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3 results for “ND2 gene”
DNA sequence data generated using non-invasive feather and eggshell samples from the Grenada Dove for two gene regions: Cyt b and ND2
<p>As an island endemic with a decreasing population, the Critically Endangered Grenada Dove <em>Leptotila wellsi</em> is threatened by accelerated loss of genetic diversity resulting from ongoing habitat fragmentation. Small, threatened populations are difficult to sample directly but advances in molecular methods mean that non-invasive samples can be used. We performed the first assessment of genetic diversity of populations of Grenada Dove by a) assessing mtDNA genetic diversity in the only two areas of occupancy on Grenada, b) defining the number of haplotypes present at each site and c) evaluating evidence of isolation between sites. We used non-invasively collected samples from two locations: Mt Hartman (n=18) and Perseverance (n=12). DNA extraction and PCR were used to amplify 1,751 bps of mtDNA from two mitochondrial markers: NADH dehydrogenase 2 (<em>ND2</em>) and Cytochrome b (<em>Cyt b</em>). Haplotype diversity (<em>h</em>) of 0.4, a nucleotide diversity (π) of 0.00023 and two unique haplotypes were identified within the <em>ND2</em> sequences; a single haplotype was identified within the <em>Cyt b </em>sequences. Of the two haplotypes identified; the most common haplotype (haplotype A = 73.9%) was observed at both sites and the other (haplotype B = 26.1%) was unique to Perseverance. Our results show low mitochondrial genetic diversity and clear evidence for genetically isolated populations. The Grenada Dove needs urgent conservation action, including habitat protection and potential augmentation of gene flow by translocation in order to increase genetic resilience and diversity with the ultimate aim of securing the long-term survival of this Critically Endangered species. </p>
Partial NADH dehydrogenase subunit 2 (ND2) gene sequences for Trochilidae hummingbirds
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DNA sequence data generated using non-invasive feather and eggshell samples from the Grenada Dove for two gene regions: Cyt b and ND2
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