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zenodo40/100

Fig. 6 in An overview of the Dactylosomatidae (Apicomplexa: Adeleorina: Dactylosomatidae), with the description of Dactylosoma kermiti n. sp. parasitising Ptychadena anchietae and Sclerophrys gutturalis from South Africa

Fig. 6. (A–K). Possible development of Dactylosoma kermiti n. sp. in the gut or haemocoel from the mosquitoes Uranotaenia (Pseudoficalbia) mashonaensis and U. (Pfc.) montana, from infected Sclerophrys gutturalis. (A) Intracellular meront. (B) Intra- and extracellular meront. (C–D) Merging of gametes. (E) Ookinete. (F) Immature oocyst. (G–I) Free sporozoites. (J) Probable meront producing immature merozoites. (K) Probable meront, producing long and slender mature merozoites. Vacuoles – arrow (A–B); Nucleus – arrow (D–K); Condensed chromatin – arrowhead (B, D–K). Scale bars 10 μm.

opencc-by-4.0Apr 2020View details →
zenodo40/100

Fig. 5 in An overview of the Dactylosomatidae (Apicomplexa: Adeleorina: Dactylosomatidae), with the description of Dactylosoma kermiti n. sp. parasitising Ptychadena anchietae and Sclerophrys gutturalis from South Africa

Fig. 5. (A-D). dipterans observed feeding on Ptychadena anchietae and Sclerophrys gutturalis in situ.(A–B). African phlebotomine sand flies (arrows) Sergentomyia sp. feeding on Ptychadena anchietae in situ. (C–D) Mosquitoes (arrows), Uranotaenia (Pseudoficalbia) mashonaensis and U. (Pfc.) montana feeding on Sclerophrys gutturalis in situ.

opencc-by-4.0Apr 2020View details →
zenodo40/100

Fig. 4 in An overview of the Dactylosomatidae (Apicomplexa: Adeleorina: Dactylosomatidae), with the description of Dactylosoma kermiti n. sp. parasitising Ptychadena anchietae and Sclerophrys gutturalis from South Africa

Fig. 4. Consensus phylogram of haemogregarines based on 18S rDNA sequences. Tree topologies for Bayesian inference (BI) and Maximum likelihood (ML) analyses were similar (represented on the ML tree), showing the phylogenetic relationships for D. kermiti n. sp. and Dactylosoma sp. ex Pel. lessonae (represented in bold), compared to other species of Haemogregarina, Hepatozoon, Karyolysus, Hemolivia, and Adelina and Klossia as outgroup. Clades that neither produced 0.80 posterior probability (BI) or 70 bootstrap (ML) nodal support values were omitted. The scale bar represents 0.02 nucleotide substitutions per site. The host, geographical distribution (according to the zoogeographical realms), and if known the vector and life history cycle are also provided for the different sequences using symbols and pictograms. Asterisks (*) indicate the proposed life history strategy of D. kermiti n. sp. based on data from the current study.

opencc-by-4.0Apr 2020View details →
zenodo40/100

Fig. 1 in An overview of the Dactylosomatidae (Apicomplexa: Adeleorina: Dactylosomatidae), with the description of Dactylosoma kermiti n. sp. parasitising Ptychadena anchietae and Sclerophrys gutturalis from South Africa

Fig. 1. (A–L). Dactylosoma kermiti n. sp. from the grass frog Ptychadena anchietae. (A–H) Primary merogony. (A) Young trophozoite. (B–D) Trophozoites. (E) Young meront. (F–G) Primary meronts. (H) Merozoites. (I–L) Secondary merogony. (I) Secondary meront. (J) Immature gamont. (K) Gamont. (L) Extracellular gamont. Arrowheads show condensed chromatin (A–I); arrows show vacuoles (B–E). All images captured from the deposited slides [NMB P 534 – 535]. Scale bar 10 μm.

opencc-by-4.0Apr 2020View details →
zenodo40/100

Fig. 2 in An overview of the Dactylosomatidae (Apicomplexa: Adeleorina: Dactylosomatidae), with the description of Dactylosoma kermiti n. sp. parasitising Ptychadena anchietae and Sclerophrys gutturalis from South Africa

Fig. 2. (A–L). Dactylosoma kermiti n. sp. from the guttural toad Sclerophrys gutturalis. (A–D) Primary merogony. (A) Young trophozoite. (B) Trophozoites. (C) Young meront. (D–L) Secondary merogony. (D) Young secondary meront. (E) Secondary meront. (F–G) Secondary merozoites. (H–I) Gamont. (K) Extracellular gamont. (L) Secondary meront in leukocyte. Arrowheads show condensed chromatin (D–H, L); arrows show vacuoles (B) and merozoites (F–G). All images captured from the deposited slides [NMB P 536 – 537]. Scale bar 10 μm.

opencc-by-4.0Apr 2020View details →
zenodo40/100

Fig. 3 in An overview of the Dactylosomatidae (Apicomplexa: Adeleorina: Dactylosomatidae), with the description of Dactylosoma kermiti n. sp. parasitising Ptychadena anchietae and Sclerophrys gutturalis from South Africa

Fig. 3. (A–L). Dactylosoma sp. from Pelophylax lessonae. (A–D) Primary merogony. (A) Trophozoite. (B) Young meront. (C–G) Secondary meronts. (F–H) Merozoites, arrows. (I–L) Secondary merogony. (I) Young meront. (J) Meront. (K) Merozoite. (L) Gamont. Arrowheads show condensed chromatin; arrows show vacuoles (A) and merozoites (F–H). All images captured from the deposited slide [NMB P 538]. Scale bar 10 μm.

opencc-by-4.0Apr 2020View details →
zenodo40/100

FIG. 1 in Révision des espèces du groupe de Ptychadena stenocephala (Amphibia, Anura)

FIG. 1. — Localités-type des espèces du groupe de Ptychadena stenocephala; 1, Kassewe, Sierra Leone: Ptychadena arnei Perret, 1997; 2, Mont Nimba, Guinée: Ptychadena tournieri (Guibé & Lamotte, 1955) et Ptychadena pujoli Lamotte & Ohler, 1997; 3, Parc national de la Garamba, Zaïre: Ptychadena ingeri Perret, 1991; 4, Entebbe, Ouganda: Ptychadena stenocephala (Boulenger, 1901).

opencc-zeroDec 2000View details →
zenodo36/100

FIG. 2 in Révision des espèces du groupe de Ptychadena stenocephala (Amphibia, Anura)

FIG. 2. — Vue dorsale de Ptychadena tournieri, mâle adulte, MNHN 1995.1376. Échelle: 10 mm.

opencc-zeroDec 2000View details →
dryad32/100

Data from: The role of climatic and geological events in generating diversity in Ethiopian grass frogs (genus Ptychadena)

Ethiopia is a world biodiversity hotspot and harbours levels of biotic endemism unmatched in the Horn of Africa, largely due to topographic—and thus habitat—complexity, which results from a very active geological and climatic history. Among Ethiopian vertebrate fauna, amphibians harbour the highest levels of endemism, making amphibians a compelling system for the exploration of the impacts of Ethiopia's complex abiotic history on biotic diversification. Grass frogs of the genus Ptychadena are notably diverse in Ethiopia, where they have undergone an evolutionary radiation. We used molecular data and expanded taxon sampling to test for cryptic diversity and to explore diversification patterns in both the highland radiation and two widespread lowland Ptychadena. Species delimitation results support the presence of nine highland species and four lowland species in our dataset, and divergence dating suggests that both geologic events and climatic fluctuations played a complex and confounded role in the diversification of Ptychadena in Ethiopia. We rectify the taxonomy of the endemic P. neumanni species complex, elevating one formally synonymized name and describing three novel taxa. Finally, we describe two novel lowland Ptychadena species that occur in Ethiopia and may be more broadly distributed.

opencc-zeroDec 2016View details →
dryad32/100

Data from: Diversity of ridged frogs (Anura: Ptychadenidae: Ptychadena spp.) in wetlands of the upper Nile in Rwanda: morphological, bioacoustic, and molecular evidence

We investigated the distribution of species of the genus Ptychadena at nine sites at the upper Nile and its catchment in Rwanda. For species delimitation, we chose an integrative approach, combining morphological and bioacoustic data and DNA barcoding (mitochondrial 16S rRNA gene). We identified three species using independent evidence from the three different data sets: Ptychadena anchietae, Ptychadena porosissima, and a species of the Ptychadena mascareniensis group. The latter is undistinguishable genetically, bioacoustically, and morphologically from populations from Uganda, Kenya, and Egypt. We resurrect the name Ptychadena nilotica for these populations. The species differs strongly genetically from topotypic P. mascareniensis, and from clades referred to as P. cf. mascareniensis from Western and Central Africa. Morphologically, the three Rwandan species can be differentiated by their quantitative morphometrics (discriminant analysis, success rate: 98.3%) and by a number of qualitative characters of external morphology which are useful for identification in the field. The specific features of the advertisement call differ unequivocally among the three species and allow detection and identification in the field. We also provide quantitative descriptions of temporal and frequency structure of the release calls of two of the species and the distress calls of all three species. Finally, we compare the 16S sequences obtained from Rwandan specimens with those deposited in GenBank to estimate geographical distribution of taxa in Africa.

opencc-zeroDec 2012View details →
zenodo32/100

FIGURE 1 in The tadpole of Ptychadena schillukorum (Werner, 1908 "1907") (Amphibia: Anura: Ptychadenidae)

FIGURE 1. Tadpole of Ptychadena schillukorum from Pendjari Biosphere Reserve (Gosner stage 36) in dorsal and lateral view (scale bar 10 mm) and mouthpart (scale bar 1 mm); number of keratodonts sketched, no accurate count.

opennotspecifiedMay 2009View details →
dryad32/100

Data from: The role of climatic and geological events in generating diversity in Ethiopian grass frogs (genus Ptychadena)

Open the record for dataset details and reuse information.

publicJul 2017View details →
dryad32/100

Data from: Diversity of ridged frogs (Anura: Ptychadenidae: Ptychadena spp.) in wetlands of the upper Nile in Rwanda: morphological, bioacoustic, and molecular evidence

Open the record for dataset details and reuse information.

publicOct 2014View details →
zenodo28/100

Supplementary material 2 from: Reyes-Velasco J, Goutte S, Freilich X, Boissinot S (2021) Mitogenomics of historical type specimens clarifies the taxonomy of Ethiopian Ptychadena Boulenger, 1917 (Anura, Ptychadenidae). ZooKeys 1070: 135-149. https://doi.org/10.3897/zookeys.1070.66598

Table S1

opencc-zeroNov 2021View details →
zenodo28/100

Figure 1 from: Reyes-Velasco J, Goutte S, Freilich X, Boissinot S (2021) Mitogenomics of historical type specimens clarifies the taxonomy of Ethiopian Ptychadena Boulenger, 1917 (Anura, Ptychadenidae). ZooKeys 1070: 135-149. https://doi.org/10.3897/zookeys.1070.66598

Figure 1 Map of Ethiopia showing localities of individuals in the Ptychadena neumanni species complex used in this study. Samples with genetic data are represented by different colored circles (P. neumanni species group) or triangles (P. erlangeri species group). Stars depict the approximate type localities of P. neumanni (red), P. erlangeri (grey), and P. nana (white). A black star represents Addis Ababa, the type locality of P. largeni, a junior synonym of P. erlangeri. The approximate route of Oscar Neumann and Carlo von Erlanger's 1900 expedition in Abyssinia, during which the type specimens of all the above species were collected (except for P. largeni) is represented by a dashed line. Black arrow indicates the likely correct type locality for P. erlangeri as suggested by the authors (see Discussion).

opencc-by-4.0Nov 2021View details →
zenodo28/100

Supplementary material 1 from: Reyes-Velasco J, Goutte S, Freilich X, Boissinot S (2021) Mitogenomics of historical type specimens clarifies the taxonomy of Ethiopian Ptychadena Boulenger, 1917 (Anura, Ptychadenidae). ZooKeys 1070: 135-149. https://doi.org/10.3897/zookeys.1070.66598

Detailed guidelines for the DNA extraction from museum specimens used in this study

opencc-zeroNov 2021View details →
zenodo28/100

Figure 3 from: Reyes-Velasco J, Goutte S, Freilich X, Boissinot S (2021) Mitogenomics of historical type specimens clarifies the taxonomy of Ethiopian Ptychadena Boulenger, 1917 (Anura, Ptychadenidae). ZooKeys 1070: 135-149. https://doi.org/10.3897/zookeys.1070.66598

Figure 3 Phylogenetic inference of members of the Ptychadena neumanni species complex based on mtDNA and ddRAD-seq data A unrooted UPGMA tree of members of the P. neumanni species complex based on 2182 SNPs obtained with ddRAD-sequencing B unrooted Bayesian phylogenetic inference based on the complete mitochondrial genomes of members of the group C bayesian phylogenetic inference based on the concatenated sequences of the 12S and 16S rRNA and cox1. Black circles represent nodes with a posterior support of 1. Names in bold indicate type specimens, while stars indicate historical type specimens sequenced here and are color-coded as in Figure 1. Photographs represent members of the P. neumanni species complex; P. erlangeri (top), P. neumanni (bottom).

opencc-by-4.0Nov 2021View details →
zenodo28/100

Figure 2 from: Reyes-Velasco J, Goutte S, Freilich X, Boissinot S (2021) Mitogenomics of historical type specimens clarifies the taxonomy of Ethiopian Ptychadena Boulenger, 1917 (Anura, Ptychadenidae). ZooKeys 1070: 135-149. https://doi.org/10.3897/zookeys.1070.66598

Figure 2 Comparisons of the topologies of the mitochondrial rRNA 16S (left) and ddRAD-seq (right) for members of the Ptychadena neumanni species complex. Type specimens are indicated in red in the 16S phylogeny. Red lines indicate clades that differ in their placement between 16S and ddRAD-seq, however, the assignment of individuals to a particular species is identical between datasets. Numbers at nodes represent posterior support (pp), while black dots represent nodes with posterior support of 1.

opencc-by-4.0Nov 2021View details →
zenodo28/100

Figure 4 from: Sánchez-Vialas A, Calvo-Revuelta M, Márquez R (2017) Ptychadena in Mauritania and the first record of Ptychadena schillukorum. ZooKeys 673: 125-133. https://doi.org/10.3897/zookeys.673.10265

Figure 4 - Tomopterna cryptotis and Ptychadena schillukorum (museum number: MNCN 42214, SVL = 31.8 mm) found in syntopy, Aleg, Mauritania (photo Rafael Márquez).

opencc-by-4.0May 2017View details →
zenodo28/100

Figure 3 from: Sánchez-Vialas A, Calvo-Revuelta M, Márquez R (2017) Ptychadena in Mauritania and the first record of Ptychadena schillukorum. ZooKeys 673: 125-133. https://doi.org/10.3897/zookeys.673.10265

Figure 3 - Temporal pond where Ptychadena schillukorum was found in Aleg, Mauritania (photo Rafael Márquez).

opencc-by-4.0May 2017View details →

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