Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
22
datasets available to search
ShareScore release 0.9.0
Dataset results
22 results for “RT-qPCR”
RT-qPCR dCT values of TLRs, NLRs and cytokines
<p>RT-qPCR dCt values of macrophages incubated with three <em>Lb. plantarum</em> strains. Values are normalized to the expression levels of ß-actin; of NLRs (<em>Nod1</em> and <em>Nod2</em>), TLRs (<em>Tlr2</em>, <em>Tlr4</em>, <em>Tlr5</em>, and <em>Tlr9</em>) and cytokines (<em>Ifn-</em><em>g</em>, <em>Il-10</em>, <em>Il</em>-6 and <em>Tnf-</em><em>a</em>)</p>
Denaturing and dNTPs reagents improve SARS-CoV-2 detection via single and multiplex RT-qPCR
<p>The datas correspond to article entitled: "Denaturing and dNTPs reagents improve SARS-CoV-2 detection via single and multiplex RT-qPCR". </p> <p>The file entitle GISAID have the fasta formats for 107259 genomes from the SARS-CoV-2 GISAID database from January to December 2020. Three documents in plane tex correspond:<br> sequences.fasta contain the original data.<br> sequences_clean.fasta. Corresponds to genomes sequences without nucleotides undeterminateds indicates with "N" in previous document.<br> alignment_clean.fasta. Contain the genomes sequences cleaned alingment. </p> <p>The file entitle GenBank have the data set from 19317 genomes from the SARS-CoV-2 GenBank database from January to October 2020 and the documets have the prrevious order.</p>
RNA-seq and RT-qPCR data showing MDF role in RNA splicing and gene expression control in Arabidopsis
<p>Plants respond to environmental stresses through controlled stem cell maintenance and meristem activity. One level of transcriptional control is RNA alternative splicing. However the mechanistic link between stress, meristem function and RNA splicing is poorly understood. The MERISTEM-DEFECTIVE (MDF)/DEFECTIVELY ORGANIZED TRIBUTARIES (DOT2) gene of Arabidopsis encodes a SR-related family protein, required for meristem function and leaf vascularization, and is the likely orthologue of the human SART1 and yeast snu66 splicing factors. MDF is required for the correct splicing and expression of key transcripts associated with root meristem function. We identified RSZ33 and ACC1, both known to regulate cell patterning, as splicing targets required for MDF function in the meristem. MDF expression is modulated by osmotic and cold stress, associated with differential splicing and specific isoform accumulation and shuttling between nucleus and cytosol, and acts in part via a splicing target SR34. We propose a model in which MDF controls splicing in the root meristem to promote stemness and repress stress response and cell differentiation pathways.</p>
RNA-seq and RT-qPCR data showing MDF role in RNA splicing and gene expression control in Arabidopsis
Open the record for dataset details and reuse information.
Data from: Seasonal dynamics and co-occurrence patterns of honey bee pathogens revealed by high-throughput RT-qPCR analysis
The health of the honey bee Apis mellifera is challenged by introduced parasites that interact with its inherent pathogens and cause elevated rates of colony losses. To elucidate co-occurrence, population dynamics and synergistic interactions of honey bee pathogens, we established an array of diagnostic assays for a high-throughput qPCR platform. Assuming that interaction of pathogens requires co-occurrence within the same individual, single worker bees were analyzed instead of collective samples. Eleven viruses, four parasites and three pathogenic bacteria were quantified in more than one thousand single bees sampled from sixteen disease-free apiaries in Southwest Germany. The most abundant viruses were Black Queen Cell Virus (84%), Lake Sinai Virus 1 (42%), and Deformed Wing Virus B (35%). Forager bees from asymptomatic colonies were infected with two different viruses in average, and simultaneous infection with four to six viruses was common (14%). Also the intestinal parasites Nosema ceranae (96%) and Crithidia mellificae/Lotmaria passim (52%) occurred very frequently. These results indicate that low-level infections in honey bees are more common than previously assumed. All viruses showed seasonal variation, while N. ceranae did not. The foulbrood bacteria Paenibacillus larvae and Melissococcus plutonius were regionally distributed. Spearman's correlations and multiple regression analysis indicated possible synergistic interactions between the common pathogens, particularly for Black Queen Cell Virus. Beyond its suitability for further studies on honey bees, this targeted approach may be, due to its precision, capacity and flexibility, a viable alternative to more expensive, sequencing-based approaches in non-model systems.
Sequences of primers used for RT-qPCR assay
<p>the detailed sequences of the primers of tsRNAs and mRNAs used for RT-qPCR assay</p>
A Open One-Step RT-qPCR for SARS-CoV-2 detection
<p>Sequences of the plasmids needed to purify the required enzymes for a standardized One-Step open RT-qPCR protocol to detect SARS-CoV-2 RNA in clinical samples. Supplementary Information</p>
COVID-19 Tests: Nasopharyngeal Swab vs Saliva Collected With Salivette® Cortisol for Viral RNA Sampling for RT-qPCR
ClinicalTrials.gov study NCT04599959. IPD Sharing: NO. Countries: 1. Publications: 1.
Data from: Seasonal dynamics and co-occurrence patterns of honey bee pathogens revealed by high-throughput RT-qPCR analysis
Open the record for dataset details and reuse information.
Supplementary Table S1. Primer sequence information for RT-qPCR used in this study.
Open the record for dataset details and reuse information.
Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma
GEO Series GSE209568. Homo sapiens. 2 samples. Type: Non-coding RNA profiling by high throughput sequencing.
RT-qPCR analysis in zebrafish Viperin KO fish under VHSV infections
GEO Series GSE250036. Danio rerio. 20 samples. Type: Expression profiling by RT-PCR.
RT-qPCR of metabolite and ICB exposed organoids
GEO Series GSE196268. Mus musculus. 6 samples. Type: Expression profiling by RT-PCR.
Benchmarking of RNA-sequencing analysis workflows using whole-transcriptome RT-qPCR expression data
GEO Series GSE83402. Homo sapiens. 4 samples. Type: Expression profiling by high throughput sequencing.
Identification of Circulating Fibrocytes and Dendritic Derivatives in Corneal Endothelium of Patients with Fuchs' Dystrophy [RT-qPCR array CAPH10410]
GEO Series GSE75675. Homo sapiens. 15 samples. Type: Expression profiling by RT-PCR.
RT-qPCR analysis of apoptosis related genes in patient-derived xenografts of small intestine neuroendocrine tumors in Balb/c NM after administration of Lu-177-octreotate, Lu-177-octreotate + A1M and A
GEO Series GSE200449. Homo sapiens. 22 samples. Type: Expression profiling by RT-PCR.
Identification of Circulating Fibrocytes and Dendritic Derivatives in Corneal Endothelium of Patients with Fuchs' Dystrophy [RT-qPCR array CAPH10409]
GEO Series GSE75674. Homo sapiens. 14 samples. Type: Expression profiling by RT-PCR.
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR
GEO Series GSE80332. Homo sapiens. 60 samples. Type: Expression profiling by RT-PCR.
QuantifyHER: Quantitative Immunofluorescence and/or RT-qPCR for Measuring HER2 in HER2-low Metastatic Breast Cancer
ClinicalTrials.gov study NCT06551116. IPD Sharing: Not stated. Countries: 1. Publications: 0.
The Type I interferon response impairs differentiation potential of pluripotent stem cells [RT-qPCR]
GEO Series GSE122793. Homo sapiens. 8 samples. Type: Expression profiling by RT-PCR.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.