Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
325
datasets available to search
ShareScore release 0.9.0
Dataset results
325 results for “Purification”
Dataset of "Single-step Purification and Characterization of Pseudomonas aeruginosa Azurin"
<p>Azurin is a small periplasmic blue copper protein. We have created a novel approach for expressing and purifying of azurin in E. coli with high yields and optimal metalation ratio. The azurin sequence was N-terminally fused with a GST tag and protein was purified by single-step affinity chromatography on a GST-trap column. The N-terminal tag was cleaved off by HRV 3C protease and sufficient metalation was endured by incubation with copper sulphate. UV-VIS absorption, mass spectroscopy, and circular dichroism analysis all validated the effective production of azurin, appropriate protein folding and the development of an active site with an associated cofactor. MD simulations verified that incorporation of the N-terminal GPLGS segment does not affect the wild-type azurin structure.</p>
Dataset for publication "Acid leaching technology for post-consumer gypsum purification"
<p>This dataset contains underlying and extended data of publication entitled "Acid leaching technology for post-consumer gypsum purification". </p> <p>Data comprises: </p> <p>- Dataset description.txt (abbreviations and codes of samples presented in the publication)</p> <p>- Particle size analysis.zip (particle size distribution data files for the two acid leaching feedstocks in .txt format)</p> <p>- SEM.zip (scanning electron microscopy images of gypsum from refurbishment plasterboard waste before and after acid leaching in .jpg format)</p> <p>- TGA.zip (thermal gravimetric analysis raw data for gypsum from refurbishment and demolition plasterboard wastes before and after acid leaching in .xlsx format) </p> <p>- XRD.zip (X-ray diffraction data files for samples presented in the publication in .raw, .brml and .uxd formats)</p> <p>- XRF.zip (X-ray fluorescence data files for samples presented in the publication in .xlsx format)</p> <p>- Extended data.pdf (additional tables and figure supporting claims in the publication).</p>
Figure 4 in Purification and Characterization of Midgut α-Glucosidase from Larvae of the Rice Green Caterpillar, Naranga aenescens Moore
Figure 4. Irreversible thermoinactivation of the N. aenescens α-glucosidase at 35 (▲), 40 (■) and 45 °C (•). Different letters indicate that the relative activity of enzymes is significantly different from each other by Tukey's test (P <0.05).
Figure 3 in Purification and Characterization of Midgut α-Glucosidase from Larvae of the Rice Green Caterpillar, Naranga aenescens Moore
Figure 3. Effect of pH (a) and (b) temperature on the activity of N. aenescens α -glucosidase.Different letters indicate that the relative activity of enzymes is significantly different from each other by Tukey's test (P <0.05).
Figure 2 in Purification and Characterization of Midgut α-Glucosidase from Larvae of the Rice Green Caterpillar, Naranga aenescens Moore
Figure 2. Analysis of purified αglucosidase by SDS-PAGE. Lanes 1 and 2: Active fraction after ion exchange chromatography stained with histochemical and general staining, respectively; Lane 3: Molecular weight markers.
Figure 1 in Purification and Characterization of Midgut α-Glucosidase from Larvae of the Rice Green Caterpillar, Naranga aenescens Moore
Figure 1. Elution profile of N. aenescens α-glucosidase on DEAE-sepharose column. The active peak is indicated. Arrow is pointing to the fifth peak, eluted around 0.4 M salt, corresponding to the α-glucosidase activity.
Figure 2 in Recombinant - and -tubulin from Echinococcus granulosus: expression, purification and polymerization
Figure 2. Sequence alignment of Echinococcus granulosus α9-tubulin and corresponding sequences from humans and parasites. The symbol ''*'' denotes the positions of amino acids that have a single, fully conserved amino acid residue; the symbol '':'' denotes conservation between groups of amino acids with strongly similar properties; the symbol ''.'' denotes conservation between groups of amino acids with weakly similar properties; and the symbol ''–'' denotes gaps inserted to maximize sequence alignment. EgA9, E. granulosus α9; HuA6, human alpha 6 (119578461); HmA5, H. microstoma alpha 5 (674586714); HcA, H. contortus alpha tubulin (159155); SjA1, S. japonicum alpha 1–3 (226478902); and TgA, T. gondii alpha (161937).
Figure 1 in Recombinant - and -tubulin from Echinococcus granulosus: expression, purification and polymerization
Figure 1. The cloning and expression of α9- and β4-tubulin. (a) The expression of α9- and β4-tubulin in E. coli BL21 (DE3). M: standard protein molecular weight marker, lane 1: negative control without induction, lane 2: induced control, lane 3: the supernatant after sonication, lane 4: the pellet after sonication, and lane 5: purified recombinant tubulin. (b) Western blot analysis. M: standard protein molecular weight marker, lane 1: α-tubulin, lane 2: β-tubulin.
Figure 3 in Recombinant - and -tubulin from Echinococcus granulosus: expression, purification and polymerization
Figure 3. The sequence alignment of Echinococcus granulosus β4-tubulin and corresponding sequences from humans and parasites. Symbol meanings are as in Figure 2. EgB4, E. granulosus β4-tubulin; EmB1, E. multilocularis Tub-1 gene (7838198); EmB2, E. multilocularis Tub-2 gene (7838200); EmB3, E. multilocularis Tub-3 gene (7838202); HuB3, human B3 (50592996); HuB4, human B4a (574584803); HmB2C, H. microstoma beta 2C (674589300); HcB1, H. contortus beta tubulin isotype 1 (124244617); SjB2, S. japonicum beta 2 (226467271); PfB, P. falciparum beta (160732).
Figure 5 in Recombinant - and -tubulin from Echinococcus granulosus: expression, purification and polymerization
Figure 5. The polymerization of Echinococcus granulosus α9- and β4-tubulin. (a) Spectrophotometric analysis of polymerization of E. granulosus α9- and β4-tubulin at different concentrations. (b) Confocal scanning laser micrographs of the polymerization product of recombinant tubulin showing immune reactivity to anti-α-tubulin/Alexa-Fluor 488 antibody and anti-β-tubulin/Alexa-Fluor 647 antibody. (I): Phase contrast view, (II): α-tubulin immuno-reactivity, (III): β-tubulin immune-reactivity. (c) The microtubule-like structure resulting from polymerization of pure expressed α9- and β4-tubulin under appropriate conditions in vitro.
Figure 4 in Recombinant - and -tubulin from Echinococcus granulosus: expression, purification and polymerization
Figure 4. Modelled structure of the polymerized Echinococcus granulosus α9- and β4-tubulin dimer based on PDB ID 4f6r.
Full-length HTT purification from HEK293T suspension culture using baculovirus transduction protocol for over-expression 2018/04/02
<p>Structure-function open lab notebook project. Full-length HTT purification from HEK293T suspension culture using baculovirus transduction protocol for over-expression 2018/04/02.</p>
Dataset HPLC purification improves 68Ga-DOTA-NAPamide
<p>Numerical data for the manuscript "<strong>Increasing molar activity by HPLC purification improves <sup>68</sup>Ga-DOTA-NAPamide tumor accumulation in a B16/F1 melanoma xenograft model</strong>"</p>
GAPDH Purification
<p>Huntington’s Disease (HD) is a hereditary neurodegenerative disease; the cause of which is due to a CAG repeat extension in the HTT Gene. This extension is then translated into an elongation of exon 1 which is primarily composed of a disordered PolyQ repeat. Although the cause is known, the mechanism by which this extension affects the function of Huntingtin (HTT), the protein produced by the HTT Gene, has yet to be fully understood. A part of this difficulty is our lack of understanding of the role of normal HTT in our cells. To further our understanding of the role of HTT, we at the SGC have set out to explore the HTT interactome, which will give us a better idea of what specific interactions are modulated in HD. Our first step on this journey was to conduct a literature review alongside a BioID experiment to come up with a list of putative huntingtin interaction partners. One of the hits on the list was GAPDH. This means that we must obtain a pure sample of GAPDH for use in future experiments to further validate the claim that these proteins interact.</p>
Techno Economic Analysis of Biogas Purification by Methane and Acetate Manufacturing CO2 to CH4 2024 SuppInfo
<p>Techno Economic Analysis calculations for manuscript of Biogas Purification by Methane and Acetate Manufacturing to convert CO2 to CH4: Wastewater treatment plants have two persistent financial and energetic drains, the carbon dioxide content of biogas, which limits its commercial sale, and the presence of trace organics in the wastewater effluent, which damages the aquatic ecosystem. Biogas is a renewable methane resource that is underutilized due to the variable CO2 content (~40%). Biogas is energy intensive to purify and limited by the economy of scale (>8.85 GJ/hour) to large-scale purification methods, thus small-scale processes require development. Electrocatalytic microbes native to wastewater have been shown to convert CO2 to CH4 and acetate, however complete conversion of the CO2 content to CH4 is energy intensive. Here we show a low power bioelectrochemical fuel cell design to purify biogas to pipeline quality methane (98%), manufacture methane and/or acetate, and remove trace organics, using HCO3- as the transport charge carrier from dissolved CO2 from the biogas through an anion exchange membrane. This decreased the power required to separate CO2 from methane in biogas on a molar basis, resulting in a net energy recovery similar to current industrial systems. Magnesium anode use resulted in an energy positive system. Tests evaluated the influence of cathode potential on the current density, HCO3- ion flux and the rates and efficiencies of methane production, resulting in optimization at -0.7V vs Standard Hydrogen Electrode (SHE). A techno-economic analysis modeled a positive return on investment for scaled-up production to purify small biogas streams that are otherwise financially unrecoverable. Carbon sequestration by production of methane, acetate and solid fertilizers demonstrated profitable and energy efficient waste-to-resource conversion.</p>
Ethics: Purification of the Soul | Islam and Muslim Civilizations | iBrary
<p>This video introduces the module “Ethics: Purification of the Soul” from the course Islam and Muslim Civilizations, taught by Prof. Shafique N. Virani. You can download the complete online course, including this module, for free from <a href="https://www.youtube.com/redirect?event=video_description&redir_token=QUFFLUhqa2xPSmF1azkydjFmejdBZjhZN014eXlmYkVhQXxBQ3Jtc0ttZVhNR1ExSFZFV2dkWGVGSERBaE5GcHlJb2plM0RpamdLQUgzSUVzMV9WMWtvZDFRdHZnWjNsb2trNmU3NlJZSEt0NGlwbHkxLXNGZUJmSnRneElXVF9VdER6R2p2dk1sMm9vS05XeUJ0dFNDaThDVQ&q=https%3A%2F%2Fwww.ecampusontario.ca%2F&v=FYCDjMkTrrg">https://www.ecampusontario.ca/</a>.</p>
Production and purification of receptor-binding domain (RBD) of the Spike protein from a transiently transfected mammalian cell
<p> </p> <p>Production and purification of recombinant receptor-binding domain (RBD) of the Spike protein from a transiently transfected EXPI293 mammalian cell .</p>
Production and purification protocols for Ectodomain of SARS-CoV-2 Spike (S) protein
<p>Production and purification protocols for Ectodomain of SARS-CoV-2 Spike (S) protein </p>
Attitude Towards Water Purification Technology
<p>Dataset of <em>Sustainability Psychology of Disruption: Attitude towards Water Purification Technology can be predicted by Cultural Value Orientation and Personality Traits</em>. Adding a worksheet, CFA - Instrument Validation. Revision the average score of Openness.</p>
Raw and processed data for 'Purification-based quantum error mitigation of pair-correlated electron simulations'
<p>Experimental data for 'Demonstration of purification-based error mitigation for quantum simulation'</p> <p>This directory contains two folders - 'final_data_sets' and 'plots_for_paper'.<br> 'final_data_sets' contains directories with raw experimental data, either in the form of raw shots, or accumulated expectation values. Everything is stored in either json or pickle format. This data was processed to generate the datasets in subfolders of 'plots_for_paper', which also contains notebooks for generating plots in the paper (data processing scripts to appear soon). Note that the python file to generate Fig.3 is in 'plots_for_paper/paper_plot_ring_opening_sixq_10q'.</p> <p>For any further questions, please email teobrien@google.com.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.