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14 results for “Petri dish”
Exploring Cellular Dynamics: A Comprehensive Analysis of SH-SY5Y Cell Growth in Variable Polycarbonate Chambers and Comparative Insights with Standard Petri Dishes
<div> <div> <div> <div> <div> <div> <p>The provided illustrations delineate the proliferation of SH-SY5Y cells within polycarbonate chambers with varying diameters, ranging from 3 mm to 50 mm. HBE and HeLaGFP cells serve as points of comparison. Five microscopic images were captured for each culture plate, one at the central location and four at random sites. To trace the growth trajectory across different chamber sizes, images from various culture days underwent manual binarization using GIMP and/or ImageJ software. The resulting binarized images can be accessed in the 'masks' directories within the zipped files, corresponding to the attached images. Comparative analysis of cell growth within these chambers was conducted against growth observed in standard 60 mm Petri dishes from Corning.</p> <p>For monitoring cell growth, we employed an Olympus phase-contrast microscope (CKX53) equipped with a DLT camera (DLT-Cam PRO 1080 HDMI USB).</p> <div> <div> <div> <p>The file nomenclature employed herein serves to represent the primary experimental conditions under consideration. It comprises a hierarchical structure, encompassing various parameters as follows: cellsName_passageNumber_cellSuspension_measurementDayNumber_chamberDiameter_regionNumber_hOfFluids_number.</p> <p>The cellsNames parameter encapsulates the complete nomenclature of the cells utilized in the experiment.</p> <p>The passageNumber parameter represents the passage number and is encoded as 'pNumber.'</p> <p>The cellSuspension parameter is denoted by distinct codes:</p> <ul> <li>'c1' designates a cell suspension with a concentration of 5x10^5 cells/ml.</li> <li>'c3' signifies a cell suspension with a concentration of 1x10^5 cells/ml.</li> <li>'c4' denotes a cell suspension with a concentration of 5x10^4 cells/ml.</li> </ul> <p>The measurementDayNumber parameter is encoded as 'afterXXXdays,' signifying the number of culture days.</p> <p>The 'd' followed by a number (e.g., d6, d8, d10, d12, d15) indicates the diameter of the chamber in millimeters. In a given series of files (e.g., 1day_d6_1, 1day_d6_2, 1day_d6_3, 1day_d6_4, 1day_d6_5), the trailing numeral (_1, _2, _3, _4, _5) corresponds to a photograph captured in a distinct region of the sample.</p> <p>The hOfFluids parameter is employed to signify the height ('h') of the fluid above the cells in the chamber, expressed in millimeters.</p> <div> <div> <div> <p>In adherence to the aforementioned conventions, every file name serves to distinctly characterize a particular collection of experimental conditions. This practice enhances both the comprehensibility and systematic organization of the data. It is noteworthy that the complete set of parameters may not always be provided; for instance, the height of the column of medium is assumed to be 2 mm unless otherwise specified.</p> </div> </div> </div> </div> </div> </div> <br> <p> </p> </div> </div> </div> </div> </div> </div>
Supplementary material S30: The trend in times intervals of jolting pulses for four mite individuals on various substrates (petri-dish, brood-comb and honeycomb).
<p>Jolt occurrence time intervals with respect to time. Data are shown here for a second and third mite on petri-dish (a, b and c, d respectively), a second mite on brood-comb (e, f) and the mite that produces audible jolting pulses on the empty honeycomb that is referred to in the main text (g, h). Jolt occurrences and time between consecutive instances of jolting are showcased in both linear (a, c, e, g) and logarithmic (b, d, f, h) forms. A change in the colour of the data points is indicative of the mite moving to a new position on the substrate.</p>
Supplementary material S28: Modal response of the petri-dish and accelerometer.
<p>Accelerometer modal response, with petri-dish, petri-dish and lid, and alone. An artificial vibration was driven at a 25% magnitude of the PC maximum output volume with a frequency sweep from 0 to 24kHz, via an electromagnetic shaker, to the accelerometer (green), accelerometer glued to the petri-dish (black) and the accelerometer glued to the petri-dish with the lid placed on (red). These figures demonstrate the linearity of the recording system in both linear and logarithmic scales. A large deviation from linearity is seen at 18kHz, where our signals of interest are seldom seen. The only other discrepancy is seen at 500Hz, where the petri-dish exhibits a resonance. The electromagnetic shaker is incapable of vibrating the accelerometer beyond 21kHz, causing the large drop of response seen at ultra-high frequencies.</p>
Supplementary material S21: Averaged jolting pulses on petri-dish that exhibit lower frequency bandwidths.
<p>The average spectrogram of the ten <em>Varroa</em> jolting pulses registered on the petri-dish that exhibit a mid-frequency bandwidth. The amplitude of acceleration is in logarithmic (to the base 10) where the average highest magnitude is dark red (2.5x10<sup>-2</sup> m/s<sup>2</sup>) and the lowest magnitude is dark blue (forced to be 1/30 of the maximum). The maximum is here forced to be that found in the averaged mite pulse, as this figure is simply to demonstrate the presence of the lower frequency bandwidth. Therefore, the absolute values of the colour-coding are not relevant in this case.</p>
Supplementary material S13: Petri-dish jolting pulse spectra.
<p>Jolting pulse spectra on the petri-dish substrate. The magnitude of acceleration is logarithmic (to the power 10) where the highest magnitude (dark red) is 6.1x10<sup>-4 </sup>m/s<sup>2</sup> and the lowest magnitude (dark blue) is forced to be 1/50 of the maximum to reduce the contribution of meaningless noise. All pulses have been high pass filtered at 300 Hz to remove background noise without interfering with the frequency range where signal originating from the <em>Varroa </em>pulse is seen. </p>
Supplementary material S12: All petri-dish jolting pulses shown as individual spectrograms.
<p>Video showcasing the full collection of 250 petri-dish <em>Varroa</em> jolting pulses. Every pulse in the collection is aligned to the centre of the window and presented in decreasing order of strength. The full breadth of variation can be seen between the jolting pulses when viewing them in this way. The magnitude of acceleration is logarithmic (to the base 10) where the highest magnitude is 2x10<sup>-3 </sup>m/s<sup>2</sup> and the lowest magnitude forced to be 1/40 of the maximum to reduce the contribution of meaningless noise.</p>
Supplementary material S9: Video analysis of Varroa jolting pulses on petri-dish.
<p>Video showing the accelerometer data in spectrogram format and synchronous displacements of the <em>Varroa</em> individual on petri-dish. The excerpt demonstrates approximately 10 seconds of continuous jolting behaviour slowed down ten times for ease of viewing and hearing of the rapid jolting behaviour. The soundtrack of this video, also slowed by a factor ten, results in the ultra-high frequency (23 kHz) jolting pulses to be heard at 2200 Hz. Not all pulses produce an audible signature, but for those that do, the sound could be described as a quiet ‘clinking’ noise. The spectrogram shows acceleration magnitude in logarithmic (to the base 10) scale, with dark red showing the highest magnitude and dark blue as the lowest at 1/70<sup>th</sup> of the maximum. The maximum acceleration magnitude is forced to be that of the <em>Varroa </em>jolting pulses for better viewing. The original video data is shown in panel ‘a’. Panel ‘b’ is a replica of this data, further cropped on the mite and demonstrate simple edge detection by means of the spatial gradient of the pixel intensity. Panel ‘c’ further shows the temporal changes in pixel intensity in two consecutive frames seen in panel ‘b’. When motionless the mite is mostly seen as dark blue in ‘c’, but when moving the pixels flash red. The sum of the pixel intensities in this panel are then displayed as the white line superimposed on the spectrogram data, demonstrating the remarkable synchronicity between video-detected mite displacement and accelerometer trace.</p>
Petri dish effect of hyperparasitoids
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Petri dish
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Medicago truncatula germinated seedlings grown in Petri dishes for 3 days then carry out salt stress
GEO Series GSE13921. Sinorhizobium meliloti; Medicago truncatula; Medicago sativa. 12 samples. Type: Expression profiling by array.
Petri dish
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Petri dish
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Effect of polystyrene petri dishes modified with small intestinal submucosa on the human umbilical cord-derived mesenchymal stem cells
GEO Series GSE249610. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
Supplementary information for "Ultrasonic single beam manipulation of particles and organoids through a petri-dish and a plastic tube using an acoustic field live-visualisation method"
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