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23 results for “PCR-RFLP”
Figure 4 in PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan
Figure 4. Prevalence of six different sub-allele types of Pvmsp-3β (A1-A3, B1-B2 and C1) based on PCR-RFLP.
Figure 2 in PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan
Figure 2. Prevalence of nine different sub-allele types of Pvmsp- 3α(A1-A4), (B1-B3), C1 and D are the nine different alleles from PCR-RFLP.
Figure 3 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 3. Restriction patterns of HinfI inferred from D-loop digestion (M: Marker–100bp DNA Ladder, 1. Ordu, 2. Trabzon, 3. Rize, 4. Artvin, 5–6. Erzincan, 7–8. Kahramanmaraş, 9. Adıyaman, 10–11. Adana, 12. Muğla, 13. Burdur, 14. Konya, 15. Antalya, 16. Mersin, 17. Kastamonu, 18. Zonguldak, 19. Düzce, 20. Balıkesir, 21. İzmir, 22. Aydın, 23. A. uralensis, 24. A. witherbyi, 25. D-loop PCR products).
Figure 2 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 2. Restriction patterns of MboI, HaeIII, and RsaI inferred from cytb digestion (M: Marker–100bp DNA Ladder, 1. Ordu, 2. Trabzon, 3. Rize, 4. Artvin, 5. Erzincan, 6. Kahramanmaraş, 7. Adıyaman, 8. Adana, 9. Muğla, 10. Burdur, 11. Konya, 12. Antalya, 13. Mersin, 14. Kastamonu, 15. Zonguldak, 16. Düzce, 17. Balıkesir, 18. İzmir, 19. Aydın, 20. A. uralensis, 21. A. witherbyi, 22. Cytb PCR product).
Figure 5 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 5. PCoA analysis of A. mystacinus clades. The scatter plot is of the scores of three principal eigenvalues inferred from NTSYS software. Each scatter point represents a specimen of A. mystacinus.
Figure 1 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 1. Sampling localities of A. mystacinus specimens. Table 2. Restriction enzymes and their digestion sites with reaction procedures.
Figure 2 in Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method
Figure 2. Enzyme digestion results of exons 4 and 5 of the GH gene (M: 50-bp DNA ladder; 1–5 and 7: LL genotype, 6: LV genotype).
Figure 4 in Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method
Figure 4. Enzyme digestion results of the exon 10 region of the GHR gene (M: 50-bp DNA ladder, A: AG genotype, B: AA genotype).
Fig. 2. The PCR products identified within the 18S in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation
Fig. 2. The PCR products identified within the 18S rRNA of Eimeria bovis following digestion with two restriction endonucleases: AluI recognising AG∧CT and Hin1II recognising CATG∧. M1: GeneRuler 100 bp Plus DNA Ladder (Thermo Fisher Scientific); M2: GeneRuler 50bp DNA Ladder (Thermo Fisher Scientific); lane 1: European bison colon wall tissue; lane 2: European bison colon wall tissue after digestion; lane 3: E. bovis oocysts of European bison; lane 4: E. bovis oocysts of European bison after digestion.
Fig. 3 in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation
Fig. 3. The virtual double digestion of the 18S rRNA gene of eimerians infecting the large intestine of the European bison with the restriction enzymes Mval (BstNI) recognising CC∧WGG, and KpnI recognising GGTAC∧C, simulated with SnapGene version 5.0.6 (GSL Biotech LLC); M: GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific). (A) A three-band pattern for E. bovis (20 bp, 210 bp, 343 bp). (B) A four-band pattern for E, zuernii (20 bp, 100 bp, 210 bp, 242 bp). (C) A two-band pattern for E. alabamensis (212 bp, 362 bp).
Fig. 1 in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation
Fig. 1. Histopathological lesions associated with endogenous stages of Eimeria spp. in sections of the ileum and colon of European bison (H-E staining). (A) Shortening and blunting of the intestinal villi of the ileum with diffuse infiltration of mononuclear inflammatory cells within the lamina propria, edematous stroma, dilated crypt containing necrotic debris (arrow), and atrophy of submucosal lymphoid follicles (× 20 magnification). (B) Schizonts and degenerating merozoites in the crypt lumen of the colon (arrows); immature macrogamont with a central nucleus (arrowhead) (× 1000 magnification). (C) Immature microgamonts in the epithelial cells of the colon crypt (arrows) (× 400 magnification). (D) Mature microgamont in the epithelial cells of the colon crypt (arrow) (× 1000 magnification). (E) Gametogonic stages of Eimeria development in the epithelial cells of the colon. Microgamont with peripheral microgames (arrowhead), (a) nearly mature microgamonts, (b) macrogamont with eosinophilic wall-forming bodies, (c) early oocyst (× 400 magnification). (F) Mature macrogamont in the epithelial cells of the cecum (arrow) (× 1000 magnification).
Figure 1 in PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan
Figure 1. Prevalence of four different alleles of Pvmsp-3α (A, B, C and D) from PCR-RFLP
Figure 3 in PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan
Figure 3. Prevalence of three different alleles of Pvmsp-3β (A, B, C) from PCR-RFLP.
Figure 4 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 4. UPGMA dendrogram of the composite data by combining cytb and D-loop regions.
Figure 1 in Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method
Figure 1. PCR products of exons 4 and 5 of the GH gene (428-bp band, M: 100-bp DNA ladder).
Data from: Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae
The fruit fly Drosophila melanogaster is a model organism to study several aspects of metazoan biology. Most of the work has been conducted in adult fruit flies, including laboratory and field-derived specimens, but Drosophila melanogaster larvae recently became a valuable model to better understand animal physiology, development or host-microbe interactions. While adult flies can be easily assigned to a given Drosophila species based on morphological characteristics, such visual identification is more intricate at the larval stage. This could explain the limited number of studies focusing on larvae, especially field-derived samples. Here, we developed a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) assay that discriminates D. melanogaster from other ecologically relevant Drosophila species at the larval stage. The method, which targets the cytochrome oxidase I (COI) gene, was validated using laboratory-derived larvae from seven D. melanogaster populations originating from different geographic areas as well as six Drosophila species. We further validated this PCR-RFLP assay in a natural context, by identifying wild larvae collected in two locations in France. Notably, among all PCR-RFLP profiles that matched the D. melanogaster species, 100% were correctly identified, as confirmed by COI sequencing. In summary, our work provides a rapid, simple and accurate molecular tool to identify D. melanogaster from field-collected larvae.
FIGURE 2 in Identification of Planococcus ficus and Planococcus citri (Hemiptera: Pseudococcidae) by PCR-RFLP of COI gene
FIGURE 2. RFLP analysis with HinfI restriction enzyme of COI gene. Digested products were separated on 2% agarose gels stained with ethidium bromide. Lanes: 1-8, Pl citri; 9-13, Pl ficus; M, 100 bp ladder.
FIGURE 1 in Identification of Planococcus ficus and Planococcus citri (Hemiptera: Pseudococcidae) by PCR-RFLP of COI gene
FIGURE 1. COI gene restriction patterns of Ps. longispinus (L), Pl. citri (C) and Pl. ficus (F) generated by BspPI, HinfI and SspI.
Data from: Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae
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Sex identification PCR-RFLP assay tested in eight species of Sebastes rockfish
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