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5 results for “Rhizobiales”
Fig. 3 in A report on 24 unrecorded bacterial species of Korea isolated in 2016, belonging to the orders Rhizobiales and Sphingomonadales in the class Alphaproteobacteria
Fig. 3. Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences showing the relationship between the strains isolated in this study and their relatives in the Sphingomonadales of the Alphaproteobacteria. Bootstrap values (expressed as percentages of 1000 replications) over 70% are shown at nodes for neighbour-joining, maximum parsimony, and maximum likelihood methods, respectively. Filled circles and open circles indicate that the corresponding nodes were recovered by all treeing methods and by two treeing methods, respectively. Bootstrap values (>70%) are shown at nodes. Bar, 0.02 substitutions per nucleotide position.
Fig. 1 in A report on 24 unrecorded bacterial species of Korea isolated in 2016, belonging to the orders Rhizobiales and Sphingomonadales in the class Alphaproteobacteria
Fig. 1. Transmission electron micrographs of the strains isolated in this study. Strains: 1, HMF4680; 2, Gsoil 1041; 3, YC4-26; 4, 16_S3_ F7; 5, HMF4666; 6, SG2-5; 7, Gsoil 531; 8, KH7a; 9, Gsoil 3034-1; 10, 16_S4_T9; 11, CAU 1113; 12, IMCC25650; 13, IMCC25649; 14, JMS-19; 15, HMF8222; 16, HMF6028; 17, MMS16-CNU530; 18, HMF6507; 19, CAU 1116; 20, EMML2331-1; 21, Gsoil 130; 22, Gsoil 187; 23, CAU 1064; 24, IMCC25648.
Data from: A microbiotic survey of lichen-associated bacteria reveals a new lineage from the Rhizobiales
This study uses a set of PCR-based methods to examine the putative microbiota associated with lichen thalli. In initial experiments, generalized oligonucleotide-primers for the 16S rRNA gene resulted in amplicon pools populated almost exclusively with fragments derived from lichen photobionts (i.e., Cyanobacteria or chloroplasts of algae). This effectively masked the presence of other lichen-associated prokaryotes. In order to facilitate the study of the lichen microbiota, 16S ribosomal oligonucleotide-primers were developed to target Bacteria, but exclude sequences derived from chloroplasts and Cyanobacteria. A preliminary microbiotic survey of lichen thalli using these new primers has revealed the identity of several bacterial associates, including representatives of the extremophilic Acidobacteria, bacteria in the families Acetobacteraceae and Brucellaceae, strains belonging to the genus Methylobacterium, and members of an undescribed lineage in the Rhizobiales. This new lineage was investigated and characterized through molecular cloning, and was found to be present in all examined lichens that are associated with green algae. There is evidence to suggest that members of this lineage may both account for a large proportion of the lichen-associated bacterial community and assist in providing the lichen thallus with crucial nutrients such as fixed nitrogen.
Fig. 2 in A report on 24 unrecorded bacterial species of Korea isolated in 2016, belonging to the orders Rhizobiales and Sphingomonadales in the class Alphaproteobacteria
Fig. 2. Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences showing the relationship between the strains isolated in this study and their relatives in the Rhizobiales of the Alphaproteobacteria. Bootstrap values (expressed as percentages of 1000 replications) over 70% are shown at nodes for neighbour-joining, maximum parsimony, and maximum likelihood methods, respectively. Filled circles and open circles indicate that the corresponding nodes were recovered by all treeing methods and by two treeing methods, respectively. Bootstrap values (>70%) are shown at nodes. Bar, 0.02 substitutions per nucleotide position.
Data from: A microbiotic survey of lichen-associated bacteria reveals a new lineage from the Rhizobiales
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