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FIGURE 6 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 6. Right valve internal views of Popenaias berezai n. sp. Localities correspond to those of Fig. 5. (A) Holotype (CNMO 8037, ex. INHS 90397.5); (B) Paratype (INHS 90397.1); (C) Paratype (INHS 90183.3); (D) Paratype (INHS 90183.1); (E) Paratype (INHS 90175.3); (F) Paratype (INHS 90175.2). Scale bar 1 cm.

FIGURE 3 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 3. Maximum parsimony cladogram for the concatenated mitochondrial and nuclear DNA (cox1, 16S, and ANT) sequence dataset. Bootstrap support values are shown in percent along the branches. The tree is rooted with Quadrula quadrula. Major clades are annotated. Colored box along clades corresponds to the target taxa: blue, Popenaias popeii; green, P. ganina; orange, P. berezai n. sp.

FIGURE 1 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 1. Collection localities for Popenaias species for molecular and morphometric analyses used in this study. Colored symbols represent target species (blue circles, Popenaias popeii; green squares, P. ganina; orange diamonds, P. berezai n. sp.). Shading indicates elevation where darker colors represent higher altitude.

FIGURE 5 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 5. Right valve external views of Popenaias berezai n. sp. (A–F). (A) Holotype (CNMO 8037, ex. INHS 90397.5), 73 mm length, from the Río Valles, Estación Micos, 20 km NW of Ciudad Valles, San Luis Potosí, Mexico; (B) Paratype (INHS 90397.1), 62 mm, from the locality same as A; (C) Paratype (INHS 90183.3), 59 mm, from the Río Tampaón (Río Pánuco) Álvaro Obregón (Pujal), at confluence of the ríos Valles and Tampaón; (D) Paratype (INHS 90183.1), 67 mm length, from the locality same as C; (E) Paratype (INHS 90175.3), 53 mm, from the Río Tancuilin, 0.5 km south of Jalpilla, San Luis Potosí, Mexico; (F) Paratype (INHS 90175.2), 50 mm, from the locality same as E. White bar denotes 1 cm scale.

FIGURE 9 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 9. Presumptive historic and current distributional ranges of (A) Popenaias berezai n. sp. in Mexican Gulf coastal rivers, (B) Popenaias ganina in the Río Gallinas of the Río Pánuco basin, (C) Popenaias popeii in the Rio Grande basin of Mexico and the United States, and (D) Popenaias tehuantepecensis including Nephronaias rowellii (Lea, 1859) in Central America.

FIGURE 8 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 8. Right valve external shells. (A) Popenaias berezai n. sp., Río Valles, San Luis Potosí, Mexico, Holotype CNMO 8037, ex. INHS 90397.5; (B) Popenaias ganina, Río Gallinas, San Luis Potosí, Mexico, Lectotype ANSP 99523a; (C) Popenaias popeii, Río Salado, Nuevo León, Mexico, Lectotype USNM 85895.

FIGURE 7 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 7. Soft anatomy of Popenaias berezai n. sp. Localities correspond to those of Fig. 5. (A) Holotype, non-gravid (CNMO 8037, ex. INHS 90397.5); (B) Paratype, gravid individual (INHS 90397.1); (C) Paratype, non-gravid (INHS 90183.3); (D) gravid individual (INHS 90183.1); (E) Paratype, non-gravid (INHS 90175.3); (F) Paratype, gravid individual (INHS 90175.2). Scale bar 1 cm.

FIGURE 4 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 4. Biplots from principal component analysis (PCA; A) and canonical variate analysis (CVA; B) of Fourier shape morphometrics. Colors and shapes of points correspond to species: blue circles, Popenaias popeii; green squares, P. ganina; orange diamonds, P. berezai n. sp. Polygons enclose convex hulls of each species. Outlined shell shapes represent a mean shape (top-right) and ±2 × SD on PC1 and PC2 axes.

FIGURE 2 in A new species of freshwater mussel in the genus Popenaias Frierson, 1927, from the Gulf coastal rivers of central Mexico (Bivalvia: Unionida: Unionidae) with comments on the genus

FIGURE 2. Phylogenetic tree reconstructed by Bayesian inference (BI) for the concatenated mitochondrial and nuclear DNA (cox1, 16S, and ANT) sequence dataset. Nodal supports (i.e., posterior probability values from BI analysis and ultrafast bootstrap support values from maximum likelihood analysis) are shown as percentages along the branches. The tree is rooted with Quadrula quadrula. Major clades are annotated. Colored box along clades corresponds to the target taxa (blue, Popenaias popeii; green, P. ganina; orange, P. berezai n. sp.).