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12 results for “Ngan”
FIGURE 1 in Preface-A carcinological tribute to Ng Ngan Kee (14 April 1966 to 5 July 2022)
FIGURE 1. Ng Ngan Kee in her prime. (Left) Conducting a workshop at the open house event of Raffles Museum of Biodiversity Research (now Lee Kong Chian Natural History Museum) in 2012; (Right) Study trip to India in September 2011. Photographs: (Left) Joelle C.Y. Lai; (Right) Bee Yan Lee.
FIGURE 8 in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 8. Gecarcinucid sp., juvenile female, 7.7 × 9.9 mm, Lubang Tebot Cave, Merabu Karst, East Kalimantan, Borneo, 1°34'58"N, 117°21'07"E, 360 m, 09 August 2010, MZB Cru 5712: A, habitus; B, frontal view; C, cheliped; D, ventral view; E, thoracic sternum. Scale bars: A, B, D, E = 5 mm; C = 1 mm.
FIGURE 7. A in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 7. A, Isolapotamon sp., Lubang Bayan Cave, Merabu Karst, East Kalimantan, Borneo, 01°28'56"N, 117°11'32"E, August 2010, photographed in situ in the river preying on barb; crab not collected, probably a trogloxene; B, Isolapotamon sp., juvenile female, 7.8 × 8.9 mm, Lubang Tebot Cave, Kalimantan (East Kalimantan), Borneo, 1°34'58"N, 117°21'07"E, 360 m, 09 August 2010, MZB Cru 5711. Scale bar: B = 5 mm.
FIGURE 3 in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 3. Ngan mayla gen. et sp. nov., holotype female, 12.2 × 15.3 mm, MZB Cru 5710: A, frontal view; B, left cheliped; C, D, carapace and chelipeds; E, ventral view; F, ventral anterior view. Scale bars: A–E = 5 mm; F = 1 mm.
FIGURE 6 in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 6. Vulvae: A, Ngan mayla gen. et sp. nov., holotype female, 12.2 × 15.3 mm, MZB Cru 5710; B, Cerberusa caeca Holthuis, 1979, female, 9.9 × 13.3 mm, ZRC 2013.1220; C, C. tipula Holthuis, 1979, female, 13.0 × 17.6 mm, ZRC 2013.1219; D, Ibanum aethes Ng, 1995, female, 10.8 × 14.7 mm, ZRC 2019.0141. B–D, courtesy P.K.L. Ng.
FIGURE 2 in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 2. Ngan mayla gen. et sp. nov., holotype female, 12.2 × 15.3 mm, Kalimantan (East Kalimantan), Indonesia, Borneo, Merabu Karst, Lubang Gedung Cave, 1°38'1"N–117°24'14"E, 850 m, 18 August 2010, MZB Cru 5710: habitus. Scale bar = 5 mm.
FIGURE 1. A in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 1. A, Borneo Island and Kalimantan; B, Karst of Merabu, East Kalimantan, northernmost limestone massif of the Mangkalihat Peninsula; C, D, Lubang Gedung Cave, two views. Courtesy, J. and B. Lips.
FIGURE 5. Cerberusa tipula Holthuis, 1979 in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 5. Cerberusa tipula Holthuis, 1979, female, 13.0 × 17.6 mm, Borneo, Sarawak, Stone Horse Cave, ZRC 2013.1219: A, habitus; B, frontal view; C, left cheliped; D, carapace and chelipeds; E, ventral view. Courtesy P.K.L. Ng.
FIGURE 4. Cerberusa caeca Holthuis, 1979 in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 4. Cerberusa caeca Holthuis, 1979, female, 9.9 × 13.3 mm, Borneo, Sarawak, Stone Horse Cave, ZRC 2013.1220: A, habitus; B, D, frontal views; C, left cheliped; E, ventral view. Courtesy, P.K.L. Ng.
FIGURE 9 in Ngan mayla gen. et sp. nov, a new blind potamid cave crab from Borneo, Kalimantan (Crustacea: Decapoda: Brachyura: Potamoidea, Potamidae), and three other unidentified cave crabs from the same region
FIGURE 9. Ocular regression: A, Ngan mayla gen. et sp. nov., (Potamidae), holotype female, 12.2 × 15.3 mm, MZB Cru 5710; B, Cerberusa caeca Holthuis, 1979 (Potamidae), male, 12.5 × 17.0 mm, RMNH.CRUS.D.31904; C, C. tipula Holthuis, 1979 (Potamidae), male 12.5 × 16.0 mm, female 16.0 × 21.8 mm, RMNH.CRUS.D.33959; D, Holthuisana alba Holthuis, 1980 (Gecarcinucidae), paratype female, 22.0 × 27.0 mm, RMNH.CRUS.D.33302; E, Sendleria genuitei Guinot, 1987 (Gecarcinucidae), holotype male, 22.0 × 29.0 mm, MNHN-IU-2014-11301 (= MNHN-B17968); F, Typhlopseudothelphusa juberthiei Delamare Deboutteville, 1976 (Pseudothelphusidae Ortmann, 1893), female, 21.0 × 32.0 mm, MNHN-IU-2014- 23194 (= MNHN-B18870); G, Trogloplax joliveti Guinot, 1986 (Trogloplacinae Guinot, 1986), paratype male, 16.0 × 21.0 mm, MNHN-IU-2014-10458 (= MNHN-B12794); H, Austinograea alayseae Guinot, 1990 (Bythograeoidea Williams, 1980), allotype female, 21.0 × 32.0 mm, MNHN-IU-2008-11306 (= MNHN-B24022). Scale bar: A = 1 mm.
An environmental DNA metabarcoding approach versus a visual survey for reefs of Koh Pha‐ngan in Thailand
<p><span>Information on diversity indices and abundance of individual species is crucial for the assessment of ecosystem health, especially for endangered ecosystems such as coral reefs. The application of environmental DNA (eDNA) to monitor coral biodiversity is, however, just beginning to come into focus for marine biologists. In this study, an eDNA metabarcoding approach of seawater samples in three different reefs on Koh Pha-ngan, Thailand, was compared with simultaneously collected visual census data. In addition, differences in read abundance and number of genera detected between daytime and nighttime eDNA samples were examined, and a local coral barcode reference database (n=23 genera; COI gene) was constructed to improve assignment of eDNA reads to the genus level. As a technical extension of existing assays, two methods for library construction were compared: a commercial kit and in-house developed fusion primers.</span></p> <p><span>Combining eDNA metabarcoding and visual data, 29 different genera of scleractinian corals from 14 families were detected. In addition, a log-linear correlation was found between the abundance of eDNA reads and visually determined relative coral cover at the genus level, suggesting a predictive relationship between eDNA reads and coral cover. Results also showed diurnal variation between day and night samples in the number of eDNA reads, purported to relate to the activity phases of corals. The use of uniquely labeled fusion primers gave comparable results to a commercially available library preparation kit. Especially with frequent use, fusion primers can be very cost-effective, and therefore a consideration for large-scale studies. Using a custom reference database of 89 sequences from coral tissue samples of 23 different coral genera produced better results than querying against NCBI GenBank, highlighting the importance of locally optimized databases. We consider these results important for establishing eDNA as a complementary tool to visual surveys to track changes in coral diversity and cover.</span></p>
An environmental DNA metabarcoding approach versus a visual survey for reefs of Koh Pha‐ngan in Thailand
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