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8 results for “Northern Spotted Owls”
Supplemental dataset for Northern Spotted Owl (<i>Strix occidentalis caurina</i>) genome assembly version 1.0
<p><strong>StrOccCau_1.0_nuc.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the file that we deposited at DDBJ/ENA/GenBank as a Whole Genome Shotgun (WGS) project under accession NIFN00000000. It is is the file that you will most likely want to download if you would like to perform an alignment to this genome assembly. This file is the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without any contigs and scaffolds less than 1,000 nt and also without the contigs and scaffolds that we identified either as the mitochondrial genome sequence or as contaminant sequences.</p> <p><strong>StrOccCau_1.0_nuc_masked.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the repeat-masked (hard-masked) assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without any contigs and scaffolds less than 1,000 nt and also without the contigs and scaffolds that we identified either as the mitochondrial genome sequence or as contaminant sequences.</p> <p><strong>StrOccCau_1.0_mito.fa</strong> : This FASTA format file is the mitochondrial-genome-derived scaffold from the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012).</p> <p><strong>StrOccCau_1.0.gff.bz2</strong> : This gff format file compressed this file with bzip2 contains the gene annotations of StrOccCau_1.0_nuc.fa.</p> <p><strong>StrOccCau_1.0_transcripts.fa.bz2</strong> : This FASTA format file compressed this file with bzip2 contains the sequences of the gene transcript sequences of the genes annotated in StrOccCau_1.0.gff.</p> <p><strong>StrOccCau_1.0_proteins.fa.bz2</strong> : This FASTA format file compressed this file with bzip2 contains the protein sequences of the genes annotated in StrOccCau_1.0.gff.</p> <p><strong>StrOccCau_1.0_RM_homology_includes_LowComplexity.out.bz2</strong> : This file provides the repeat annotations produced by the homology-based masking of StrOccCau_1.0_nuc.fa that included masking of low complexity regions and simple repeats. We compressed this file with bzip2.</p> <p><strong>StrOccCau_1.0_RM_DeNovo_includes_LowComplexity.out</strong> : This file provides the repeat annotations produced by the de novo masking (which followed after first performing homology-based masking) of StrOccCau_1.0_nuc.fa that included masking of low complexity regions and simple repeats.</p> <p><strong>StrOccCau_1.0_RM_homology_no_LowComplexity.out.bz2</strong> : This file provides the repeat annotations produced by the homology-based masking of StrOccCau_1.0_nuc.fa that did not include masking of low complexity regions and simple repeats. We compressed this file with bzip2.</p> <p><strong>StrOccCau_1.0_RM_DeNovo_no_LowComplexity.out</strong> : This file provides the repeat annotations produced by the de novo masking (which followed after first performing homology-based masking) of StrOccCau_1.0_nuc.fa that did not include masking of low complexity regions and simple repeats.</p> <p><strong>StrOccCau_1.0_alignments_of_light_associated_genes.txt</strong> : This file provides alignments of light-associated gene orthologs as well as assemblies of transcriptome sequences in NEXUS format.</p> <p><strong>StrOccCau_1.0_nuc_masked_SpottedBarredOwl_variant_file.vcf.bz2</strong> : This is a raw, unfiltered variant call format file compressed with bzip2 that was generated after aligning both spotted owl and barred owl short read data aligned to StrOccCau_1.0_nuc_masked.fa.</p> <p><strong>StrOccCau_0.1.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012).</p> <p><strong>StrOccCau_0.1_masked.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the repeat-masked assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012).</p> <p><strong>StrOccCau_0.2.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without any contigs and scaffolds less than 1,000 nt.</p> <p><strong>StrOccCau_0.2_masked.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the repeat-masked assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without any contigs and scaffolds less than 1,000 nt.</p> <p><strong>StrOccCau_GapCloser_output_NoContamNoMito.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without the contigs and scaffolds that we later identified either as the mitochondrial genome sequence or as contaminant sequences.</p> <p><strong>Citations</strong> - if you utilize these data, please include these citations:</p> <p>Hanna ZR., Henderson JB., Wall JD., Emerling CA., Fuchs J., Runckel C., Mindell DP., Bowie RCK., DeRisi JL., Dumbacher JP. 2017a. Supplemental dataset for Northern Spotted Owl (<em>Strix occidentalis caurina</em>) genome assembly version 1.0. <em>Zenodo</em>. DOI: 10.5281/zenodo.822859.</p> <p>Hanna ZR., Henderson JB., Wall JD., Emerling CA., Fuchs J., Runckel C., Mindell DP., Bowie RCK., DeRisi JL., Dumbacher JP. 2017b. Northern Spotted Owl (Strix occidentalis caurina) Genome: Divergence with the Barred Owl (<em>Strix varia</em>) and Characterization of Light-Associated Genes. <em>Genome Biology and Evolution</em> 9:2522–2545. DOI: 10.1093/gbe/evx158.</p>
Adapter sequences used for trimming of genomic sequences in the assembly of the Northern Spotted Owl (<i>Strix occidentalis caurina</i>) genome assembly version 1.0
<p>These files provide the sequences of the adapters used in the construction of the genomic libraries Hanna et al. (2017a) sequenced and used to assemble the Northern Spotted Owl (<em>Strix occidentalis caurina</em>) genome assembly version 1.0 (Hanna et al. 2017b). These files also contain relevant supplemental adapter sequences from the adapter files included with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011595_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011595. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" and "NexteraPE-PE.fa" files distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011596_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011596. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" and "NexteraPE-PE.fa" files distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011597_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011597. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" and "NexteraPE-PE.fa" files distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011614_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011614. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" and "NexteraPE-PE.fa" files distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011615_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011615. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" file distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011616_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011616. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" file distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).<br> <br> <strong>SRR4011617_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011617. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" file distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p>
Feather corticosterone reveals developmental challenges in a long‐term study of juvenile northern spotted owls
<p>1. Corticosterone is a steroid hormone integral to a variety of physiological pathways and is strongly associated with the vertebrate stress–response. In avian species, circulating corticosterone is sequestered into developing feathers and is used as an indicator of energy allocation during feather growth and widely applied in conservation physiology.</p> <p>2. The northern spotted owl (<i>Strix occidentalis caurina</i>) is a federally threatened old–growth forest obligate of conservation concern endemic to the Pacific Northwest of the United States and Canada. The effects of landscape characteristics and individual variation on early development in spotted owls remain unstudied despite long recognition of this knowledge gap and its potential importance to species conservation.</p> <p>3. We quantified corticosterone concentrations in 4,720 feathers from 1,056 juvenile spotted owls across seven study areas between 2001 and 2017. We used an information–theoretic approach to examine the environmental and individual factors related to feather corticosterone in juvenile spotted owls as an indicator of challenges during early development.</p> <p>4. Feather corticosterone was positively related to temperature and precipitation, and negatively related to juvenile mass at banding. We found strong support for an interaction between mass and precipitation, with greater amounts of precipitation being associated with higher levels of feather corticosterone in lighter juveniles. The temperature and precipitation metric with the strongest relationship with feather corticosterone occurred during the fledging period, suggesting that this period presents an energetic challenge for juvenile spotted owls. Greater juvenile mass decreased the effect of precipitation, suggesting that greater mass was important for juveniles to maintain homeostasis during fledgling.</p> <p>5. Feather corticosterone in juvenile spotted owls provided insights to the challenges faced during early development, adding to our understanding of spotted owl life history and potential for population recovery.</p>
Feather corticosterone reveals developmental challenges in a long‐term study of juvenile northern spotted owls
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Conspecific and congeneric interactions shape increasing rates of breeding dispersal of northern spotted owls
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Social status, forest disturbance, and Barred Owls shape long-term trends in breeding dispersal distance of Northern Spotted Owls
<p>Dispersal among breeding sites in territorial animals (i.e. breeding dispersal) is driven by numerous selection pressures, including competition and spatiotemporal variation in habitat quality. The scale and trend of dispersal movements over time may signal changing conditions within the population or on the landscape. We examined 2,158 breeding dispersal events from 694 male and 608 female individually-marked Northern Spotted Owls (<i>Strix occidentalis caurina</i>) monitored over 28 years on seven study areas to assess the relative importance of individual (sex, experience), reproductive (annual productivity, mate availability), and environmental (forest alteration, presence of competitor) sources of variation in breeding dispersal distance. Median breeding dispersal distance was 3.17 km, with 99% of all breeding dispersal events less than 37 km. Mean annual dispersal distances increased by 2.43 km in Oregon and 9.40 km in Washington between 1990 and 2017, which coincided with increases in annual detections of non-native Barred Owl (<i>S. varia</i>). Frequency of breeding dispersal events, both among and within individuals, also increased over time. Female owls moved farther than males (median of 3.26 km and 3.10 km respectively), and birds with less experience (territory tenure) moved farther than those with more experience. Owls that were single in the year prior to dispersal moved 13–31% farther than those paired prior to dispersal. The greatest environmental change occurring over the course of our study was the expansion of Barred Owl populations. Breeding dispersal distance was positively related to Barred Owls in the study area and disturbance within the originating territory. While it appears that social factors continue to be important drivers of breeding dispersal distance in Spotted Owls, increased competition from Barred Owls and habitat alteration have a contributing effect. Increased breeding dispersal distances should be of concern for conservation efforts and considered in population monitoring because changing dispersal behavior may lead to higher rates of mortality and/or emigration from historic study areas.</p>
Social status, forest disturbance, and Barred Owls shape long-term trends in breeding dispersal distance of Northern Spotted Owls
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Data from: Passive acoustic monitoring effectively detects Northern Spotted Owls and Barred Owls over a range of forest conditions
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