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16 results for “Quarantine pest”
Development of thrips barcode database and multiplex real-time PCR assay for quarantine and agriculture pest species
<p>Thrips (Order Thysanoptera) species are agriculturally important as plant sap sucking pests and vectors of several plant diseases. They are very small insects and commonly associated with imported commodities at New Zealand border in all life stages. Morphological identification of thrips is mainly performed on adults, but the available identification keys for immature stages do not include many species and are inadequate, thus DNA barcode was regularly used for thrips identification, here, we have generated DNA barcode data for over 29 thrips species from over 100 individuals. At New Zealand border,<em> Frankliniella occidentalis </em>is the dominant species intercepted, followed by <em>F. panamensis</em>, <em>Thrips palmi</em> and <em>T. tabaci </em>and several other thrips species. Hence, we have also developed a multiplex real time PCR assay, targeting the four thrips species to facilitate the identification of quarantine interceptions with more accurate and faster diagnostic method for any developmental stages. The DNA barcode database further assists in thrip identification. The assay showed high specificity for all the four target species and could detect 10 copies/ µL of the target DNA. Linear responses and high correlation coefficients between the amount of DNA and <em>C</em><sub>q</sub> values for each species were also achieved. The method was tested on single egg, larva and adult and proved to be applicable for all life stages of the four species. This study has demonstrated the assay is a useful biosecurity tool for rapid and reliable identification of the target thrips species. </p>
Рис. 9. Связь межΔу чисΛенностью и проΔуктивностью самок Heterodera glycines Fig. 9. Relationship between the number and productivity of Heterodera glycines females in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 9. Связь межΔу чисΛенностью и проΔуктивностью самок Heterodera glycines Fig. 9. Relationship between the number and productivity of Heterodera glycines females
Рис. 7. Сезонная Δинамика соΔержимого цист H. glycines Fig. 7. Seasonal dynamics of H. glycines cysts content in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 7. Сезонная Δинамика соΔержимого цист H. glycines Fig. 7. Seasonal dynamics of H. glycines cysts content
Рис. 6. Изменение ΔоΛи цист Heterodera glycines разных цветовых групп на протяжении сезона размножения. ГраΔации цвета цист: 1 — моΛочный, 2 — жеΛто- и светΛо-коричневый, 3 — коричневый, 4 — каштановый и темно-коричневый in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 6. Изменение ΔоΛи цист Heterodera glycines разных цветовых групп на протяжении сезона размножения. ГраΔации цвета цист: 1 — моΛочный, 2 — жеΛто- и светΛо-коричневый, 3 — коричневый, 4 — каштановый и темно-коричневый
Рис. 10. РаспреΔеΛение обсΛеΔованных поΛей в иссΛеΔуемом регионе по степени засоренности Fig. 10. Distribution of the surveyed fields in the studied region by the degree of field weediness in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 10. РаспреΔеΛение обсΛеΔованных поΛей в иссΛеΔуемом регионе по степени засоренности Fig. 10. Distribution of the surveyed fields in the studied region by the degree of field weediness
Рис. 1. Àинамика посевных пΛощаΔей сои в Приморском крае в 1996–2018 гг. (по Δанным Àепартамента сеΛьского хозяйства и проΔовоΛьствия Приморского края) Fig. 1. The dynamic of soybean crop area at Primorsky Region in 1996–2018 (based on data from the Department of Agriculture and provision of the Primorsky Region) in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 1. Àинамика посевных пΛощаΔей сои в Приморском крае в 1996–2018 гг. (по Δанным Àепартамента сеΛьского хозяйства и проΔовоΛьствия Приморского края) Fig. 1. The dynamic of soybean crop area at Primorsky Region in 1996–2018 (based on data from the Department of Agriculture and provision of the Primorsky Region)
Рис. 5. Зависимость межΔу цветом и размером цист Heterodera glycines. ГраΔации цвета цист: 1 — моΛочный, 2 — жеΛтый и светΛо-коричневый, 3 — коричневый, 4 — каштановый, 5 — темно-коричневый. Размер цист — в баΛΛах Fig. 5. Relationship between the color and size of Heterodera glycines cysts. Color gradations of cysts: 1 — milky, 2 — yellow and light brown, 3 — brown, 4 — chestnut, 5 — dark brown. The size of cysts — in classes in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 5. Зависимость межΔу цветом и размером цист Heterodera glycines. ГраΔации цвета цист: 1 — моΛочный, 2 — жеΛтый и светΛо-коричневый, 3 — коричневый, 4 — каштановый, 5 — темно-коричневый. Размер цист — в баΛΛах Fig. 5. Relationship between the color and size of Heterodera glycines cysts. Color gradations of cysts: 1 — milky, 2 — yellow and light brown, 3 — brown, 4 — chestnut, 5 — dark brown. The size of cysts — in classes
Рис. 8. Изменение жизнеспособности цист Heterodera glycines на протяжении сезона размножения Fig. 8. Changes in the viability of Heterodera glycines cysts during the breeding season in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 8. Изменение жизнеспособности цист Heterodera glycines на протяжении сезона размножения Fig. 8. Changes in the viability of Heterodera glycines cysts during the breeding season
Рис. 4. Сезонная Δинамика размеров цист Heterodera glycines и чисΛа яиц в них. Размер цист в баΛΛах: 1 — меΛкие, 2 — среΔние, 3 — крупные Fig. 4. Seasonal dynamics of the size of Heterodera glycines cysts and the number of eggs in them. The size of cysts in classes: 1 — small, 2 — medium, 3 — large in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 4. Сезонная Δинамика размеров цист Heterodera glycines и чисΛа яиц в них. Размер цист в баΛΛах: 1 — меΛкие, 2 — среΔние, 3 — крупные Fig. 4. Seasonal dynamics of the size of Heterodera glycines cysts and the number of eggs in them. The size of cysts in classes: 1 — small, 2 — medium, 3 — large
Рис. 11. Зависимость чисΛенности Heterodera glycines от засоренности поΛей Fig. 11. Dependence of the number of Heterodera glycines on the field weediness in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 11. Зависимость чисΛенности Heterodera glycines от засоренности поΛей Fig. 11. Dependence of the number of Heterodera glycines on the field weediness
Fig. 6 in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Fig. 6. Changes in the proportion of Heterodera glycines cysts of different color groups during the breeding season. Color gradations of cysts: 1 — milk, 2 — yellow and light brown, 3 — brown, 4 — chestnut and dark brown
Рис. 3. ЧисΛо яиц в цистах Heterodera glycines разных размерных групп в 2018 и 2019 гг. Fig. 3. Number of eggs in cysts of Heterodera glycines of different size groups in 2018 and 2019 in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 3. ЧисΛо яиц в цистах Heterodera glycines разных размерных групп в 2018 и 2019 гг. Fig. 3. Number of eggs in cysts of Heterodera glycines of different size groups in 2018 and 2019
Рис. 2. РаспреΔеΛение среΔних почвенных образцов по коΛичеству жизнеспособных цист Heterodera glycines Fig. 2. Distribution of average soil samples by the number of viable cysts of Heterodera glycines in Reproductive potential of Soybean Cyst Nematode Heterodera glycines - quarantine pest of soybean - in Primorsky Region conditions
Рис. 2. РаспреΔеΛение среΔних почвенных образцов по коΛичеству жизнеспособных цист Heterodera glycines Fig. 2. Distribution of average soil samples by the number of viable cysts of Heterodera glycines
Fine-scale invasion genetics of the quarantine pest, Anoplophora glabripennis, reconstructed in single outbreaks
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Supplementary material 1 from: Saravanakumar D, Bartholomew ES, Seepersad G, Gore-Francis J, Goldsmith J, Ramnanan N, Chang PG, Bridgemohan P, Sewsaran R, Medrano-Cabral S, Morrison StS (2023) Prioritisation of quarantine pest list for the Caribbean using a multi-criteria decision approach. NeoBiota 88: 1-16. https://doi.org/10.3897/neobiota.88.102673
AHP model developed for priotization of quarantine plant pests in the Caribbeean
Data from: Species limits, quarantine risk and the intrigue of a polyphagous invasive pest with highly restricted host relationships in its area of invasion
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