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Figure 3 in Mites associated with egg masses of the viburnum leaf beetle Pyrrhalta viburni (Paykull) on Viburnum tinus L.

Figure 3 Timing of mite infestation experiment 2017. (a) abundance and (b) diversity of mites found on V. tinus twigs depending on the number of intactP. viburni egg masses present on the twigs (mean ± SE). Data are pooled in three categories: twigs with 0 to 4 intact egg masses (n = 81), twigs with 5 to 9 intact egg masses (n = 13), and twigs with ≥ 10 intact egg masses (n = 8); (c) abundance of mites found onV. tinus twigs depending on the number of damagedP. viburni egg masses present on the twigs. Data are pooled in three categories: twigs with 0 to 4 damaged egg masses (n = 62), twigs with 5 to 9 damaged egg masses (n = 24), and twigs with ≥ 10 damaged egg masses (n = 16).

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Figure 2 Observational study 2016 in Mites associated with egg masses of the viburnum leaf beetle Pyrrhalta viburni (Paykull) on Viburnum tinus L.

Figure 2 Observational study 2016. (a) abundance and (b) diversity of mites foundV. tinus on twigs depending onP. viburni infestation: twigs

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Figure 1 in Mites associated with egg masses of the viburnum leaf beetle Pyrrhalta viburni (Paykull) on Viburnum tinus L.

Figure 1 Pictures of (a) four intact Pyrrhalta viburni egg masses along a Viburnum tinus twig, with the protective "egg cap" visible; (b) one damagedP. viburni egg mass following plant wounding response, with the egg cap removed and the egg mass cavity partially covered by wounding tissue; (c) twoP. viburni eggs; (d) Detritivorous miteTrichoribates trimaculatus nymph; (e) T. trimaculatus adult; (f) predatory mite Anystis baccarum. Photo credit: (a)(b) Gaylord Desurmont; (c)(d)(e)(f) Elven Kerdellant.

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Table 4 in Mites associated with egg masses of the viburnum leaf beetle Pyrrhalta viburni (Paykull) on Viburnum tinus L.

<p><b>Table 4</b> Factors affecting (a) total mite abundance, (b) phytophagous/detritivorous mite abundance, (c) predaceous mite abundance, and (d) diversity of mite morphotypes on <i>Viburnum tinus</i> twigs artificially infested with <i>Pyrrhalta viburni</i> egg masses (ANOVA, &alpha; = 0.05) during the timing of mite infestation experiment (2016-2017). Bold values indicate significant effects.</p><table><tbody><tr><th>(a) Total mite abundance</th><th></th><th>df</th><th>F-value</th><th><i>P</i> -value</th></tr></tbody><tbody><tr><th></th><td></td><td>8, 93</td><td>5.84</td><td><b>&lt;0.0001</b></td></tr><tr><th>Effects tested</th><td>Time of collection</td><td>2</td><td>6.04</td><td><b>&lt;0.01</b></td></tr><tr><th></th><td>Site</td><td>3</td><td>1.73</td><td>0.16</td></tr><tr><th></th><td>Twig length (cm)</td><td>1</td><td>0.62</td><td>0.43</td></tr><tr><th></th><td>Number of intact egg masses</td><td>1</td><td>13.85</td><td><b>&lt;0.001</b></td></tr><tr><th></th><td>Number of damaged egg masses</td><td>1</td><td>6.73</td><td><b>0.01</b></td></tr><tr><th>(b) Phytophagous/Detritivorous mite abundance</th><td>df</td><td>F-value</td><td><i>P</i> -value</td></tr><tr><th></th><td></td><td>8, 93</td><td>3.52</td><td><b>&lt;0.01</b></td></tr><tr><th>Effects tested</th><td>Time of collection</td><td>2</td><td>5.53</td><td><b>&lt;0.01</b></td></tr><tr><th></th><td>Site</td><td>3</td><td>1.88</td><td>0.14</td></tr><tr><th></th><td>Twig length (cm)</td><td>1</td><td>0.2</td><td>0.65</td></tr><tr><th></th><td>Number of intact egg masses</td><td>1</td><td>3.39</td><td>0.07</td></tr><tr><th></th><td>Number of damaged egg masses</td><td>1</td><td>1.19</td><td>0.27</td></tr><tr><th>(c) Predaceous mite abundance</th><td></td><td>df</td><td>F-value</td><td><i>P</i> -value</td></tr><tr><th></th><td></td><td>10, 89</td><td>3.38</td><td><b>&lt;0.01</b></td></tr><tr><th>Effects tested</th><td>Time of collection</td><td>2</td><td>0.61</td><td>0.54</td></tr><tr><th></th><td>Site</td><td>3</td><td>1.74</td><td>0.16</td></tr><tr><th></th><td>Twig length (cm)</td><td>1</td><td>0.58</td><td>0.44</td></tr><tr><th></th><td>Number of intact egg masses</td><td>1</td><td>15.54</td><td><b>&lt;0.001</b></td></tr><tr><th></th><td>Number of damaged egg masses</td><td>1</td><td>8.52</td><td>&lt;<b>0.01</b></td></tr><tr><th>(d) Total mite diversity</th><td></td><td>df</td><td>F-value</td><td><i>P</i> -value</td></tr><tr><th></th><td></td><td>10, 89</td><td>3.93</td><td><b>&lt;0.001</b></td></tr><tr><th>Effects tested</th><td>Time of collection</td><td>2</td><td>1.04</td><td>0.36</td></tr><tr><th></th><td>Site</td><td>3</td><td>3.27</td><td><b>0.02</b></td></tr><tr><th></th><td>Twig length (cm)</td><td>1</td><td>0.52</td><td>0.47</td></tr><tr><th></th><td>Number of intact egg masses</td><td>1</td><td>13.07</td><td><b>&lt;0.001</b></td></tr><tr><th></th><td>Number of damaged egg masses</td><td>1</td><td>2.98</td><td>0.09</td></tr></tbody></table>

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Table 2 in Mites associated with egg masses of the viburnum leaf beetle Pyrrhalta viburni (Paykull) on Viburnum tinus L.

<p><b>Table 2</b> List of the mite species found on <i>Viburnum tinus</i> twigs during the study (sp. = species).</p><table><tbody><tr><th><b>Feeding guild</b></th><th><b>Family</b></th><th><b>Genus</b></th><th><b>Species</b></th></tr></tbody><tbody><tr><th>Predaceous</th><td>Phytoseiidae</td><td><i>Typhlodromus</i> (<i>Typhlodromus</i>)</td><td><i>T. phialatus</i></td></tr><tr><th></th><td></td><td><i>Typhlodromus</i> (<i>Anthoseius</i>)</td><td><i>T. recki</i></td></tr><tr><th></th><td></td><td></td><td><i>T. rhenanoides</i></td></tr><tr><th></th><td></td><td><i>Euseius</i></td><td><i>E. gallicus</i></td></tr><tr><th></th><td></td><td><i>Kampimodromus</i></td><td><i>K. aberrans</i></td></tr><tr><th></th><td></td><td><i>Amblyseius</i></td><td>sp.</td></tr><tr><th></th><td>Cunaxidae</td><td><i>Neocunaxoides</i></td><td>sp.</td></tr><tr><th></th><td>Cheyletidae</td><td><i>Cheletogenes</i></td><td><i>C. ornatus</i></td></tr><tr><th></th><td>Anystidae</td><td><i>Anystis</i></td><td><i>A. baccarum</i></td></tr><tr><th>Phytophagous</th><td>Tetranychidae</td><td><i>Tetranychus</i></td><td>sp. (<i>T</i>. <i>urticae</i> group)</td></tr><tr><th></th><td>Tenuipalpidae</td><td><i>Brevipalpus</i></td><td>sp.</td></tr><tr><th>Detritivorous</th><td>Ceratozetidae</td><td><i>Trichoribates</i></td><td><i>T. trimaculatus</i></td></tr><tr><th></th><td>Micreremidae</td><td><i>Micreremus</i></td><td><i>M. brevipes</i></td></tr><tr><th></th><td>Camisiidae</td><td><i>Camisia</i></td><td><i>C. segnis</i></td></tr><tr><th></th><td>Cymbaeremaeidae</td><td><i>Scapheremaus</i></td><td><i>S. patella</i></td></tr><tr><th></th><td>Acaridae</td><td><i>Tyrophagus</i></td><td><i>T. putrescenciae</i></td></tr><tr><th></th><td>Winterschmidtiidae</td><td><i>Calvolia</i></td><td>sp.</td></tr><tr><th></th><td>Tydeidae</td><td>unknown</td><td>sp.</td></tr></tbody></table>

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Table 3 in Mites associated with egg masses of the viburnum leaf beetle Pyrrhalta viburni (Paykull) on Viburnum tinus L.

<p><b>Table 3</b> Factors affecting the (a) abundance of mites and (b) diversity of mite morphotypes present on <i>Viburnum tinus</i> twigs infested with <i>Pyrrhalta viburni</i> egg masses (Generalized Linear Model, poisson distribution, &alpha; = 0.05) during an observational study (2016). Bold values indicate significant effects.</p><table><tbody><tr><th>(a) Mite abundance</th><th>df</th><th>&Chi;&sup2;-value</th><th><i>P</i> -value</th></tr></tbody><tbody><tr><th>Full model</th><td></td><td>9, 90</td><td>49.77</td><td><b>&lt;0.0001</b></td></tr><tr><th>Effects tested</th><td>Site</td><td>4</td><td>6.03</td><td>0.2</td></tr><tr><th></th><td>Twig length (cm)</td><td>1</td><td>4.09</td><td><b>0.04</b></td></tr><tr><th></th><td>Shrub infestation</td><td>2</td><td>0.91</td><td>0.63</td></tr><tr><th></th><td>Number of intact egg masses</td><td>1</td><td>16.22</td><td><b>&lt;0.0001</b></td></tr><tr><th></th><td>Number of damaged egg masses</td><td>1</td><td>0.37</td><td>0.54</td></tr><tr><th>(b) Mite diversity</th><td></td><td>df</td><td>&Chi;&sup2;-value</td><td><i>P</i> -value</td></tr><tr><th>Full model</th><td></td><td>9, 90</td><td>53.06</td><td><b>&lt;0.0001</b></td></tr><tr><th>Effects tested</th><td>Site</td><td>5</td><td>8.5</td><td>0.07</td></tr><tr><th></th><td>Twig length (cm)</td><td>1</td><td>0.77</td><td>0.38</td></tr><tr><th></th><td>Shrub infestation</td><td>2</td><td>8.6</td><td><b>0.01</b></td></tr><tr><th></th><td>Number of intact egg masses</td><td>1</td><td>28.88</td><td><b>&lt;0.0001</b></td></tr><tr><th></th><td>Number of damaged egg masses</td><td>1</td><td>1.59</td><td>0.2</td></tr></tbody></table>

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Table 1 in Mites associated with egg masses of the viburnum leaf beetle Pyrrhalta viburni (Paykull) on Viburnum tinus L.

<p><b>Table 1</b> Coordinates of the study sites used for the observational study (2016) and the timing of mite infestation experiment (2017).</p><table><tbody><tr><th><b>Observational study 2016</b></th><th><b>Coordinates (Latitude, Longitude)</b></th></tr></tbody><tbody><tr><th>Site 1</th><td>43.683325, 3.874779</td></tr><tr><th>Site 2</th><td>43.667094, 3.851299</td></tr><tr><th>Site 3</th><td>43.769778, 3.787764</td></tr><tr><th>Site 4</th><td>43.716070, 3.848204</td></tr><tr><th>Site 5</th><td>43.681688, 3.878901</td></tr><tr><th><b>Timing mite infestation 2017</b></th></tr><tr><th>Site 1</th><td>43.682444, 3.880156</td></tr><tr><th>Site 2</th><td>43.676900, 3.874368</td></tr><tr><th>Site 3</th><td>43.714209, 3.861153</td></tr><tr><th>Site 4</th><td>43.708495, 3.837012</td></tr></tbody></table>

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FIGURES 31–36. Male habitus. 31 in Revision of Pyrrhalta (Coleoptera: Chrysomelidae: Galerucinae) species with maculate elytra

FIGURES 31–36. Male habitus. 31—Pyrrhalta carolusi sp. nov. (holotype, 4.3 mm); 32—P. kaboureki sp. nov. (holotype, 3.9 mm); 33—P. kambaitiensis sp. nov. (holotype, 4.5 mm); 34—P. lucka sp. nov. (holotype, 3.8 mm); 35—P. schillhammeri sp. nov. (holotype, 3.7 mm); 36—P. tsoui sp. nov. (paratype, 3.9 mm).

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FIGURES 25–30. 25–27. Pyrrhalta maculata. 25 in Revision of Pyrrhalta (Coleoptera: Chrysomelidae: Galerucinae) species with maculate elytra

FIGURES 25–30. 25–27. Pyrrhalta maculata. 25—holotype (male, 4.5 mm); 26—holotype (labels); 27—male (Taiwan, 4.3 mm). 28–30. Unidentified females. 28—female from Sichuan (4.3 mm); 29—female from Sichuan (3.9 mm); 30—female from Yunnan (4.5 mm).

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FIGURES 8–21 in Revision of Pyrrhalta (Coleoptera: Chrysomelidae: Galerucinae) species with maculate elytra

FIGURES 8–21. Details of Pyrrhalta males. 8—P. kaboureki (last abdominal ventrite); 9—P. lucka sp. nov. (last abdominal ventrite); 10—P. lucka sp. nov. (protarsus, lateral view); 11—P. schillhammeri sp. nov. (protarsus, lateral view); 12—P. carolusi sp. nov. (mesotarsus, lateral view); 13—P. kaboureki sp. nov. (mesotarsus, lateral view); 14—P. kambaitiensis sp. nov. (mesotarsus, lateral view); 15—P. lucka sp. nov. (mesotarsus, lateral view); 16—P. schillhammeri sp. nov. (mesotarsus, lateral view); 17—P. kaboureki (metatarsus, dorsal view); 18—P. maculata (antenna, dorsal and lateral views); 19—P. carolusi sp. nov. (antenna, dorsal and lateral views); 20—P. lucka sp. nov. (antenna, dorsal view); 21—P. schillhammeri sp. nov. (antenna, dorsal view). Scale bars: 1.0 mm for Figs 8–9, 0.5 mm for Figs 10–21.

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FIGURES 1–7. Aedeagus. 1 in Revision of Pyrrhalta (Coleoptera: Chrysomelidae: Galerucinae) species with maculate elytra

FIGURES 1–7. Aedeagus. 1—Pyrrhalta maculata (basal half in dorsal view, apical half in dorsal view, lateral view); 2—P. carolusi sp. nov. (dorsal and lateral views); 3—P. kaboureki sp. nov. (dorsal, lateral and ventral views); 4—P. kambaitiensis sp. nov. (basal part in dorsal view, apex in dorsal view, lateral view); 5—P. lucka sp. nov. (dorsal and lateral views); 6—P. schillhammeri sp. nov. (dorsal and lateral views); 7—P. tsoui sp. nov. (basal part in dorsal view, apex in dorsal view, lateral view). Scale bar: 0.5 mm.

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FIGURES 22–24. Spermatheca. 22 in Revision of Pyrrhalta (Coleoptera: Chrysomelidae: Galerucinae) species with maculate elytra

FIGURES 22–24. Spermatheca. 22—Pyrrhalta maculata; 23—P. lucka sp. nov.; 24—P. tsoui sp. nov. Scale bar: 0.2 mm.

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Fig. 1 in Distribution of Two Invasive Leaf Beetles,Pyrrhalta viburni(Paykull) andLilioceris lilii(Scopoli) (Coleoptera: Chrysomelidae), in Washington State

Fig. 1. Collection localities for the viburnum leaf beetle, Pyrrhalta viburni, from 2004 through 2015 and the lily leaf beetle, Lilioceris lilii, from 2013 to 2015 in western Washington. The single 2016 collection of P. viburni from Spokane County is not figured.

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Figures 6-8 from: Matsumura Y, Suenaga H, Kamimura Y, Gorb SN (2017) Traumatic mating by hand saw-like spines on the internal sac in Pyrrhalta maculicollis (Coleoptera, Chrysomelidae, Galerucinae). In: Chaboo CS, Schmitt M (Eds) Research on Chrysomelidae 7. ZooKeys 720: 77-89. https://doi.org/10.3897/zookeys.720.13015

Figures 6-8 - The internal sac membrane and spines of Pyrrhalta maculicollis. 6, 7 the sample was cut laterally, opened, dehydrated, and dried at the critical point 8 the sample dried at room temperature. The surfaces of the spines is slightly shrunken in comparison to that depicted in 7. Abbreviations: mp, membranous projections; s, spines; each is highlighted with blue and pink, respectively.

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Figures 17-25 from: Matsumura Y, Suenaga H, Kamimura Y, Gorb SN (2017) Traumatic mating by hand saw-like spines on the internal sac in Pyrrhalta maculicollis (Coleoptera, Chrysomelidae, Galerucinae). In: Chaboo CS, Schmitt M (Eds) Research on Chrysomelidae 7. ZooKeys 720: 77-89. https://doi.org/10.3897/zookeys.720.13015

Figures 17-25 - Female reproductive systems: 17–19 Pyrrhalta maculicollis 20–22 P. tibialis 23 P. humeralis 24–25 Tricholochmaea semifulva species complex. 18, 21, 23, 25 show the cross sections of the lower part of the vagina, where wounds were found in P. maculicollis; each cuticular, epidermal, and muscular layer are partly highlighted in the upper right corner. 19, 22 show enlarged parts of the cuticular, epidermal, and muscular layers; these samples were embedded in glycerine for two days before taking the images. Arrowheads in 22 point to the strip stained with toluidine blue. Abbreviations: c, cuticle; e, epidermis; m, muscles; sc, spermathecal capsule.

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Figures 1-5 from: Matsumura Y, Suenaga H, Kamimura Y, Gorb SN (2017) Traumatic mating by hand saw-like spines on the internal sac in Pyrrhalta maculicollis (Coleoptera, Chrysomelidae, Galerucinae). In: Chaboo CS, Schmitt M (Eds) Research on Chrysomelidae 7. ZooKeys 720: 77-89. https://doi.org/10.3897/zookeys.720.13015

Figures 1-5 - Aedeagus of Pyrrhalta maculicollis in the lateral view 1 relative size of the aedeagus compared to body size 2 the aedeagus and a part of the ejaculatory duct, muscles completely macerated. Two arrows point to the membranous areas of the median lobe 3 a micro CT scanned and segmented aedeagus and a part of the ejaculatory duct. The green structure represents the part of the ejaculatory duct with well–developed circular muscles, the yellow one represents the median lobe, and the red ones represent spines on the internal sac 4 the internal sac at rest with a part of the ejaculatory duct (ed) 5 schemes of the aedeagus at rest (left) and with partly evaginated internal sac during copulation (right). Abbreviations: aed, aedeagus; ed, ejaculatory duct; is, internal sac; ml, median lobe.

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Figures 13-16 from: Matsumura Y, Suenaga H, Kamimura Y, Gorb SN (2017) Traumatic mating by hand saw-like spines on the internal sac in Pyrrhalta maculicollis (Coleoptera, Chrysomelidae, Galerucinae). In: Chaboo CS, Schmitt M (Eds) Research on Chrysomelidae 7. ZooKeys 720: 77-89. https://doi.org/10.3897/zookeys.720.13015

Figures 13-16 - Schemes of hypothetical couplings between male and female genitalia, light microscopy (LM) and scanning electron microscopy (SEM) images of the male internal sac 13 Pyrrhalta maculicollis 14 P. tibialis 15 P. humeralis 16 Tricholochmaea semifulva species complex. The schemes were created using information on morphology and dimensions of both male and female genitalia, but the genital coupling has not been experimentally proven. The meshed areas in the male internal sacs show areas covered by tiny projections, as shown in the SEM images. All samples were dried at room temperature. Abbreviations: is, internal sac; mp, membranous projection; s, sclerite; sc, spermathecal capsule; sd, spermathecal duct; v, vagina.

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Figures 9-12 from: Matsumura Y, Suenaga H, Kamimura Y, Gorb SN (2017) Traumatic mating by hand saw-like spines on the internal sac in Pyrrhalta maculicollis (Coleoptera, Chrysomelidae, Galerucinae). In: Chaboo CS, Schmitt M (Eds) Research on Chrysomelidae 7. ZooKeys 720: 77-89. https://doi.org/10.3897/zookeys.720.13015

Figures 9-12 - Wounds observed on the vagina of Pyrrhalta maculicollis 9 schemes of three female vaginas, which had largest numbers of wounds, are shown. Each symbol represents one individual 10 one representative with four wounds 11, 12 enlarged images of the wounds. Abbreviations: sc: spermathecal capsule.

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Figures 1-12 from: Yang X, Nie R, Zhou D, Xue H (2013) Notes on black elytron species of Pyrrhalta Joannis and the description of a new species from China (Coleoptera, Chrysomelidae, Galerucinae). ZooKeys 289: 41-56. https://doi.org/10.3897/zookeys.289.4266

Figures 1-12 - Habitus. 1–2 Pyrrhalta fossata (holotype) 3–4 Pyrrhalta huangshana (holotype) 5–6 Pyrrhalta martensi (paratype) 7–8 Pyrrhalta meghalayana (paratype) 9–10 Pyrrhalta metallica 11–12 Pyrrhalta orientalis (holotype).

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Figures 36-43 from: Yang X, Nie R, Zhou D, Xue H (2013) Notes on black elytron species of Pyrrhalta Joannis and the description of a new species from China (Coleoptera, Chrysomelidae, Galerucinae). ZooKeys 289: 41-56. https://doi.org/10.3897/zookeys.289.4266

Figures 36-43 - Aedeagus / spermatheca 36–37 Pyrrhalta sulcatipennis (holotype, 36 dorsal view 37 lateral view) 38–39 Pyrrhalta tianmuensis (38 dorsal view 39 lateral view) 40–41 Pyrrhalta warchalowskii (Bezděk 2007 orig., 40 dorsal view 41 lateral view) 42 spermatheca of Pyrrhalta fossata (holotype) 43 spermatheca of Pyrrhalta xizangana (paratype).

opencc-by-4.0Apr 2013View details →

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