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Dataset results
361 results for “Osteoblasts”
Sclerostin blockade inhibits bone resorption through PDGF receptor signaling in osteoblast lineage cells
<p>While sclerostin-neutralizing antibodies (Scl-Ab) transiently stimulate bone formation by activating Wnt signaling in osteoblast lineage cells, they exert sustained inhibition of bone resorption, suggesting an alternate signaling pathway by which Scl-Ab control osteoclast activity. Since sclerostin can activate platelet-derived growth factor receptors (PDGFRs) in osteoblast lineage cells in vitro and PDGFR signaling in these cells induces bone resorption through M-CSF secretion, we hypothesized that the prolonged anti-catabolic effect of Scl-Ab could result from PDGFR inhibition. We show here that inhibition of PDGFR signaling in osteoblast lineage cells is sufficient and necessary to mediate prolonged Scl-Ab effect on M-CSF secretion and osteoclast activity in mice. Indeed, sclerostin co-activates PDGFRs independently of Wnt/β-catenin signaling inhibition, by forming a ternary complex with LRP6 and PDGFRs in pre-osteoblasts. In turn, Scl-Ab prevents sclerostin-mediated co-activation of PDGFR signaling and consequent M-CSF up-regulation in pre-osteoblast cultures, thereby inhibiting osteoclast activity in pre-osteoblast/osteoclast co-culture assays. These results provide a new potential mechanism explaining the dissociation between anabolic and anti-resorptive effects of long-term Scl-Ab.</p>
GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation
<p>Osteoporosis is a common systemic skeletal disorder resulting in bone fragility and increased fracture risk. It is still necessary to explore its detailed mechanisms and identify novel targets for the treatment of osteoporosis. Previously, we found that an lncRNA named GAS5 in humans could negatively regulate lipoblast/adipocyte differentiation. However, it is still unclear whether GAS5 affects osteoblast differentiation and whether GAS5 is associated with osteoporosis. Our current research found that GAS5 was decreased in the bones and BMSCs, a major origin of osteoblast, in osteoporosis patients. Mechanistically, GAS5 promotes osteoblast differentiation by interacting with UPF1 to degrade Smad7 mRNA. Moreover, a decreased bone mass and impaired bone repair ability were observed in <em>Gas5</em> heterozygous mice, manifesting in osteoporosis. The systemic supplement of <em>Gas5</em>-overexpressing adenoviruses significantly ameliorated bone loss in an osteoporosis mouse model. In conclusion, GAS5 promotes osteoblast differentiation by targeting the UPF1/Smad7 axis and protects against osteoporosis.</p>
Data_Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts
<p>This publication contains the original data supporting our results concerning the application of biphasic pulsed electrical stimulation on MG-63 cells. We aim to discriminate between effects of the electric field and electrochemically conditioned medium. The dataset consists of the original output files, excel sheets to consolidate the information for the experiments as well as Readme.txt files with most important information concerning the data. For additional information on the material and methods as well as results see the corresponding research article.</p>
Supplemental data for: Decreasing miR-433-3p activity in the osteoblast lineage blunts glucocorticoid-mediated bone loss
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GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation
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Dataser from paper "Proliferation of osteoblast precursor cells on the surface of TiO2 nanowires anodically grown on a -type biomedical titanium alloy"
<p>Dataser from paper "Proliferation of osteoblast precursor cells on the surface of TiO2 nanowires anodically grown on a -type biomedical titanium alloy":</p> <p>- <strong>Contact Angle: </strong>Images and measurements.</p> <p><strong>- Fluorescence Microscopy Images:</strong> Images.</p> <p><strong>- MTT and pixel counting:</strong> Measurements.</p> <p> </p> <p> </p> <p> </p> <p> </p> <p> </p> <p> </p>
Osteolitic vs Osteoblastic metastatic lesion: Computational modeling of fracture risk in the human vertebra after screws fixation procedure
<p>Metastatic lesions compromise the mechanical integrity of vertebrae, increasing the fracture risk. Screwfixation is usually performed to guarantee spinal stability and prevent dramatic fracture events. Accordingly, predicting the overall mechanical response in such conditions is critical to planning and optimizing the surgical treatment. This work proposes an image-basedfinite element computational approach describing the mechanical behavior of a patient-specific instrumented metastatic vertebra by assessing the effect of lesion size, location, type and shape on the fracture load and fracture patterns under physiological loading conditions. A specific constitutive model for the metastasis is integrated to account for the effect of the diseased tissue on the bone material properties. Computational results demonstrate that size, location, and type of metastasis significantly affect the overall vertebral mechanical response, and suggest better account these parameters in estimating the fracture risk. Combining multiple osteolytic lesions to account for irregular shape of the overall metastatic tissue has a not significant effect on fracture load of vertebra macroscopically. In addition, the combination of loading mode and metastasis type is shown for the first time as a critical modeling parameter in determining the fracture risk. The proposed computational approach moves towards defining a clinically integrated tool to improve the management of metastatic vertebrae and quantitatively evaluate fracture risk.</p>
Connexin 43 Modulation in Human Chondrocytes, Osteoblasts and Cartilage Explants: Implications for Inflammatory Joint Disorders
<p>Dataset of the scientific publication "Connexin 43 Modulation in Human Chondrocytes, Osteoblasts and Cartilage Explants: Implications for Inflammatory Joint Disorders"</p>
Study on Autologous Osteoblastic Cells Implantation to Early Stage Osteonecrosis of the Femoral Head
ClinicalTrials.gov study NCT01529008. IPD Sharing: Not stated. Countries: 0. Publications: 1.
Phase 1/2a Study on Allogeneic Osteoblastic Cells Implantation in Delayed-Union Fractures
ClinicalTrials.gov study NCT02020590. IPD Sharing: Not stated. Countries: 0. Publications: 1.
Def6 regulates endogenous type-I interferon responses in osteoblasts and suppresses osteogenesis
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Data from: Micro/nano-hierarchical structured TiO2 coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation
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Transcription profiling of mouse osteoblasts under static vs simulated microgravity
The total RNA was extracted from 2T3 pre-osteoblast cells exposed to static or simulated microgravity (Rotating Wall Vessel) conditions. The RNA was then sent to Affymetrix microarray core facility at Baylor College of Medicine (Houston TX) for microarray analysis.
The Role of PTHrP in Osteoblast Response to Microgravity: Implications for Osteoporosis Development.
Prolonged skeletal unloading through bedrest results in bone loss similar to that observed in elderly osteoporotic patients but with an accelerated timeframe. This rapid effect on weight-bearing bones is also observed in astronauts who lose up to 2% of their bone mass per month spent in Space. Despite important implications for Spaceflight travellers and bedridden patients on Earth the exact mechanisms involved in disuse osteoporosis have not been elucidated. Parathyroid hormone-related protein (PTHrP) regulates many physiological processes including skeletal development and has been proposed as a gravisensor. To investigate the role of PTHrP in microgravity-induced bone loss trabecular osteoblasts (TOs) from Pthrp+/+ and -/- mice were exposed to simulated microgravity for 6 days. Viability of TOs decreased in inverse proportion to PTHrP expression levels. Microarray analysis of Pthrp+/+ TOs after 6 days at 0g revealed expression changes in genes encoding prolactins,apoptosis and survival molecules bone metabolism and extra-cellular matrix composition proteins chemokines IGF family and Wnt-related signalling molecules. Importantly 88% of 0g-induced expression changes in Pthrp+/+ cells overlap those observed in Pthrp-/- cells in normal gravity. Pulsatile treatment with PTHrP1-36 peptide during microgravity exposure reversed a large proportion of 0g-induced changes in Pthrp+/+ TOs. Our results confirm PTHrP efficacy as an anabolic agent to prevent microgravity-induced cell death in TOs. Total RNA samples extracted from Pthrp+/+and -/- trabecular osteoblasts (TOs) exposed for 6 days to simulated 0g in Synthecon rotating cell or left 6 days in culture at 1g. Cells had either been treated with a pulsatile treatment (2 h/day) of PTHrP1-36 peptide (10-8M) or received a change in growth medium. In total: 8 different conditions with 2 replicates each i.e. Pthrp+/+ TOs at 0g or 1g with or without PTHrP1-36 treatment and Pthrp-/- TOs at 0g or 1 g,with or without PTHrP1-36 treatment.
RNA seq after RNA pol III manipulation by Brf1 and Maf1 knockdown, Maf1 overexpression and ML60218 treatment in ST2 cells before, and during differentiation into osteoblasts
GEO Series GSE203308. Mus musculus. 48 samples. Type: Expression profiling by high throughput sequencing.
The Osteoblast to Osteocyte Transition: Epigenetic Changes and Response to the Vitamin D3 Hormone [RNA-seq]
GEO Series GSE54783. Mus musculus. 30 samples. Type: Expression profiling by high throughput sequencing.
Osteoblastic response in human bone marrow stromal cells
GEO Series GSE48907. Homo sapiens. 6 samples. Type: Expression profiling by array.
Endothelial-to-osteoblast transition in normal mouse bone development and postnatal growth
GEO Series GSE207839. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
H3.3G34W promotes growth and impedes differentiation of osteoblast-like mesenchymal progenitors in Giant Cell Tumour of Bone [RNA-Seq]
GEO Series GSE149209. Homo sapiens. 38 samples. Type: Expression profiling by high throughput sequencing.
LCM-RNA-Seq data obtained from chick HH36 chondrocytes and osteoblasts
GEO Series GSE186980. Gallus gallus. 20 samples. Type: Expression profiling by high throughput sequencing.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.