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Dataset results
16 results for “Peptide mapping”
Data from: Identification and mapping of linear antibody epitopes in human serum albumin using high-density peptide arrays
We have recently developed a high-density photolithographic, peptide array technology with a theoretical upper limit of 2 million different peptides per array of 2 cm2. Here, we have used this to perform complete and exhaustive analyses of linear B cell epitopes of a medium sized protein target using human serum albumin (HSA) as an example. All possible overlapping 15-mers from HSA were synthesized and probed with a commercially available polyclonal rabbit anti-HSA antibody preparation. To allow for identification of even the weakest epitopes and at the same time perform a detailed characterization of key residues involved in antibody binding, the array also included complete single substitution scans (i.e. including each of the 20 common amino acids) at each position of each 15-mer peptide. As specificity controls, all possible 15-mer peptides from bovine serum albumin (BSA) and from rabbit serum albumin (RSA) were included as well. The resulting layout contained more than 200.000 peptide fields and could be synthesized in a single array on a microscope slide. More than 20 linear epitope candidates were identified and characterized at high resolution i.e. identifying which amino acids in which positions were needed, or not needed, for antibody interaction. As expected, moderate cross-reaction with some peptides in BSA was identified whereas no cross-reaction was observed with peptides from RSA. We conclude that high-density peptide microarrays are a very powerful methodology to identify and characterize linear antibody epitopes, and should advance detailed description of individual specificities at the single antibody level as well as serologic analysis at the proteome-wide level.
Peptide mapping images of Ciona brain
<p>Peptide mapping images of the Ciona cerebral ganglion. GIMP format. </p>
Molecular mapping of urinary complement peptides in kidney diseases
<p><strong>Abstract: </strong>Defective complement activation has been associated with various types of kidney disease. This led to the hypothesis that specific urine complement fragments may be associated with kidney disease etiologies, and disease progression may be reflected by changes in these complement fragments. We investigated the occurrence of complement fragments in urine, their association with kidney function and disease etiology in 16,027 subjects, using mass spectrometry based peptidomics data from the Human Urinary Proteome/Peptidome Database. Twenty-three different urinary peptides originating from complement proteins C3, C4 and factor B (CFB) could be identified. Most C3-derived peptides showed inverse association with estimated glomerular filtration rate (eGFR), while the majority of peptides derived from CFB demonstrated positive association with eGFR. Several peptides derived from the complement proteins C3, C4 and CFB were found significantly associated with specific kidney disease etiologies. These peptides may depict disease-specific complement activation and could serve as non-invasive biomarkers to support development of complement interventions through assessing complement activity for patients’ stratification and monitoring of drug impact. Further investigation of these complement peptides may provide additional insight into disease pathophysiology and could possibly guide therapeutic decisions, especially when targeting complement factors.</p>
Data from: Immunoreactive peptide maps of SARS-CoV-2
<p>Serodiagnosis of SARS-CoV-2 infection is impeded by immunological cross-reactivity among the human coronaviruses (HCoVs) SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, 229E, HKU1, and NL63. To study humoral immune responses specific to SARS-CoV-2, it is imperative to identify peptides that may enable discrimination between exposure to SARS-CoV-2 and other HCoVs. We used a high-density peptide microarray and plasma samples collected at two time points from 50 subjects with SARS-CoV-2 infection confirmed by qPCR, samples collected in 2004-2005 from 11 subjects with IgG antibodies to SARS-CoV-1, 11 subjects with IgG antibodies to other seasonal human coronaviruses (HCoV), and 10 healthy human subjects. The peptide microarrays consist of ~172000 12-mer peptide probes spanning whole viral genomes. This dataset consists of reactivity values for individual peptides from all 132 plasma samples for both IgG and IgM. Also made available is the correspondence key that provides a reference for all viral genome sequences that a peptide originated from.</p>
Data from: Immunoreactive peptide maps of SARS-CoV-2
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Data from: Identification and mapping of linear antibody epitopes in human serum albumin using high-density peptide arrays
Open the record for dataset details and reuse information.
Epitope mapping of 11 monoclonal antibodies directed against Neisserial adesin A by peptide microarray
GEO Series GSE98881. Homo sapiens; Neisseria meningitidis serogroup B. 11 samples. Type: Protein profiling by protein array.
Epitope mapping of 5 monoclonal antibodies directed against meningococcal factor H binding protein by peptide microarray
GEO Series GSE98879. Homo sapiens; Neisseria meningitidis serogroup B. 5 samples. Type: Protein profiling by protein array.
Inhibition of Tumor Growth and Metastasis by EMMPRIN Multiple Antigenic Peptide (MAP) Vaccination Is Mediated by Immune Modulation
GEO Series GSE85440. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
Epitope mapping of a monoclonal antibody directed against Neisserial Heparin Binding Antigen by peptide microarray
GEO Series GSE81377. Neisseria meningitidis serogroup B; Mus musculus. 3 samples. Type: Protein profiling by protein array.
Epitope mapping of 4 monoclonal antibodies directed against Neisserial Heparin Binding Antigen by peptide microarray
GEO Series GSE98882. Neisseria meningitidis serogroup B; Homo sapiens. 4 samples. Type: Protein profiling by protein array.
Mapping the anti-cancer activity of α-connexin carboxyl-terminal (aCT1) peptide in resistant HER2+ breast cancer
GEO Series GSE250543. Homo sapiens. 6 samples. Type: Other.
Linear epitope mapping in the E and NS1 proteins of dengue and Zika viruses: prospection of peptides for vaccines and diagnostics GA_DZ_V1
GEO Series GSE235043. Dengue virus; Zika virus; Homo sapiens. 29 samples. Type: Protein profiling by protein array.
Linear epitope mapping in the E and NS1 proteins of dengue and Zika viruses: prospection of peptides for vaccines and diagnostics GA_DZ_V2
GEO Series GSE235044. Homo sapiens; Dengue virus; Zika virus. 64 samples. Type: Protein profiling by protein array.
Linear epitope mapping in the E and NS1 proteins of dengue and Zika viruses: prospection of peptides for vaccines and diagnostics
GEO Series GSE235045. Homo sapiens; Dengue virus; Zika virus. 93 samples. Type: Protein profiling by protein array.
C-type natriuretic peptide regulates endochondral ossification through p38 MAP kinase-dependent pathways_2
GEO Series GSE4481. Mus musculus. 18 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.