production (GPP), respiration (R), and net ecosystem production (NEP). In pursuing this goal, multiple sondes were placed at different locations and depths within two stratified Northern Temperate Lakes, Sparkling Lake (n=35 sondes) and Peter Lake (n=27 sondes), located in the Northern Highlands Lake District of Wisconsin and the Upper Peninsula of Michigan, respectively.Dissolved oxygen and temperature measurements were made every 10 minutes over a 10 day period for each lake in July and August of 2007. Dissolved oxygen measurements were corrected for drift. In addition, conductivity, temperature compensated specific conductivity, pH, and oxidation reduction potential were measured by a subset of sondes in each lake. Two data tables list the spatial information regarding sonde placement in each lake, and a single data table lists information about the sondes (manufacturer, model, serial number etc.). Documentation:Van de Bogert, M.C., 2011. Aquatic ecosystem carbon cycling: From individual lakes to the landscape. ProQuest Dissertations and Theses. The University of Wisconsin - Madison, United States -- Wisconsin, p. 156. Also see Van de Bogert, M.C., Bade, D.L., Carpenter, S.R., Cole, J.J., Pace, M.L., Hanson, P.C., Langman, O.C., 2012. Spatial heterogeneity strongly affects estimates of ecosystem metabolism in two north temperate lakes. Limnology and Oceanography 57, 1689-1700.
Modeling dataset: Long-term Change in Metabolism Phenology across North-Temperate Lakes, Wisconsin, USA 1979-2019
This dataset includes model configurations, scripts and outputs to process and recreate the outputs from Ladwig et al. (2021): Long-term Change in Metabolism Phenology across North-Temperate Lakes. The provided scripts will process the input data from various sources, as well as recreate the figures from the manuscript. Further, all output data from the metabolism models of Allequash, Big Muskellunge, Crystal, Fish, Mendota, Monona, Sparkling and Trout are included.
Integration of sweet taste and metabolism determines carbohydrate reward-study 3
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Short-term consumption of sucralose with, but not without, carbohydrate impairs neural and metabolic sensitivity to sugar
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Four lipidomics datasets (mouse liver, mouse pancreatic islets, mouse soleus muscle and mouse visceral adipose tissue), generated for the publication Mehl et al., "A multiorgan map of metabolic, signalling, and inflammatory pathways that coordinately control fasting glycemia in mice"
<p>Mehl, Thorens et al present a multiomics study aimiing to<span> identify the pathways that are coordinately regulated in pancreatic </span><span>b</span><span>-cells, muscle, liver, and fat to control fasting glycemia we fed C57Bl/6, DBA/2 and Balb/c mice a regular chow or a high fat diet for 3, 10 and 30 days. We measured fasted glycemia, insulinemia and whole-body insulin resistance. Transcriptomic and lipidomic analysis were used in a data fusion approach to identify organ-specific pathways related to the glycemic levels across all conditions investigated. In pancreatic islets, constant insulinemia despite higher glycemic levels were associated with reduced expression of mRNAs encoding hormone and neurotransmitter receptors as well as OXPHOS, cadherins, integrins and gap junction proteins. Higher glycemia and whole-body insulin resistance were associated, in muscle, with reduced expression of mRNAs encoding insulin signaling proteins and enzymes of the glycolysis, Krebs’ cycle and OXPHOS pathways, as well as endocytosis and exocytosis proteins; in hepatocytes, with lower expression of mRNAs of the insulin signaling pathway, of branched chain amino acid catabolism and of OXPHOS; in adipose tissue, with increased expression of mRNAs of innate immunity and lipid catabolism. These data provide a map of the pathways that are coordinately recruited in the investigated tissues to control fasting glycemia and a resource for further studies of interorgan communication in glucose homeostasis. </span></p>
Ecosystem metabolism and associated environmental data for a forested, meadow and reforested reach of White Clay Creek, Chester Co., Pennsylvania; 1971-1975 and 1997-2010
Ecosystem metabolism data for a 3rd-order Piedmont stream were collected during two periods: P1- April 1971 – Dec 1975, and P2- May 1997 – January 2010. Measures were made in a meadow and a forested reach during each period and in a reforested (formerly meadow) reach during the latter years of P2. During P1, measures were made by transferring streambed substrata to chambers in water jackets located on the streambank and measuring dissolved oxygen changes over diel periods. During P2, open system measures of dissolved O2 change were made for several days in warm and cold seasons, with reaeration determined from a propane injection experiment. Metabolism estimates were determined from diel curves of dissolved O2 change. Photosynthetically active radiation (PAR) and chlorophyll were measured concurrent with many measurements in P1 and all measures during P2, and temperature with all measures. Water chemistry parameters (NH4-N, NO3-N, PO4-P, SiO2, Cl, SO4, total alkalinity, pH) associated with each run are included in the data set, as are days since storm of various thresholds. Field procedures, analytical methods and data analyses are detailed in Bott, T.L. & J. D. Newbold, 2023. A multi-year analysis of factors affecting ecosystem metabolism in forested and meadow reaches of a Piedmont Stream. Hydrobiologia
Metabolism dataset: one year of high-frequency temperature, dissolved oxygen, wind, photosynthetically active radiation observations and low-frequency nutrient data for 58 lakes in the Global Lake Ecological Observatory Network
Understanding controls on primary productivity is essential for describing ecosystems and their responses to environmental change. Lake primary production is strongly controlled by inputs of nutrients and colored dissolved organic matter. While past studies have developed mathematical models of this nutrient-color paradigm, broad empirical tests of these models are scarce. We compiled data from 58 diverse and globally distributed and mostly temperate lakes to test such a model and improve understanding and prediction of the controls on lake primary production. These lakes varied widely in size (0.02-2300 km2), pelagic gross primary production (20-8000 mg C m-2 d-1), and other characteristics. The data package includes high-frequency dissolved oxygen, water temperature, wind speed, and solar radiation data as well as daily estimates of GPP and ER derived from those data. In addition, the data package includes median in-lake and stream concentrations of dissolved organic carbon and total phosphorus for a subset of 18 of those lakes.
Data in support of 'Mechanistic insights into plant community responses to environmental variables: genome size, cellular nutrient investments, and metabolic trade-offs.'
Data was collected to examine whether and how the plant genome size (GS) influences traits (stomata size, stomata density, cellular and tissue level carbon (C), nitrogen (N), and phosphorus (P) contents) and metabolic-tradeoffs (of photosynthesis, evapotranspiration, water-use, efficiency) of plants in treatment plots in which nothing, N, P, or NP had been annually added. Data was collected from ~500 plants from seven grassland sites that are all part of the Nutrient Network (https://nutnet.org), a globally distributed experiment in which plots have different nutrient amendment treatments that are administered identically to allow cross-site comparisons of the effects of nutrients on biodiversity patterning. The sites chosen varied along a North-South latitude, longitude, mean annual precipitation (MAP) and mean annual temperature (MAT) gradient.
Daily river metabolism using oxygen flux at 75 sites in Mongolia or the United States in steppe ecoregions
We obtained GIS data to indicate local geomorphology and watershed-scale values for land use, climate, slope, and elevation for each sampling site. We selected our sites using the GIS-based program RESonate (Williams et al., 2013) to represent replicates in multiple watersheds of different geomorphic patches or Functional Process Zones (FPZs). The FPZs are reoccurring longitudinal geomorphic patches that are hypothesized to control biocomplexity, including community composition and system productivity (Thorp et al., 2006). A detailed description of the FPZ delineation methodology we employed has been provided previously (Maasri et al., 2019a; Erdenee et al., 2021). We classified each study site hierarchically by country, ecoregion, river basin, upper (streams higher in the watershed) or lower (low slope rivers of lower elevations), and relatively constrained valley or wide valley. This approach allowed us to assess reach-scale properties that could directly influence the physiological controls most often collected alongside metabolism data. This provided a framework to evaluate how we may understand the determinants of metabolism at multiple scales. We studied three large-scale temperate steppe ecoregions (Terminal Basin, TB; Montane Steppe, MS; and Grassland Steppe, GS) as characterized by Olson et al. (2001) and updated by Dinerstein et al. (2017) in two countries (Mongolia and the United States, Fig. 2). We aggregated our large-scale ecoregions for the US as follows: TB = Great Basin shrub steppe and Sierra Nevada forest, MS = South Central Rockies forest and Wyoming Basin shrub steppe, GS = Nebraska Sand Hills mixed grasslands and Northern Shortgrass prairie. We aggregated our large-scale ecoregions for Mongolia as follows: TB = Altai mountains forest and forest steppe, Gobi Lakes Valley desert steppe, Great Lakes Basin desert steppe, and Khangai Mountains alpine meadows, MS = Selenge-Orkhon forest steppe and Syan Mountains conifer forests, GS = Daurian Forest s
Population persistence, phenotypic divergence and metabolic adaptation in yarrow (Achillea millefolium L.) along a climate gradient, CA, 1920 to 2023
This dataset provides insights into the persistence and adaptation of yarrow (Achillea millefolium L.) populations over a 100-year period of climate change. The data include plant height measurements and climatic variables (temperature and precipitation) from historical and resurveyed sites spanning a broad environmental gradient (1–3,200 m a.s.l.), alongside metabolic profiles obtained from a common-garden experiment. The dataset captures phenotypic changes in plant growth, metabolic diversity, and site-specific climatic shifts between 1920 and 2020. These data support analyses of how temperature and precipitation interact to shape plant responses over time and allow for exploring patterns of local adaptation in phenotypic and metabolic traits. This comprehensive dataset is valuable for understanding the ecological and evolutionary mechanisms underlying population persistence and can inform conservation strategies under future climate scenarios.
Ecosystem metabolism and associated modeling parameters for 6 oligotrophic lakes and ponds in a single watershed
This dataset was collected as part of a watershed-scale study to assess impacts of smoke cover on water temperature and rates of ecosystem metabolism in small lakes and ponds. We measured thermal and metabolic responses to smoke in six waterbodies within a high-elevation watershed (watershed area 1908 ha; elevation range 2800-3229 m.a.s.l) located in Sequoia-Kings Canyon National Park in the Sierra Nevada Mountains of California. All lakes and ponds are oligotrophic, and range in maximum depth from 1.5m to 10m. The dataset includes: time series data of dissolved oxygen, water temperature, and environmental parameters relevant to modeling ecosystem metabolism; estimated rates of ecosystem metabolism using the Kalman filter method; descriptive site information including location, size, and depth.
Nearshore high-frequency temporal water quality observations and process-based modeling of aquatic ecosystem metabolism in Lake Tahoe completed by members of the Blaszczak Lab at the University of Nevada Reno, 2021-2023
The overarching goal of this project was to develop a process-based understanding of how watershed-to-lake connections drive nearshore productivity dynamics in a large oligotrophic mountain lake (Lake Tahoe). We addressed this goal through a combined approach of high-frequency sensor deployment and maintenance, ecosystem metabolism modeling, laboratory incubations, and routine monitoring of water chemistry and other parameters. The data we collected as part of this project and the ecosystem metabolism estimates we generated demonstrate how variable ecosystem productivity is in time and space in the nearshore of Lake Tahoe. Although maintenance of the sensor arrays during the exceptional winter of 2023 was challenging, we were able to capture the data necessary to estimate a complete time series of metabolic activity across two years with very different hydroclimatic conditions. Throughout this project we accomplished the following: 1. We generated over two years of daily estimates of ecosystem metabolism (gross primary productivity, ecosystem respiration, and net ecosystem productivity) from multiple locations on both the east and west shores of the lake and from areas in close proximity to and far away from stream water inflows. 2. We measured ammonium (NH4+) and nitrate (NO3-) concentrations in surface water samples from both Glenbrook and Blackwood creeks and the nearshore of Lake Tahoe for over two years. 3. We quantified rates of NH4+ and NO3- uptake in benthic samples of the dominant substrate type collected during peak streamflow, the receding limb, and baseflow conditions in 2023 from multiple locations in the nearshore using established laboratory incubation methods. 4. Finally, we used a combination of time series models and structural equation modeling to integrate our results and improve understanding of the direct and indirect effects of hydroclimatic variability on observed patterns in ecosystem metabolism in the nearshore. See this git code repository
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Allen Brain Atlas
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Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
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The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
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