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10 results for “Multiphoton microscopy”
Insights into metabolic changes during epidermal differentiation as revealed by multiphoton microscopy with fluorescence lifetime imaging
<p>Rapid developments in the field of organotypic cultures has generated a growing need for effective quality control measures during tissue development. In this study, we correlate metabolic changes with epidermal differentiation and demonstrate that multiphoton microscopy with fluorescence lifetime imaging (MPM-FLIM) can be applied as a non-invasive approach to monitor epidermal differentiation of keratinocytes with respect to proliferative and differentiated states. Keratinocytes grown at 1.5 mM Ca2+ exhibited increased expression of differentiation markers KRT1 and KRT10 compared to 60 μM Ca2+, and a metabolic shift from glycolysis to mitochondrial respiration. Fitting the fluorescence decay with a biexponential model revealed a decreased relative fraction of intracellular NADH and FAD after high calcium treatment, consistent with increased oxidative phosphorylation. Using these two parameters, the epidermal differentiation process could be monitored over a 96 h period. Implementing discriminating analysis based on k-means clustering generated clusters that correlated well with culturing time, suggesting that this methodology can be employed as part of an automated pipeline for monitoring keratinocyte differentiation.</p>
Raw data for: Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre
<p>Raw data files for publication "Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre" in Journal of Biophotonics 2016</p>
Novel Pixelwise Co-Registered Hematoxylin-Eosin and Multiphoton Microscopy Image Dataset for Human Colon Lesion Diagnosis
<p><strong>General Description:</strong></p> <p>The dataset consists of a set of 50 samples of lesions obtained by colonoscopies and colectomies carried out between the years 2012 and 2017 at at Digestive Service at OSI Bilbao Basurto. These are 24 malignant neoplasms (adenocarcinoma), 19 preneoplastic lesions (adenoma) and 2 hyperplasia and 5 healthy tissues, obtained from 24 men and 19 women. The samples were diagnosed by the Pathological Anatomy Department at OSI Bilbao Basurto and the FFPE (Formalin-Fixed Paraffin-Embedded) blocks were stored in the Basque Biobank. All the samples were processed after signing Informed Consent and following standard operation procedures. The samples were scanned using a multiphoton microscope (Lens, Florence, Italy) both for multi-photon fluorescence (MPM) and second harmonic generation (SHG) and later stained with H&E (Hematoxylin & Eosin).</p> <p>The different image modalities were reconstructed and corregistered by performing non-rigid deformation (Tecnalia, Bilbao, Spain) allowing pixel correspondence among the different modalities. Pathologists from Basurto Hospital manually labeled the regions where the lession is present. Scale of current dataset is 0.5um/px.</p> <p><strong>Technical Details:</strong></p> <p>On the data_info.csv information for each sample is included:<br> - SAMPLE_ID: Includes the tag "PICCOLO_XX_YY", where XX stands for the patient ID number and YY to the lesion suffix ID.<br> - LESION_ID: The lesion is classified as healthy, hyperplasia, benign neoplasia, and malign neoplasia.<br> - HISTOLOGICAL ANALYSIS: It includes informative test about the lesion.<br> - GRADE: In the case for malign neoplasia universal grading system grade is included.</p>
Demo data for working with Learned Adaptive Multiphoton Illumination microscopy notebook
<p>This data is used for running parts of the Jupyter notebook describing Learned Adaptive Multiphoton Illumination microscopy in Pycro-Manager/Micro-Magellan in demo mode</p>
Evaluating Light-tissue Reaction and Transdermal Drug Delivery by Multiphoton Microscopy
ClinicalTrials.gov study NCT01355328. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Use of Multiphoton Microscopy in the Diagnosis of Cancer
ClinicalTrials.gov study NCT00652210. IPD Sharing: Not stated. Countries: 1. Publications: 0.
A Pilot Study to Evaluate Multi-Spectral and Laser Speckle Imaging and Multiphoton Microscopy During Vascular Occlusion
ClinicalTrials.gov study NCT01484730. IPD Sharing: NO. Countries: 1. Publications: 0.
Multiphoton Laser Scanning Microscopy in Cutaneous Optical Pathology
ClinicalTrials.gov study NCT00508781. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Pilot Study on In-vivo Non-invasive Skin Imaging Using Multiphoton Microscopy
ClinicalTrials.gov study NCT01771718. IPD Sharing: NO. Countries: 1. Publications: 0.
Alteration of Dermal Elastic Fibers During Calcifying Dermatosis: Structural Study Using Multiphoton Microscopy
ClinicalTrials.gov study NCT05112744. IPD Sharing: NO. Countries: 1. Publications: 0.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.