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Figure 3. SDS PAGE and Western blots with rEnSerp1 in Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

Figure 3. SDS PAGE and Western blots with rEnSerp1, ESP and CWE. Lines 1–3, 1D gel. Lines 4–10, Western blot. M, protein standard; 1, rEnSerp1; 2, ESP; 3, CWE sample; 4, rEnSerp1 with anti-HIS antibodies; 5, rEnSerp1 without primary antibodies; 6, rEnSerp1 with anti-rEnSerp1 sera; 7, rEnSerp1 with pre-immune sera; 8, ESP with anti-rEnSerp1 sera; 9, ESP with pre-immune sera; 10, CWE with anti-rEnSerp1 sera. Arrow points to the expected recombinant EnSerp1 band. Arrowhead points to the natural form of EnSerp1 in the ESP sample. Dots indicate fragmented parts of rEnSerp1.

Figure 1 in Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

Figure 1. (A) Alignment of the EnSerp1 sequence with four of the most similar serpin sequences of other platyhelminths: 1, EnSerp1 from Eudiplozoon nipponicum (GenBank: MF288891.1); 2, Echinococcus multilocularis (GenBank: CDS35969.1); 3, Schistosoma haematobium (GenBank: XP_012797533.1); 4, Echinococcus granulosus (GenBank: CDS22753.1); 5, Taenia solium (GenBank: ATG83400.1). Conserved motifs characteristic for serpins are highlighted. The serpin motif (E342 – E346) shown in red is part of the reactive centre loop (RCL, A347 – N365), shown in green. Immediately after RCL, follows serpin signature (F366 – I376) in yellow. Scissile bond is situated within the RCL between P1 (F358) and P1' (C359) residue, shown in magenta. (B) Predicted 3D structure of EnSerp1. Coloured areas of the molecule correspond to the sequence highlighted in Figure 1A. (C) RCL and β-sheet A. After the peptidase cleaves the scissile bond within RCL (in orange), the residual part of RCL is incorporated as a new strand into β-sheet A (in cyan).

Figure 4 in Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

Figure 4. The inhibitory effect of rEnSerp1 on selected SPs. (A) trypsin; (B) factor Xa; (C) plasmin; (D) plasma kallikrein. Results of assays with a bacterial lysate instead of rEnSerp1: (E) factor Xa and F, trypsin. Data are expressed as a mean value ± standard deviation.

Figure 2. A in Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

Figure 2. A phylogram of platyhelminth serpin homologs, Bayesian inference analysis. Values along the branches indicate posterior probabilities and bootstrap values resulting from Bayesian inference and Maximum likelihood analyses, respectively. Proportional lengths of the branches correspond to the expected number of amino acid substitutions per site. The resulting tree is mid-point rooted in order to visualise the clustering of representative subfamilies. Newly obtained Eudiplozoon nipponicum serpin homolog (EnSerp1) is labelled red.

Fig. 11. Plectanocotyle jeanloujustinei n in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 11. Plectanocotyle jeanloujustinei n. sp. ex Chelidonichthys lastoviza from the western Mediterranean. Detail of the reproductive organs in the region of ovary, ventral view (HEL 1715).

Fig. 10. Plectanocotyle jeanloujustinei n in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 10. Plectanocotyle jeanloujustinei n. sp. ex Chelidonichthys lastoviza from the western Mediterranean. Disposition of clamps sclerites. A, Dorsal jaw. B, Ventral jaw. C, Clamp, dorsal view (HEL 1713).

Fig. 9. Plectanocotyle jeanloujustinei n in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 9. Plectanocotyle jeanloujustinei n. sp. ex Chelidonichthys lastoviza from the western Mediterranean. A, Body, holotype, ventral view (HEL 1721). B, Clamp, hologenophore, ventral view (HEL 1731). C, Clamp, paratype, dorsal view (HEL 1717). D, terminal lappet, paratype (HEL 1717). E, Anterior end showing male copulatory organ, paratype, ventral view (HEL 1720). F, Egg, paratype (HEL 1714).

Fig. 4 in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 4. Tree inferred using the ML method based on the cox1 sequence data; only bootstrap values higher than 70 are indicated. The newly generated sequences of Plectanocotyle gurnardi sensu stricto are indicated in blue. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 8 in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 8. Plectanocotyle gurnardi (Van Beneden and Hesse, 1863) sensu stricto ex Eutrigla gurnardus from the North Sea, Sweden, Detail of the reproductive organs in the region of ovary, ventral view (SMNH 216584).

Fig. 5 in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 5. Tree inferred using the ML method based on the 28S rDNA sequence data; only bootstrap values higher than 70 are indicated. The newly generated sequences are indicated in bold. All sequences of Plectanocotyle gurnardi from the type-host Eutrigla gurnardus clustered in a single clade. * The polyopisthocotylean parasite annotated on GenBank as "Plectanocotyloides obscurum"; the host as "Aspitrigla obscura".

Fig. 3. Plectanocotyle jeanloujustinei n in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 3. Plectanocotyle jeanloujustinei n. sp. ex Chelidonichthys lastoviza from the western Mediterranean (Algeria), hologenophores. A-E, Body lacking only the haptor. F. Body lacking a lateral part posterior part. G-H, anterior end of the body including the male copulatory organ. The missing parts were excised and used for DNA extraction. A, (HEL1729). B, (HEL1730). C, (HEL1732). D, (HEL1733). E, (HEL1734). F, (HEL1731). G, (HEL1727). H, (HEL17287).

Fig. 2 in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 2. Plectanocotyle gurnardi (Van Beneden and Hesse, 1863) sensu stricto ex Eutrigla gurnardus from the North Sea, Sweden, hologenophores, body lacking only a lateral part; excised used for DNA extraction A, (SMNH 216644). B, (SMNH 216645). C, (SMNH 216646).

Fig. 1 in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 1. One of the unstudied specimens of Plectanocotyle gurnardi (Van Beneden and Hesse, 1863) from the type-host Eutrigla gurnardus available for study before this paper, and that hinted the presence of Plectanocotyle gurnardi in Swedish waters of the North Sea. The slide containing two specimens of Plectanocotyle gurnardi labeled as "Phyllocotyle gurnardi": unstudied specimens collected by Theodor Odhner from the gills of Eutrigla gurnardus from Kristineberg, Sweden, Northeast Atlantic.

Fig. 6 in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 6. Plectanocotyle gurnardi (Van Beneden and Hesse, 1863) sensu stricto ex Eutrigla gurnardus from the North Sea, Sweden. A, Body, ventral view (SMNH 216639). B, Clamp, ventral view (SMNH 216593). C, Anterior end showing male copulatory organ, ventral view (SMNH 216586). D, Egg, (SMNH 216594).

Fig. 7 in "Something old, something new, something borrowed, and the oioxeny is true": description of Plectanocotyle jeanloujustinei n. sp. (Polyopisthocotylea, Plectanocotylidae) from the MNHN Helminthology collection with novel molecular and morphological data for P. gurnardi (Van Beneden & Hesse, 1863) (sensu stricto) from Sweden

Fig. 7. Plectanocotyle gurnardi (Van Beneden and Hesse, 1863) sensu stricto ex Eutrigla gurnardus from the North Sea, Sweden, disposition of clamps sclerites. A, Dorsal jaw. B, Ventral jaw. C, Clamp, ventral view (SMNH 216593). 3.2. Morphology