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FIGURE 1 in Leech Collections from Washington State, with the Description of Two New Species of Placobdella (Annelida: Glossiphoniidae)

FIGURE 1. Map of Washington showing the 13 collection sites in solid circles. Grey areas indicate bodies of water.

FIGURES 2–6. Placobdella kwetlumye, n in Leech Collections from Washington State, with the Description of Two New Species of Placobdella (Annelida: Glossiphoniidae)

FIGURES 2–6. Placobdella kwetlumye, n. sp. 2. Paratype, dorsal view. 3. Paratype, ventral view. 4. Eggs attached to the venter, ventral view. 5. Illustration of the proboscis (p), compact salivary glands (s), and mycetomes (m), in dorsal view. 6. Illustration of the male and female reproductive systems, dorsal view; testisacs not shown. Scale bars = 5 mm.

FIGURES 7–13. Placobdella sophieae, n in Leech Collections from Washington State, with the Description of Two New Species of Placobdella (Annelida: Glossiphoniidae)

FIGURES 7–13. Placobdella sophieae, n. sp. 7. Holotype, dorsal view. 8. Holotype, ventral view. 9. Eyespots showing characteristic "placobdellid" arrangement. 10. Posterior sucker showing the pedicel (peduncle). 11. Posterior sucker showing dorsal papilla. 12. Proboscis (p) and bundle of ductules (d), dorsal view; mycetomes not shown. Diffuse salivary glands indicated by arrows. 13. Dorsal view of the male and female reproductive systems. Scale bars = 3 mm.

FIGURES 14–19 in Leech Collections from Washington State, with the Description of Two New Species of Placobdella (Annelida: Glossiphoniidae)

FIGURES 14–19. Glossiphoniids found in Washington in this study; dorsal and ventral views, left and right respectively. 14. Glossiphonia elegans. 15. Helobdella elongata. 16. H. modesta. 17. Helobdella papillata. 18. Placobdella montifera. 19. Theromyzon cf. rude. Scale bars = 5 mm.

FIGURE 7 in Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada

FIGURE 7. Maximum likelihood tree based on the newly derived and GenBank COI sequences of Placobdella (lnL = - 7982.3955). All non-focal species-level taxa are collapsed when possible, with lone or non-collapsible tips showing GenBank accession numbers. Placobdella akahkway n. sp. is indicated within the green box, with new sequences indicated in bold. Maximum likelihood bootstrap support values are shown above each node and parsimony bootstrap support values below each node. 100% support values are indicated by squares for maximum likelihood and circles for parsimony. Asterisks indicate nodes not represented in the maximum parsimony tree.

FIGURE 6. Placobdella akahkway, n in Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada

FIGURE 6. Placobdella akahkway, n. sp. reproductive systems showing the atrial cornuae (ac) and ejaculatory ducts (ed) of the male reproductive system, as well as the ovaries (ov) of the female reproductive system.

FIGURE 5. Placobdella akahkway, n in Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada

FIGURE 5. Placobdella akahkway, n. sp. internal features showing the proboscis (pr), compact salivary glands (sg), bacteriomes (bac) and the esophagus (es). Arrowheads point to some of the diffuse salivary tissues, which are outlined in the schematic drawing and discussed further in the text.

FIGURE 3. Placobdella akahkway, n in Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada

FIGURE 3. Placobdella akahkway, n. sp. Diagram showing segmentation and gross papillation pattern of the holotype specimen. Detailed papillation pattern from somites XVI–XVIII shown as an inset to the right.

FIGURE 4. Placobdella akahkway, n in Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada

FIGURE 4. Placobdella akahkway, n. sp. external features. (A) eyespots (ey); (B) anus (an) and pre-anal papillae; (C) mouth pore (mp); (D) male (mg) and female (fg) gonopores.

FIGURE 2. Placobdella akahkway, n in Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada

FIGURE 2. Placobdella akahkway, n. sp. dorsal (left) and ventral (right) views. (A) Holotype, ROMIZI11495; (B) Paratype, ROMIZI11464, with young attached to the venter; (C) Paratype, ROMIZI11494; (D) Paratype, ROMIZI11542; (E) Paratype, ROMIZI11247.

FIGURE 1 in Placobdella akahkway n. sp. (Clitellata: Hirudinea: Glossiphoniidae), a new leech species from central Canada

FIGURE 1. Map of Canada showing the collection sites for specimens of Placobdella akahkway n. sp. in solid green circles. The star indicates the type locality.

FIGURE 14 in Description and taxonomic analysis of a tuberculated turtle leech from the Outer Banks, North Carolina, USA, provisionally identified as Placobdella multilineata Moore, 1953 (Hirudinea: Glossiphoniidae)

FIGURE 14. First feeding in the Albemarle turtle leech. Shown are three pre-juvenile individuals collected soon after taking their first blood meal on a turtle host. Their teguments are still transparent and the body shape is cylindroid. A, 5.0 mm (PM-11a) on Chelydra serpentina. B, 8.3 mm (PM-26) on Chrysemys floridana. C, 7.8 mm (PM-24a) on Kinosternum odoratus. Specimen A was collected within hours of first feeding and already had diluted blood in its crop. The intermediate rows of tubercles on the a2 annulus were discernible but do not show up in this image. In specimen B the tip of its lengthy proboscis protrudes from the apical mouth. The darkened rectum indicates some digestion of blood had already occurred. This correlates with the faintly pigmented tegument of this specimen. In specimen C the crooked proboscis and compact salivary glands are visibly well developed at this stage. Scale bars: 0.5 mm.

FIGURE 10 in Description and taxonomic analysis of a tuberculated turtle leech from the Outer Banks, North Carolina, USA, provisionally identified as Placobdella multilineata Moore, 1953 (Hirudinea: Glossiphoniidae)

FIGURE 10. Accessory 'eyes' as shown here are strongly expressed in about one-third of the Albemarle turtle leeches. Shown are close-ups of the head regions of two individuals. A, C, dorsal and ventral views, respectively, of specimen (PM-15a). B, dorsal view of extended specimen (PM-14b). Such heavily pigmented individuals typically display paramedial stripes on dorsum (pd) and on venter (vs), respectively. Scale bar: 1.0 mm.

FIGURE 12 in Description and taxonomic analysis of a tuberculated turtle leech from the Outer Banks, North Carolina, USA, provisionally identified as Placobdella multilineata Moore, 1953 (Hirudinea: Glossiphoniidae)

FIGURE 12. This scheme summarises appearance of key tubercles during development of the Albemarle turtle leech. Depicted are dorsal views of a mid-body segment, comprised of three annuli a1, a2 and a3, respectively. A, pre-feeding embryo (5–7 mm). B, post-feeding juvenile (10–15 mm). C, heavily tuberculated adult (20–40 mm). The intermediate tubercles (ti) arise first on the a2 annulus, and remain most prominent throughout life. Soon thereafter, tubercles arise in medial position (tm) on the a2 annulus, and in paramedial position (tp) on the a3 annulus. The indicated tubercles constitute a flattened 'W' configuration on each segment (red dashes), a taxonomically useful character specific to this species at virtually all stages of its life cycle. Together these respective tubercles constitute five longitudinal rows on the dorsum (see also Fig. 15A, B).

FIGURE 13 in Description and taxonomic analysis of a tuberculated turtle leech from the Outer Banks, North Carolina, USA, provisionally identified as Placobdella multilineata Moore, 1953 (Hirudinea: Glossiphoniidae)

FIGURE 13. Early development of the Albemarle turtle leech. Shown are unfed, unpigmented embryos of varying ages depicting progressive changes in foregut and crop caeca. A, 4.4 mm (PM-15e), ventral view. B, 4.8 mm (PM-6b), dorsal view. C, 6.0 mm (PM-15h), ventral view. In specimen A the cuticle (cu) separated from the anterior part of the body during preservation. The proboscis (p) is already relatively long and penetrates through the brain (b). The relative length of the proboscis is corroborated by the long cuticle lining the proboscis sheath (cup) emerging from the mouth. Scale bars: 0.5 mm.

FIGURE 7 in Description and taxonomic analysis of a tuberculated turtle leech from the Outer Banks, North Carolina, USA, provisionally identified as Placobdella multilineata Moore, 1953 (Hirudinea: Glossiphoniidae)

FIGURE 7. Variation of the dorsal stripe and tubercles in the Albemarle turtle leech. A, interrupted dorsal stripe (PM-15a). B, continuous dorsal stripe (PM-15c). C, no discernible dorsal stripe (PM-12). The primary tubercles, ti and tm on the a2 annulus, and tp on the a3 annulus, together with their bilateral counterparts, constitute a flattened letter 'W'. This configuration is specific for the Albemarle turtle leech. Furthermore, in this species the intermediate tubercles (ti) are invariably the most prominent. Dorsal views. A, B, LED light; C, incandescent light. Scale bar: 10 mm.