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Fig. 4 in Integrative description of Paramacrobiotus bengalensis sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae), a new limno-terrestrial tardigrade species from the state of West Bengal, India

Fig. 4. Paramacrobiotus bengalensis sp. nov., claws. A. Holotype (ZSI-HQ/GNC/T1/1), claws I with small smooth lunulae. B–C. Paratype (ZSI/TAR_IND/WB/024). B. Claws II with small smooth lunulae. C. Claws III with small smooth lunulae. D. Paratype (ZSI-HQ/GNC/T2/1), claws IV with smooth lunulae, (black filled arrow indicates smooth lunuale for A–D). E–F. Claws II and III without any visible granulation the legs (black filled checkered arrow). Figures assembled from several photos. A–D = PCM; E–F = SEM. Scale bars: A–C = 5 μm; D = 10 μm; E–F = 0.4 μm.

Fig. 7 in Integrative description of Paramacrobiotus bengalensis sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae), a new limno-terrestrial tardigrade species from the state of West Bengal, India

Fig. 7. Paramacrobiotus bengalensis sp. nov., paratype (ZSI/TAR_IND/WB/E001), egg process details. A–B. An egg process under PCM and SEM with weakly visible reticulum. Black empty arrows indicate the cap like structure on the top of the process, black empty checkered arrows indicate the fine reticulation on the process. Scale bars: A = 10 μm; B = 1 μm.

Fig. 6 in Integrative description of Paramacrobiotus bengalensis sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae), a new limno-terrestrial tardigrade species from the state of West Bengal, India

Fig. 6. Paramacrobiotus bengalensis sp. nov., paratype (ZSI/TAR_IND/WB/E001), egg chorion details. A–B. Zoom on egg surface between the processes with clearly visible areolae and cap like structures on processes (SEM), white empty chevron arrows indicate wrinkles inside the areolae. Scale bars: A = 5 μm; B = 2 μm.

Fig. 5 in Integrative description of Paramacrobiotus bengalensis sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae), a new limno-terrestrial tardigrade species from the state of West Bengal, India

Fig. 5. Paramacrobiotus bengalensis sp. nov., paratype (ZSI/TAR_IND/WB/E001). A. Entire egg under SEM. B–F. Eggs seen in PCM under 1000 × magnification. B. Midsection. D–F. Surfaces. Scale bars: A = 3 μm; B–F = 10 μm.

Fig. 3 in Integrative description of Paramacrobiotus bengalensis sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae), a new limno-terrestrial tardigrade species from the state of West Bengal, India

Fig. 3. Paramacrobiotus bengalensis sp. nov., paratypes. A, D. Specimen ZSI-HQ/GNC/T3/1. B–C. Specimen ZSI/TAR_IND/WB/024. A. Buccal apparatus, dorso-ventral projection with dorsal teeth in focus. B–C. Oral cavity armature (B, ventral teeth; C, dorsal teeth). D. Macroplacoids. White unfilled arrow indicate the second band of teeth, unfilled checkered arrow indicate the first band of teeth, white unfilled chevron arrow indicate the third band of teeth, white filled chevron arrow indicates medioventral teeth, a white filled arrow indicates the subterminal constriction in the third macroplacoid. All PCM. Scale bars: A = 10 μm; B–D = 5 μm.

Fig. 2 in Integrative description of Paramacrobiotus bengalensis sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae), a new limno-terrestrial tardigrade species from the state of West Bengal, India

Fig. 2. Paramacrobiotus bengalensis sp. nov. A. Holotype (ZSI-HQ/GNC/T1/1), habitus, dorso-ventral projection. B–C. Paratypes (ZSI-HQ/GNC/T2/1). B. Habitus dorsal projection. C. Scanning electron microphotograph of a whole specimen. Scale bars: A, C = 100 μm; B = 200 μm.

Fig. 8 in Integrative description of Paramacrobiotus bengalensis sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae), a new limno-terrestrial tardigrade species from the state of West Bengal, India

Fig. 8. Phylogeny of Paramacrobiotus Guidetti, Schill, Bertolani, Dandekar & Wolf, 2009 based on the nucleotide sequences of two markers (18S rRNA + COI). A. ML tree. B. BI tree. The numbers at nodes represent Bayesian posterior probability and bootstrap support values. The blue coloured clade represents species from the Paramacrobiotus richtersi morphogroup, while the red coloured clade represents species from the Paramacrobiotus areolatus morphogroup. Both trees have a red colour for the new species. The outgroup is denoted by black triangles. The scale bar reflects the number of substitutions per position.

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Fig. 1 in Integrative description of Paramacrobiotus bengalensis sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae), a new limno-terrestrial tardigrade species from the state of West Bengal, India

Fig. 1. Type locality of the new taxa (marked with red square).

FIGURE 6. A in Eremobiotus ginevrae sp. nov. and Paramacrobiotus pius sp. nov., two new species of Eutardigrada

FIGURE 6. A, Egg of Paramacrobiotus corgatensis. B, Egg of Paramacrobiotus danielisae. C, Egg of Paramacrobiotus peteri. D, Bucco-pharyngeal apparatus and claws of Paramacrobiotus priviterae.

FIGURE 5 in Eremobiotus ginevrae sp. nov. and Paramacrobiotus pius sp. nov., two new species of Eutardigrada

FIGURE 5. Paramacrobiotus pius sp. nov. (holotype): A, Bucco-pharyngeal apparatus. B, Claws of the first pair of legs (arrow indicates the accessory points). C, Claws of the hind legs (arrows indicate the lunules). D, Detail of the egg; the apical short spines of the egg processes are visible (arrow). E, Detail of the egg; the reticular ornamentation of the egg processes is visible (arrows). F, Detail of the egg shell; the sculpture of the areolae is visible (arrows). Scale bar = 10 µm.

FIGURE 7. A in Eremobiotus ginevrae sp. nov. and Paramacrobiotus pius sp. nov., two new species of Eutardigrada

FIGURE 7. A, Detail of the egg of Paramacrobiotus hapukuensis. B, Egg of Paramacrobitus gerlachae. C, Bucco-pharyngeal apparatus of Paramacrobiotus vanescens; the very small microplacoid is visible (arrow). D, Detail of the egg of Paramacrobiotus vanescens.

FIGURE 4 in Eremobiotus ginevrae sp. nov. and Paramacrobiotus pius sp. nov., two new species of Eutardigrada

FIGURE 4. Eremobiotus ovezovae (paratype, slide no. 3809): A, Bucco-pharyngeal apparatus. B, Claws of the second pair of legs. C, Claws of the hind legs. D, Claws of the second pair of legs. E, Claws of the hind legs; accessory points and lunules are visible. Scale bar = 10 µm.

FIGURE 3 in Eremobiotus ginevrae sp. nov. and Paramacrobiotus pius sp. nov., two new species of Eutardigrada

FIGURE 3. Eremobiotus alicatai: A, Bucco-pharyngeal apparatus of the holotype (slide no. 960). B, Claws of the second pair of legs of the holotype. C, Claws of the hind legs of the holotype. The arrow indicates a lunule. D, Claws of the third pair of legs of a paratype (slide no. 2053). Arrow ʻaʼ: indicates an accessory point; arrow ʻbʼ: indicates the long cuticular bar near the base of the internal claw. Scale bar = 10 µm.

FIGURE 1 in Eremobiotus ginevrae sp. nov. and Paramacrobiotus pius sp. nov., two new species of Eutardigrada

FIGURE 1. Eremobiotus ginevrae sp. nov.: A. Bucco-pharyngeal apparatus of the holotype. B, Bucco-pharyngeal apparatus, in lateral view, of a paratype (slide no. 5327). C, Claws of the third pair of legs of the holotype (arrow 'a' indicates a lunule; arrow 'b' the long cuticular bar near the base of the internal claw). D, Claws of the hind legs of the holotype (arrow 'a' indicates an accessory point; arrow 'b' indicates a lunule). Scale bar = 10 µm.

FIGURE 2 in Eremobiotus ginevrae sp. nov. and Paramacrobiotus pius sp. nov., two new species of Eutardigrada

FIGURE 2. Eremobiotus ginevrae sp. nov.: A, Claws of the third pair of legs of a paratype (slide no. 5544); free accessory points are absent. A long cuticular bar (arrow ʽbʼ) is visible near the lunule at the base of internal claw (arrow ʽaʼ). B, Claws of the third pair of legs of a paratype (slide no. 1707); free accessory points are absent (arrow ʽaʼ: indicates a lunule; arrow ʽbʼ: the long cuticular bar near the base of the internal claw). C, Claws of the hind legs of a paratype (slide no. 2389); the well developed accessory points are visible (arrow ʽaʼ); arrow ʽʼbʼ: indicates a lunule. D, Claws of the hind legs of a paratype (slide no. 5327); the accessory points (arrow ʽaʼ) and the lunules (arrow ʽbʼ) are visible. Scale bar = 10 µm.

FIGURE 7C in Description of a model tardigrade Paramacrobiotus metropolitanus sp. nov (Eutardigrada) from Japan with a summary of its life history, reproduction and genomics

FIGURE 7C. Phylogenetic position of the new species based on COI sequences. Values at nodes indicate Bayesian posterior probabilities. Scale bars = substitutions per position.

FIGURE 7B in Description of a model tardigrade Paramacrobiotus metropolitanus sp. nov (Eutardigrada) from Japan with a summary of its life history, reproduction and genomics

FIGURE 7B. Phylogenetic position of the new species based on ITS-2 sequences. Values at nodes indicate Bayesian posterior probabilities. Scale bars = substitutions per position.

FIGURE 7A in Description of a model tardigrade Paramacrobiotus metropolitanus sp. nov (Eutardigrada) from Japan with a summary of its life history, reproduction and genomics

FIGURE 7A. Phylogenetic position of the new species based on the concatenated 18S+28S+ITS-2+COI dataset. Values at nodes indicate Bayesian posterior probabilities. Scale bars = substitutions per position.

FIGURE 6 in Description of a model tardigrade Paramacrobiotus metropolitanus sp. nov (Eutardigrada) from Japan with a summary of its life history, reproduction and genomics

FIGURE 6. Egg characters of Paramacrobiotus metropolitanus sp. nov. Egg surface structures observed with phase-contrast microscopy (A, B) and SEM (C, D, E). The structure of a process is shown in D. E shows smooth areoles on the egg surface. Scale bars = μm.

FIGURE 4 in Description of a model tardigrade Paramacrobiotus metropolitanus sp. nov (Eutardigrada) from Japan with a summary of its life history, reproduction and genomics

FIGURE 4. Morphology of leg IV The leg IV observed with LM (A) and SEM (B, C). white-filled indent arrowhead and white dashed line indicate the granulation pattern. White-filled arrows indicate the accessory points. Scale bars = μm.