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Fig. 4 in The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae

Fig. 4. Histological sections of Pagrus pagrus larvae, indicators of long term nutritional condition. Sagital section of 4 µm thick stained with Harris's hematoxylin and eosin counterstain (H-E). (a-c) nervous system, (d-f) cartilage, (g-i) muscle, (j-l) notochord; left = fed larvae, middle = delayed fed larvae and right = starved. hsn: heavily stained nucleus, ics: inter-cellular space, nc: notochord contraction, pn: prominent nucleus. Scale bar indicates 10 µm.

Fig. 1 in The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae

Fig. 1. Mean values of morphometrical variables and standard error against larval age expressed as days after hatching (DAH) of Pagrus pagrus larvae from the different feeding treatments. BDA: body depth at the anus, ED: eye diameter, HD: head depth, SL: standard length. T0: before the experiment begins, T1: without food, T2: delayed feeding and T3: fed. Arrows indicate the moment when food was supplied to larvae in T3 (black) and T2 (dark grey). Initial condition of larvae (T0, white squares) was also included in order to allow comparisons.

Fig. 7 in The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae

Fig. 7. Mean histological condition index (HCI) and standard error for Pagrus pagrus larvae calculated employing short or long term tissues and a mean value employing all tissues against larval age expressed as days after hatching (DAH) from the feeding treatments, a: without food (T1); b: delayed feeding (T2); c: fed (T3).

Fig. 5 in The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae

Fig. 5. Mean histological condition index (HCI) and standard error for Pagrus pagrus larvae from the feeding treatments against larval age expressed as days after hatching (DAH). Different lowercase letters indicate significant differences (p<0.05) among feeding treatments, after one way ANOVA followed by post-hoc Tukey's test. Different capital letters indicate significant differences (p<0.05) among feeding treatments, after Kruskal Wallis test followed by multiple comparisons. T0: before the experiment begins, T1: without food, T2: delayed feeding and T3: fed.

Fig. 6 in The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae

Fig. 6. Mean histological condition index (HCI) and standard error for Pagrus pagrus larvae from the feeding treatments calculated employing short or long term tissues and a mean value employing all tissues. Different letters indicate significant differences (p<0.05) among feeding treatments, after one way ANOVA followed by post-hoc Tukey's test. T1: without food, T2: delayed feeding and T3: fed, S: short term HCI, M: mean HCI, L: long term HCI.

Fig. 2 in The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae

Fig. 2. Scatterplot of PC2 on PC1 for Pagrus pagrus larvae from the feeding treatments. a: PCA based upon normalized morphometrical variables; b: PCA based upon normalized morphometrical variables and observations grouped by feeding treatments. Correlations between the variables also showed. BDA: body depth at the anus, ED: eye diameter, HD: head depth. T1: without food, T2: delayed feeding and T3: fed. –N indicates normalized morphometrical variables.

FIG. 4. — Lamellodiscus sigillatus n in Lamellodiscus (Plathelminthes, Monogenea, Diplectanidae) nouveaux parasites branchiaux des poissons marins du genre Pagrus (Teleostei, Sparidae)

FIG. 4. — Lamellodiscus sigillatus n. sp.; A, hapteur en vue ventrale; B, barre transversale latérale dorsale; C, barre transversale médiane ventrale; D, gripus ventral; E, gripus dorsal; F, appareil copulateur mâle. Échelle: A, 100 µm; B-F, 50 µm.

FIG. 3. — Lamellodiscus sarculus n in Lamellodiscus (Plathelminthes, Monogenea, Diplectanidae) nouveaux parasites branchiaux des poissons marins du genre Pagrus (Teleostei, Sparidae)

FIG. 3. — Lamellodiscus sarculus n. sp., hapteur en vue ventrale montrant la disposition des uncinili et la position des glandes postérieures. Abréviations: I-VII, numéro des uncinuli. Échelle: 100 µm.

FIG. 5. — Lamellodiscus rastellus n in Lamellodiscus (Plathelminthes, Monogenea, Diplectanidae) nouveaux parasites branchiaux des poissons marins du genre Pagrus (Teleostei, Sparidae)

FIG. 5. — Lamellodiscus rastellus n. sp.; A, barre transversale latérale dorsale; B, barre transversale médiane ventrale; C, gripus dorsal; D, gripus ventral; E, appareil copulateur mâle. Échelle: 50 µm.

FIG. 2. — Lamellodiscus sarculus n in Lamellodiscus (Plathelminthes, Monogenea, Diplectanidae) nouveaux parasites branchiaux des poissons marins du genre Pagrus (Teleostei, Sparidae)

FIG. 2. — Lamellodiscus sarculus n. sp.; A, animal in toto vue ventrale; B, barre transversale latérale dorsale; C, barre transversale médiane ventrale; D, gripus ventral; E, gripus dorsal; F, appareil copulateur mâle. Abréviations: ac, appareil copulateur mâle; def, déférent; gpl, glande postérieure latérale; gpm, glande postérieure médiane; oot, ootype; ov, ovaire; ph, pharynx; ps, pseudospermatophore; rpr, réservoir prostatique; rs, réceptacle séminal; test, testicule; vg, vagin; vit, vitellogènes; vs, vésicule séminale. Échelle: A, 200 µm; B-F, 50 µm.

FIG. 1 in Lamellodiscus (Plathelminthes, Monogenea, Diplectanidae) nouveaux parasites branchiaux des poissons marins du genre Pagrus (Teleostei, Sparidae)

FIG. 1. — Mensurations et nomenclature utilisées pour l'étude des Lamellodiscus; A, pièces sclérifiées du hapteur; B, mensurations du gripus dorsal; C, mensurations du gripus ventral; D, pièces copulatrices du type « lyre »; E, lamellodisque du groupe « ignoratus »; F, lamellodisque du groupe « elegans ». Abréviations: a, axial; l, latéral; Li, longueur de la barre transversale dorsale; Lm, longueur de la barre transversale ventrale; x, longueur.

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Fig. 3 in The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae

Fig. 3. Histological sections of Pagrus pagrus larvae, indicators of short term nutritional condition. Sagital section of 4 µm thick stained with Harris's hematoxylin and eosin counterstain (H-E). (a-c) digestive duct, (d-f) pancreas, (g-i) liver; left = fed larvae, middle = delayed fed lavae and right = starved larvae. bb: brush border, dbb: distinct brush border, dt: dissociated tissue, enp: endocrine pancreas, exp: exocrine pancreas, hsn: heavily stained nucleus, pn: prominent nucleus, se: shrunken enterocytes, we: wide enterocytes. Scale bar indicates 10 µm.