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Tiller size measured on intact shoots in 1983 for the 1980-82 Eriophorum vaginatum reciprocal transplant experiment

These data were collected in August 1983 for tussocks transplanted in 1980-82 in a reciprocal transplant experiment and harvested in 2011. Important variables are garden name, source population, the number of green leaves, and the length of the longest leaf.

Tiller size measured on intact shoots in 1993 for the 1980-82 Eriophorum vaginatum reciprocal transplant experiment

These data were collected in July 1993 for tussocks transplanted in 1980-82 in a reciprocal transplant experiment and harvested in 2011. Important variables are garden name, source population, the number of green leaves, and the length of the longest leaf.

Tiller size measured on intact shoots in 2010 for the 1980-82 Eriophorum vaginatum reciprocal transplant experiment

These data were collected in July 2010 for tussocks transplanted in 1980-82 in a reciprocal transplant experiment and harvested in 2011. Important variables are garden name, source population, the number of old leaves, the number of new leaves, and the length of the longest two leaves.

Tussock survival from 1980 through 2010 for the 1980-82 Eriophorum vaginatum reciprocal transplant experiment

These data were collected in July 2010 for tussocks transplanted in 1980-82 in a reciprocal transplant experiment and harvested in 2011. Important variables are garden name, source population, and whether the tussocks were alive in 1983,1993,2009, and 2010.

Somatal length and density in 2010 for the 1980-82 Eriophorum vaginatum reciprocal transplant experiment

These data were collected in July 2010 for tussocks transplanted in 1980-82 in a reciprocal transplant experiment and harvested in 2011. Important variables are garden name, source population, length and density of stomata, and the temperature of tussocks.

Temperature response of dark respiration from the 1980-82 Eriophorum vaginatum reciprocal transplant experiment along Dalton Highway, Alaska.

These data were collected in July 2011 for tussocks transplanted in 1980-82 in a reciprocal transplant experiment and harvested in 2011. Important variables are garden name, source population, and dark respiration.

opencc-zeroJan 2020View details →

Disentangling the effects of methanogen community and environment on peatland greenhouse gas production by a reciprocal transplant experiment

1. Northern peatlands consist of a mosaic of peatland types that vary spatially and temporally and differ in their methane (CH4) production. Microbial community composition and environment both potentially control the processes that release carbon from anoxic peat either as CH4 or carbon dioxide (CO2), a less potent greenhouse gas than CH4. However, the respective roles of these controls remain unclear, which prevents incorporating microbes in the predictions of peatland CH4 emissions. 2. Here, a reciprocal transplant experiment was carried out to separate the influences of microbial community and environment in CH4 and anaerobic CO2 production. Peat from an acidic Sphagnum bog and a sedge fen with higher pH was enclosed in membrane bags with a pore size of 0.2 µm, preventing microbial colonization from the outside, and transplanted in the field for two months. 3. Potential CH4 production was primarily controlled by the environment. The conditions in the bog suppressed the initially higher activity of fen methanogens and reduced CH4 production by 79%. Against expectations, the inhibition was not specific to acetate-using Methanotrichaceae. Reciprocal transplantation favoured Methanosarcinaceae and potentially methylotrophic methanogenesis in general. Bog methanogens, mostly hydrogenotrophic Methanoregulaceae, retained their community structure and activity in the fen with a slight increase (+37%) in CH4 production. 4. Anaerobic CO2 production was controlled by both the microbial community and the environment. Transplantation led to increased CO2 production in both bog (+50%) and fen peat (+57%) with distinct bacterial community, showing that the new environment directed more carbon to other anaerobic processes than methanogenesis. 5. Taken together, these results relate differences in CH4 production of bogs and fens to ecophysiology of specific methanogen groups. The sensitiveness of fen methanogens to the acidic conditions in Sphagnum bogs can help explain the decrease of CH4 emission in the typical boreal peatland succession from young fens to older bogs. Increase in anaerobic CO2 vs. CH4 production with transplantation shows that disturbances of boreal peatlands can activate poorly defined pathways of anaerobic decomposition.

publicOct 2025View details →

Data from: Differences between two populations of Stipa krylovii in adaptive strategies: analysis of a three-year reciprocal transplant experiment

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publicSep 2025View details →

Data from: Reassessing adaptational lag in Eriophorum vaginatum: Short-term responses to reciprocal transplant and passive warming experiments in northern Alaska

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publicMay 2021View details →

Phenotypic, fitness and environmental data in support of the publication: selection favors adaptive plasticity in a long-term reciprocal transplant experiment

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publicJan 2020View details →

Disentangling the effects of methanogen community and environment on peatland greenhouse gas production by a reciprocal transplant experiment

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edi36/100

Effect of calcium addition on litter decomposition; data from reciprocal litter transplant experiment at Hubbard Brook, NH

Cross-site syntheses of litter decomposition studies have shown that litter calcium (Ca) concentration may have a role in controlling the extent of decomposition of tree foliage. We used an ongoing watershed CaSiO4 addition experiment at the Hubbard Brook Experimental Forest in New Hampshire, USA, to test the hypotheses that increased Ca in litter would have no effect on the initial rates of litter decay but would increase the extent or completeness (limit value) of foliar litter decomposition. We tested these hypotheses with a 6-year litter decomposition experiment using foliar litter of four tree species that are prominent at this site and in the Northern Hardwood forest type of North America: sugar maple (Acer saccharum Marsh), American beech (Fagus grandifolia Ehrh.) yellow birch (Betula alleghaniensis Britt.) and white ash (Fraxinus americana L.). The experiment used a reciprocal transplant design with the Ca-treated watershed and a control site providing two sources of litter and two placement sites. This dataset includes mass loss and changes in nutrient content of litter over the six year period.

openCC (other)Mar 2019View details →

opencc-zeroDec 2015View details →

Data from: Measuring microbial fitness in a field reciprocal transplant experiment

Microbial fitness is easy to measure in the laboratory, but difficult to measure in the field. Laboratory fitness assays make use of controlled conditions and genetically modified organisms, neither of which are available in the field. Among other applications, fitness assays can help researchers detect adaptation to different habitats or locations. We designed a competitive fitness assay to detect adaptation of Saccharomyces paradoxus isolates to the habitat they were isolated from (oak or larch leaf litter). The assay accurately measures relative fitness by tracking genotype frequency changes in the field using digital droplet PCR (DDPCR). We expected locally adapted S. paradoxus strains to increase in frequency over time when growing on the leaf litter type from which they were isolated. The DDPCR assay successfully detected fitness differences among S. paradoxus strains, but did not find a tendency for strains to be adapted to the habitat they were isolated from. Instead, we found that the natural alleles of the hexose transport gene we used to distinguish S. paradoxus strains had significant effects on fitness. The origin of a strain also affected its fitness: strains isolated from oak litter were generally fitter than strains from larch litter. Our results suggest that dispersal limitation and genetic drift shape S. paradoxus populations in the forest more than local selection does, although further research is needed to confirm this. Tracking genotype frequency changes using DDPCR is a practical and accurate microbial fitness assay for natural environments.

opencc-zeroDec 2010View details →

Data from: Modeling intraspecific adaptation of Abies sachalinensis to local altitude and responses to global warming, based on a 36-year reciprocal transplant experiment

Intraspecific adaptation in Abies sachalinensis was examined using models based on long-term monitoring data gathered during a reciprocal transplant experiment with eight seed source populations and six transplantation sites along an altitudinal gradient. The consequence of local adaptation was evaluated by testing the home-site advantage for upslope and downslope transplants at five ages. The populations' fitness-linked trait was set as their productivity (tree height ×survival rate) at each age. The effects of global warming were evaluated on the basis of the 36-year performance of downslope transplants. Evidence was found for adaptive genetic variation affecting both height and survival from an early age. Increasing the distance between seed source and planting site significantly reduced productivity for both upslope and downslope transplantation, demonstrating the existence of a significant home-site advantage. The decrease in productivity was most distinct for upslope transplantations, indicating strong local adaptation to high altitudes. Global warming is predicted to increase the productivity of high-altitude populations. However, due to their existing local adaptation, all tested populations exhibited lower productivity under warming than demes that were optimal for the new climate. These negative predictions should be considered when planning the management of locally-adapted plant species such as A. sachalinensis.

opencc-zeroDec 2014View details →

Data from: Variation of xylem vessel diameters across a climate gradient: insight from a reciprocal transplant experiment with a widespread boreal tree

Xylem vessel diameters represent an important plant hydraulic trait to ensure sufficient water supply from the roots to the leaves. The ability to adjust the hydraulic pathway to environmental cues is key in order to satisfy transpirational demands and maximize growth and survival. We evaluated the variability of vessel diameters in trembling aspen in a reciprocal transplant experiment. We tested six provenances from three ecological regions of North America planted at four test sites in western Canada. All test sites were established at the same time with the same provenances arranged in a randomized complete block design. Vessel diameter showed a strong interaction of population and test site suggesting a high degree of phenotypic plasticity in this trait. Gaussian kernel density estimates support plastic as well as genetic contributions in vessel diameter control trending from bimodal distributions at the most northern test site towards unimodal distributions at the warmest and mildest test site. Furthermore, we used test site-specific climate data in form of a 2-year, 5-year and 10-year average of 21 directly and derived climatic variables and found that average site-specific vessel diameters correlated strongly with summer moisture availability. A previously found negative relationship with vessel diameter and tree height in central Alberta was also found at two other boreal test sites but reversed at a wetter and milder sub-boreal test site. In summary, vessel diameters were highly plastic in response to different environments and varied with summer moisture availability. The variability of vessel diameter and tree height correlations suggests that vessel diameter alone cannot serve as a reliable proxy for long-term growth performance beyond boreal environments. Instead, selecting aspen populations with a high degree of plasticity in this trait appears to be the safest option for assisted migration and seed transfer programmes under climate change.

opencc-zeroAug 2021View details →

Survival and growth of Symphonia seedlings in a reciprocal transplantation experiment

Trees are characterised by the large number of seeds they produce. Although most of those seeds will never germinate, plenty will. Of those which germinate, many die young, and eventually only a minute fraction will grow to adult stage and reproduce. Is this just a random process? Do variations in germination and survival at very young stages rely on variations in adaptations to microgeographic heterogeneity? and do these processes matter at all in determining tree species distribution and abundance? We have studied these questions with the Neotropical Symphonia tree species. In the Guiana shield, Symphonia are represented by at least two sympatric taxa or ecotypes, Symphonia globulifera found almost exclusively in bottomlands, and a yet undescribed more generalist taxon/ecotype, Symphonia sp1. A reciprocal transplantation experiment (510 seeds, 16 conditions) was set up and followed over the course of 6 years to evaluate the survival and performance of individuals from different ecotypes and provenances. Germination, survival, growth, and herbivory showed signs of local adaptation, with some combinations of ecotypes and provenances growing faster and surviving better in their own habitat or provenance region. S. globulifera was strongly penalised when planted outside its home habitat but showed the fastest growth rates when planted in its home habitat, suggesting it's a specialist of a high-risk high-gain strategy. Conversely, S. sp1 behaved as a generalist, performing well in a variety of environments. The differential performance of seeds and seedlings in the different habitats matches the known distribution of both ecotypes, indicating that environmental filtering at the very early stages can be a key determinant of tree species distributions, even at the microgeographic level and among very closely related taxa. Furthermore, such differential performance also contributes to explain, in part, the maintenance of the different Symphonia ecotypes living in intimate sympatry despite occasional gene flow.

publicOct 2011View details →

Data from: Modeling intraspecific adaptation of Abies sachalinensis to local altitude and responses to global warming, based on a 36-year reciprocal transplant experiment

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