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Fig. 1 in Molecular evidence of hybridisation in two invasive species of Pomacea (Gastropoda: Ampullariidae) in Peninsular Malaysia

Fig. 1. Geographical location and habitat type of sampling sites of Pomacea species in Peninsular Malaysia.

Fig. 4 in Molecular evidence of hybridisation in two invasive species of Pomacea (Gastropoda: Ampullariidae) in Peninsular Malaysia

Fig. 4. Median-joining haplotype network of Pomacea canaliculata and P. maculata sequences from Peninsular Malaysia (N=108 from 90 individuals) and the native ranges (N=105) based on 409 nucleotides of the EF1α gene. The network shows the relationship between haplotypes from different geographic regions based on sequence similarity. Unique sequences within each individual were included in the alignment (sequences for homozygotes were not doubled). Node colours represent the (A) geographic location and (B) species identity of the sequences (see legends). Each node represents a unique haplotype and node size is proportional to the haplotype frequency. Branches between nodes indicate a single nucleotide substitution unless denoted by numerical values for multiple nucleotide substitutions. Red (A) and black (B) nodes represent hypothetical ancestors or unsampled haplotypes. Two major groups are framed in grey dotted lines; P. canaliculata and P. maculata.

Fig. 3 in Molecular evidence of hybridisation in two invasive species of Pomacea (Gastropoda: Ampullariidae) in Peninsular Malaysia

Fig. 3. Bayesian inference phylograms depicting relationship of P. canaliculata and P. maculata from Peninsular Malaysia and Pomacea spp. from other regions based on the (A) mitochondrial COI and (B) nuclear EF1α markers. Kuantan, Tasik ChinChin, Limbat Lembu, Subang Jaya, Putrajaya, Guar Cempedak, Pasir Gudang, Sekinchan, and Temoh refer to geographic locations in Peninsular Malaysia where specimens in this study were collected. Bayesian posterior probabilities/maximum likelihood bootstrap supports are indicated by nodal values. Pomacea difussa and P. scalaris were used to root the phylogenies. Pomacea canaliculata and P. maculata clades are highlighted in green and blue, respectively. Underlined taxa marked with '*' indicate interspecific heterozygous individuals whereas taxa in red and marked with '**' are COI-EF1α mito-nuclear incongruences.

Fig. 2 in Molecular evidence of hybridisation in two invasive species of Pomacea (Gastropoda: Ampullariidae) in Peninsular Malaysia

Fig. 2. Representative agarose gel electrophoresis image showing the ApaLI-digested EF1α amplicons for 14 specimens from Putrajaya. The single band, two-band, and three-band RFLP profiles indicate Pomacea canaliculata, P. maculata, and interspecific heterozygous hybrids, respectively.

Figure 4 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figure 4. Box plot of the snail's activity time (min) for all the activity and behaviour experiment, represented according to the sex of the experimental snail, the absence or presence of a partner and its sex.

Figure 1 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figure 1. Arrangement of aquaria used to assess the activity and behaviour of snails in the presence of a congener of the same or different sex. R: clock; T: thermometer.

Figure 3 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figure 3. Survivorship as a function of time (days) and treatment. The intersection with the horizontal line indicates the median of the survival time for each treatment. The rectangle (▯) that encloses the sex indicates the individual considered in the analysis in the treatments with both sexes. The crosses (x) indicate that the treatments were censored.

Figura 1 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figura 1. Arreglo de acuarios utilizado para evaluar la actividad y el comportamiento de los caracoles en función de la presencia de un congénere del mismo o de distinto sexo. R: reloj; T: termómetro.

Figura 2. A in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figura 2. A) Superposición digital de 200 imágenes tomadas durante el tercer cuarto del experimento de actividad y comportamiento utilizada para calcular el porcentaje de área explorada. R: reloj; T: termómetro. B) Simulación realizada para reconstruir el área explorada por el macho y la hembra por separado en el tratamiento (Ÿÿ) que incluía un caracol de cada sexo. Figure 2. A) Digital overlap of 200 images taken during the third quarter of the experiment of activity and behaviour used to calculate the percentage of explored area. R: clock; T: thermometer. B) Simulation carried out to reconstruct the area explored by the male and the female separately in the treatment (Ÿÿ) that included one snail of each sex.

Figura 4 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figura 4. Diagrama de cajas y brazos del tiempo de actividad (min) de los caracoles para el experimento completo de actividad y comportamiento, representados en función del sexo del caracol experimental y de la ausencia o presencia de una pareja y del sexo de la misma.

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Fig. 4 in Fig. 3 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 4. Probability of sexual differentiation related to total length of A. marmorata collected from three rivers on Amami-Oshima Island, Japan. The black lines and shaded areas indicate the regression lines and 95% confidential intervals, respectively.

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Fig. 1 in Fig. 3 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 1. Maps of the study area on Amami-Oshima Island, Kagoshima Prefecture, Japan. (a) Location of the Amami-Oshima Island in Japan. (b) Location of the study area on Amami-Oshima Island. (c) Map of the study area. Open circles indicate sampling sites for Anguilla marmorata.

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Fig. 3 in Fig. 3 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 3. Difference between Amami-Oshima Island and Sulawesi Island (Hagihara et al. 2018a) in growth rate and sex ratio of A. marmorata. A, Mean growth rate and SD of female, male, and UD in Amami-Oshima Island and Sulawesi Island. B, Sex ratio of sexually differentiated individuals in Amami-Oshima Island and Sulawesi Island.

Fig. 6 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 6. Canonical Correlation Analysis (CCA) of rotifer assamblages with environmental variables. Relationship between species and environmental variables (Left). Relationship between sampling site and environmental variables (Right). Abbreviations used in the figures: H, Han River downstream (blue circle); R, reservoir (green circle); T, tidal creek; Bra_cal, Brachionus calyciflorus; Bra_urc, B. urceolaris; Bra_ang, B. angularis; Bra_pli, B. plicatilis; Bra_fal, B. falcatus; Bra_for, B. forficula; Anu_fis, Anuraeopsis fissa; Pol_vul, Polyarthra vulgaris; Pol_eur, P. euryptera; Fil_nov, Filinia novaezealandiae; Fil_lon, F. longiseta; Ker_coc, Keratella cochlearis; Ker_tec, K. tecta; Ker_tro, K. tropica; Lec_clo, Lecane closterocerca; Lec_sti, L. stichaea; Lec_ste, L. stenroosi; Lec_ham, L. hamata; Lec_lun, L. Luna; Hex_mir, Hexarthra mira; Tri_pus, Trichocerca pusilla; Tri_sim, T. similis; Tri_sty, T. stylata; Tri_cyl, T. cylindrica; Tri_cap, T. capucina; Asc_ova, Ascomorpha ovalis; Syn_sty, Synchaeta stylata; Syn_tre, S. Tremula; Syn_obl, S. oblonga; Col_adr, Colurella adriatica; Plo_tru, Ploesoma truncatum.

Fig. 5 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 5. Rotifer community indices among three water bodies. (A) Rotifer total abundance; (B) Ejsmont-Karabin's rotifer trophic state index; (C) Sladecek's B/T quotient and Keratella-index (KIN) values; (D) α biodiversity indices. Letters indicate sample means that are similar (same letter) or significantly different (different letter) among different water bodies; blank represents no significant difference. Abbreviations used in the figures: HD, Han River downstream; RE, reservoir; TC, tidal creek.

Fig. 4 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 4. The non-metric multidimensional scaling (NMDS) plots of rotifer community in Shantou. Abbreviations used in the figures: HD, Han River downstream; RE, reservoir; TC, tidal creek.

Fig. 3 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 3. The proportions (Y axis on left) and the abundances (Y axis on right) of the dominant rotifers in different sampling sites in Shantou. The lines represent the total abundances and the column represent the rotifer composition. Abbreviations used in the figures: HD, Han River downstream; RE, reservoir; TC, tidal creek.

Fig. 2 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 2. Environmental factors and trophic states in the different water bodies. Letters indicate sample means that are similar (same letter) or significantly different (different letter) among different water bodies; blank represents no significant difference. Abbreviations used in the figures: HD, Han River downstream; RE, reservoir; TC, tidal creek.

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Fig. 1 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 1. Map showing the Ryukyu Archipelago and the Pacific coast of Japan with information on the Kuroshio Current (KC) in the northwestern Pacific region (a) and Google Earth photograph showing the southern Boso Peninsula, Japan (b). The blue line shows the KC. The dotted line indicates the Ryukyu Archipelago (RK). Other locations referred to in the main document are as follows: IG, Ishigakijima Island; ON, Okinawajima Island; TK, Takarajima Island; HJ, Hachijojima Island; OT, Otsuki; SH, Shirahama; BP, Boso Peninsula (a); BN, Banda; OK, Okinoshima, NJ, Cape Nojimazaki; SR, Shirosaki; KT, Katsuura; TB, Tateyama Bay (b).

Fig. 1 in Pomacea occulta Salazar-Vallejo 2023, nov. sp.

Fig. 1. Location of the study area and the sampling sites covered in Shantou City. Abbreviations used in the figures: HD, Han River downstream; RE, reservoir; TC, tidal creek.