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Fig. 8 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 8. Rhagoletis merzi sp. n. paratypes (SIZK): male (a–с) and female (d–g): a, b — epandrium, hypandrium and surstyli (a — left, b — posterior), c — phallus glans; d — aculeus apex, e — aculeus, f — spermatheca; g — eversible membrane, ventral. Scale: d, f — 0.1 mm, e, g — 0.5 mm.

Fig. 6 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 6. Rhagoletis flavigenualis male (a–с) and female (d–g): a, b — epandrium, hypandrium and surstyli (a — left, b — posterior), c — phallus glans; d — aculeus apex, e — aculeus, f — spermatheca; g — eversible membrane, ventral. Scale: d, f — 0.1 mm, e, g — 0.5 mm.

Fig. 5 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 5. Rhagoletis flavigenualis male (a) and female (b–e): a–b — habitus left, c — abdomen dorsal; d — occiput and mesonotum, posterodorsally.

Fig. 7 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 7. Rhagoletis merzi sp. n. paratypes (MNHG): male (a–b) and female (c–d): a, c — habitus left, b, d — same, dorsal (photos by Bernard Landry).

Fig. 3 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 3. Rhagoletis bagheera paratype male (a, с–e) and female (b, f–h): a — habitus left (abdomen dissected), b — same, dorsal; c, d — epandrium, hypandrium and surstyli (c — left, d — posterior), e — phallus glans; f — aculeus apex, g — ovipositor, h — spermatheca.

Fig. 2 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 2. Palearctic species of Rhagoletis species similar to R. merzi, epandrium and surstyli, posterior view (a–c) and spermatheca (d–f): a, d — R. batava; b, e — R. flavigenualis; c, f — R. merzi sp. n.

Fig. 1 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 1. Palearctic species of Rhagoletis species similar to R. merzi, wings: a — R. zernyi; b — R. flavigenualis; c — R. merzi, sp. n.; d — R. bagheera; e — R. batava. Bands are marked as follows: A — apical, D — discal, SA — subapical, SB — subbasal. Red arrow shows connection of D and SA; cyan arrow shows cr — crescentic hyaline area. Scale: 1 mm.

Fig. 9 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 9. Bayesian phylogeny of Rhagoletis inferred from an alignment of 4270 bp of five genes (COI, CAD, ribosomal 28S, period and AATS) using MrBayes, and Maximum-Likelihood using MEGA 11. The first number on each branch is the bootstrap support from ML analysis; the second number represents posterior probability from Bayesian inference (BI). Asterisks (*) over branches indicate a Bayesian posterior probability of 1.0 and 100 % bootstrap support for the clade. Dash (- /) means that the clade inferred by MrBayes was not recovered by the ML analysis. Grey background shows position of the "core Nearctic taxa" (Smith et al., 2006). The bold numbers and color rectangles indicate species groups as follows: 1 — alternata group, 2 — cerasi group, 3 — cluster of ferruginea +nova + striatella groups, 4 — meigenii group, 5 — cingulata group, 6 — suavis group, 7 — ribicola group (sensu Bush, 1966), 8 — pomonella group, 9 — tabellaria group, 10 — juniperina group. Abbreviations: NA — Nearctic Region, PA — Palaearctic Region. Inlay shows relationships within the juniperina group.

Fig. 1 in Development of Rhagoletis pomonella and Rhagoletis indifferens (Diptera: Tephritidae) in mango and other tropical and temperate fruit in the laboratory

Fig. 1. Mean numbers of apple-origin female and male adults of Rhagoletis pomonella that landed on apple and tropical fruit in the laboratory in 2014. (A) Test using 1 female and 1 male per replicate; (B) test using 3 females and 3 males per replicate; n = number of replicates. Error bars represent SE. Ranks inside parentheses above bars with the same letter within sexes are not significantly different (P> 0.05; LSD test afer Kruskal–Wallis test). Females in (A): χ2 = 20.46; df = 1; P <0.0001; males in (A): χ2 = 20.37; df = 1; P <0.0001; females in (B): χ2 = 22.18; df = 4; P = 0.0002; males in (B): χ2 = 35.54; df = 4; P <0.0001.

Fig. 3 in Attraction of Rhagoletis indifferens (Diptera: Tephritidae) to white light in the presence and absence of ammonia

Fig. 3. Mean numbers ± SE of Rhagoletis indifferens flies caught on clear styrene trap with 60 W halogen bulb and no ammonia lure at different light intensities (lx) shown along the x-axis. Light intensities inside test cage were 90 to 250 lx. Maximum number of flies was 60. Means with same letters are not significantly different (P> 0.05, Tukey's HSD test).

Fig. 4 in Attraction of Rhagoletis indifferens (Diptera: Tephritidae) to white light in the presence and absence of ammonia

Fig. 4. Mean numbers ± SE of Rhagoletis indifferens flies caught on sticky yellow traps with ammonia lure in control (no heat) and heat treatments, using a painted 75 W halogen bulb: (A) 76.3 °C black bulb; (B) 105.8 °C black bulb; (C) 65.0 °C white bulb; (D) 90.5 °C white bulb. Light was provided to each trap by using a 45 W LED. Top temperature inside bar is that on the bulb surface; lower temperature is that at 2.5 cm from the bulb. Light intensities inside the test cage were 90 to 250 lx. Maximum number of flies was 60.

Fig. 2 in Attraction of Rhagoletis indifferens (Diptera: Tephritidae) to white light in the presence and absence of ammonia

Fig. 2. Mean numbers ± SE of Rhagoletis indifferens flies caught on sticky yellow traps with no ammonia lure at different light intensities (lx) shown along the x-axis: (A) experiment 3, 60 W halogen bulb and (B) experiment 4, 60 W LED bulb. Light intensities inside test cage were 90 to 250 lx. Maximum number of flies was 60. Means with same letters are not significantly different (P> 0.05, Tukey's HSD test).

Fig. 1 in Attraction of Rhagoletis indifferens (Diptera: Tephritidae) to white light in the presence and absence of ammonia

Fig. 1. Mean numbers ± SE of Rhagoletis indifferens flies caught on sticky yellow traps with ammonia lure at different light intensities (lx) shown along the x-axis: experiment 1: (A) test 1A, 60 W halogen bulb; (B) test 1B, 75 W halogen bulb; experiment 2: (C) test 2A, 60 W LED bulb; (D) test 2B, 60 W LED bulb. Light intensities inside test cage were 90 to 250 lx. Maximum number of flies was 60. Means with same letters are not significantly different (P> 0.05, Tukey's HSD test).

Fig. 5 in Attraction of Rhagoletis indifferens (Diptera: Tephritidae) to white light in the presence and absence of ammonia

Fig. 5. Mean numbers ± SE of Rhagoletis indifferens flies caught on sticky yellow traps with no ammonia lure: (A) experiment 7 using a black bulb: control (light off) and heated (light on); (B) experiment 8 using an iron: control (iron off) and heated (iron on). No bulb light was provided in either experiment. Top temperature above bar is that on the surface; lower temperature is that at 2.5 cm from the heat source. Light intensities inside the test cage were 90 to 250 lx. Maximum number of flies was 60.

opencc-by-4.0May 2019View details →

Fig. 1. Circular histogram rose plot showing Rhagoletis brncici and R in Differences in larval emergence chronotypes for sympatric Rhagoletis brncici Frías and Rhagoletis conversa (Bréthes) (Diptera, Tephritidae)

Fig. 1. Circular histogram rose plot showing Rhagoletis brncici and R. conversa larval emergence from fruit in a 24-h clock. For both graphs, the arrow shows the mean time of emergence. Main numbers in sections correspond to time in hours. Numbers accompanying circumferences refer to number of individual larvae replicates.

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Data from: Can the genomics of ecological speciation be predicted across the divergence continuum from host races to species? A case study in Rhagoletis

Open the record for dataset details and reuse information.

publicAug 2020View details →

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Identifying diagnostic genetic markers for a cryptic invasive agricultural pest: a test case using the apple maggot fly, Rhagoletis pomonella (Diptera: Tephritidae)

Insect pests destroy ~15% of all USA crops, resulting in losses of $15 billion annually. Thus, developing cheap, quick and reliable methods for detecting harmful species is critical to curtail insect damage and lessen economic impact. The apple maggot fly, Rhagoletis pomonella (Diptera: Tephritidae), is a major invasive pest threatening the multibillion-dollar apple industry in the Pacific Northwest USA. The fly is also sympatric with a benign but morphologically similar and genetically closely related species, R. zephyria, which attacks non-commercial snowberry. Unambiguous species identification is essential due to a zero-infestation policy of apple maggot for fruit export. Mistaking R. zephyria for R. pomonella triggers unnecessary and costly quarantines, diverting valuable control resources. Here we develop and apply a relatively simple and cost-effective diagnostic approach using Illumina sequencing of double digest restriction-site associated DNA markers. We identified five informative single nucleotide polymorphisms (SNPs) and designed a diagnostic test based on agarose gel electrophoresis of restriction enzyme digested polymerase chain reaction amplification products (RFLPs) to distinguish fly species. We demonstrated the utility of this approach for immediate, one day species identification by scoring apple- and snowberry-infesting flies of known host plant identity, reared directly from 11 sites throughout Washington. However, if immediate diagnosis is not required, or hundreds to thousands of specimens must be assessed, then a direct Illumina-based sequencing strategy, similar to that used here for diagnostic SNP identification can be powerful and cost-effective. The genomic strategy we present is effective for R. pomonella and also transferable to many cryptic pests.

opencc-zeroDec 2020View details →

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Supplementary data for "A new species of Rhagoletis (Diptera: Tephritidae) from Switzerland..."

Supplementary data file with the alignement of the seguences used in the paper by S. V. Korneyev et al. 2022. A new species of Rhagoletis (Diptera: Tephritidae) from Switzerland, with discussion of its relationships within the genus. Zoodiversity, 56(1)

opencc-by-4.0Feb 2022View details →

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Fig. 9 in A New Species Of Rhagoletis (Diptera, Tephritidae) From Switzerland, With Discussion Of Its Relationships Within The Genus

Fig. 9. Bayesian phylogeny of Rhagoletis inferred from