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14 results for “Riboswitch”

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zenodo44/100

Riboswitch-inspired toehold riboregulators for gene regulation in Escherichia coli

<p>This dataset comprises flow cytometry data accompanying a publication on the&nbsp;development of synthetic riboregulators.</p> <p>These riboregulators were&nbsp;inspired by the architecture of naturally occurring riboswitches and toehold-mediated strand displacement. Specifically, we adopt the toehold switch hairpin and inserted regulatory sequences within the loop region of which accessibility can be controlled by toehold-mediated strand displacement. We utilized this design principle to develop toehold translation repressor and toehold transcriptional repressor, which regulate mCherry expression in&nbsp;<em>E. coli&nbsp;</em>in translational and transcriptional levels with certain ON/OFF ratios. Furthermore, we combined these two riboregulators and developed them into a NOR gate switch that can regulate downstream GFP expression in&nbsp;<em>E. coli&nbsp;</em>with different input&nbsp;conditions of trigger RNA. We used flow cytometry to quantify the expression level of the NOR gate switch under different inputs.</p>

opencc-by-4.0Aug 2021View details →
zenodo44/100

Ha-SingleMoleculeLab's data for publication: Linking folding dynamics and function of SAM/SAH riboswitches at the single molecule level

<p>This upload is the raw data that support our findings sent to review on Nucleic Acids Research, corresponding to each individual figure. The paper title is &quot;&nbsp;Linking folding dynamics and function of SAM/SAH riboswitches at the single molecule level&quot;.</p>

opencc-by-4.0Jun 2023View details →
zenodo36/100

RNA Ensembles From Solvent Accessibility Data: Application to the SAM-I Riboswitch Aptamer Domain

<p>SASA-derived ensembles of the -SAM and +SAM states of the SAM-responsive riboswitch.</p>

opencc-by-4.0Dec 2020View details →
zenodo36/100

TECdisplay data for Clostridium beijerinckii pfl ZTP riboswitch variant libraries

<p>Transcription termination/antitermination efficiency data for the <em>Clostridium beijerinckii</em> <em>pfl</em> ZTP riboswitch variant libraries. Data were generated using the Transcription Elongation Complex Display (TECdisplay) procedure.</p>

opencc-by-4.0Sep 2024View details →
zenodo36/100

Linked multipoint cotranscriptional RNA chemical probing data for the E. coli SRP RNA, C. beijerinckii pfl ZTP riboswitch, and B. cereus crcB fluoride riboswitch

<p>Cotranscriptional RNA chemical probing data for the <em>E. coli</em> signal recognition particle (SRP) RNA, <em>Clostridium beijerinckii pfl</em> ZTP riboswitch, and <em>Bacillus cereus crcB </em>fluoride riboswitch. Data were generated using the linked multipoint Transcription Elongation Complex RNA structure probing (TECprobe-LM) and variable length Transcription Elongation Complex RNA structure probing (TECprobe-VL) procedures.</p>

opencc-by-4.0Sep 2024View details →
zenodo36/100

Cotranscriptional RNA chemical probing data for ZTP, fluoride, and ppGpp riboswitches

<p>Cotranscriptional RNA chemical probing data for the <em>Clostridium beijerinckii pfl</em> ZTP riboswitch, <em>Bacillus cereus crcB </em>fluoride riboswitch, and <em>Clostridiales bacterium oral taxon </em>876<em> </em>str. F0540 ppGpp riboswitch. Data were generated using the Transcription Elongation Complex RNA structure probing-multilength (TECprobe-ML) and single length (TECprobe-SL) procedures.</p>

opencc-by-4.0Feb 2023View details →
dryad36/100

Single-molecule and bulk fluorescence and biolayer interferometry on the NAD-II riboswitch and its interactions with NMN

Open the record for dataset details and reuse information.

publicDec 2024View details →
geo24/100

Sequestration of a two-component response regulator by a riboswitch regulated non-coding RNA

GEO Series GSE60363. Listeria monocytogenes EGD-e. 8 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenAug 2014View details →
geo24/100

Functional Validation of SAM Riboswitch Element A from Listeria monocytogenes

GEO Series GSE266872. Listeria monocytogenes. 4 samples. Type: Other.

openGEO-OpenMay 2025View details →
geo24/100

High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells

GEO Series GSE143466. Homo sapiens. 125 samples. Type: Other.

openGEO-OpenFeb 2020View details →
geo20/100

DANCE-MaP probing of the adenine riboswitch

GEO Series GSE182552. Vibrio vulnificus. 32 samples. Type: Other.

openGEO-OpenMar 2022View details →
geo20/100

Specific targeting of transcriptional T-box riboswitches leads to effective inhibition of S. aureus

GEO Series GSE300037. Staphylococcus aureus. 4 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenOct 2025View details →
geo20/100

Cyclic diguanylate regulates virulence factor genes via multiple riboswitches in Clostridium difficile

GEO Series GSE120198. Clostridioides difficile. 8 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenSep 2018View details →
geo16/100

A dual T-box riboswitch in Enterococcus faecium binds two tRNAs

GEO Series GSE161499. Enterococcus faecium. 8 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenNov 2020View details →

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International Brain Laboratory public data

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Last verified 2026-04-29Open record