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5 results for “Pacific halibut”

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dryad36/100

Assessing spawning behavior at the Northern latitudinal extreme of Pacific halibut

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publicApr 2025View details →
dryad32/100

d13C and d15N of Pacific halibut skeletal muscle tissue from the Gulf of Alaska, 2018

<p>In 2018, white muscle samples were collected from the lateral musculature of a random sample of up to 4 fish from each survey station of the International Pacific Halibut Commission's Fishery Independent Setline Survey (total n = 1653). Muscle samples were stored at -20°C, thawed, oven-dried to a constant mass at 42°C, and ground to a fine powder. Approximately 0.5 to 1.0 mg of each powdered sample was loaded into a tin capsule for stable isotope analysis. d<sup>13</sup>C and d<sup>15</sup>N values were determined using a Carlo Erba 1110 Elemental Analyzer (Carlo Erba Reagents, CE Instruments, ThermoQuest Italia S.p.A. Milan, Italy) coupled to a Thermo Delta Plus XP IRMS (Thermo Finnigan, Bremen, German) at the University of Wyoming's Stable Isotope Facility (Laramie, Wyoming, USA). Long-term analyses of quality control standards have yielded precisions of 0.3‰ for d<sup>13</sup>C and 0.4‰ for d<sup>15</sup>N. Stable isotope data are presented in ‰ relative to Pee Dee Belemnite for <sup>13</sup>C and atmospheric nitrogen for <sup>15</sup>N.</p>

opencc-zeroMay 2024View details →
dryad32/100

d13C and d15N of Pacific halibut skeletal muscle tissue from the Gulf of Alaska, 2018

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publicMay 2024View details →
dryad28/100

Data from: Identification of genomic regions associated with sex in Pacific halibut

Understanding and identifying the genetic mechanisms responsible for sex-determination are important for species management, particularly in exploited fishes where sex biased harvest could have implications on population dynamics and long-term persistence. The Pacific halibut (Hippoglossus stenolepis) supports important fisheries in the North Pacific Ocean. The proportion of each sex in the annual harvest is currently estimated using growth curves, but genetic techniques may provide a more accurate method. We used restriction-site associated DNA (RAD) sequencing to identify RAD-tags that were linked to genetic sex, based on differentiation (FST) between the sexes. Identified RAD-tags were aligned to the Atlantic halibut (Hippoglossus hippoglossus) linkage map, the turbot (Scophthalmus maximus) genome, and the half-smooth tongue sole (Cynoglossus semilaevis) genome to identify genomic regions that may be involved in sex determination. In total, 56 RAD-tags (70 single nucleotide polymorphisms) were linked to sex, and three RAD-tags were identified in only females. Sex-linked loci aligned to three linkage groups in the Atlantic halibut (LG07: 7 loci, LG15: 1 locus, and LG24: 1 locus), three chromosomes in the turbot (LG12: 13 loci, LG01: 1 locus, and LG05: 1 locus), and one chromosome in the half-smooth tongue sole (ChrZ: 9 loci). Results add support to the hypothesis that Pacific halibut genetic sex is determined in a ZW system. Two sex-linked loci were further developed into sex identification assays, and their efficacy was tested on individuals that had been morphologically sexed. The accuracy of each assay on its own was 97.5% compared to morphological sex.

opencc-zeroDec 2016View details →
dryad28/100

Data from: Identification of genomic regions associated with sex in Pacific halibut

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publicNov 2017View details →

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