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12 results for “Mullus surmuletus”
Macroscopic, histological and stereological image dataset of the Striped red mullet (Mullus surmuletus) ovaries from the English Channel (ICES area 27.7.d) stock
<p><strong>Contents: </strong></p> <p>This dataset can be completed with the : <strong>Macroscopic, histological and stereological image dataset of the Striped red mullet (<em>Mullus surmuletus</em>) ovaries from the Bay of Biscay (ICES area 27.7.g,j & 27.8.a-c) stock</strong>, which can also be found on the Zenodo repository.</p> <p>This dataset contains the macroscopic and histological images of the ovaries of 214 Striped red mullet (female, <em>Mullus surmuletus</em>, Linnaeus 1758) collected from the English Channel stock (ICES area 27.7.d) in February 2021 (n=20), March 2021 (n=13), April 2021 (n=12), May 2021 (n=15), August 2021 (n=15), September 2021 (n=15), October 2021 (n=41), November 2021 (n=10), December 2021 (n=14), January 2022 (n=30), February 2022 (n=15) and August 2022 (n=14).</p> <p> </p> <p><strong>Images:</strong></p> <ul> <li><strong>Macroscopic_pictures.zip: </strong>archive in zip format of 621 pictures (.JPG; 2Mo-8Mo; JPG; 350pp) from 211 female Striped red mullets dissected during this study. Each photo was taken with a digital camera (no flash). For each individual, up to three pictures were taken when possible (Le Meleder <em>et al.</em>, 2022) with : <ul> <li>one picture of the entire fish with its abdominal cavity open with the ovaries in view</li> <li>one picture of the whole fish with the ovaries outside of the abdominal cavity</li> <li>one picture of the ovaries</li> <li>the name of the picture is the same as the fish’s ID number.</li> </ul> </li> </ul> <ul> <li><strong>Histology_slides.zip :</strong> archive in zip format containing the ovarian histological slides digitized using an Olympus V120 slide scanner, x20 lens. The pictures (.vsi from the OlympusVSI format) are of the 484 histological slides acquired during this study.</li> <li>Data was split for smaller size downloads : <ul> <li><strong>Histology_slides_1of5 :</strong> histological sections for individuals numbered 001 to 045</li> <li><strong>Histology_slides_2of5 :</strong> histological sections for individuals numbered 046 to 138</li> <li><strong>Histology_slides_3of5 :</strong> histological sections for individuals numbered 154 to 180</li> <li><strong>Histology_slides_4of5 :</strong> histological sections for individuals numbered 196 to 270</li> <li><strong>Histology_slides_5of5 :</strong> histological sections for individuals numbered 271 to 334</li> </ul> </li> </ul> <p> </p> <p><strong>Data:</strong></p> <ul> <li><strong>Readings.zip :</strong> archive in zip format containing the stereology reading results of the ovarian histological slides. In this folder, three directories are available. <ul> <li><strong>Calibration</strong> : Reading results of 4 different agents, with the first and last readings, as well as the Qupath scripts used.</li> <li><strong>Homogeneity</strong> : Reading results for 96 histological slides used to check the cellular homogeneity inter- and intra-gonad. These 96 slides belong to 16 fish, with three histological samples taken in the anterior (1), median (2) and posterior (3) sections of the left (G) and right (D) ovaries. A QuPath folder is also present, containing the scripts used.</li> <li><strong>Total </strong>: Reading results for 214 ovarian histological slides of the median position of either the left or right ovary. One median slide was read per sampled fish. A QuPath folder is also present, containing the scripts used.</li> </ul> </li> </ul> <ul> <li><strong>Macro_MULL_read_me.txt</strong> : a text file (.txt) listing the acronyms used in the <strong>Macro_MULL.xlsx</strong> file, as well as their meaning.</li> <li><strong>Macro_MULL.xlsx</strong> : Excel file (.xlsx) containing measurements of macroscopic parameters for all 214 fish sampled during this study. The information contained in this table is as follows: <ul> <li>Fish_id: identification of the fish. This id is identical to the name given to the pictures of the full ovaries (<strong>Macroscopic_pictures_Data</strong>)</li> <li>ICES _Division: International Council for the Exploration of the Sea (ICES) division where the fish was sampled in the Food and agricultural Organization of the United nations (FAO) fishing area 27</li> <li>ICES_statistical_rectangle : Statistical rectangle where the fish was sampled within the FAO fishing area 27</li> <li>Date: date the fish was caught (dd/mm/yyyy)</li> <li>Total_fish_length: total length of the fish (cm)</li> <li>Ungutted_fish_weight: total weight of the fish (g)</li> <li>Otolith_ID: unique identification number given to each sampled fish through the Imagine (Ellebode <em>et al.</em>, 2022) software used by IFREMER</li> <li>Parasite: presence (Y) or absence (N) of parasite in or on the fish</li> <li>age: age (in years) of the fish after analysis of the fish’s otolith. The IFREMER laboratory of Boulogne-sur-Mer (FRANCE) executed this analysis</li> <li>Visual_maturity : visually estimated maturity, after observation macroscopic criteria of the fish’s gonad with the naked eye, following the WKASMSF (ICES, 2018) scale</li> <li>Liver_weight: liver weight (g)</li> <li>Droite_gonad_weight : gonad weight (g) of right ovary</li> <li>Gauche_gonad_weight : gonad weight (g) of left ovary</li> <li>Sections: number of cross sections sampled for the individual</li> </ul> </li> </ul> <ul> <li><strong>Stereo_MULL_read_me.txt</strong> : a text file (.txt) listing the acronyms used in the <strong>Stereo_MULL.csv</strong> file, as well as their meaning.</li> <li><strong>Stereo_MULL.csv</strong> : a text data file (.csv) of the stereology count results of 294 slides read during this study. Among these slides, 96 were read to test the homogeneity distribution of different cell types found throughout each ovary (16 fish with 6 histological sections : a median, an anterior and a posterior histological section, for both ovaries), slides were read by multiple agents for calibration purposes (see <strong>Calibration</strong> folder for reading results of the 4 agents). Finally, 214 median histological ovarian slides were read. The information contained in this table is as follows: <ul> <li>cell_type: structure identified for one sample point (for the abbreviations, see Heude-Berthelin <em>et al.</em> 2023)</li> <li>idpt: identification number of the sampling point</li> <li>id: unique complex identification number of the sampling point generated by combining the x and y coordinates</li> <li>x: x coordinate of the sampling point</li> <li>y: y coordinate of the sampling point</li> <li>reading: Indicates if the reading data was used to test cellular homogeneity (Homogeneity) or to the sexual maturity phase</li> <li>slideid: identification number of the digitized histological slide that was used for the stereological count. Shares the same 12 first characters with <strong>Fish_id</strong></li> </ul> </li> </ul>
Macroscopic, histological and stereological image dataset of the Striped red mullet (Mullus surmuletus) ovaries from the Bay of Biscay (ICES area 27.7.g,j & 27.8.a-c) stock
<p><strong>Contents: </strong></p> <p>This dataset can be completed with the : <strong>Macroscopic, histological and stereological image dataset of the Striped red mullet (<em>Mullus surmuletus</em>) ovaries from the English Channel (ICES area 27.7.d) stock</strong>, which can also be found on the Zenodo repository.</p> <p>This dataset contains the macroscopic and histological images of the ovaries of 103 Striped red mullet (female, <em>Mullus surmuletus</em>, Linnaeus 1758) collected from the Bay of Biscay stock (ICES areas 27.7.j,g & 27.8.a-c) in November 2020 (n=9), May 2021 (n=11), June 2021(n=7), July 2021 (n=15), September (n=15), October 2021 (n=3), November 2021 (n=27) and February 2022 (n=15).</p> <p> </p> <p><strong>Images:</strong></p> <ul> <li><strong>Macroscopic_pictures.zip: </strong>archive in zip format of 290 pictures (.JPG; 2Mo-8Mo; JPG; 350pp) from 103 female Striped red mullets dissected during this study. Each photo was taken with a digital camera (no flash). For each individual, up to three pictures were taken when possible (Le Meleder <em>et al.</em>, 2022) with : <ul> <li>one picture of the entire fish with its abdominal cavity open with the ovaries in view</li> <li>one picture of the whole fish with the ovaries outside of the abdominal cavity</li> <li>one picture of the ovaries</li> <li>the name of the picture is the same as the fish’s ID number.</li> </ul> </li> </ul> <ul> <li><strong>Histology_slides.zip:</strong> archive in zip format containing the ovarian histological slides digitized using an Olympus V120 slide scanner, x20 lens. The pictures (.vsi from the OlympusVSI format) are of the 264 histological slides acquired during this study. Data was split for smaller size downloads : <ul> <li><strong>Histology_slides_1of3 :</strong> histological sections for individuals numbered 062 to 094</li> <li><strong>Histology_slides_2of3 :</strong> histological sections for individuals numbered 100 to 250</li> <li><strong>Histology_slides_3of3 :</strong> histological sections for individuals numbered 290 to 304</li> </ul> </li> </ul> <p> </p> <p><strong>Data:</strong></p> <ul> <li><strong>Readings.zip:</strong> archive in zip format containing the stereology reading results of the ovarian histological slides. In this folder, three directories are available. <ul> <li><strong>Calibration </strong>: Reading results of 4 different agents, with the first and last readings, as well as the Qupath scripts used<strong>.</strong></li> <li><strong>Homogeneity</strong> : Reading results for 84 histological slides used to check the cellular homogeneity inter- and intra-gonad. These 84 slides belong to 14 fish, with three histological samples taken in the anterior (1), median (2) and posterior (3) sections of the left (G) and right (D) ovaries. A QuPath folder is also present, containing the scripts used.</li> <li><strong>Total</strong> : Reading results for 103 ovarian histological slides of the median position of either the left or right ovary. One median slide was read per sampled fish. A QuPath folder is also present, containing the scripts used.</li> </ul> </li> </ul> <ul> <li><strong>Macro_MULL_read_me.txt</strong> : a text file (.txt) listing the acronyms used in the <strong>Macro_MULL.xlsx</strong> file, as well as their meaning.</li> <li><strong>Macro_MULL.xlsx</strong> : Excel file (.xlsx) containing measurements of macroscopic parameters for all 103 fish sampled during this study. The information contained in this table is as follows: <ul> <li>Fish_id: identification of the fish. This id is identical to the name given to the pictures of the full ovaries (<strong>Macroscopic_pictures_Data</strong>)</li> <li>ICES _Division: International Council for the Exploration of the Sea (ICES) division where the fish was sampled in the Food and agricultural Organization of the United nations (FAO) fishing area 27</li> <li>ICES_statistical_rectangle : Statistical rectangle where the fish was sampled within the FAO fishing area 27</li> <li>Date: date the fish was caught (dd/mm/yyyy)</li> <li>Total_fish_length: total length of the fish (cm)</li> <li>Ungutted_fish_weight: total weight of the fish (g)</li> <li>Otolith_ID: unique identification number given to each sampled fish through the Imagine (Ellebode <em>et al.</em>, 2022) software used by IFREMER</li> <li>Parasite: presence (Y) or absence (N) of parasite in or on the fish</li> <li>age: age (in years) of the fish after analysis of the fish’s otolith. The IFREMER laboratory of Boulogne-sur-Mer (FRANCE) executed this analysis</li> <li>Visual_maturity : visually estimated maturity, after observation macroscopic criteria of the fish’s gonad with the naked eye, following the WKASMSF (ICES, 2018) scale</li> <li>Liver_weight: liver weight (g)</li> <li>Droite_gonad_weight : gonad weight (g) of right ovary</li> <li>Gauche_gonad_weight : gonad weight (g) of left ovary</li> <li>Sections: number of cross sections sampled for the individual</li> </ul> </li> </ul> <ul> <li><strong>Stereo_MULL_read_me.txt</strong> : a text file (.txt) listing the acronyms used in the <strong>Stereo_MULL.csv</strong> file, as well as their meaning.</li> <li><strong>Stereo_MULL.csv</strong> : a text data file (.csv) of the stereology count results of 173 slides read during this study. Among these slides, 84 were read to test the homogeneity distribution of different cell types found throughout each ovary (14 fish with 6 histological sections : a median, an anterior and a posterior histological section, for both ovaries), slides were read by multiple agents for calibration purposes (see <strong>Calibration</strong> folder for reading results of the 4 agents). Finally, 103 median histological ovarian slides were read. The information contained in this table is as follows: <ul> <li>cell_type: structure identified for one sample point (for the abbreviations, see Heude-Berthelin <em>et al.</em> 2023)</li> <li>idpt: identification number of the sampling point</li> <li>id: unique complex identification number of the sampling point generated by combining the x and y coordinates</li> <li>x: x coordinate of the sampling point</li> <li>y: y coordinate of the sampling point</li> <li>reading: Indicates if the reading data was used to test cellular homogeneity (Homogeneity) or to the sexual maturity phase</li> <li>slideid: identification number of the digitized histological slide that was used for the stereological count. Shares the same 12 first characters with <strong>Fish_id</strong></li> </ul> </li> </ul>
Figure 1 in Are fisheries regulations influencing the biology and reproduction of the surmullet Mullus surmuletus Linnaeus, 1758 on the south-eastern coasts of France (NW Mediterranean)?
Figure 1. – Sampling sites in West, AMA (Adjacent Marine Protected Area of Hyères Bay) and East zones on the south-eastern coast of France, NW Mediterranean Sea.
Figure 4 in Are fisheries regulations influencing the biology and reproduction of the surmullet Mullus surmuletus Linnaeus, 1758 on the south-eastern coasts of France (NW Mediterranean)?
Figure 4. – Percentages of both sexes and females:males sex-ratio of Mullus surmuletus by zone (A) and season (B).
Figure 3 in Are fisheries regulations influencing the biology and reproduction of the surmullet Mullus surmuletus Linnaeus, 1758 on the south-eastern coasts of France (NW Mediterranean)?
Figure 3. – Mean (± SE) total length (TL, cm) of Mullus surmuletus in West, AMA and East zones. N: number of analyzed individuals per sex in zones. Values with the same post-hoc letters (red for females, blue for males) are not significantly different (p> 0.05).
Figure 2 in Are fisheries regulations influencing the biology and reproduction of the surmullet Mullus surmuletus Linnaeus, 1758 on the south-eastern coasts of France (NW Mediterranean)?
Figure 2. – Percentage of individuals of Mullus surmuletus analyzed by two-cm size class (total length in cm) and by sex. N: number of individuals, F: females, M: males, Undet.: unidentified group includes immature individuals and those whose sex could not be identified.
Figure 8 in Are fisheries regulations influencing the biology and reproduction of the surmullet Mullus surmuletus Linnaeus, 1758 on the south-eastern coasts of France (NW Mediterranean)?
Figure 8. – Mean percentages of gonadal development stages (GDS) of Mullus surmuletus. A: By zone for females; B: By zone for males; C: By season for females; D: By season for males. N: number of individuals.
Figure 6 in Are fisheries regulations influencing the biology and reproduction of the surmullet Mullus surmuletus Linnaeus, 1758 on the south-eastern coasts of France (NW Mediterranean)?
Figure 6. – Mean gonado-somatic index (GSI, %) of males and females of Mullus surmuletus by (A) zone and (B) season. Values with the same post-hoc let-letters (red for females, blue for males) are not significantly different (p> 0.05).
Figure 7 in Are fisheries regulations influencing the biology and reproduction of the surmullet Mullus surmuletus Linnaeus, 1758 on the south-eastern coasts of France (NW Mediterranean)?
Figure 7. – Mean percentage of gonadal development stages of Mullus surmuletus by 2-cm size class (TL, cm) and sex for (A) females and (B) males. N: number of individuals.
Figure 5 in Are fisheries regulations influencing the biology and reproduction of the surmullet Mullus surmuletus Linnaeus, 1758 on the south-eastern coasts of France (NW Mediterranean)?
Figure 5. – Mean gonado-somatic index (GSI, %) of Mullus surmuletus with size (LT, cm) by sex.
Mullus surmuletus environmental DNA intraspecific metabarcoding Next-Generation Sequencing data
<p>Four 250-liter aquariums were bleached clean one day prior to be used (filled with seawater; fish transfer) in Montpellier (France). Seawater collected by the French Research Institute for Exploitation of the Sea at Palavas-les-Flots (France) was first stored in a 1,000 L tank for two weeks, under UV treatment to avoid any contamination. The aquariums were then filled with 120 L of this water. Each aquarium had a closed-circuit water circulation and was equipped with an air bubbles exhauster in a tube that brought up the water on a neutral synthetic foam filter. The aquariums were thus oxygenated and the coarsest suspended matter was filtered out. The remaining seawater in the tank was used as a negative control (Aquarium 1). Nine to eleven fish were added to each of the four aquariums (Fig. 1). The aquarium water was sampled six hours after introducing the fish into the aquariums using an Athena peristaltic pump (SPYGEN, Le Bourget-du-Lac, France) with a nominal flow of 1.0 L/min to filter 30 L, and VigiDNA 0.22 μm crossflow filtration capsules (SPYGEN) with disposable sterile tubing. After filtration, 80 mL of CL1 conservation buffer (SPYGEN) was added before storing the samples at ambient temperature.</p> <p> </p> <p>We reanalyzed here two eDNA samples of 30 L replicate each, collected in the Mediterranean Sea, at Banyuls (France, coordinates: 42.41568, 3.17110) and Calvi (France, coordinates: 42.62964, 8.89161) published in a previous metabarcoding analysis and known to contain <em>M. surmuletus</em> sequences (detected with the metabarcode teleo 12S) (Boulanger <em>et al.</em> 2021). These two Mediterranean eDNA samples were amplified and sequenced using the primers developed for this study and then analyzed using the best-performing pipeline as determined by our evaluation. These two samples were used as proof of concept of the possibility to estimate within site variability in real conditions.</p> <p> </p> <p> </p> <p>DNA extraction and amplification from eDNA samples were performed by the company SPYGEN (Le Bourget du Lac, France) in separate, dedicated rooms following the protocol described by Polanco Fernández <em>et al.</em> (2020). The amplification was performed in a final volume of 25 μL including 1 U of AmpliTaq Gold DNA Polymerase (Applied Biosystems, Foster City, CA, USA), 10 mM of Tris-HCl, 50 mM of KCl, 2.5 mM of MgCl2, 0.2 mM of each dNTP, 0.2 μM of each primer, 0.2 μg/μL of bovine serum albumin (Roche Diagnostics, Basel, Switzerland) and 3 μL of DNA template. The PCR mixture was denatured at 95°C for 10 min, followed by 50 cycles of 30 s at 95°C, 30 s at 47°C and 1 min at 72°C and a final elongation step at 72°C for 7 min. The primers were 5’-labelled with an eight-nucleotide tag unique to each DNA sample, allowing each sequence to be assigned to the corresponding sample during the sequence analysis. Twelve replicate PCRs were run per sample. Two libraries were prepared using the MetaFast protocol (Fasteris 2020, <a href="https://www.fasteris.com/dna/">https://www.fasteris.com/dna/</a>) and the sequencing was performed by Fasteris (Geneva, Switzerland) on two separate runs on an Illumina MiSeq (2x250 bp) (Illumina, San Diego, CA, USA) and the Miseq Kit v3 (Illumina) following the manufacturer’s instructions. Two negative extraction controls and one negative PCR control (12 replicates of ultrapure water) were amplified and sequenced to monitor for possible contaminants (Polanco Fernández <em>et al.</em>, 2020).</p> <p> </p> <p> </p> <p> </p>
FIGURE 63. Mullus surmuletus Linnaeus 1758 in The marine ichthyofauna of Lebanon: an annotated checklist, history, biogeography, and conservation status
FIGURE 63. Mullus surmuletus Linnaeus 1758, Tripoli, 15 July 2011.
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