Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
24
datasets available to search
ShareScore release 0.9.0
Dataset results
24 results for “Post-transcriptional gene regulation”
Direct molecular evidence for an ancient, conserved developmental toolkit controlling post-transcriptional gene regulation in land plants
<p>In plants, miRNA production is orchestrated by a suite of proteins that control transcription of the pri-miRNA gene, post-transcriptional processing and nuclear export of the mature miRNA. Post-transcriptional processing of miRNAs is controlled by a pair of physically-interacting proteins, HYL1 and DCL1. However, the evolutionary history and structural basis of the HYL1-DCL1 interaction is unknown. Here we use ancestral sequence reconstruction and functional characterization of ancestral HYL1 <em>in vitro</em> and in <em>Arabidopsis thaliana </em>to better understand the origin and evolution of the HYL1-DCL1 interaction and its impact on miRNA production and plant development. We found the ancestral plant HYL1 evolved high affinity for both double-stranded RNA (dsRNA) and its DCL1 partner before the divergence of mosses from seed plants (~500 Ma), and these high-affinity interactions remained largely conserved throughout plant evolutionary history. Structural modeling and molecular binding experiments suggest that the second of two double-stranded RNA-binding motifs (DSRMs) in HYL1 may interact tightly with the first of two C-terminal DCL1 DSRMs to mediate the HYL1-DCL1 physical interaction necessary for efficient miRNA production. Transgenic expression of the nearly 200 Ma-old ancestral flowering-plant HYL1 in <em>A. thaliana</em> was sufficient to rescue many key aspects of plant development disrupted by HYL1<sup>-</sup> knockout and restored near-native miRNA production, suggesting that the functional partnership of HYL1-DCL1 originated very early in and was strongly conserved throughout the evolutionary history of terrestrial plants. Overall, our results are consistent with a model in which miRNA-based gene regulation evolved as part of a conserved plant ‘developmental toolkit’.</p>
Data from: Quantitative proteomics reveals key roles for post-transcriptional gene regulation in the molecular pathology of FSHD
DUX4 is a transcription factor whose misexpression in skeletal muscle causes facioscapulohumeral muscular dystrophy (FSHD). While DUX4's transcriptional activity has been extensively characterized, the DUX4-induced proteome remains undescribed. Here, we report concurrent measurement of RNA and protein levels in DUX4-expressing cells via RNA-seq and quantitative mass spectrometry. DUX4 transcriptional targets were robustly translated, confirming the likely clinical relevance of proposed FSHD biomarkers. However, a multitude of mRNAs and proteins exhibited discordant expression changes upon DUX4 expression. Our dataset revealed unexpected proteomic, but not transcriptomic, dysregulation of diverse molecular pathways, including Golgi apparatus fragmentation, as well as extensive post-transcriptional buffering of stress response genes. Key components of RNA degradation machineries, including UPF1, UPF3B, and XRN1, exhibited suppressed protein, but not mRNA, levels, explaining the build-up of aberrant RNAs that characterizes DUX4-expressing cells. Our results provide a resource for the FSHD community and illustrate the importance of post-transcriptional processes to DUX4-induced pathology.
Data from: Quantitative proteomics reveals key roles for post-transcriptional gene regulation in the molecular pathology of FSHD
Open the record for dataset details and reuse information.
TRAP-Seq uncovers post-transcriptional gene regulation after acute sleep deprivation
GEO Series GSE156925. Mus musculus. 11 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome and proteome quantification of a tumor model provides novel insights into post-transcriptional gene regulation
GEO Series GSE51412. Drosophila melanogaster. 6 samples. Type: Expression profiling by high throughput sequencing.
A role for ZNF598 in post-transcriptional gene regulation
GEO Series GSE116126. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
Genome-wide mapping of the HY5-mediated gene networks in Arabidopsis that involve both transcriptional and post-transcriptional regulations: ChIP-Chip
GEO Series GSE24973. Arabidopsis thaliana. 12 samples. Type: Genome binding/occupancy profiling by genome tiling array.
Low RNA stability signifies increased post-transcriptional regulation of cell identity genes
GEO Series GSE185990. Homo sapiens. 14 samples. Type: Expression profiling by high throughput sequencing.
PTBP1 controls intestinal epithelial regeneration through post-transcriptional regulation of gene expression
GEO Series GSE185499. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
Post-transcriptionally regulated genes are essential for pluripotent stem cell survival
GEO Series GSE184546. Homo sapiens. 15 samples. Type: Expression profiling by array.
Genome-wide mapping of the HY5-mediated gene networks in Arabidopsis that involve both transcriptional and post-transcriptional regulations
GEO Series GSE24974. Arabidopsis thaliana. 14 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by genome tiling array.
Genome-wide mapping of the HY5-mediated gene networks in Arabidopsis that involve both transcriptional and post-transcriptional regulations: RNA-Seq
GEO Series GSE24970. Arabidopsis thaliana. 2 samples. Type: Expression profiling by high throughput sequencing.
Post-transcriptional gene regulation by microRNA-194 promotes neuroendocrine transdifferentiation in prostate cancer [ChIP-Seq]
GEO Series GSE162319. Homo sapiens. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Post-transcriptional gene regulation by microRNA-194 promotes neuroendocrine transdifferentiation in prostate cancer
GEO Series GSE137071. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Post-transcriptionally regulated genes are essential for pluripotent stem cell survival
GEO Series GSE199820. Homo sapiens. 18 samples. Type: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing.
Genome-wide analysis of genes regulated transcriptionally and post-transcriptionally by HTLV-I p30
GEO Series GSE16098. Homo sapiens. 4 samples. Type: Expression profiling by array.
Oo-site: A dashboard to visualize gene expression during Drosophila oogenesis reveals meiotic entry is regulated post-transcriptionally
GEO Series GSE195893. Drosophila melanogaster. 4 samples. Type: Expression profiling by high throughput sequencing.
POST-TRANSCRIPTIONAL REGULATION OF KRAS GENE: MECHANISM INVOLVING 5’UTR RNA G-QUADRUPLEXES, LONG NONCODING RNA AND NUCLEAR RIBONUCLEOPROTEIN A1
GEO Series GSE283011. Homo sapiens. 8 samples. Type: Other.
Post-transcriptional gene regulation by microRNA-194 promotes neuroendocrine transdifferentiation in prostate cancer
GEO Series GSE137070. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
Post-transcriptional regulation of necroptosis gene EIF2AK2 in peripheral blood of bronchopulmonary dysplasia
GEO Series GSE219156. Homo sapiens. 18 samples. Type: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.