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17 results for “Ruditapes philippinarum”
Fig 3 in In situ fast marking study of manila clams (Ruditapes philippinarum)
Fig 3: Magnification of a ventral margin in Manila clam: green fluorescent mark produced following 30 minutes calcein solution exposure (100 mg L−1) and observed growth increments before and after marking. Along a growth axis, grey levels have been measured (AU: arbitrary unit) and each grey peak is identified (blue circle) as the limit between each opaque band and each hyaline band, reflecting each one growth increment.
Fig 2 in In situ fast marking study of manila clams (Ruditapes philippinarum)
Fig 2: A Valve of Manila Clam with the line which represents the direction of growth (DOG); B) Thick section of shell (thickness of 0.2 mm); C) Ventral margin of a calcein-marked shell with a fluorescent mark (Calcein exposure time and concentration are indicated for each staining experiment)
Fig 1 in In situ fast marking study of manila clams (Ruditapes philippinarum)
Fig 1: In situ fast marking study of Manila Clam with the calcein fluorochrome dye.
SNP array for parentage assignment of the Manila clam, Ruditapes philippinarum
<p>The Manila clam <i>Ruditapes philippinarum</i>, a major cultured shellfish species, is threatened by infection with the microparasite <i>Perkinsus olseni</i>, whose prevalence increases with high water temperatures. Under the current trend of climate change, the already severe effects of this parasitic infection might rapidly increase the frequency of mass mortality events. Treating infectious diseases in bivalves is notoriously problematic, therefore selective breeding for resistance represents a key strategy for mitigating the negative impact of pathogens. A crucial step in initiating selective breeding is the estimation of genetic parameters for traits of interest, which relies on the ability to record parentage and accurate phenotypes in a large number of individuals. Here, to estimate the heritability of resistance against <i>P. olseni</i>, a field experiment mirroring conditions in industrial clam production was set up, a genomic tool was developed for parentage assignment, and parasite load was determined through quantitative PCR.</p> <p>A mixed-family cohort of potentially 1479 clam families was produced in a hatchery by mass spawning of 53 dams and 57 sires. The progenies were seeded in a commercial clam production area in the Venice lagoon, Italy, where high prevalence of <i>P. olseni</i> had previously been reported. Growth and parasite load were monitored every month and, after one year, more than 1000 individuals were collected and DNA and phenotype records.</p> <p>A 245-SNP panel was developed using candidate markers obtained from a pooled sequencing approach on two DNA samples from all the potential parents and from a Venice lagoon clam population. For 246 individuals of the mixed-family F1, sire and dam representation were high (75 and 85%, respectively), indicating a very limited risk of inbreeding. Moderate heritability (0.20 – 0.30) was estimated for growth traits, while parasite load showed high heritability, estimated at 0.52. No significant genetic correlations were found between growth-associated traits and parasite load.</p> <p>Overall, the study shows high potential for selecting clams resistant to parasite<i> </i>load<i>.</i> Breeding for resistance may help limit the negative effects of climate change on clam production, as the prevalence of the parasite is predicted to increase under a future scenario of higher temperatures. Finally, the limited genetic correlation between resistance and growth suggests that breeding programs could incorporate dual selection without negative interactions.</p>
Data from: Molecular and morphological evidence of hybridization between native Ruditapes philippinarum and the introduced Ruditapes form in Japan
Marine aquaculture and stock enhancement are major causes of the introduction of alien species. A good example of such an introduction is the Japanese shortneck clam Ruditapes philippinarum, one of the most important fishery resources in the world. To meet the domestic shortage of R. philippinarum caused by depleted catches, clams were imported to Japan from China and the Korean peninsula. The imported clam is an alien species that has a very similar morphology, and was misidentified as R. philippinarum (hereafter, Ruditapes form). We genotyped 1,186 clams of R. philippinarum and R. form at four microsatellite loci, sequenced mitochondrial DNA (COI gene fragment) of 485 clams, 34 of which were R. variegatus, and measured morphometric and meristic characters of 754 clams from 12 populations in Japan and China, including the Ariake Sea and Tokyo Bay, where large numbers of R. form were released. Our analyses confirmed that R. form was from the genus Ruditapes, and the genetic differentiation between R. philippinarum and R. form was distinct, but small, compared with five bivalve outgroups. However, R. form had distinct shell morphology, especially larger numbers of radial ribs on the shell surface, suggesting that R. form might be a new Ruditapes species or a variation of R. philippinarum that originated from southern China. A genetic affinity of the sample from the Ariake Sea to R. form was found with the intermediate shell morphology and number of radial ribs, and the hybrid proportion was estimated at 51.3 ± 4.6 % in the Ariake Sea.
Data from: Molecular and morphological evidence of hybridization between native Ruditapes philippinarum and the introduced Ruditapes form in Japan
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SNP array for parentage assignment of the Manila clam, Ruditapes philippinarum
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Sub-lethal effects of innovative anti-corrosion nanoadditives on the marine bivalve Ruditapes philippinarum
<div>The present dataset contains chemical, physicochemical, and ecotoxicological data upon exposure of the marine bivalve <em>Ruditapes philippinarum</em> to novel anti-corrosion nanomaterials (Zn-Al LDH-BTA and Zn-Al LDH-GCN) and their soluble counterparts (BTA-benzotriazole and GCN-gluconate). Chemical analyses data include the quantification of relevant anions (GCN or BTA, and chlorides, nitrates, nitrites determined through High-Performance Liquid Chromatography) and target metals (Zn and Al determined through Inductively Coupled Plasma Optical Emission Spectrometry). Ecotoxicological data includes physiological (condition index), biochemical (biomarkers), and genotoxic (comet assay) endpoints.</div>
Data from: Early replication dynamics of sex-linked mitochondrial DNAs in the doubly uniparental inheritance species Ruditapes philippinarum (Bivalvia Veneridae)
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Transcriptome analysis of Manila clam (Ruditapes philippinarum) digestive gland after exposure to different concentrations of ibuprofen.
GEO Series GSE38863. Ruditapes philippinarum. 60 samples. Type: Expression profiling by array.
Gene Expression Analysis of Ruditapes philippinarum Haemocytes after Experimental Perkinsus olseni Zoospore Challenge and Infection in the Wild
GEO Series GSE99162. Ruditapes philippinarum. 16 samples. Type: Expression profiling by array.
Toxicogenomic analyses of Ruditapes philippinarum by DNA microarray: new tools for anthropogenic impact assessment on the Venice Lagoon.
GEO Series GSE26985. Ruditapes philippinarum. 64 samples. Type: Expression profiling by array.
Biological replicates of Ruditapes philippinarum digestive gland pools
GEO Series GSE24050. Ruditapes philippinarum. 4 samples. Type: Expression profiling by array.
Comparison of gene expression profile of Ruditapes philippinarum sampled in Marghera and Alberoni
GEO Series GSE27194. Ruditapes philippinarum. 12 samples. Type: Expression profiling by array.
Gene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus challenge using an immune-enriched oligo-microarray
GEO Series GSE43274. Ruditapes philippinarum. 36 samples. Type: Expression profiling by array.
Comparison of gene expression profile of Ruditapes philippinarum gills and digestive glands.
GEO Series GSE24101. Ruditapes philippinarum. 6 samples. Type: Expression profiling by array.
Transcriptional changes in Manila clam (Ruditapes philippinarum) in response to brown ring disease
GEO Series GSE58198. Ruditapes philippinarum. 36 samples. Type: Expression profiling by array.
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