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16 results for “Octopus vulgaris”
Figure 5 in Morphofunctional characterization and antibacterial activity of haemocytes from Octopus vulgaris
Figure 5. Micrograph of Octopus vulgaris cultured haemocytes showing phagocytic activity. Arrows indicate the phagocytosed yeast particles. Scale bar = 10 μm.
Figure 8 in Morphofunctional characterization and antibacterial activity of haemocytes from Octopus vulgaris
Figure 8. Bactericidal activity of Octopus vulgaris haemocyte crude methanolic acid extract (HMAE). The graph shows the inibition of E. coli growth in the presence of increasing concentrations of HMAE: at 0.2 μg/ml HMAE the bacterial growth is dramatically reduced, while at 0.8 μg/ml it is completely inhibited.
Figure 1 in Morphofunctional characterization and antibacterial activity of haemocytes from Octopus vulgaris
Figure 1. Differential interference contrast micrographs showing different types of haemocytes in culture plate: (A) haemoblast-like cell without pseudopodia; (B) two hyalinocytes connecting each other with pseudopodia; (C) well-attached granulocyte showing dendritic pseudopodia formation; (D) graph showing the ratio among the three haemocyte types. Scale bar = 5 μm.
Figure 4 in Morphofunctional characterization and antibacterial activity of haemocytes from Octopus vulgaris
Figure 4. Light microscopy micrographs of Octopus vulgaris haemocytes processed with enzymatic histochemistry analysis: (A) granulocytes showing a peroxidase activity localized as dark brown deposits; (B) haemoblast-like cells with phenol oxidase activity; (C) hyalinocytes with phenol oxidase activity; (D) granulocytes showing phenol oxidase activity. Scale bar = 5 μm.
Figure 3 in Morphofunctional characterization and antibacterial activity of haemocytes from Octopus vulgaris
Figure 3. Light microscopy micrographs of haemocytes stained with Giemsa/May–Grünwald stain: (A) acidophilic haemoblast-like cells (arrowheads) and granulocytes with acidophilic cytoplasm and cells (arrows); (B) hyalinocytes with basophilic nucleus and cytoplasm filled with vacuoles and few granules; (C) granulocytes cells with basophilic cytoplasm. Scale bar 5 μm.
Genetic monitoring on the world's first MSC eco-labeled common octopus (O. vulgaris) fishery in western Asturias, Spain
<p><strong>Allele frequencies file containing:</strong></p> <ul> <li>15 populations</li> <li>13 microsatellite markers</li> </ul> <p><em><strong>Populations:</strong></em></p> <ul> <li>21PS: Pasaia (Basque country, Spain). Fishery season (FS): 2020-21</li> <li>18RB: Ribadesella (Asturias, Spain). FS: 2017-18</li> <li>21RB: Ribadesella (Asturias, Spain). FS: 2020-21</li> <li>18CU: Cudillero (Asturias, Spain). FS: 2017-18</li> <li>21CU: Cudillero (Asturias, Spain). FS: 2020-21</li> <li>07PV: Puerto de Vega (Asturias, Spain). FS: 2006-07</li> <li>18PV: Puerto de Vega (Asturias, Spain). FS: 2017-18</li> <li>21PV: Puerto de Vega (Asturias, Spain). FS: 2020-21</li> <li>18TP: Tapia de Casariego (Asturias, Spain). FS: 2017-18</li> <li>21TP: Tapia de Casariego (Asturias, Spain). FS: 2020-21</li> <li>21BU: Bueu (Galicia, Spain). FS: 2020-21</li> <li>07OL: Olhão (Algarve, Portugal). FS: 2006-07</li> <li>21OL: Olhão (Algarve, Portugal). FS: 2020-21</li> <li>21SA: San Andrés (Canary Islands, Spain). FS: 2020-21</li> <li>21BC: Barcelona (Catalonia, Spain). FS: 2020-21</li> </ul> <p><em><strong>Microsatellite markers (GenBank accession number):</strong></em></p> <p>OCT08 (AF197132); VULG15 (LC003035); VULG14 (LC003034); VULG07 (LC003028); OVUL10 (JN579699); VULG12 (LC003032); VULG13 (LC003033); VULG06 (LC003027); OVUL09 (JN579698); VULG04 (LC003026); OVUL08 (JN579697); OV10 (AF197134); VULG10 (LC003030).</p>
Octopus vulgaris, Sepia officinalis, Loligo vulgaris and Illex coindetii early life phases Light Sheet Fluerescence Microscopy (LSFM) 3D scans.
<p>Acronyms: OV: <em>Octopus vulgaris</em>, SO: <em>Sepia officinalis</em>, LV: <em>Loligo vulgaris</em>, IC: <em>Illex coindetii</em>, DPH: Days Post-Hatching.</p> <p>Two detection objectives were used, depending on sample size, a 4x/0.28 NA Olympus XLFLUOR4x/340 objective (0, 5, 10, 19 DPH <em>Octopus vulgaris</em> individuals,<em> Loligo vulgaris</em> and<em> Illex coindetii</em>) and a Nikon 10x/0.5 NA CFI Plan Apochromat 10xC Glyc (Rest of the samples). For illumination, two 4x/0.95 NA Nikon CFI Plan Apo Lambda 4x were used when using the 10x detection objective and two 4x/0.13 NA Nikon Plan Fluor illumination objectives were used when using the 4x detection objective. </p> <p>Microscope: MuVi SPIM (Luxendo), LCS SPIM (Luxendo, only <em>Sepia officinalis</em> and 60 DPH <em>Octopus vulgaris</em> individuals).</p> <p>All the data has been scaled in order to reduce file sizes. Full size stacks can be requested to dgvilar@gmail.com.</p>
FIGURE 1 in Octopus djinda (Cephalopoda: Octopodidae): a new member of the Octopus vulgaris group from southwest Australia
FIGURE 1. Geographic distribution of Octopus djinda, sp. nov. along the southwest Australian coast, from Shark Bay (north) to Cape Le Grand (southeast). Sampling localities are shown with white triangles: Ge, Geraldton; Ma, Mandurah; Es, Esperance. The distribution of O. tetricus is also shown along the east coast of Australia and northern New Zealand. Scale, 1,000 km.
FIGURE 5 in Octopus djinda (Cephalopoda: Octopodidae): a new member of the Octopus vulgaris group from southwest Australia
FIGURE 5. Octopus djinda, sp. nov. upper (A) and lower (B) beak. SEM image of the radula is shown under 25 times magnification (C). Scale, 10 mm (A, B), 1 mm (C).
FIGURE 4 in Octopus djinda (Cephalopoda: Octopodidae): a new member of the Octopus vulgaris group from southwest Australia
FIGURE 4. Digestive tract of a male Octopus djinda, sp. nov. specimen: Ad, dorsal aorta; BM, buccal mass; Cae, caecum; Cr, crop; DG, digestive gland; I, intestine; Oe, oesophagus; SGa, anterior salivary gland; SGp, posterior salivary gland; S, stomach; R, rectum. Scale, 20 mm.
FIGURE 3 in Octopus djinda (Cephalopoda: Octopodidae): a new member of the Octopus vulgaris group from southwest Australia
FIGURE 3. Male Octopus djinda, sp. nov. holotype specimen (WAM S.89010) from Esperance Bay, Australia. Scale, 30 mm.
FIGURE 6 in Octopus djinda (Cephalopoda: Octopodidae): a new member of the Octopus vulgaris group from southwest Australia
FIGURE 6. Octopus djinda, sp. nov. male reproductive tract shown in original orientation (A), and after rearrangement (B, C): AG, accessory gland; D, diverticulum; MG, mucilaginous gland; SG, spermatophore gland; SS, spermatophore sac; T, testis; TO, terminal organ. Female reproductive tract is also shown (D): Ov, ovary; Od, distal oviduct; OG, oviductal gland; Op, proximal oviduct. Scale, 10 mm.
Figure 7 in Morphofunctional characterization and antibacterial activity of haemocytes from Octopus vulgaris
Figure 7. Bactericidal activity (percentage of killing) of Octopus vulgaris haemocytes in the presence of different bacteria.
Figure 2 in Morphofunctional characterization and antibacterial activity of haemocytes from Octopus vulgaris
Figure 2. Light microscopy micrographs of haemocytes stained with acidic toluidine blue revealing their metachromatic property: (A) in vivo haemoblast-like cell; (B) in vivo hyalinocytes containing blue granules; (C) in vivo granulocyte containing red and blue granules; (D) in vivo haemocytes aggregate; (E) fixed haemocytes connected by their filopodia [hyalinocytes (arrowheads) and granulocytes (arrows)]. Scale bar = 5 μm.
Figure 6 in Morphofunctional characterization and antibacterial activity of haemocytes from Octopus vulgaris
Figure 6. Flow cytometric light scatter plots, DNA content and cell cycle analysis of haemocytes from Octopus vulgaris. (A) The left plot shows flow cytometric forward-angle light scatter (FSC) versus side-angle light scatter (SSC) plot on "ungated" sample. The percentage of each population is indicated. The centre plot shows pulse width (PI-W) versus area (PI-A) plot of the PI (propidium iodide) channel used to distinguish between single cells and aggregates. Single cells (G0/1 or G2/M) will have similar pulse width (transit time) values. Aggregates will have larger width values and can be easily seen on the plot to the right of the single cell region. Single cells have been gated (left plot). The right scatter plot shows the FSC versus SSC plot of PIgated cells (single cells). Populations and percentage are indicated; (B) FL2–Area histograms and determination of the cell cycle of each population. The Dean–Jett–Fox model was used to define the G1, S, and G2 phases of the cell cycle. RMS refers to Root Mean Square.
FIGURE 2 in Octopus djinda (Cephalopoda: Octopodidae): a new member of the Octopus vulgaris group from southwest Australia
FIGURE 2. Live Octopus djinda, sp. nov. specimen. Photo, Mark Norman.
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