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39 results for “Nitrogen stable isotopes”
Carbon and nitrogen content and stable isotope compositions from biota samples from lagoon sites along the Alaska Beaufort Sea coast, 2018-ongoing
Stable isotopic composition can be used to differentiate between predominantly marine and terrestrial food sources in nearshore marine food webs. The Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program employs spatial and temporal sampling regimes to track shifts in diet, both seasonally (ice cover, ice break-up, and open water), and spatially among lagoons along the Alaskan Beaufort Sea coast. Ice coring, net tows, ponar grabs, and trawls are employed to collect organisms associated with the sea-ice interface, the water column, and the benthos respectively. Tissues are analyzed for stable isotopic composition as well as organic carbon and nitrogen content.
Carbon and nitrogen content and stable isotope compositions from particulate organic matter samples from lagoon, river, and open ocean sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, and analyzed for particulate organic carbon (POC) and particulate organic nitrogen (PON) content and stable isotopic composition.
Carbon and nitrogen content and stable isotope composition from sediment organic matter from lagoon sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple sediment samples from lagoons along the nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Sediment samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, and analyzed for carbon and nitrogen content and stable isotopic composition.
Primary producer biomarker profiles of bulk carbon and nitrogen stable isotopes (SI), compound specific stable isotopes of carbon in essential amino acids (CSIA-EAA) and their fatty acid (FA) collected from the Beaufort Sea coastal lagoons,2021-2024
Within Stefansson Sound in Prudhoe Bay, AK various organic matter sources were collected to determine multiple biomarker baseline profiles (i.e., bulk carbon and nitrogen stable isotopes (SI), compound specific stable isotopes of carbon in essential amino acids (CSIA-EAA), fatty acids (FA)). Some organic matter sources were collected from Elson lagoon in Utqiaġvik, AK and Kaktovik and Jago lagoons in Kaktovik, AK to supplement low sample sizes in some organic matter source groups. Kelp, red algae, terrestrial plants, phytoplankton, and ice algae were collected in 2024 with some supplement samples collected in 2021 - 2023. Stable isotope values of δ13C and δ15N are reported as “del_13c” and “del_15n”, respectively. Individual fatty acids are reported as the percent relative to total fatty acids for 23 fatty acids: C11:0, C12:0, C14:0, C15:1, C15:0, C16:0, C16:1n7, C17:0, C17:1, C18:0, C18:1n9 trans, C18:2n6 cis, C18:1n7, C18:3n3, C20:0, C18:3n6, C20:4n6, C21:0, C22:0, C22:1n9, C23:0, C24:0, C22:6n3. Stable isotope values of δ13C are reported in the following essential amino acids: Valine (Val), Leucine (Leu), iLeu (isoleucine), Methionine (Met), Phenylalanine (Phe). Additionally, we used ice algal diatoms collected in the Arctic (landfast ice near Utqiaġvik, Alaska) and cultured in a laboratory setting at the University of Alaska Fairbanks to compare the CSIA-EAA fingerprints of field (composites) ice algal samples and isolate diatoms samples.
Soil and foliar carbon and nitrogen content and stable isotope ratios from rainfall manipulation experiments at the Jornada Basin LTER, 2011-2020
As rainfall extremes are expected to increase in novel magnitude and frequency, especially in dryland regions, we asked how prolonged and directional shifts to water availability may affect ecosystem carbon and nitrogen dynamics. This data set includes foliar and soil carbon and nitrogen stable isotope and concentration data collected from multiple long-term rainfall manipulation experiments at the Jornada Basin LTER. Datasets also include rainfall data adjusted to rainfall manipulation intensities. Collection dates range from 5 to 14 years since the onset of experimental treatments. The primary plant species targeted for this study were the dominant grass, Bouteloua eriopoda, and the dominant shrub, Prosopis glandulosa.
Particulate organic carbon and particulate organic nitrogen concentrations and stable isotope composition of seawater sampled during the Antarctic Circumnavigation Expedition (ACE) during the Austral Summer of 2016/2017.
<p><strong>Dataset abstract</strong></p> <p>This dataset contains particulate organic carbon and particulate organic nitrogen concentrations and stable isotope composition (delta 13C and delta 15N) sampled during the Antarctic Circumnavigation Expedition (ACE) Leg 1-3. Water samples were collected from the underway seawater supply every 3 hours, filtered onto pre-combusted glass fibre filters, acidified to remove inorganic compounds and analysed for both elements on the same filter using an elemental analyser. These samples provide an estimate of the organic carbon and organic nitrogen concentration and carbon and nitrogen stable isotope composition of living and detrital particles > 0.7 micrometres in size.</p> <p><strong>Dataset contents</strong></p> <ul> <li>README.txt, metatdata, text</li> <li>data_file_header.txt, metadata, text</li> <li>ace_uw_poc_pon_blanks_20200512CURRSGCMR.csv, data file, comma-separated values</li> <li>ace_uw_poc_pon_20200512CURRSGCMR.csv, data file, comma-separated values</li> </ul>
Stable carbon and nitrogen isotope data from Arctic coastscapes associated with coastal invertebrate and fish food webs, 1999-2022
Stable carbon and nitrogen isotope data are commonly used to elucidate food web structure and partition food source importance to consumers. Here, stable isotope data were gathered from across the coastal Arctic to assess how differences in coastal type (i.e., coastscape) and longitudinal region differ across the Arctic. Data were collected between 1999-2022.
Mass per tiller, nitrogen concentration, stable isotope ratios for carbon and nitrogen from the 1980-82 Eriophorum vaginatum reciprocal transplant experiment along a latitudinal gradient in interior Alaska collected in July, 2011
In 1980-1982, six transplant gardens were established along a latitudinal gradient in interior Alaska from Eagle Creek, AK in the south to Prudhoe Bay, AK in the north. Three sites, Toolik Lake (TL), Sagwon (SAG), and Prudhoe Bay (PB) are north of the continental divide and the remaining three, Eagle Creek (EC), No Name Creek (NN), and Coldfoot (CF), are south of the continental divide. Each garden consisted of 10 individual Eriophorum vaginatum tussocks transplanted back to their home-site, as well as 10 individuals from each of the other transplant sites. The gardens were harvested in 2011. Important variables are garden name, source population, mass per tiller, nitrogen concentration, and stable isotope ratios for Carbon and Nitrogen.
SIA-BRA: The carbon and nitrogen stable isotope ratios of animals of Brazilian biomes and coastal marine areas
<p>SIA-BRA is a compilation of C and N stable isotope ratios of terrestrial and aquatic animals sampled in Brazilian biomes and coastal-marine areas.</p> <p>Version 1.0 contains isotopic data of c. 21,804 non-captive wildlife specimens, excluding livestock production or laboratory<br> experiments. They were 13,881 vertebrates and 7,923 invertebrates. There are 11 phyla, with a clear dominance of Chordata (64%) and Arthropoda (29%), 36 classes, 154 orders, 473 families, 894 genera and 1,157 species.</p> <p>They were divided into the following habitats: terrestrial (30% of the total), freshwater (27%), oceanic (40%)<br> and estuarine (4%) (see <a href="https://doi.org/10.1111/geb.13449">https://doi.org/10.1111/geb.13449</a>)</p> <p>Software format: Data are supplied as delimited text files (.csv).</p>
Nitrogen and carbon concentrations and stable isotope ratios (δ¹⁵N and δ¹³C) in European moss samples, 2005-2006
This dataset contains nitrogen (N) and carbon (C) concentrations and stable isotope ratios (δ¹⁵N and δ¹³C) measured in moss samples collected across Europe within the framework of the ICP Vegetation programme (International Cooperative Programme on Effects of Air Pollution on Natural Vegetation and Crops, UNECE LRTAP Convention). Moss surveys are conducted every five years and the data presented here correspond specifically to the first sampling campaign, carried out in 2005/2006. During the 2005/2006 European moss survey, approximately 3,000 moss samples were collected at non-urban and semi-natural sites across 16 European countries following a standardized biomonitoring protocol. The dataset used in this study comprises a subset of 1,022 moss samples (approximately 35 % of the total survey), provided by 12 European countries, which were selected for the determination of nitrogen and carbon concentrations and their corresponding stable isotope signatures (δ¹⁵N and δ¹³C). Moss samples collected by each participating country were sent to the Integrated Environmental Quality Laboratory (LICA), Institute for Biodiversity and Environment (BIOMA - University of Navarra), where all chemical and isotopic analyses were subsequently performed under uniform analytical conditions. In addition, this dataset incorporates moss data from Sweden, Croatia and Macedonia for the same sampling year, which were not included in the official ICP Vegetation 2005/2006 dataset. The European moss biomonitoring network was established to provide a complementary, high spatial resolution and time-integrated measure of atmospheric deposition of nitrogen and other pollutants within terrestrial ecosystems. The approach is based on the ability of ectohydric mosses to accumulate nutrients and trace elements directly from wet and dry atmospheric deposition, enabling dense spatial sampling across large geographical areas. This biomonitoring framework supports the assessment of spatial patterns of atmos
PIE LTER, stable isotope chemistry (carbon, nitrogen and sulfur) for food web analysis of functional groups in the Plum Island Sound Estuary, Massachusetts.
Stable isotopes of primary producers will be compared to stable isotopes of functional groups of organisms at primarily three sites within the estuary that have different dominant sources of organic matter. The three sites are: Lower (IBYC, SO-3, mouth of Plum Island Sound, 2-3 km upstream of the mouth of the estuary), Middle (OTL, PR-10.1, upper Sound, lower Parker, 8-11 km upstream of the mouth of the estuary) and Upper (P2, PR-21.9, upper Parker, above Middle Rd Bridge (22 km upstream of the mouth of the estuary). The Lower site is dominated by marine phytoplankton, the Middle site is dominated by a mixture of salt marsh and phytoplankton and the Upper site is dominated by oligohaline phytoplankton and fresh marsh. Ten functional groups will be sampled at each site (Surface sediment, benthic diatoms, Nereis, mummichog, ribbed mussels, POM, blue mussels, pelagic copepods (Acartia), silversides and soft shell clams (Mya). Marsh, benthic algae and phytoplankton inputs or benthic vs. pelagic pathways will be evident in the isotopic signals of these functional groups. Samples will be collected between the middle and end of August to reflect a growing season using recently produced OM. Samples of 15 – 20 individuals will be pooled for analysis. Some silverside samplings will have 3 different pooled samples for determination of variance. Often times the same species are not collected at each site due to habitat differences (salinity/discharge) so additional species are collected to try to accomplish task of getting functional groups collected.
Stable isotope content (carbon, nitrogen) for epibenthic suspension feeders and Macrocyctis pyrifera at in the Santa Barbara Channel, 2010-2011
This dataset includes measurments of stable carbon, nitrogen isotopes in tissues of eight epibenthic suspension feeders and Macrocystis pyrifera (giant kelp) at three reefs in the Santa Barbara Channel during 2010 and 2011, as part of a study to (a) determine the contributions of phytoplankton and giant kelp detritus to the pool of suspended reef particulate organic matter (POM), and usage by benthic susupension feeders and (b) whether POM composition varies with distance from kelp forests. Species sampled include Chaceia ovoidea (bivalve), Aglaophenia spp (ostrich plume hydrozoan), Cucumaria piperata (echinoderm), Muricea californica (cnidarian), Phragmatopoma californica (tube worm), Parapholas californica (bivalve), Pachythyone rubra (echinoderm), and Styela montereyensis (tunicate). Samples were collected at three core SBC LTER research reefs: Arroyo Quemado, Arroyo Burro, and Mohawk. Potentially important food sources to benthic suspension-feeders on shallow subtidal reefs include phytoplankton-dominated seston, kelp-derived detritus, and for locations adjacent to sources of freshwater runoff, terrestrially-derived material. Our data will be input to mixing models to estimate the contribution of each source to the reef food web.
Stable isotope (carbon, nitrogen and sulfur) data for primary producers and consumer organisms in the Plum Island Sound Estuary.
Flora and fauna stable isotope study to help characterize organic matter/primary production sources important to the food web of the Plum Island Sound estuary. Sampling occured during 1993 and 1994.
Carbon and nitrogen stable isotope values for lake trout from 6 different Arctic lakes near Toolik, Arctic LTER 1987 to 1988.
Lake trout were analysed for carbon and nitrogen stable isotope values in 6 Arctic lakes near Toolik Lake at the Arctic LTER in 1987 and 1988. The fish were also analysed for age using otoliths.
Nitrogen and oxygen stable isotopes of nitrate from CalCOFI lines 80 and 93 plus station 081.8 046.9. Collected on cruises from 2010 to 2016.
Stable isotopes of nitrate were measured on CalCOFI cruises from 2010-2016 from lines 80 and 93 plus station 081.8 046.9. Samples were filtered directly from the CTD and then frozen until analysis. Samples collected from 2010-2012 were analyzed in the Sigman Lab at Princeton University. Samples collected from 2013-2016 were analyzed in the Wankel Lab at Woods Hole Oceanographic Insititution. Five samples from station 081.8 046.9 (the Santa Barbara Basin) were further analyzed for nitrogen isotopes of nitrite in the Wankel Lab.
Quality control for modern bone collagen stable carbon and nitrogen isotope measurements
<p><strong>(1)</strong> Isotopic analyses of collagen, the main protein preserved in sub fossil bone and tooth, has long provided a powerful tool for the reconstruction of ancient diets and environments. Although isotopic studies of contemporary ecosystems have typically focused on more accessible tissues (e.g., muscle, hair), there is growing interest in the potential for analyses of collagen because it is often available in hard tissue archives (e.g., scales, skin, bone, tooth), allowing for enhanced long-term retrospective studies. The quality of measurements of the stable carbon and nitrogen isotopic compositions of ancient samples are subject to robust and well-established criteria for detection of contaminants and digenesis. Among these quality control (QC) criteria, the most widely utilized is the atomic C:N ratio (C:N<sub>Atomic</sub>), which for ancient samples has an acceptable range between 2.9 and 3.6. While this QC criterion was developed for ancient materials, it has increasingly being applied to collagen from modern tissues.</p> <p><strong>(2)</strong> Here we use a large survey of published collagen amino acid compositions (n<em> </em>= 436) from 193 vertebrate species as well as recent experimental isotopic evidence from 413 modern collagen extracts to demonstrate that the C:N<sub>Atomic</sub> range used for ancient samples is not suitable for assessing collagen quality of modern and archived historical samples.</p> <p><strong>(3) </strong>For modern tissues, collagen C:N<sub>Atomic</sub> falling outside 3.00–3.30 for fish and 3.00–3.28 for mammals and birds can produce systematically skewed isotopic compositions and may lead to significant interpretative errors. These findings are followed by a review of protocols for improving C:N<sub>Atomic</sub> criteria for modern collagen extracts.</p> <p><strong>(4)</strong> Given the tremendous conservation and environmental policy-informing potential that retrospective isotopic analyses of collagen from contemporary and archived vertebrate tissues have for addressing pressing questions about long-term environmental conditions and species behaviours, it is critical that QC criteria tailored to modern tissues are established.</p>
Data from: Dissolved nitrogen uptake versus nitrogen fixation: Mode of nitrogen acquisition affects stable isotope signatures of a diazotrophic cyanobacterium and its grazer
<p>Field studies suggest that changes in the stable isotope ratios of phytoplankton communities can be used to track changes in the utilization of different nitrogen sources, i.e., to detect shifts from dissolved inorganic nitrogen (DIN) uptake to atmospheric nitrogen (N<sub>2</sub>) fixation by diazotrophic cyanobacteria as an indication of nitrogen limitation. We explored changes in the stable isotope signature of the diazotrophic cyanobacterium <em>Trichormus variabilis</em> in response to increasing nitrate (NO<sub>3</sub><sup>-</sup>) concentrations (0 to 170 mg L<sup>-1</sup>) under controlled laboratory conditions. In addition, we explored the influence of nitrogen utilization at the primary producer level on trophic fractionation by studying potential changes in isotope ratios in the freshwater model <em>Daphnia magna</em> feeding on the differently grown cyanobacteria. We show that <em>δ</em><sup>15</sup>N values of the cyanobacterium increase asymptotically with DIN availability, from -0.7 ‰ in the absence of DIN (suggesting N<sub>2</sub> fixation) to 2.9 ‰ at the highest DIN concentration (exclusive DIN uptake). In contrast, <em>δ</em><sup>13</sup>C values of the cyanobacterium did not show a clear relationship with DIN availability. The stable isotope ratios of the consumer reflected those of the differently grown cyanobacteria but also revealed significant trophic fractionation in response to nitrogen utilization at the primary producer level. Nitrogen isotope turnover rates of <em>Daphnia</em> were highest in the absence of DIN as a consequence of N<sub>2</sub> fixation and resulting depletion in <sup>15</sup>N at the primary producer level. Our results highlight the potential of stable isotopes to assess nitrogen limitation and to explore diazotrophy in aquatic food webs.</p>
Occurrence patterns of crop-foraging sika deer distribution in an agriculture-forest landscape revealed by nitrogen stable isotopes
<p>Conflicts arising from the consumption of anthropogenic foods by wildlife are increasing worldwide. Conventional tools for evaluating the spatial distribution pattern of large terrestrial mammals that consume anthropogenic foods have various limitations, despite their importance in management to mitigate conflicts. In this study, we examined the spatial distribution pattern of crop-foraging sika deer by performing nitrogen stable isotope analyses of bone collagen. We evaluated whether crop-foraging deer lived closer to agricultural crop fields during the winter and spring, when crop production decreases. We found that female deer in proximity to agricultural crop fields during the winter and spring were more likely to be crop-foraging individuals. Furthermore, the likelihood of crop consumption by females decreased by half as the distance to agricultural crop fields increased to 5-10 km. We did not detect a significant trend in the spatial distribution of crop-foraging male deer. The findings of spatial distribution patterns of crop-foraging female deer will be useful for the establishment of management areas, such as zonation, for efficient removal of them.</p>
Supplementary Data to: "Organic Phases in Bivalve (Arctica islandica) Shells: Their Bulk and Amino Acid Nitrogen Stable Isotope Compositions" in Geochemistry, Geophysics, Geosystems
<p>This dataset contains the data generated for publication "Organic Phases in Bivalve (<em>Arctica islandica</em>) Shells: Their Bulk and Amino Acid Nitrogen Stable Isotope Compositions", including the following files:</p> <p>Specimen_ID_shell_morphology_measurement: Description of locality of collection, shell ID, shell working ID and the shell morphology measurement which can be referred to Section 2.1 of the manuscript.</p> <p>EA_IRMS_raw_error_propagate: This file contains data showing the m/z 28 signal intensities of all measurements and the error propagation that mentioned in Section 2.3 of the manuscript. It also includes the calculation of analytical accuracy and precision. </p> <p>Statistics: The file contains the summary of statistical analyses performed in this manuscript. Refer to Section 2.7 and 3.3.</p> <p>Recovery: It contains the recovery of the total nitrogen contents or amino acid concentrations after different treatments and this was mentioned in Section 4.1 of the manucript.</p> <p>Mass_balance: The computation procedure that showed in Section 3.4 and discussed in Section 4.3 can be traced in this file.</p> <p>GC-C-IRMS_long_term_sd: The file shows the long-term precision of GC-C-IRMS computed from the mixed standard of amino acids </p> <p>BSIA_d15N: The file contains all the bulk d15N data that used in this manuscript.</p> <p>CSIA_d15N: The file contains all the amino acid d15N data that used in this manuscript.</p> <p>AA_Composition: The file shows AA percent data that used in this manuscript.</p> <p><br> For details see main text of the manuscript.</p> <p><br> </p>
Feather nitrogen and carbon stable isotope (d15N and d13C) values for Golden-crowned Sparrows
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