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8 results for “Piper malgassicum”
Figure 2 from: Corti E, Palchetti E, Biricolti S, Gori M, Tani C, Squillace A, Pittella A, Papini A, Falsini S (2021) Histochemical observations in Piper malgassicum (Piperaceae) with a special focus on the epidermis. Italian Botanist 12: 29-47. https://doi.org/10.3897/italianbotanist.12.70675
Figure 2 Cross-sections of P. malgassicum leaf, LM images A–D are cross-section of P. malgassicum leaf F, GLM observations with polarized light H, ILM observations using birefringence filter A portion of the leaf lamina B detail of image A C detail of image B D detail of C with stomata E portion of the leaf lamina. Presence of CaOx crystals (white arrows) within cells as well as embedded in vascular bundlecell walls F cross-section of the leaf through midrib. Abundance of CaOx crystals (white arrows) close to the bundles and embedded in the cell walls of xylemelements G presence of CaOx crystals (white arrows) in the lower hypodermis H portion of the lamina. CaOx crystals (white arrows) in the lower hypodermis I cross-section of the leaf through midrib showing abundant CaOx crystals (white arrow) close to the bundles and embedded in the cell walls of xylemelements. Xy: xylem, Gt: glandular trichomes; Ue: upper/adaxial epidermis, Uh: upper hypodermis, Pa: palisade tissue, Sp: spongy tissue, Lh: lower hypodermis, Le: lower/abaxial epidermis, St: stomata.
Figure 5 from: Corti E, Palchetti E, Biricolti S, Gori M, Tani C, Squillace A, Pittella A, Papini A, Falsini S (2021) Histochemical observations in Piper malgassicum (Piperaceae) with a special focus on the epidermis. Italian Botanist 12: 29-47. https://doi.org/10.3897/italianbotanist.12.70675
Figure 5 Stem cross-sections ACaOx (white arrows) in the hypodermis with birefringence filter B detail of A C sudan III–IV positive cuticle positive D sudan III–IV positive lipid droplets in the primary phloem E calcofluor positive (blue fluorescence) hypodermis cell walls F cortex and phloem cells walls staining positively withcalcofluor (blue fluorescence) G xylem vessels of both cortical and medullary bundles resulted positive to phloroglucinol H Detail of G.
Figure 8 from: Corti E, Palchetti E, Biricolti S, Gori M, Tani C, Squillace A, Pittella A, Papini A, Falsini S (2021) Histochemical observations in Piper malgassicum (Piperaceae) with a special focus on the epidermis. Italian Botanist 12: 29-47. https://doi.org/10.3897/italianbotanist.12.70675
Figure 8 LM image of the longitudinal section of female inflorescence stained with toluidine blue A the ovary contains a single ovule and is surmounted by a 2-branched stigma B resin ducts as that in the image (asterisk) could be observed in the fleshy ovary. The stigmas were covered with papillae (arrows) CCaOx crystals deposits were present in the ovary at maturity D female inflorescence spike axis, cross-section. Two concentric circles of vascular bundles, with a lacuna in the middle E vascular bundle. Toluidine blue positive material in the phloem.
Figure 1 from: Corti E, Palchetti E, Biricolti S, Gori M, Tani C, Squillace A, Pittella A, Papini A, Falsini S (2021) Histochemical observations in Piper malgassicum (Piperaceae) with a special focus on the epidermis. Italian Botanist 12: 29-47. https://doi.org/10.3897/italianbotanist.12.70675
Figure 1 Herbarium specimen of P. malgassicumA inflorescence B leaves and infructescences (sample conserved at the Tropical Herbarium of Florence, University of Florence).
Figure 7 from: Corti E, Palchetti E, Biricolti S, Gori M, Tani C, Squillace A, Pittella A, Papini A, Falsini S (2021) Histochemical observations in Piper malgassicum (Piperaceae) with a special focus on the epidermis. Italian Botanist 12: 29-47. https://doi.org/10.3897/italianbotanist.12.70675
Figure 7 Epidermis cell of anther. A medium electron-dense bodies formed inside a plastid is surrounded by in a membrane and a mitochondrion is very close to the external plastid membrane. EDB: electron-dense Body; M: mitochondrion; N: nucleus; P: plastid.
Figure 3 from: Corti E, Palchetti E, Biricolti S, Gori M, Tani C, Squillace A, Pittella A, Papini A, Falsini S (2021) Histochemical observations in Piper malgassicum (Piperaceae) with a special focus on the epidermis. Italian Botanist 12: 29-47. https://doi.org/10.3897/italianbotanist.12.70675
Figure 3 A portion of the leaf lamina in autofluorescence under blue-violet light showing the red fluorescence of chlorophyll and the red fluorescence of the trichome contents B detail of image A. Lipid droplets show green fluorescence C trichomes and cuticles appearing Sudan Black positive D sudan Black stained lipid droplets positively in the spaces between chlorenchyma cells, both in the spongy and the palisade parenchyma (black arrows) E leaf stained with NADI reaction. Positive cells can be observed in the mesophyll (arrows) F lower epidermis and trichome stained with NADI GLM images showing the trichome and cuticle slightly reddish while cutin and suberin resulted stainedbrownish with Sudan III–IV H Fluorescence images with Fluorol Yellow staining revealing the lipids in the cuticleand aggregates in the epidermis and mesophyll I detail of H): trichome stained with FY088. Ld: lipid droplet; Ue: upper/adaxial epidermis, Uh: upper hypodermis, Pa: palisade tissue, Sp: spongy tissue, Lh: lower hypodermis, Le: lower/abaxial epidermis, Gt: glandular trichomes.
Figure 6 from: Corti E, Palchetti E, Biricolti S, Gori M, Tani C, Squillace A, Pittella A, Papini A, Falsini S (2021) Histochemical observations in Piper malgassicum (Piperaceae) with a special focus on the epidermis. Italian Botanist 12: 29-47. https://doi.org/10.3897/italianbotanist.12.70675
Figure 6 P. malgassicum anther A Semithin section stained with toluidine blue showing the shape and structure of the anther. Anther wall (here already dehiscent) formed by 2–3 layers of cells B semithin section showing the microspores surrounded by the tapetum C microspores are in the locule surrounded by the tapetum. Small electron-dense particles (orbicles) are lined along the tapetal cells plasma membrane, while some larger masses can be observed in the locule (black arrow) D anther trichome. Some plastids contain large electron-dense bodies E detail of D The large electron-dense bodies inside the plastid occur together with starch grains F anther epidermis: electron-dense bodies are present in the vacuoles. Some plastids are present with stored starch. Large medium electron dense granular bodies can be found between the vacuole and the external plasma membrane G in a preliminary stage, the electron-dense masses in the vacuole are surrounded by a more electron transparent layer H at a successive stage, the electron-dense bodies occupy a large part of the vacuolar volume. Ga: microspores/male gametophyte; L: locule; sp: tapetum/sporophytic tissue; ep: epidermis; end: endothecium, ml: middle layer; st: secretory tapetum; Mcs: microspore; EDB: electron-dense bodies; Mt: mitochondria; Pa: protein aggregate; Ep: elaioplast; yLPT: young lipotubule: Lpt: lipotubule; V: vacuole; Td: terpenic droplets.
Figure 4 from: Corti E, Palchetti E, Biricolti S, Gori M, Tani C, Squillace A, Pittella A, Papini A, Falsini S (2021) Histochemical observations in Piper malgassicum (Piperaceae) with a special focus on the epidermis. Italian Botanist 12: 29-47. https://doi.org/10.3897/italianbotanist.12.70675
Figure 4 LM images of the cross-sections of P. malgassicum leaf (A–F) and stem (G, H) APAS positivity of trichomes and epidermis cells BPAS positivity of the epidermal cells and terpenic droplet below the first layer of cells C staining with FeCl3 D leaf lamina positive to Wagner staining E detail of D showing alkaloid droplets in the hypodermis cell F detail of D showing alkaloid droplets in hypodermis idioblasts G stem stained with Toluidine blue showing the thick layer of collenchyma beneath the epidermis and the two concentric circles of vascular bundles H detail of G showing the wall thickenings of the angular collenchyma. Ad: Alkaloid droplet; Cb: cortical circle of vascular bundles; Co: angular collenchyma; cx: cortex; ep: epidermis; hp: hypodermis; Mb: medullary circle of vascular bundles; Pi: pith; Td: terpenic droplet; Tr: trichome; Uh: upper hypodermis.
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