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126 results for “RNA interference”
Data for Falgenhauer, et al., "Transcriptional interference in toehold switch-based RNA circuits"
<p>Contains DNA sequences of the gene specific primers for RT-qPCR, the RT-qPCR raw data and output files used in "Transcriptional interference in toehold switch-based RNA circuits" by Falgenhauer et al.</p>
Fig. 6 in RNA interference mediated serine protease gene (Spbtry1) knockdown affects growth and mortality in the soybean pod borer (Lepidoptera: Olethreutidae)
Fig. 6. The mortality of the larvae feed on an artficial diet with added dsRNA. (*Student's t-test, n = 3, P <0.05; **Student's t-test, n = 3, P <0.01).
Fig. 4 in RNA interference mediated serine protease gene (Spbtry1) knockdown affects growth and mortality in the soybean pod borer (Lepidoptera: Olethreutidae)
Fig. 4. Relatve trypsin gene (Spbtry1) expression levels as determined by qPCR at different tme points. Actn was used as an internal reference gene. (*Student's t-test, n = 3, P <0.05; **Student's t-test, n = 3, P <0.01).
Fig. 5 in RNA interference mediated serine protease gene (Spbtry1) knockdown affects growth and mortality in the soybean pod borer (Lepidoptera: Olethreutidae)
Fig. 5. Effect of Spbtry1 RNAi on Leguminivora glycinivorella larval development. (A) The body weight of larvae fed on an artficial diet with added dsRNA at different tme points. (B) Pictures of the larvae showing reduced body size and developmental stage afer 15 days on an artficial diet with added dsRNA. (*Student's t-test, n = 3, P <0.05; **Student's t-test, n = 3, P <0.01).
Fig. 3. A in RNA interference mediated serine protease gene (Spbtry1) knockdown affects growth and mortality in the soybean pod borer (Lepidoptera: Olethreutidae)
Fig. 3. A) Relatve Spbtry1 gene expression levels was determined by qPCR (histograms) and RT-PCR (gel pictures) in the synganglion (SY), cutcle (CU), salivary (SA), midgut (MG), ovary (OV), tests (TE), and fat body (FT) in the 3rd instar soybean pod borer larvae. Actn was used as an internal reference gene. (B) Relatve trypsin gene (Spbtry1) expression levels as determined by qPCR (histograms) and RT-PCR (gel pictures) in soybean pod borer eggs (EG), 1st (N1), 2nd (N2), 3rd (N3), 4th (N4) instar larvae and pupae (PU), and adults (AD). Actn was used as an internal reference gene. Relatve Spbtry1 gene expression was analyzed by MJ Optcon Monitor Sofware Version 3.1.
Fig. 2 in RNA interference mediated serine protease gene (Spbtry1) knockdown affects growth and mortality in the soybean pod borer (Lepidoptera: Olethreutidae)
Fig. 2. Phylogenetc tree analysis of Spbtry1 and 13 homologues of other lepidopteran trypsin- and chymotrypsin-like serine proteases. The phylogenetc tree analysis was performed using the neighbor-joining algorithm to estmate evolutonary distances in MEGA 6.method at a gap penalty of 10, a gap length penalty of 0.2, and a bootstrap value of 1,000 iteratons.
Optimized protocols for RNA interference in Macrostomum lignano.
<p>Microscopy raw data from figures and supplemental figures and videos from "Optimized protocols for RNA interference in Macrostomum lignano" article. </p><p>Zeiss LSM format (.lsm or czi) and tiff exportation (.tif). Videos converted to AVI format (.avi).</p>
The Aedes aegypti RNA interference response against Zika virus in the context of co-infection with dengue and chikungunya viruses
<p>This is the corresponding data of the Publication in Plos Neglected tropical diseases.</p> <p>Zika virus (ZIKV) is a mosquito-borne human-pathogenic arbovirus of the <em>Flaviviridae</em> family, genus <em>Flavivirus</em>. Other arboviruses, including dengue (DENV) or chikungunya (CHIKV) virus, can occur in the same regions as ZIKV and are also transmitted by <em>Aedes aegypti</em>. Notably, it has been shown that these viruses can co-infect this mosquito, andco-transmission occurs. Such processes may add to the serious public health issues already linked to those pathogens. Arbovirus infections in mosquitoes are controlled through an immune response called RNA interference (RNAi). It is however unknown whether immune responses changs when a mosquito is exposed to a co-infection of ZIKV with either DENV or CHIKV. In this study, we provide evidence that ZIKV co-infections with CHIKV or DENV are similarly well controlled by RNAi as single infections. These findings give new insights into the dynamics of arboviral co-infections in mosquito vectors that increase our understanding of co-infection scenarios during arbovirus outbreaks.</p>
Data from: RNA interference reveals that male nuptial gift proteins affect female behavior to increase male paternity share in decorated crickets
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Data from: Reverse genetics in the tidepool: knockdown of target gene expression via RNA interference in the copepod Tigriopus californicus
Reverse genetic tools are essential for characterizing phenotypes of novel genes and testing functional hypotheses generated from next-generation sequencing studies. RNA interference (RNAi) has been a widely used technique for describing or quantifying physiological, developmental or behavioural roles of target genes by suppressing their expression. The marine intertidal copepod Tigriopus californicus has become an emerging model for evolutionary and physiological studies, but this species is not amenable to most genetic manipulation approaches. As crustaceans are susceptible to RNAi-mediated gene knock-down, we developed a simple method for delivery of gene-specific double-stranded RNA that results in significant suppression of target gene transcription levels. The protocol was examined on five genes of interest, and for each, at least 50% knock-down in expression was achieved. While knock-down levels did not reach 100% in any trial, a well-controlled experiment with one heat-shock gene showed unambiguously that such partial gene suppression may cause dramatic changes in phenotype. Copepods with suppressed expression of heat-shock protein beta 1 (hspb1) exhibited dramatically decreased tolerance to high temperatures, validating the importance of this gene during thermal stress, as proposed by a previous study. The application of this RNAi protocol in T. californicus will be invaluable for examining the role of genes putatively involved in reproductive isolation, mitochondrial function and local adaptation.
Data from: Reverse genetics in the tidepool: knockdown of target gene expression via RNA interference in the copepod Tigriopus californicus
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Fig. 1 in RNA interference mediated serine protease gene (Spbtry1) knockdown affects growth and mortality in the soybean pod borer (Lepidoptera: Olethreutidae)
Fig. 1. Nucleotde and deduced amino acid sequences of Spbtry1 cDNA cloned from Leguminivora glycinivorella. The numbers on the lef and right refer to the amino acid sequence. The putatve signal peptde of 17 amino acids is in bold and the cleavage site is indicated by an arrow. The cleavage site for the putatve propeptde removal is indicated by an arrowhead. The stop codon TAA is indicated by an asterisk. The putatve polyadenylaton signal (AATAA) is underlined. The putatve serine protease conserved motf (GDSGGPL) is boxed. The 3 amino acid residues (His72, Asp118, and Ser216) of the catalytc triad for serine protease actvity are indicated with white letters on a gray background. GenBank accession no. JQ340915.
Data from: Symbiont-mediated RNA interference in insects
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piRNAs and transgenerational RNA interference protect essential genes from RNA silencing in C. elegans
GEO Series GSE63114. Caenorhabditis elegans. 10 samples. Type: Non-coding RNA profiling by high throughput sequencing.
A mutant Bcl11b protein interferes with Bcl11a function during T lymphocyte and neuronal development [RNA-seq]
GEO Series GSE221764. Mus musculus. 11 samples. Type: Expression profiling by high throughput sequencing.
An Improved SNAP-ADAR Tool Enables Efficient RNA Base Editing to Interfere with Post-translational Protein Modification
GEO Series GSE264114. Homo sapiens. 26 samples. Type: Expression profiling by high throughput sequencing.
Small RNA genomics of Aedes aegypti mosquitoes discovers infectious viruses that trigger an RNA interference response [miRNA-seq]
GEO Series GSE309873. Aedes albopictus; Aedes aegypti. 209 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway
GEO Series GSE18806. Drosophila melanogaster. 11 samples. Type: Non-coding RNA profiling by high throughput sequencing; Other.
RNA-seq analysis of the transcriptional profile of SiHa cells with CNN3 interference.
GEO Series GSE134596. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
CRISPR activation and interference screens decode stimulation responses in primary human T cells [Bulk RNA-seq]
GEO Series GSE174284. Homo sapiens. 16 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.