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26 results for “Microsporidia”
Supplementary Data for the manuscript 'The Rad9-Rad1-Hus1 DNA repair clamp is found in Microsporidia'
<p>This dataset contains the supplementary data files for the manuscript titled 'The Rad9-Rad1-Hus1 DNA repair clamp is found in Microsporidia'.</p>
Fig. 1 in Spore Dimorphism in Nosema pyrausta (Microsporidia, Nosematidae): from Morphological Evidence to Molecular Genetic Verification
Fig. 1. DAPI fluorescence (A, С) and Nomarski contrast (B, D) of monokaryotic (A, B) and diplokaryotic (C, D) spores of microsporidia detected in Ostrinia nubilalis larvae. Arrows and double arrows indicate single nuclei and diplokarya, respectively. Scale bar = 4 µm.
Fig. 4 in High temperatures and low humidity promote the occurrence of microsporidians (Microsporidia) in mosquitoes (Culicidae)
Fig. 4 Occurrence of microsporidian species found in total (All), male and female mosquitoes collected during the cold and warm months. Comparative statistics are presented in Additional file 1: Tables S12 and S13
Fig. 3 in High temperatures and low humidity promote the occurrence of microsporidians (Microsporidia) in mosquitoes (Culicidae)
Fig. 3 Raincloud plots (i.e. combined violin plots, box plots and dot plots) for the contribution of infected mosquitoes relative to cold or warm months: a for the mosquito pool without dividing into females and males and b separately for females and males, collected in cold (blue) and warm (red) months. The dot plots show the proportion of infected mosquitoes (jittered horizontally). Each dot is the individual proportion of a particular species with a particular sex in a single month. The box plots show the extremes (whisker tails), interquartile range (box boundaries) and median (horizontal line). The violin plots show the probability density of the data. Symbols (asterisks or 'ns') indicate Bonferroni p-value (B) and Holm p-value (H) (B/H). Double asterisks (**) indicate statistical significance at p <0.01; ns, no statistical significance
Fig. 2 in High temperatures and low humidity promote the occurrence of microsporidians (Microsporidia) in mosquitoes (Culicidae)
Fig. 2 Correlation plots between microsporidian DNA-positive mosquitoes and a temperature (°C), b humidity (%), c wind (m/s) and d rainfall (mm). The R value indicates the Pearson's correlation coefficient statistic, the p value is statistically significant, the shadowed area shows the 95% confidence interval, and the black line is the regression line. The month and year of the data points are indicated according to the legend at the bottom
Fig. 3 in Ultrastructure and Phylogeny of Pleistophora beebei sp. nov. (Microsporidia) Infecting the Amazonian Teleostean Brachyhypopomus beebei (fam. Hypopomidae)
Fig. 3. Maximum Likelihood tree showing the relationship of Pleistophora beebei sp. nov. to other microsporidians based on the rDNA sequences. The numbers on the branches are bootstrap confidence levels on 500 replicates for ML trees. The tree was generated using 34 microsporidian selected sequences, with Potaspora morhaphis as the outgroup species. The bar indicates the equivalence between the distance and the number of changes. GenBank accession numbers are in parenthesis after the species name. There were a total of 966 positions in the final dataset.
Figs 15–17 in Microsporidia in a Woodland Pool I. Lanatospora costata sp. n. (Opisthosporidia, Microsporidia), Parasite of Megacyclops viridis (Crustacea, Copepoda): Fine Structure and Molecular Phylogeny
Figs 15–17. Lanatospora costata, parasite of Megacyclops viridis, structure of spores as seen in SEM and TEM. 15 – Spore surface ornamentation as seen by SEM. Note that the exospore ribs form a complex armour on the spore surface. Scale bar: 1 µm. 16 – Detail of the polaroplast lamellae (pl) in the apical part of the spore, pf – polar filament. Scale bar: 200 nm. 17 – Details of the polar filament coils (pf) in cross section. Scale bar: 500 nm.
Fig. 3 in New Microsporidia, Glugea sardinellensis n. sp. (Microsporea, Glugeida) Found in Sardinella aurita Valenciennes, 1847, Collected off Tunisian coasts
Fig. 3. Maximum likelihood phylogenetic tree based on the SSU rDNA data set selected microsporidian species showing the position of Glugea sardinellensis sp. n. Bootstrap supports based on 1,000 replicates from Maximum likelihood/neighbour joining analysis are indicated at each node. GenBank accession numbers for each species are reported in parenthesis. Brachiola algerae was used as outgroup. The scale bar shows the number of changes per site.
Fig. 2 in New Microsporidia, Glugea sardinellensis n. sp. (Microsporea, Glugeida) Found in Sardinella aurita Valenciennes, 1847, Collected off Tunisian coasts
Fig. 2. Ultrastructural aspects of the developmental stages of G. sardinellenesis n. sp. (A) unincleated sporoblast, (B) binucleated sporoblast, (C) immature spore, (D) mature spore showing anchoring disc (AD), polar filament (PF), lamellar polaroplast (Pb), posterior vacuole (V), nucleus (N), exospore (Ex) and endospore (En). Scale bars: 1 µm.
Fig. 2 in Ultrastructure and Phylogeny of Pleistophora beebei sp. nov. (Microsporidia) Infecting the Amazonian Teleostean Brachyhypopomus beebei (fam. Hypopomidae)
Fig. 2. Semi-schematic drawings of a macrospore (A) and a microspore (B). (The scale bar corresponds to the two schematic drawings).
Fig. 19 in Microsporidia in a Woodland Pool I. Lanatospora costata sp. n. (Opisthosporidia, Microsporidia), Parasite of Megacyclops viridis (Crustacea, Copepoda): Fine Structure and Molecular Phylogeny
Fig. 19. The woodland pool near Přerov nad Labem, Central Bohemia Region, Czech Republic (50°167′N, 14°810′E), the type habitat of Lanatospora costata sp. n.
Fig. 1. Glugea sardinellensis n in New Microsporidia, Glugea sardinellensis n. sp. (Microsporea, Glugeida) Found in Sardinella aurita Valenciennes, 1847, Collected off Tunisian coasts
Fig. 1. Glugea sardinellensis n. sp. infecting Sardinella aurita. (A–B) Infected round sardinella showing different sized xenomas in the pyloric caeca (arrows) (scale bar: 1 cm). (C) Fresh spores of G. sardinellensis n. sp. (scale bar: 5 µm). (D–E) Semi-thin section of the pe- ripheral region of the xemona showing the wall (XW) and numerous spores (scale bar: 20 µm). Cf – collagen fibers; Fb – fibroblast cells.
Fig. 11 in Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the Beet Webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia
Fig. 11. Molecular phylogenetics of Tubulinosema loxostegi and related taxa, as obtained by Bayesian inference (BI) and Maximum likelihood (ML) from an alignment of respective ribosomal RNA gene sequences listed in Table 1. The branch support is given as posterior probability for BI and bootstrap value for ML. The branch length for the outgroup Janacekia debaisieuxi is reduced twofold (double slash). Scale bar: 0.01 expected changes per site.
Figs 9–10 in Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the Beet Webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia
Figs 9–10. Electron microscopy of abberant spores of Tubulinosema loxostegi. 9 – a spore with the prominent protrusion of the anchoring disc (arrow), characteristic of spore activation (perhaps due to the fixative); 10 – an oversized teratospore with irregularly laid layers of ER, amorphous matter (asterisk) and tubules (arrows). Abbreviations as in Figs 3–8. Scale bars: 1 µm.
Figs 3–8 in Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the Beet Webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia
Figs 3–8. Electron microscopy of Tubulinosema loxostegi. 3 – the sporoblast with tubules on its surface; 4 – exospore of the immature spore with short spiky extensions (arrow); 5 – mature spore within the cytoplasm of the host cell showing the disposition of bipartite polaroplast, the polar tube and the nucleus; 6 – the anterior part of the spore demonstrating structure of the anchoring disc and the polaroplast, as well as the additional layer of the exospore (arrow); 7 – the polar tube coils, with posterior coils of lesser diameter; 8 – two spores showing the diplokaryotic arrangement of the nuclei and delamination of the outer layer of the exospore (arrow), possibly due to an artifact of poor tissue preservation. AD – anchoring disc, En – endospore, ER – endoplasmatic reticulum, Ex – exospore, N – nuclei, Pp1 – anterior part of the polaroplast, Pp2 – posterior part of the polaroplast, PT – polar tube, PT1 – anterior coils of PT, PT2 – posterior coils of PT, Tb – tubules. Scale bars: 1 µm (3, 5, 8), 0.5 µm (4, 6, 7).
Fig. 1 in Bombus brasiliensis Lepeletier (Hymenoptera, Apidae) infected with Nosema ceranae (Microsporidia)
Fig. 1. (A) The 32 surveyed localities in northeastern Argentina. Formosa province [Fo]: (1) Ingeniero Juárez; (2) Laguna Yema; (3) Las Lomitas; (4) Bañado La Estrella; (5) Posta Cambio Zalazar; (6) Pozo del Tigre; (7) Colonia Perin; (8) Ibarreta; (9) Palo Santo; (10) Pirané; (11) Gran Guardia; (12) El Colorado. Chaco province [Ch]: (13) J.J. Castelli; (14) Presidencia Roque Saenz Peña; (15) Colonia Elisa; (16) 38 km North of Resistencia; (17) Resistencia. Corrientes province [Co]: (18) Corrientes; (19) Estero Santa Lucía; (20) Colonia Carlos Pellegrini; (21) Laguna Iberá; (22) Santo Tomé. Misiones province [Mi]: (23) Posadas; (24) Leandro N. Alem; (25) Cuña Pirú; (26) Aristóbulo del Valle; (27) El alcázar; (28) Montecarlo; (29) 30 km East of María Magdalena; (30) Wanda; (31) Urugua-í. (Ɨ) indicates Puerto Iguazú, the only locality where six Bombus brasiliensis workers were found. (B) Habitat where B. brasiliensis was found. (C) Two distended midgut cells of B. brasiliensis with spores of Nosema ceranae inside.
Fig. 2 in Wide geographic distribution of overlooked parasites: Rare Microsporidia in Gammarus balcanicus, a species complex with a high rate of endemism
Fig. 2. Bayesian phylogenetic reconstruction of Microsporidia based on partial small ribosomal subunit rDNA alignment (Supplementary data 1). Labels in bold and in blue frames are parasites of Gammarus balcanicus found in the present study. These labels show the name of the parasite (in case of described species) or in the case of undescribed taxa the name consist of: M. sp (= Microsporidium sp.) followed by clade number sensu Vossbrinck and Debrunner-Vossbrinck (2005), MOTU, haplogroup number (e.g. b01, b02), then the country where it was found (two letter ISO code, see Table S1), the number of infected populations (=pop.), and the total number of infected individuals (=ind.). Labels with accession numbers are parasite sequences taken from GenBank. These labels show the accession number, the parasite name given in the associated publication, the order of the host (except for amphipod hosts where the family is provided). Microsporidia clade numbers are as in Vossbrinck and Debrunner-Vossbrinck (2005). Branches are collapsed for the two genera Nosema and Dictyocoela (triangle sizes not reflecting actual size). Abbreviation: PP, Bayesian posterior probability. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Fig. 1 in Wide geographic distribution of overlooked parasites: Rare Microsporidia in Gammarus balcanicus, a species complex with a high rate of endemism
Fig. 1. Gammarus balcanicus sampling sites. Sites are identified by black dots with numbers as in Table S1 (87). See Additional Table S1 for details (e.g. sampling sizes, GPS coordinates). Countries identified with ISO code. Map created by authors using Qgis 2.18.4 (QGIS Development Team 2009).
Fig. 3 in Wide geographic distribution of overlooked parasites: Rare Microsporidia in Gammarus balcanicus, a species complex with a high rate of endemism
Fig. 3. Geographic distribution of the main rare Microsporidia infecting Gammarus balcanicus, showing their occurrence in other gammarid species over Europe. Each map (A–H) refers to the parasite taxa presented in the bottom-right inset. The host and geographic range of the Microsporidia based on this study and 1) literature data: Terry et al. (2004); Wattier et al. (2007); Krebes et al. (2010); Ovcharenko et al. (2010); Bacela-Spychalska et al. (2012); Rode et al. (2013); Grabner et al., 2014; 2015; 2017; Bojko et al. (2015); 2017; 2018; Weigand et al. (2016); Quiles et al. (2019); 2) Gen Bank sequences: MT645708 (Chen,Y. and Jiang, H. direct submission); KP699690 (Bacela-Spychalska, K. direct submission) and 3) Bacela and Ovcharenko, unpublished data.
Fig. 2 in PREVALENCE OF NOSEMA CERANAE (MICROSPORIDIA) IN THE APIS MELLIFERA MELLIFERA BEE COLONIES FROM LONG TIME ISOLATED APIARIES OF SIBERIA
Fig. 2. Distribution of Nosema species in apiaries throughout the Krasnoyarsk Krai (dots
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