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6 results for “RECQL5”
Human RECQL5 helicase; A Target Enabling Package
<p>RECQL5 is a member of the RecQ family of helicase which have important functions in DNA repair pathways and maintenance of genome integrity. RECQL5 has recently been identified as a synthetic lethal candidate in various haematological malignancies and has been verified by knockdown to sensitize myeloproliferative neoplasms (MPN) to DNA damaging agents. In this TEP we have expressed purified and determined the first ever crystal structures of RECQL5, in both APO and ADP/Mg2+ bound forms which crystallize in two distinctly different conformations. In vitro DNA stimulated ATPase assays suitable for high throughput screening have been developed as well as lower throughput orthogonal assays to verify potential hits. A fragment screening campaign has been initiated and single fragment hit has identified a potential allosteric site that may be targeted to block the transition between conformations that it thought to be part of the helicase mechanism. Finally included as part of the package we present 3 validated RECQL5 binding nanobodies, one of which is a potent inhibitor of RECQL5 ATPase activity and is suitable for use as a tool reagent to investigate inhibition of RECQL5 and its complexes in vitro.</p>
Human helicase RECQL5-Flag ChIP DNA from Saccharomyces cerevisiae strain W303-1AR5, asynchronous culture
GEO Series GSE180107. Saccharomyces cerevisiae. 4 samples. Type: Genome binding/occupancy profiling by genome tiling array.
Genome wide profiling of RNA Polymerase II upon knock-down or overexpression of RECQL5
GEO Series GSE49008. Homo sapiens. 9 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
RECQL5 Controls Transcript Elongation and Suppresses Transcription-Associated Genome Instability
GEO Series GSE49134. Homo sapiens. 25 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing.
Mapping of nascent RNA upon release of DRB in WT and KD of RECQL5
GEO Series GSE49133. Homo sapiens. 16 samples. Type: Expression profiling by high throughput sequencing.
Expression data from Saccharomyces cerevisiae strains expressing human RECQL5 helicase
GEO Series GSE180112. Schizosaccharomyces pombe; Saccharomyces cerevisiae. 4 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.