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Figure 5 in Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

Figure 5: Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using ITS region. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.

Figure 5 in Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Figure 5: Bayesian consensus tree inferred from 28S D2/D3 under GTR+I+G model (−ln L = 4799.2056; AIC = 9618.4111; freqA = 0.2557; freqC = 0.182; freqG = 0.2892; freqT = 0.2732; R(a) = 0.6259; R(b) = 2.263; R(c) = 1.6102; R(d) = 0.5689; R(e) = 5.2205; R(f) = 1; Pinva = 0.2558; Shape = 0.6827). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 2 in Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Figure 2: Photographs of Oscheius onirici female. (A) Entire body (arrow showing vulva). (B) Pharyngeal region (arrow showing excretory pore). (C) Head region. (D, E) Lateral view of vulva region (arrow showing vulva). (F) Lateral view of tail region (arrow showing anus). (G) Basal bulb (arrow showing excretory pore).

Figure 4 in Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

Figure 4: Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using 28S D2/D3 extension region of 28 rDNA gene. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.

Figure 4 in Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Figure 4: Bayesian consensus tree inferred from 18S under GTR+I+G model (−ln L = 5789.8442; AIC = 11599.6885; freqA = 0.2657; freqC = 0.202; freqG = 0.258; freqT = 0.2743; R(a) = 0.9275; R(b) = 3.6026; R(c) = 2.6802; R(d) = 0.6926; R(e) = 6.3237; R(f) = 1; Pinva = 0.4134; Shape = 0.6825). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 3 in Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Figure 3: Scanning electron microscope photographs of Oscheius onirici female. (A) Lip region en-face view showing one amphideal aperture (am), six labial sensilla (ls) and two cephalic sensilla (cs). (B) Excrotory pore. (C) Ventral view of vulva. (D) Lateral lines. (E) Entire body lateral view (arrow showing vulva). (F) Esophageal region (arrow showing excretory pore). (G) Lateral field showing lateral lines. (H) Tail region ventral view (Top arrow showing four bacteria, middle arrow showing anus, two bottom arrows showing phasmids).

Figure 6 in Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Figure 6: Bayesian consensus tree inferred from ITS under TVM+I model (−ln L = 9235.373; AIC = 18488.7461; freqA = 0.2255; freqC = 0.2115; freqG = 0.2393; freqT = 0.3237; R(a) = 1.3757; R(b) = 3.2802; R(c) = 1.847; R(d) = 1.2014; R(e) = 3.2802; R(f) = 1; Pinva = 0.1375; Shape = 1.5896). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 1 in Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Figure 1: Oscheius onirici female. (A) Entire body. (B) Pharyngeal region. (C) Lateral view of vulva region and lateral field. (D) Schematic representation of En-face view of lip region. (E) Lateral view of tail region.

Figure 1 in Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

Figure 1: Oscheius indicus n. sp. (A) Entire male; (B) Entire female; (C) Pharyngeal region; (D) Anterior region; (E) Female reproductive system; (F) Male posterior region showing spicules and gubernaculum; (G) Male tail in dorso-ventral view (genital papillae and bursa); (H) Female posterior region.

Figure 2 in Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

Figure 2: Oscheius indicus n. sp. (A, B) Anterior region showing stoma; (C) Posterior pharynx.; (D) Genital papillae; (E) Anterior pharynx; (F) Vulva; (G) Male posterior region; (H) Female posterior region; (L) Lateral lines. (Scale bar: 20 µm).

Figure 3 in Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

Figure 3: Oscheius indicus n. sp. (A) Enface view showing six labial papillae (a to f), (B) Crochet needle-shaped spicules (C) Male posterior region showing genital papillae (GP1 to GP9), (D) Female posterior region showing anal opening (Scale bar: A = 2 µm; B & C = 10 µm, D = 5 µm).

Figure 7 in Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Figure 7: The mortality rates for different insect types treated with either an Oscheius onirici population, or a control treatment of two milliliters of distilled water. (B) The average number of days that it took for a larva to die after exposure to the treatment. Insects were treated with either an Oscheius onirici population, or a control treatment of two milliliters of distilled water.

FIGURE 5 in Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave

FIGURE 5. Phylogenetic trees of ITS containing region of Oscheius onirici sp. n. and the closest species. Sequences were analysed using Maximum Likelihood method. Numbers at nodes indicate bootstrap values.

FIGURE 6 in Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave

FIGURE 6. Phylogenetic trees of partial D2-D3 expansion domains of 28S rRNA gene of Oscheius onirici sp. n. and the closest species. Sequences were analysed using Maximum Likelihood method. Numbers at nodes indicate bootstrap values

FIGURE 2 in Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave

FIGURE 2. Light micrographs of Oscheius onirici sp. n. A: Female pharyngeal region; B: Female anterior part of intestine, showing bacteria (bracket); C: Female tail; D: Vulval region, with embryonated eggs in the uterus; E: Ventral view of male tail; F: Lateral view of male tail; G: Male tail showing bursal rays arrangement; H: Female lateral field at midbody; arrowed is a discontinuous fifth line, occasionally visible in some of the specimens; I: Female anterior genital tract showing the spermatheca (bracket). (Scale bars = 10 Μm).

FIGURE 4 in Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave

FIGURE 4. Restriction fragments of amplified ITS containing region of Oscheius onirici sp. n. M:100 bp ladder, B: BamHI, D: DdeI, R: RsaI, Al: AluI, A: Ava II, H: HinfI.

FIGURE 3 in Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave

FIGURE 3. Scanning electron micrographs of Oscheius onirici sp. n. (A, B, C: Juvenile; D, E: Female): A: Entire body; B: Tail; C: Lateral field at mid-body; D, E: Lip region, showing lips with their labial sensilla (D) and amphidial aperture (E, arrowed). (Scale bars: A = 50 Μm; B, C = 5 Μm; D, E = 2 Μm).

FIGURE 7 in Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave

FIGURE 7. Phylogenetic trees of partial 18S rRNA gene of Oscheius onirici sp. n. and the closest species. Sequences were analysed using Maximum Likelihood method. Numbers at nodes indicate bootstrap values.

FIGURE 1 in Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave

FIGURE 1. Oscheius onirici sp. n. A–C: Entire body of female (A), male (B) and dauer juvenile (C); D: Female pharyngeal region; E: Stoma; F: Male pharyngeal region; G: Female tail region; H, I: Male tail in ventral and lateral view, respectively; J: Morphology of male spicule and gubernaculum; K: Female vulval region.