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12 results for “Nosema ceranae”
Fig. 3. 2 in Spores of Paenibacillus larvae, Ascosphaera apis, Nosema ceranae and Nosema apis in bee products supervised by the Brazilian Federal Inspection Service
Fig. 3. 2% agarose gel stained with SYBR Safe of the multiplex PCR products from royal jelly samples (1–10) obtained from markets of the state of São Paulo, Brazil. M, ® molecular marker 100 pb (Invitrogen); C+, positive control for N. ceranae (218 pb), N. apis (321 pb), A. apis (485 pb) and P. larvae (700 pb); C−, negative control.
Fig. 4. 2 in Spores of Paenibacillus larvae, Ascosphaera apis, Nosema ceranae and Nosema apis in bee products supervised by the Brazilian Federal Inspection Service
Fig. 4. 2% agarose gel stained with SYBR Safe of the multiplex PCR products from honey samples (1–17) obtained from markets of the state of São Paulo, Brazil. M, ® molecular marker 100 pb (Invitrogen); C+, positive control for N. ceranae (218 pb), N. apis (321 pb), A. apis (485 pb) and P. larvae (700 pb); C−, negative control.
Fig. 5. 2 in Spores of Paenibacillus larvae, Ascosphaera apis, Nosema ceranae and Nosema apis in bee products supervised by the Brazilian Federal Inspection Service
Fig. 5. 2% agarose gel stained with SYBR Safe of the multiplex PCR products from pollen samples (1–10) obtained from markets of the state of São Paulo, Brazil. M, ® molecular marker 100 pb (Invitrogen); C+, positive control for N. ceranae (218 pb), N. apis (321 pb), A. apis (485 pb) and P. larvae (700 pb); C−, negative control.
Fig. 1 in Bombus brasiliensis Lepeletier (Hymenoptera, Apidae) infected with Nosema ceranae (Microsporidia)
Fig. 1. (A) The 32 surveyed localities in northeastern Argentina. Formosa province [Fo]: (1) Ingeniero Juárez; (2) Laguna Yema; (3) Las Lomitas; (4) Bañado La Estrella; (5) Posta Cambio Zalazar; (6) Pozo del Tigre; (7) Colonia Perin; (8) Ibarreta; (9) Palo Santo; (10) Pirané; (11) Gran Guardia; (12) El Colorado. Chaco province [Ch]: (13) J.J. Castelli; (14) Presidencia Roque Saenz Peña; (15) Colonia Elisa; (16) 38 km North of Resistencia; (17) Resistencia. Corrientes province [Co]: (18) Corrientes; (19) Estero Santa Lucía; (20) Colonia Carlos Pellegrini; (21) Laguna Iberá; (22) Santo Tomé. Misiones province [Mi]: (23) Posadas; (24) Leandro N. Alem; (25) Cuña Pirú; (26) Aristóbulo del Valle; (27) El alcázar; (28) Montecarlo; (29) 30 km East of María Magdalena; (30) Wanda; (31) Urugua-í. (Ɨ) indicates Puerto Iguazú, the only locality where six Bombus brasiliensis workers were found. (B) Habitat where B. brasiliensis was found. (C) Two distended midgut cells of B. brasiliensis with spores of Nosema ceranae inside.
Fig. 2 in PREVALENCE OF NOSEMA CERANAE (MICROSPORIDIA) IN THE APIS MELLIFERA MELLIFERA BEE COLONIES FROM LONG TIME ISOLATED APIARIES OF SIBERIA
Fig. 2. Distribution of Nosema species in apiaries throughout the Krasnoyarsk Krai (dots
Effects of Nosema ceranae (DISSOCIODIHAPLOPHASIDA: Nosematidae) and flupyradifurone on olfactory learning in honey bees, Apis mellifera (HYMENOPTERA: Apidae)
<p>The health of insect pollinators, particularly the honey bee, <em>Apis mellifera </em>(Linnaeus, 1758), is a major concern for agriculture and ecosystem health. In response to mounting evidence supporting the detrimental effects of neonicotinoid pesticides on pollinators, a novel “bee safe” butenolide compound, flupyradifurone (FPF) has been registered for agricultural use. Although FPF is not a neonicotinoid, like neonicotinoids, it is an excitotoxic nicotinic acetylcholine receptor agonist. In addition, <em>A. mellifera</em> faces threats from pathogens, such as the microsporidian endoparasite,<em> Nosema ceranae</em> (Fries et al., 1996). We therefore sought (1) to increase our understanding of the potential effects of FPF on honey bees by focusing on a crucial behavior, the ability to learn and remember an odor associated with a food reward, and (2) to test for a potential synergistic effect on such learning by exposure to FPF and infection with <em>N. ceranae. </em>We found little evidence that FPF significantly alters learning and memory at short-term field-realistic doses. However, at high doses and at chronic, field-realistic exposure, FPF did reduce learning and memory in an olfactory conditioning task. Infection with <em>N. ceranae</em> also reduced learning, but there was no synergy (no significant interaction) between<em> N. ceranae</em> and exposure to FPF. These results suggest the importance of continued studies on the chronic effects of FPF.</p>
Data from: Nosema ceranae can infect honey bee larvae and reduces subsequent adult longevity
Nosema ceranae causes a widespread disease that reduces honey bee health but is only thought to infect adult honey bees, not larvae, a critical life stage. We reared honey bee (Apis mellifera) larvae in vitro and provide the first demonstration that N. ceranae can infect larvae and decrease subsequent adult longevity. We exposed three-day-old larvae to a single dose of 40,000 (40K), 10,000 (10K), zero (control), or 40K autoclaved (control) N. ceranae spores in larval food. Spores developed intracellularly in midgut cells at the pre-pupal stage (8 days after egg hatching) of 41% of bees exposed as larvae. We counted the number of N. ceranae spores in dissected bee midguts of pre-pupae and, in a separate group, upon adult death. Pre-pupae exposed to the 10K or 40K spore treatments as larvae had significantly elevated spore counts as compared to controls. Adults exposed as larvae had significantly elevated spore counts as compared to controls. Larval spore exposure decreased longevity: a 40K treatment decreased the age by which 75% of adult bees died by 28%. Unexpectedly, the low dose (10K) led to significantly greater infection (1.3 fold more spores and 1.5 fold more infected bees) than the high dose (40K) upon adult death. Differential immune activation may be involved if the higher dose triggered a stronger larval immune response that resulted in fewer adult spores but imposed a cost, reducing lifespan. The impact of N. ceranae on honey bee larval development and the larvae of naturally infected colonies therefore deserve further study.
Data from: Nosema ceranae can infect honey bee larvae and reduces subsequent adult longevity
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Brain transcriptome of honeybees (Apis mellifera) infected by Nosema ceranae and/or Black Queen Cell Virus
GEO Series GSE81664. Apis mellifera. 11 samples. Type: Expression profiling by high throughput sequencing.
Brain transcriptomics of honey bee parasitized by Varroa destructor or Nosema ceranae
GEO Series GSE41109. Apis mellifera. 6 samples. Type: Expression profiling by high throughput sequencing.
Fig. 1 in PREVALENCE OF NOSEMA CERANAE (MICROSPORIDIA) IN THE APIS MELLIFERA MELLIFERA BEE COLONIES FROM LONG TIME ISOLATED APIARIES OF SIBERIA
Fig. 1. The map of localization of the Yenisei population and studied apiaries (dots 1–6)
Trancriptome analysis of honey bees (Apis mellifera) infected with Nosema ceranae
GEO Series GSE25455. Apis mellifera. 12 samples. Type: Expression profiling by genome tiling array.
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