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Supplementary material 2 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

In situ fighting behavior of Peinaleopolynoe orphanae sp. nov. observed in the Pescadero Basin, Gulf of California, Mexico

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Figure 2 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 2 Haplotype networks from P. orphanae sp. nov. COI data, with small grey circles representing missing haplotypes A seventeen individuals with elytral color coding B twenty-four individuals with geographic locality coding, with twenty-three from the Pescadero Basin, Gulf of California, Mexico and one from Monterey Canyon, California.

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Figure 6 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 6 In situ photos of the new Peinaleopolynoe spp. A–EPeinaleopolynoe orphanae sp. nov. observed in the Pescadero Basin, Gulf of California, Mexico: C, EPeinaleopolynoe orphanae sp. nov. fighting behavior observed; the everted pharynx is used to bite off pieces of the opponent's elytra FPeinaleopolynoe elvisi sp. nov. holotype SIO-BIC A8488 observed and collected next to a whale bone in the Monterey Canyon, California GPeinaleopolynoe elvisi sp. nov. paratype SIO-BIC A9699 observed and collected on a pig bone deployment from Jaco Scar, Costa Rica HPeinaleopolynoe goffrediae sp. nov. holotype SIO-BIC A5485 observed and collected on a whalefall in the Monterey Canyon, California.

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Figure 15 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 15 Micrographs of P. goffrediae sp. nov. holotype SIO-BIC A5485 A right parapodium from segment 10 B right parapodium from segment 11 C notochaeta D superior neurochaeta (supra-acicular) E inferior neurochaeta (subacicular). Abbreviations: br1, branchiae small group 1 attached to dorsal tubercle; br2, branchiae large group 2 attached near base of notopodium; noc, notochaetae; dc, dorsal cirrus; no, notopodium; snec, superior neurochaetae; ne, neuropodium; neap, neuroacicular process; noap, notoacicular process; vc, ventral cirrus; inec, inferior neurochaetae; el, elytrophore; br, single large group of branchiae on elytrigerous segment. Scale bars: 2 mm (A, B); 15 μm (C, E); 10 μm (D).

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Figure 14 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 14 Macro photos and micrographs of P. goffrediae sp. nov. holotype SIO-BIC A5485 and paratype SIO-BIC A5464 A dorsal view, holotype B ventral view, holotype. Segments 12–15 are marked to indicate the presence of four pairs of papillae C frontal view of proboscis showing papillae and jaws, holotype. Numbers mark the papillae on the dorsal (seven papillae) and ventral (six papillae) surfaces D loose elytron, holotype E dorsal view of anterior, holotype F ventral view of anterior, paratype G left side branchiae on segments 12–15, holotype H ventral papillae on segments 12–15 (four pairs) indicated by white arrows, holotype. Ventral lamellae on segments 16–17 (two pairs) indicated by black arrows, holotype I dorsal view of posterior, holotype J ventral view of posterior, holotype. Abbreviations: XII, segment 12; XIII, segment 13; XIV, segment 14; XV, segment 15; j, jaws; ma, median antenna; la, lateral antenna; pa, palp; dac, dorsal anterior cirrus; vac, ventral anterior cirrus; el, elytrophore; br, single large group of branchiae on elytrigerous segment; noc, notochaetae; dc, dorsal cirrus; br1, branchiae small group 1 attached to dorsal tubercle on cirrigerous segment; br2, branchiae large group 2 attached near base of notopodium on cirrigerous segment; no, notopodium; vc, ventral cirrus; bc, buccal cirrus; ne, neuropodium; dt, dorsal tubercle; anc, anal cirrus. Scale bars: 4 mm (A, B); 2 mm (C–F, H–J); 1 mm (G).

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Supplementary material 1 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figures S1, S2

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Figure 10 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 10 Micrographs of Peinaleopolynoe spp. jaws APeinaleopolynoe orphanae sp. nov. paratype SIO-BIC A9996 BPeinaleopolynoe elvisi sp. nov. holotype SIO-BIC A8488 CPeinaleopolynoe goffrediae sp. nov. holotype SIO-BIC A5485 DPeinaleopolynoe mineoi sp. nov. paratype SIO-BIC A9709. Scale bars: 3 mm (A, C); 2 mm (B, D).

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Figure 11 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 11 Live dorsal views of P. orphanae sp. nov. Arrows indicate elytral bite marks from the fighting behavior A paratype SIO-BIC A10020 with blue elytra B paratype SIO-BIC A10024 with pink elytra C paratype SIO-BIC A6312 with white elytra D paratype SIO-BIC A6166 with black elytra E paratype SIO-BIC A10023 with red elytra. Scale bars: 5 mm (A, B, D, E); 3 mm (C).

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Figure 1 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 1 Maximum likelihood (ML) tree of the combined analysis from six genes (COI, 16S, 18S, 28S, H3, CytB) aligned with MAFFT and then concatenated. Numbers next to nodes are ML bootstrap percentages from RAxML, Bayesian inference (BI) posterior probability, and maximum parsimony (MP) jackknife support values, separated by slashes. Key: * indicates 95% bootstrap/jackknife or greater and 0.95 posterior probability or greater. – indicates the node was not found. Branchiae drawings at terminals indicate presence of arborescent or plicate branchiae; ~ indicates that on segments with two groups of branchiae, the position in T. branchiata is split into anterior and posterior groups, as opposed to upper and lower groups in remaining taxa. The seven paraphyletic groups of Branchinotogluma are highlighted in a yellow-orange gradient.

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Figure 17 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 17 Micrographs of P. mineoi sp. nov. holotype SIO-BIC A10071 A right parapodium from segment 10 B right parapodium from segment 11 C notochaetae D superior neurochaetae (supra-acicular) E inferior neurochaetae (subacicular). Abbreviations: br1, branchiae small group 1 attached to dorsal tubercle; br2, branchiae large group 2 attached near base of notopodium; noc, notochaetae; dc, dorsal cirrus; no, notopodium; snec, superior neurochaetae; ne, neuropodium; neap, neuroacicular process; noap, notoacicular process; vc, ventral cirrus; inec, inferior neurochaetae; el, elytrophore; br, single large group of branchiae on elytrigerous segment. Scale bars: 0.5 mm (A, B); 15 μm (C); 5 μm (D, E).

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Figure 5 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 5 Peinaleopolynoe santacatalina specimens ASIO-BIC A8487, live dorsal view BPeinaleopolynoe santacatalina observed on the Rosebud Whalefall off the coast of San Diego, CA CSIO-BIC A8489, live dorsal view without elytra. Numbers next to the elytrophores indicate the pairs of elytra (ten total) DSIO-BIC A10927, live ventral view. Arrows indicate the four pairs of papillae on segments 12–15. Abbreviations: el, elytrophore; br, single large group of branchiae on elytrigerous segment; br1, branchiae small group 1 attached to dorsal tubercle on cirrigerous segment; br2, branchiae large group 2 attached near base of notopodium on cirrigerous segment. Scale bars: 5 mm (A, C, D); 10 mm (B).

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Figure 16 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 16 Micrographs of P. mineoi sp. nov. holotype SIO-BIC A10071 and paratype SIO-BIC A9709 A dorsal view, holotype B ventral view, holotype. Segments 12–15 are marked to indicate the presence of four pairs of papillae C frontal view of proboscis showing papillae and jaws, paratype. Numbers mark the papillae on the dorsal (seven papillae) and ventral (six papillae) surfaces D loose elytron, holotype E dorsal view of anterior, holotype F ventral view of anterior, holotype G right side branchiae on segments 8–13, holotype H ventral papillae on segments 12–15 (four pairs) indicated by arrows, holotype I dorsal view of posterior, holotype J ventral view of posterior, holotype. Abbreviations: XII, segment 12; XIII, segment 13; XIV, segment 14; XV, segment 15; j, jaws; ma, median antenna; la, lateral antenna; pa, palp; dac, dorsal anterior cirrus; vac, ventral anterior cirrus; el, elytrophore; br, branchiae; nec, neurochaetae; noc, notochaetae; dc, dorsal cirrus; no, notopodium; vc, ventral cirrus; bc, buccal cirrus; ne, neuropodium; dt, dorsal tubercle; anc, anal cirrus. Scale bars: 1 mm (A, B, D); 0.5 mm (C, E–J).

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Figure 4 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 4 Maximum likelihood tree topology from concatenated data (COI, 16S, 18S, 28S, H3, CytB) for Peinaleopolynoe and its sister clade (B. bipapillata and B. sp. nov. 1), with the transformation for A ventral segmental papillae and/or lamellae and B elytral pairs. 'Pie charts' at the nodes represent probabilities for the relevant states.

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Figure 9 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 9 Micrographs of P. orphanae sp. nov. holotype SIO-BIC A6151 A left parapodium from segment 6 B left parapodium from segment 9 C notochaeta D superior neurochaeta (supra-acicular) E inferior neurochaeta (subacicular). Abbreviations: br1, branchiae small group 1 attached to dorsal tubercle; br2, branchiae large group 2 attached near base of notopodium; noc, notochaetae; dc, dorsal cirrus; no, notopodium; snec, superior neurochaetae; ne, neuropodium; neap, neuroacicular process; vc, ventral cirrus; inec, inferior neurochaetae; el, elytrophore; br, single large group of branchiae on elytrigerous segment. Scale bars: 2 mm (A, B); 15 μm (C); 10 μm (D, E).

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Figure 13 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 13 Micrographs of P. elvisi sp. nov. holotype SIO-BIC A8488 A right parapodium from segment 10 B right parapodium from segment 7 C notochaetae D superior neurochaetae (supra-acicular) E inferior neurochaetae (subacicular). Abbreviations: br1, branchiae small group 1 attached to dorsal tubercle; br2, branchiae large group 2 attached near base of notopodium; noc, notochaetae; dc, dorsal cirrus; no, notopodium; snec, superior neurochaetae; ne, neuropodium; neap, neuroacicular process; noap, notoacicular process; vc, ventral cirrus; inec, inferior neurochaetae; el, elytrophore; br, single large group of branchiae on elytrigerous segment. Scale bars: 1 mm (A, B); 15 μm (C); 10 μm (D, E).

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Figure 3 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 3 Haplotype networks from COI data with geographic locality coding; each colored circle represents a single individual APeinaleopolynoe santacatalina network includes four individuals, three from the Rosebud Whalefall off San Diego, California and one from the Del Mar Seeps, California BPeinaleopolynoe mineoi sp. nov. network includes four individuals, three from Mound 12, Costa Rica and one from Mound 11, Costa Rica CPeinaleopolynoe elvisi sp. nov. includes five individuals, two from Jaco Scar, Costa Rica, two from Seamount 1, Costa Rica, and one from the Patrick Whalefall in Monterey Canyon, California. The small black circle represents a missing haplotype.

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Figure 12 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 12 Macro photos and micrographs of P. elvisi sp. nov. holotype SIO-BIC A8488 A dorsal view B ventral view. Segments 12–15 are marked to indicate the presence of four pairs of papillae C frontal view of proboscis showing papillae and jaws. Numbers mark the papillae on the dorsal (six papillae) surface D left elytron from segment 2 E dorsal view of anterior F ventral view of anterior G left side branchiae on segments 7–11 H ventral papillae on segments 12–15 (four pairs) indicated by white arrows. Ventral lamellae on segments 16–17 (two pairs) indicated by black arrows I dorsal view of posterior J ventral view of posterior. Abbreviations: XII, segment 12; XIII, segment 13; XIV, segment 14; XV, segment 15; j, jaws; ma, median antenna; pa, palp; la, lateral antenna; vac, ventral anterior cirrus; el, elytrophore; br, single large group of branchiae on elytrigerous segment; noc, notochaetae; dc, dorsal cirrus; br1, branchiae small group 1 attached to dorsal tubercle on cirrigerous segment; br2, branchiae large group 2 attached near base of notopodium on cirrigerous segment; no, notopodium; nec, neurochaetae; vc, ventral cirrus; ne, neuropodium; dt, dorsal tubercle; anc, anal cirrus. Scale bars: 4 mm (A, B); 0.5 mm (C); 1 mm (D, H–J); 2 mm (E–G).

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Figure 8 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 8 Macro photos and micrographs of P. orphanae sp. nov. holotype SIO-BIC A6151 and paratype SIO-BIC A9996 A dorsal view, holotype B ventral view, holotype. Segments 12–15 are marked to indicate the presence of four pairs of papillae C frontal view of proboscis showing papillae, paratype. Numbers mark the papillae on the dorsal (seven papillae) and ventral (six papillae) surfaces D right elytron from segment 5, holotype E dorsal view of anterior, holotype F ventral view of anterior, holotype G right side branchiae on segments 8–11, holotype H ventral papillae on segments 12–15 (four pairs) indicated by arrows, holotype I dorsal view of posterior, holotype J ventral view of posterior, holotype. Abbreviations: XII, segment 12; XIII, segment 13; XIV, segment 14; XV, segment 15; ma, median antenna; la, lateral antenna; pa, palp; dac, dorsal anterior cirrus; vac, ventral anterior cirrus; el, elytrophore; br, single large group of branchiae on elytrigerous segment; noc, notochaetae; dc, dorsal cirrus; vc, ventral cirrus; bc, buccal cirrus; br1, branchiae small group 1 attached to dorsal tubercle on cirrigerous segment; br2, branchiae large group 2 attached near base of notopodium on cirrigerous segment; no, notopodium; ne, neuropodium; nec, neurochaetae; dt, dorsal tubercle; anc, anal cirrus. Scale bars: 4 mm (A, B); 2 mm (C–J).

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Figure 7 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 7 Live dorsal views of the new Peinaleopolynoe spp. APeinaleopolynoe orphanae sp. nov. holotype SIO-BIC A6151 BPeinaleopolynoe elvisi sp. nov. holotype SIO-BIC A8488 CPeinaleopolynoe goffrediae sp. nov. holotype SIO-BIC A5485 DPeinaleopolynoe mineoi sp. nov. holotype SIO-BIC A10071. Scale bars: 6 mm (A); 8 mm (B, C); 1 mm (D).

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