Find research datasets worth reusing

opencc-by-4.0Dec 2015View details →

Fig. 3 in Short Communication High-Density Cultivation of the Marine Ciliate Uronema marinum (Ciliophora, Oligohymenophorea) in Axenic Medium

Fig. 3. Growth chart of U. marinum in PGY medium and bacterized filtered seawater. The density of ciliate cells was measured every 12 hours after inoculation using a hemocytometer. The final data points are 419 cells/μl in PGY medium and 11 cells/μl in bacterized filtered seawater.

opencc-by-4.0Dec 2015View details →

Fig. 2 in Short Communication High-Density Cultivation of the Marine Ciliate Uronema marinum (Ciliophora, Oligohymenophorea) in Axenic Medium

Fig. 2. PCR amplification of the bacterial SSU-rDNA on 1% agarose gel. A1, A2, and A3 are parallel samples extracted from the axenic culture in PGY medium. B1, B2, and B3 are parallel samples extracted from the culture in bacterized filtered seawater. M – DNA ladder.

opencc-by-4.0Dec 2015View details →

Fig. 1 in Short Communication High-Density Cultivation of the Marine Ciliate Uronema marinum (Ciliophora, Oligohymenophorea) in Axenic Medium

Fig. 1. Uronema marinum from life (A, B, E, F), after protargol (C, D) and DAPI-staining (G, H). A, B – lateral-ventral view of typical cell (B, from Pan et al. 2010); C, D – ventral and dorsal view of the same specimen (from Pan et al. 2010); E – 72 hours after inoculating into PGY medium; F – 168 hours after inoculating into PGY medium; G – Uronema marinum in axenic culture, demonstrating the absence of bacteria; H – Uronema marinum in bacterized filtered seawater cultivating system, arrowheads indicate bacteria that are active in the realtime viewing conditions; M1–3 – membranelles 1–3, PM – paroral membrane, Sc – scutica. Scale bars: 20 μm.

Fig. 5 in Redescription of Dexiotricha colpidiopsis (Kahl, 1926) Jankowski, 1964 (Ciliophora, Oligohymenophorea) from a Hot Spring in Iceland with Identification Key for Dexiotricha species

Fig. 5. Dexiotricha species from live (A, D, F, G) and silver-stained specimens (B, C, E, H–L). (A–C) D. elliptica (from Fan et al. 2014). (D, E) D. tranquilla (from Augustin and Foissner 1992). (F) D. polystyla (from Foissner 1987). (G, H) D. granulosa (G from Behrend 1916; H from Fan et al. 2014). (I) D. media (from Peck 1974). (J, K) D. colpidiopsis (J from Fauré-Fremiet 1968; H from Jankowski 1964). (L) D. raikovi (from Jankowski 1964). Scale bars: 20 µm.

Fig. 2 in Redescription of Dexiotricha colpidiopsis (Kahl, 1926) Jankowski, 1964 (Ciliophora, Oligohymenophorea) from a Hot Spring in Iceland with Identification Key for Dexiotricha species

Fig. 2. Dexiotricha colpidiopsis from live (A–C) and after protargol-staining (D–F). (A) Ventrolateral view of a typical specimen showing the subterminal contractile vacuole (arrow). (B) Ventral view (from Kahl 1926). (C) Right lateral view showing the transverse row of cilia (arrows). (D) Ciliature of oral region. (E, F) Ventral and dorsal views of type specimen. CC, caudal cilium; M1–3, membranelles 1–3; Ma, macronucleus; Mi, micronucleus; PK, postoral kineties; PM, paroral membrane; Sc, scutica; SK, somatic kineties; SK1, first somatic kinety on right margin of buccal cavity; SKn, first somatic kinety on left margin of buccal cavity. Scale bars: 25 µm.

Fig. 4 in Redescription of Dexiotricha colpidiopsis (Kahl, 1926) Jankowski, 1964 (Ciliophora, Oligohymenophorea) from a Hot Spring in Iceland with Identification Key for Dexiotricha species

Fig. 4. Phylogenetic tree inferred by Maximum-likelihood (ML) analyses of the small SSU rRNA gene sequences. The new sequence is highlighted in bold. Numbers at the nodes are the bootstrap values of the ML and BI analyses, respectively. The mark "-" indicates discrepancies in the topologies of the ML and BI trees; thus, only the values of ML are shown in these cases. The scale bar corresponds to 5 substitutions per 100 nucleotide positions.

Fig. 3 in Redescription of Dexiotricha colpidiopsis (Kahl, 1926) Jankowski, 1964 (Ciliophora, Oligohymenophorea) from a Hot Spring in Iceland with Identification Key for Dexiotricha species

Fig. 3. Photomicrographs of Dexiotricha colpidiopsis from live (A–D; A with bright field illumination, C–D with differential interference contrast microscopy), after dry silver nitrate staining (E), and after protargol-impregnation (F–I). (A, B) Ventrolateral views showing the subterminal contractile vacuole (arrows) and the caudal cilium (arrowhead). (C) Right lateral view showing the transverse row of cilia (arrows). (D) Slightly compressed specimens showing the subterminal contractile vacuole (arrows). (E) Showing the position of the contractile vacuole pore (arrow). (F, G) Ventral and dorsal views of the type specimen. (H, I) Right and left lateral views. M1–3, membranelles 1–3; Ma, macronucleus; Mi, micronucleus; PK, postoral kinety, PM, paroral membrane. Scale bars: 25 µm.

Fig. 5 in Morphological Redescription and SSU rDNA-based Phylogeny of Two Freshwater Ciliates, Uronema nigricans and Lembadion lucens (Ciliophora, Oligohymenophorea), with Discussion on the Taxonomic Status of Uronemita sinensis

Fig. 5. Maximum likelihood (ML) tree inferred from SSU rDNA sequences, showing the position of Uronema nigricans and Lembadion lucens (red arrows). Numbers near branches denote ML bootstrap value/BI posterior probability. Asterisks (*) indicate topologies that differ between the ML and BI analyses. Fully supported (100%/1.00) branches are marked with solid circles. Question marks (?) in red color indicate that the two sequences are possibly misidentified. The scale bar corresponds to 5 substitutions per 100 nucleotide positions. All branches are drawn to scale. Systematic classification mainly follows Lynn (2008).

Fig. 4 in Morphological Redescription and SSU rDNA-based Phylogeny of Two Freshwater Ciliates, Uronema nigricans and Lembadion lucens (Ciliophora, Oligohymenophorea), with Discussion on the Taxonomic Status of Uronemita sinensis

Fig. 4. Photomicrographs of Lembadion lucens from life (A–D, with A in bright field illumination and others in DIC microscopy) and after protargol staining (E–K). (A, B) Ventral views of representative individuals, cell in B was slightly depressed. Arrow shows caudal cilia. (C) Dorsal view, arrowhead points to the contractile vacuole. (D) Apical view, revealing the shape of cross section. Arrow shows oral cilia. (E) Ventral view of a stained cell, showing the silverline system. (F, G) Ventral (F) and dorsal (G) view of a representative individual, to show the ciliature and nuclear apparatus. (H) Micronucleus (arrow) and macronucleus. (I) Ventral view of posterior portion. Arrows depict two basal body rows of caudal cilia. (J) Mid portion of dorsal view, illustrating the monokinetids and dikinetids. (K) Detailed view, arrows show the two separated pairs of basal bodies and arrowheads indicate the posterior ends of four gradually shortened outer rows of adoral membranelle. Scale bars: 30 μm.

Fig. 6 in Morphological Redescription and SSU rDNA-based Phylogeny of Two Freshwater Ciliates, Uronema nigricans and Lembadion lucens (Ciliophora, Oligohymenophorea), with Discussion on the Taxonomic Status of Uronemita sinensis

Fig. 6. Sequence comparison of the small subunit ribosomal RNA gene determined by BioEdit 7.0.5.2 (Hall 1999), showing the unmatched nucleotides of Uronema nigricans MF072399 and Lembadion lucens MF072398 with their sister sequences in the same clades, respectively (see Fig. 5). Nucleotide positions are given at the top of each column. Insertions and deletions are compensated by introducing alignment gaps (–). Numbers of unmatched sites (n) and sequence similarity percentages (%) compared with Uronema nigricans MF072399 and Lembadion lucens MF072398 are also supplied. The two sequences with question marks (?) are possibly misidentified. ID, identical; n, numbers of unmatched sites; %, sequence similarity percentages.

Fig. 3 in Morphological Redescription and SSU rDNA-based Phylogeny of Two Freshwater Ciliates, Uronema nigricans and Lembadion lucens (Ciliophora, Oligohymenophorea), with Discussion on the Taxonomic Status of Uronemita sinensis

Fig. 3. Photomicrographs of Uronema nigricans from life (A–E, in bright field illumination) and after protargol (F–M) staining. (A–C) Right ventrolateral views of representative individuals, with cell in C slightly depressed. Arrow in A shows contractile vacuole, while in C indicates macronucleus. (D) View of caudal portion of another cell, arrow points to caudal cilium. (E) Different body sizes, showing conspicuous apical plate. (F, G) Ventral views of stained individuals, indicating the structure of scutica. (H, I) Portion views of oral apparatus, revealing the number of kinety rows in M2 (two rows in H and three rows in I). (J, K) Ventral (J) and dorsal (K) view of a representative individual to show the ciliature and nuclear apparatus. Arrow in J points to basal body of caudal cilium, arrow in K indicating the apical plate. (L, M) Left ventrolateral (L) and right dorsolateral (M) view of another stained individual to show the ciliature and oral apparatus. Scale bars: 15 μm (A, B); 30 μm (E).

Fig. 1 in Morphological Redescription and SSU rDNA-based Phylogeny of Two Freshwater Ciliates, Uronema nigricans and Lembadion lucens (Ciliophora, Oligohymenophorea), with Discussion on the Taxonomic Status of Uronemita sinensis

Fig. 1. Map and sampling site. The yellow dots on the map and two corresponding photographs (A, B) show the collecting sites. (A) Fresh- water river in Shenzhen, southern China (22°32'19"N; 114°06'45"E). (B) Huguangyan Lake, an oligotrophic lake in Zhanjiang, southern China (21°08'38"N; 110°16'20"E).

zenodo36/100

Fig. 1 in Redescription of Dexiotricha colpidiopsis (Kahl, 1926) Jankowski, 1964 (Ciliophora, Oligohymenophorea) from a Hot Spring in Iceland with Identification Key for Dexiotricha species

Fig. 1. Sampling site in hot spring in Iceland (A, B) and sapropel sample (C).

FIGURE 10 in An annotated checklist of species in the family Lagenophryidae (Ciliophora, Oligohymenophorea, Peritrichia), With a brief review of their taxonomy, morphology, and biogeography

FIGURE 10. Geographical distributions of species of Setonophrys (numbers within squares) and Operculigera (numbers within circles).

FIGURE 7 in An annotated checklist of species in the family Lagenophryidae (Ciliophora, Oligohymenophorea, Peritrichia), With a brief review of their taxonomy, morphology, and biogeography

FIGURE 7. Characteristics of Operculigera. A, Dorsal view of O. montanea; B, Dorsal view of O. asymmetrica; C, Sagittal section of O. asymmetrica; D, Ventral view of operculum of O. insolita; E, Frontal view of operculum of O. seticola; F. Dorsal view of the anterior part of the lorica of O. madagascarensis showing the thickened ventral part of the operculum (see D, F) in outline (thin arrow). A–E, from Clamp 1991; F, from Clamp 1992. Abbreviations: AV, anterior half of vallum; AR, rim of aperture; Lo, dorsal wall of lorica; LP, peristomial lip; Op, operculum; PV, posterior half of vallum; thick arrow, knob-like thickening on ventral surface of operculum that is gripped by the edge of the peristomial lip. All scale bars = 10µm.

FIGURE 4 in An annotated checklist of species in the family Lagenophryidae (Ciliophora, Oligohymenophorea, Peritrichia), With a brief review of their taxonomy, morphology, and biogeography

FIGURE 4. Diversity of Lagenophrys spp. Intraspecific variation and adaptation for attachment to setae. A, B. Two morphs in a population of L. machaerigera that represent the extreme range of variation in outgrowth of the anterior lip of the lorica aperture and thickening of the posterior lip. C. Dorsal view of L. lenticula, which attaches to setae of the amphipod Hyalella with a posterior prolongation of the lorica (Ps, pseudostalk). D. Dorsal view of L. vaginicola, which attaches to caudal setae of harpacticoid copepods. A, from Clamp 1992; B–D, from Clamp 1991. All scale bars = 10µm.

FIGURE 6 in An annotated checklist of species in the family Lagenophryidae (Ciliophora, Oligohymenophorea, Peritrichia), With a brief review of their taxonomy, morphology, and biogeography

FIGURE 6. Characteristics of Clistolagenophrys, from Clamp 1991. A, Lateral view of individual with lorica aperture in open position; B, Lateral view of lorica with lorica aperture retracted and closed. Scale bars= 10µm.

FIGURE 2 in An annotated checklist of species in the family Lagenophryidae (Ciliophora, Oligohymenophorea, Peritrichia), With a brief review of their taxonomy, morphology, and biogeography

FIGURE 2. Characteristics of Lagenophrys. A. L. aselli on surface of pleopod of freshwater isopod. B. Lagenophrys ampulla, a typical representative of the genus, dorsal view. C. Lagenophrys labiata, lateral view. A, from Clamp 1988b; B–C, from Clamp 1991. Abbreviations: Ap, ampulla of cytostome (the cytopharynx leading into the cytoplasm is distended with a food vacuole); AL, anterior lip of lorica aperture; C, peristomial cilia (shown in outline); CT, crescentic thickening in wall of lorica anterior to lorica aperture; Cy, cytoplasm; EC, external collar of lorica aperture; ED, epistomial disk; If, infundibulum; Lo, dorsal wall of lorica; LR, rim of lorica; Ls, loricastome; LX, longitudinal axis of organism; MaN, macronucleus; MiN, micronucleus; PL, posterior lip of lorica aperture; PM, myoneme in edge of peristomial lip; TX, transverse axis of organism.

FIGURE 9 in An annotated checklist of species in the family Lagenophryidae (Ciliophora, Oligohymenophorea, Peritrichia), With a brief review of their taxonomy, morphology, and biogeography

FIGURE 9. Geographical distributions of known species of Lagenophrys (blue), Paralagenophrys (red), and Clistolagenophrys (arrowhead with 'C').

FIGURE 1 in An annotated checklist of species in the family Lagenophryidae (Ciliophora, Oligohymenophorea, Peritrichia), With a brief review of their taxonomy, morphology, and biogeography

FIGURE 1. Gross anatomy of peritrichs. Vorticella sp., a typical peritrich ciliate presented for comparison with lagenophryids. Redrawn from an unpublished drawing by Eugene B. Small. Abbreviations: C, peristomial cilia (shown in outline); Hk, haplokinety; LP, peristomial lip (dark band in interior is myoneme that constricts the lip over the peristome upon contraction); Pk, peristomial polykinety; P1, infundibular polykinety 1; P2, infundibular polykinety 2; P3, infundibular polikinety 3; SM, somatic myonemes; Sc, scopula; SS, stalk spasmoneme; TB, trochal band. Scale bar, 10µm.